Click here to switch to the map view.

The map label for this gene is fadE25 [H]

Identifier: 85858066

GI number: 85858066

Start: 229522

End: 230676

Strand: Direct

Name: fadE25 [H]

Synonym: SYN_02586

Alternate gene names: 85858066

Gene position: 229522-230676 (Clockwise)

Preceding gene: 85858061

Following gene: 85858067

Centisome position: 7.22

GC content: 53.68

Gene sequence:

>1155_bases
ATGTACATCAACACGGAGACTGAAGATCTGAAAACCGCGATCGATGCGATACGCAAGGCAGTAAAGGATCGTATTGCTCC
CCTGGCCGCAGAGGTCGATGACTCGGGTGTTATCAAACCGGAAATATATGATCTTTTATGGGATCTGGGGCTCATGACCG
TGACCTATCCACCGGAATACGGCGGATCGGAAACTAATCCCGGGACACTTTTGTGCATAGGGTGTGAAGAGATAGCGAAA
GCCTGCGCTTCAACGGCCCTGCTGCTGATCATTCAGGCCGTGGGAAGTTTTCCCCTCATGCATGGCGGCAGAAAGGAACT
TCTTGATCGCATCGCGCCGAGGATCGTGAACAATCGTGAACTGGCCGGGTATCTGGTATCGGAACCCGGAGCCGGATCAG
ATGTGAAGGCGATTCGGACCAAGGCGGTCAAAGACGGCAACGACTGGGTTATCAACGGGACCAAGTGCTGGGCCACAAAT
GGGCCGATTGCTTCTTTCTATTCCTGCCTGTGCCGGACCAAGGATGATAAGGGAGTTCAGGGTTATTCCTTCTTCCTGGT
GGAACGAAACACCCCCGGACTGTCGGTTGGAAAAATCGAGCACAAAATGGGGATGAGGGGTTCCCAAACCTCCGAAGTGA
TACTTGAAGATGTGCGCGTGCCCGCGGAAAACCTGCTGGGTGAGTTGAACAACGGCTTCAAGCTCGCCATGAAGGATTTC
GACATGTCCCGTCCGGCTATCGCGGCGCAGGCCCTGGGAATTTCCGAGGGTGCTTTCGCGCAGATGGAGACCTATTCGAG
GGAACGGTATACCTTTGGCAAGCCTCTGTGCGAGCACGGGATGATCACTCAGATTATCGCCGACAGCGCGGCTCTGATCG
AGGCGGGAAGGGGTCTTATCTATCAGGCGGCGGATCTGTATGACAAGGGGAAGAAAAATACCAAATTGGCCTCCATGGCC
AAGTTCTTCATGGGCGATGCCGCCGTGAAAATCACCACCGATGCGATTCAGGTCTTTGGCGGATATGGCTACACGCATGA
TTATCCGGTGGAGCGCATGTTCCGGGATGCGAAACTGACCCAGATCTTTGAAGGGGCCAATCAAATTCAACGCATTGTCG
TTGCAAGGGAGATCCGTGATGAGCAATCAAAGTAA

Upstream 100 bases:

>100_bases
AAAAACAAAAGGAATTTTAGTAGGAATATCGGGTGGTTTGTTGGTAATTCATGGATCAATGTAAAACACAAAAGAATTTA
TATTAGGAGGGAGATAAACA

Downstream 100 bases:

>100_bases
TGATACCACAATCACTCAAAGGAGAGATACAATGAACGAACTTACCGAAGAGCAAAAACTTTTAATGGAAATGGTGCGCA
ATCTGGCGGTTAGGGAAATT

Product: acyl-CoA dehydrogenase

Products: 2,3-dehydroacyl-CoA; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MYINTETEDLKTAIDAIRKAVKDRIAPLAAEVDDSGVIKPEIYDLLWDLGLMTVTYPPEYGGSETNPGTLLCIGCEEIAK
ACASTALLLIIQAVGSFPLMHGGRKELLDRIAPRIVNNRELAGYLVSEPGAGSDVKAIRTKAVKDGNDWVINGTKCWATN
GPIASFYSCLCRTKDDKGVQGYSFFLVERNTPGLSVGKIEHKMGMRGSQTSEVILEDVRVPAENLLGELNNGFKLAMKDF
DMSRPAIAAQALGISEGAFAQMETYSRERYTFGKPLCEHGMITQIIADSAALIEAGRGLIYQAADLYDKGKKNTKLASMA
KFFMGDAAVKITTDAIQVFGGYGYTHDYPVERMFRDAKLTQIFEGANQIQRIVVAREIRDEQSK

Sequences:

>Translated_384_residues
MYINTETEDLKTAIDAIRKAVKDRIAPLAAEVDDSGVIKPEIYDLLWDLGLMTVTYPPEYGGSETNPGTLLCIGCEEIAK
ACASTALLLIIQAVGSFPLMHGGRKELLDRIAPRIVNNRELAGYLVSEPGAGSDVKAIRTKAVKDGNDWVINGTKCWATN
GPIASFYSCLCRTKDDKGVQGYSFFLVERNTPGLSVGKIEHKMGMRGSQTSEVILEDVRVPAENLLGELNNGFKLAMKDF
DMSRPAIAAQALGISEGAFAQMETYSRERYTFGKPLCEHGMITQIIADSAALIEAGRGLIYQAADLYDKGKKNTKLASMA
KFFMGDAAVKITTDAIQVFGGYGYTHDYPVERMFRDAKLTQIFEGANQIQRIVVAREIRDEQSK
>Mature_384_residues
MYINTETEDLKTAIDAIRKAVKDRIAPLAAEVDDSGVIKPEIYDLLWDLGLMTVTYPPEYGGSETNPGTLLCIGCEEIAK
ACASTALLLIIQAVGSFPLMHGGRKELLDRIAPRIVNNRELAGYLVSEPGAGSDVKAIRTKAVKDGNDWVINGTKCWATN
GPIASFYSCLCRTKDDKGVQGYSFFLVERNTPGLSVGKIEHKMGMRGSQTSEVILEDVRVPAENLLGELNNGFKLAMKDF
DMSRPAIAAQALGISEGAFAQMETYSRERYTFGKPLCEHGMITQIIADSAALIEAGRGLIYQAADLYDKGKKNTKLASMA
KFFMGDAAVKITTDAIQVFGGYGYTHDYPVERMFRDAKLTQIFEGANQIQRIVVAREIRDEQSK

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: COG1960

COG function: function code I; Acyl-CoA dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI187960098, Length=379, Percent_Identity=40.1055408970976, Blast_Score=275, Evalue=4e-74,
Organism=Homo sapiens, GI4557231, Length=379, Percent_Identity=40.1055408970976, Blast_Score=275, Evalue=5e-74,
Organism=Homo sapiens, GI4501859, Length=382, Percent_Identity=36.3874345549738, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI4557233, Length=369, Percent_Identity=37.6693766937669, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI226958412, Length=386, Percent_Identity=32.1243523316062, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI21361497, Length=356, Percent_Identity=33.1460674157303, Blast_Score=196, Evalue=5e-50,
Organism=Homo sapiens, GI4501857, Length=365, Percent_Identity=29.041095890411, Blast_Score=195, Evalue=5e-50,
Organism=Homo sapiens, GI226958414, Length=348, Percent_Identity=32.4712643678161, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI7656849, Length=383, Percent_Identity=31.5926892950392, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI76496475, Length=382, Percent_Identity=29.5811518324607, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI4557235, Length=382, Percent_Identity=29.5811518324607, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI4503943, Length=384, Percent_Identity=26.5625, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI7669494, Length=365, Percent_Identity=26.5753424657534, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI211971008, Length=261, Percent_Identity=31.4176245210728, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI48976061, Length=261, Percent_Identity=31.4176245210728, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI38505218, Length=399, Percent_Identity=26.5664160401003, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1786223, Length=350, Percent_Identity=26.5714285714286, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI87081958, Length=357, Percent_Identity=29.9719887955182, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI87082384, Length=275, Percent_Identity=25.4545454545455, Blast_Score=81, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17534899, Length=364, Percent_Identity=38.4615384615385, Blast_Score=255, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI17569725, Length=374, Percent_Identity=38.5026737967914, Blast_Score=255, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI17570075, Length=365, Percent_Identity=37.8082191780822, Blast_Score=252, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI17508101, Length=374, Percent_Identity=34.2245989304813, Blast_Score=224, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI17506239, Length=382, Percent_Identity=33.7696335078534, Blast_Score=218, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI17538396, Length=378, Percent_Identity=31.7460317460317, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17551932, Length=373, Percent_Identity=31.0991957104558, Blast_Score=174, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI71985184, Length=350, Percent_Identity=30.5714285714286, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17533517, Length=372, Percent_Identity=28.2258064516129, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17505929, Length=369, Percent_Identity=31.7073170731707, Blast_Score=161, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI71990804, Length=296, Percent_Identity=32.0945945945946, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI71982178, Length=273, Percent_Identity=31.1355311355311, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI32563615, Length=237, Percent_Identity=34.5991561181435, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17531909, Length=244, Percent_Identity=32.3770491803279, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17534353, Length=377, Percent_Identity=25.1989389920424, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71985192, Length=290, Percent_Identity=28.9655172413793, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17531913, Length=179, Percent_Identity=31.2849162011173, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI86563383, Length=298, Percent_Identity=25.8389261744966, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI86563381, Length=298, Percent_Identity=25.8389261744966, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17562446, Length=288, Percent_Identity=25.3472222222222, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24660351, Length=375, Percent_Identity=40.2666666666667, Blast_Score=283, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24646207, Length=374, Percent_Identity=38.2352941176471, Blast_Score=231, Evalue=6e-61,
Organism=Drosophila melanogaster, GI21356377, Length=366, Percent_Identity=37.431693989071, Blast_Score=225, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24666513, Length=377, Percent_Identity=33.6870026525199, Blast_Score=214, Evalue=9e-56,
Organism=Drosophila melanogaster, GI21355753, Length=374, Percent_Identity=32.3529411764706, Blast_Score=187, Evalue=8e-48,
Organism=Drosophila melanogaster, GI281363737, Length=363, Percent_Identity=31.9559228650138, Blast_Score=181, Evalue=9e-46,
Organism=Drosophila melanogaster, GI19920834, Length=280, Percent_Identity=28.2142857142857, Blast_Score=111, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.3

Molecular weight: Translated: 42064; Mature: 42064

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00073 ACYL_COA_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYINTETEDLKTAIDAIRKAVKDRIAPLAAEVDDSGVIKPEIYDLLWDLGLMTVTYPPEY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEECCCC
GGSETNPGTLLCIGCEEIAKACASTALLLIIQAVGSFPLMHGGRKELLDRIAPRIVNNRE
CCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCC
LAGYLVSEPGAGSDVKAIRTKAVKDGNDWVINGTKCWATNGPIASFYSCLCRTKDDKGVQ
CEEEEEECCCCCCCHHHHHHHHCCCCCCEEEECEEEEECCCCHHHHHHHHHCCCCCCCCC
GYSFFLVERNTPGLSVGKIEHKMGMRGSQTSEVILEDVRVPAENLLGELNNGFKLAMKDF
CEEEEEEECCCCCCEECHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHCCCCEEEEEECC
DMSRPAIAAQALGISEGAFAQMETYSRERYTFGKPLCEHGMITQIIADSAALIEAGRGLI
CCCCCHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCE
YQAADLYDKGKKNTKLASMAKFFMGDAAVKITTDAIQVFGGYGYTHDYPVERMFRDAKLT
EEHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHCCCCCCCCCCHHHHHHHHHHH
QIFEGANQIQRIVVAREIRDEQSK
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYINTETEDLKTAIDAIRKAVKDRIAPLAAEVDDSGVIKPEIYDLLWDLGLMTVTYPPEY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEECCCC
GGSETNPGTLLCIGCEEIAKACASTALLLIIQAVGSFPLMHGGRKELLDRIAPRIVNNRE
CCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCC
LAGYLVSEPGAGSDVKAIRTKAVKDGNDWVINGTKCWATNGPIASFYSCLCRTKDDKGVQ
CEEEEEECCCCCCCHHHHHHHHCCCCCCEEEECEEEEECCCCHHHHHHHHHCCCCCCCCC
GYSFFLVERNTPGLSVGKIEHKMGMRGSQTSEVILEDVRVPAENLLGELNNGFKLAMKDF
CEEEEEEECCCCCCEECHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHCCCCEEEEEECC
DMSRPAIAAQALGISEGAFAQMETYSRERYTFGKPLCEHGMITQIIADSAALIEAGRGLI
CCCCCHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCE
YQAADLYDKGKKNTKLASMAKFFMGDAAVKITTDAIQVFGGYGYTHDYPVERMFRDAKLT
EEHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHCCCCCCCCCCHHHHHHHHHHH
QIFEGANQIQRIVVAREIRDEQSK
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acceptor

Specific reaction: acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]