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The map label for this gene is bioK [H]

Identifier: 85858043

GI number: 85858043

Start: 205529

End: 206899

Strand: Direct

Name: bioK [H]

Synonym: SYN_02563

Alternate gene names: 85858043

Gene position: 205529-206899 (Clockwise)

Preceding gene: 85858042

Following gene: 85858044

Centisome position: 6.46

GC content: 57.62

Gene sequence:

>1371_bases
ATGAACGGACAGGAAGAAAAAAAAAGGCAATTGGTCGCCGACGGTCTGAAATCCATCTGGTTTCCCTTCACCCAGATGCA
GGAGTTTACCCCGGATGATGTACTGATCATCGCCGAGGGGAAAGGAGTTTTCCTTCGTGATGTGGACGGCAGGGATTACA
TCGATGGGGTTTCGTCTCTCTGGACCAATGTTCACGGCCATCGGAAGGAACAGATTGACCGGGCCATTCGGGAGCAACTG
AATCGGGTCGCCCATTCCACCCTGCTCGGCTTGGCCCATGAACCCGCCATTGTCTGTGCGCGGAAACTGCTGGAGATCGT
GCCGAAAGGGCTGACCCGGGTCTTTTACTCGGAAAGCGGCTCCACCGCTGTGGAAATCGCCTTGAAGATGGCCTTTCAGT
ATCAGCAGCAGGCAGCCGGAGGCGATCCCCGCAAAATCCGTTTCATGTCCCTGACCAACGCTTACCACGGCGATACCCTG
GGTTCGGTCAGTGCGGGCGGGATCGACCTGTTTCATGCCACCTACCGGGAACTTCTTTTTCCGACGGTCAAGGCCGCAGC
GCCGTATTGCTACCGATGTCCATTCGGAGCTTCCTATCCAGGCTGCCGGTTTGACTGCCTGAGTCAGCTGGAAAACCTGC
TGGCAGCTCACGCCCATGAACTGGCGGCCCTGATTATCGAACCCCTCGTTCAGGGGGCGGCTGGCATTCTGGTTCAGCCT
CCCGGGTATCTGAAACGGGTTCGGGAGCTCTGCACGCGTTACGGGGTGCTGATGATTGCCGATGAAGTGGCGGTAGGCTT
CGGTAAGACAGGCAGGATGTTTGCCTGTGAACACGAAGACGTTGCCCCGGACATTCTGGCCCTGGGCAAGGGGCTGTCCG
GAGGATATCTGCCCCTTGCGGCTACTGTGGCGACAGAAGAAATCTATAAGGGGTTTCTTGCCCGCTTCGACGAGTTCAAG
ACCTTTTTCCACGGCCATACCTACACGGGGAATCCCCTGGCCTGCGCCGCCGCCATAGCGAATCTGGACGTCTTTGCCGA
AGAGCGGGTCATCGAGGGATCAGGCGAAAAAATTGAACGGCTGACCCAGGGGCTGACGCCGATTGCTTCCTTGAATCATG
TGGGGGAGGTCCGTCAGCGGGGATTCATGGTCGGGATCGAACTCGTGGCGGACAGGGAGACTAAGGCGCTGTATCCTCCA
GCCGAACGAATCGGGCATAAAGTCATCCTGGAGGCGCGGAAACGGGGGCTGATTATCCGCCCGCTGGGAGATGTGATTGT
CCTTATGCCGCCTTTGTGCATCACGACAGAAGAAATCGATCGCCTTTGCGAAATCACGTATGAGTCCATCCGGGCCGTTG
CGGGGGAATAG

Upstream 100 bases:

>100_bases
AGCTTCCGTTTTACCCCACCATAGTAAAGGGCTGAGGCCGGGCGATTCTCACGAAGGCGCGCAAATCTGAAACTTTGGAA
AAAGATTGGAGAAAAGGGCA

Downstream 100 bases:

>100_bases
GGAAAATGCCGAAATCGCCGGACCGGGGGAAGGGGGAAGTCCCCGAGAAATCGGAAGATTCCGTTATCGTTTTCTCCGGG
GTCTTTTATTTCGATCCCGC

Product: adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Products: S-adenosyl-4-methylthio-2-oxobutanoate; 7,8-diaminononanoate

Alternate protein names: 7,8-diamino-pelargonic acid aminotransferase; DAPA aminotransferase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MNGQEEKKRQLVADGLKSIWFPFTQMQEFTPDDVLIIAEGKGVFLRDVDGRDYIDGVSSLWTNVHGHRKEQIDRAIREQL
NRVAHSTLLGLAHEPAIVCARKLLEIVPKGLTRVFYSESGSTAVEIALKMAFQYQQQAAGGDPRKIRFMSLTNAYHGDTL
GSVSAGGIDLFHATYRELLFPTVKAAAPYCYRCPFGASYPGCRFDCLSQLENLLAAHAHELAALIIEPLVQGAAGILVQP
PGYLKRVRELCTRYGVLMIADEVAVGFGKTGRMFACEHEDVAPDILALGKGLSGGYLPLAATVATEEIYKGFLARFDEFK
TFFHGHTYTGNPLACAAAIANLDVFAEERVIEGSGEKIERLTQGLTPIASLNHVGEVRQRGFMVGIELVADRETKALYPP
AERIGHKVILEARKRGLIIRPLGDVIVLMPPLCITTEEIDRLCEITYESIRAVAGE

Sequences:

>Translated_456_residues
MNGQEEKKRQLVADGLKSIWFPFTQMQEFTPDDVLIIAEGKGVFLRDVDGRDYIDGVSSLWTNVHGHRKEQIDRAIREQL
NRVAHSTLLGLAHEPAIVCARKLLEIVPKGLTRVFYSESGSTAVEIALKMAFQYQQQAAGGDPRKIRFMSLTNAYHGDTL
GSVSAGGIDLFHATYRELLFPTVKAAAPYCYRCPFGASYPGCRFDCLSQLENLLAAHAHELAALIIEPLVQGAAGILVQP
PGYLKRVRELCTRYGVLMIADEVAVGFGKTGRMFACEHEDVAPDILALGKGLSGGYLPLAATVATEEIYKGFLARFDEFK
TFFHGHTYTGNPLACAAAIANLDVFAEERVIEGSGEKIERLTQGLTPIASLNHVGEVRQRGFMVGIELVADRETKALYPP
AERIGHKVILEARKRGLIIRPLGDVIVLMPPLCITTEEIDRLCEITYESIRAVAGE
>Mature_456_residues
MNGQEEKKRQLVADGLKSIWFPFTQMQEFTPDDVLIIAEGKGVFLRDVDGRDYIDGVSSLWTNVHGHRKEQIDRAIREQL
NRVAHSTLLGLAHEPAIVCARKLLEIVPKGLTRVFYSESGSTAVEIALKMAFQYQQQAAGGDPRKIRFMSLTNAYHGDTL
GSVSAGGIDLFHATYRELLFPTVKAAAPYCYRCPFGASYPGCRFDCLSQLENLLAAHAHELAALIIEPLVQGAAGILVQP
PGYLKRVRELCTRYGVLMIADEVAVGFGKTGRMFACEHEDVAPDILALGKGLSGGYLPLAATVATEEIYKGFLARFDEFK
TFFHGHTYTGNPLACAAAIANLDVFAEERVIEGSGEKIERLTQGLTPIASLNHVGEVRQRGFMVGIELVADRETKALYPP
AERIGHKVILEARKRGLIIRPLGDVIVLMPPLCITTEEIDRLCEITYESIRAVAGE

Specific function: Catalyzes the formation of the intermediate 7,8- diaminopelargonic acid (DAPA) from 7-keto-8-aminopelargonic acid (KAPA) with lysine as the amino donor. B.subtilis does not utilize S-adenosylmethionine (SAM), but instead utilizes only L-lysine as the amin

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=424, Percent_Identity=32.311320754717, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI13994255, Length=447, Percent_Identity=30.8724832214765, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI284507298, Length=337, Percent_Identity=34.1246290801187, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI37574042, Length=443, Percent_Identity=25.7336343115124, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI226442705, Length=442, Percent_Identity=26.2443438914027, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI24119277, Length=425, Percent_Identity=26.1176470588235, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI226442709, Length=378, Percent_Identity=26.7195767195767, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1786991, Length=429, Percent_Identity=39.1608391608392, Blast_Score=271, Evalue=6e-74,
Organism=Escherichia coli, GI145693181, Length=415, Percent_Identity=35.6626506024096, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1789016, Length=413, Percent_Identity=30.0242130750605, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1788044, Length=410, Percent_Identity=30.9756097560976, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1789759, Length=445, Percent_Identity=28.7640449438202, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787560, Length=414, Percent_Identity=30.1932367149758, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786349, Length=376, Percent_Identity=29.7872340425532, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32564660, Length=447, Percent_Identity=31.3199105145414, Blast_Score=181, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI25144271, Length=427, Percent_Identity=28.1030444964871, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI25144274, Length=266, Percent_Identity=34.5864661654135, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI71992977, Length=441, Percent_Identity=26.530612244898, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6324386, Length=475, Percent_Identity=29.0526315789474, Blast_Score=199, Evalue=6e-52,
Organism=Saccharomyces cerevisiae, GI6324432, Length=437, Percent_Identity=28.1464530892449, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6323470, Length=424, Percent_Identity=26.8867924528302, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6321456, Length=290, Percent_Identity=27.2413793103448, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21357415, Length=426, Percent_Identity=31.924882629108, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI161085790, Length=453, Percent_Identity=30.242825607064, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI28574759, Length=437, Percent_Identity=30.8924485125858, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21356575, Length=457, Percent_Identity=29.9781181619256, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI281366494, Length=290, Percent_Identity=26.2068965517241, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24667139, Length=290, Percent_Identity=26.2068965517241, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24667143, Length=290, Percent_Identity=26.2068965517241, Blast_Score=86, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.62

Molecular weight: Translated: 50130; Mature: 50130

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGQEEKKRQLVADGLKSIWFPFTQMQEFTPDDVLIIAEGKGVFLRDVDGRDYIDGVSSL
CCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECCCCEEEEECCCCHHHHHHHHH
WTNVHGHRKEQIDRAIREQLNRVAHSTLLGLAHEPAIVCARKLLEIVPKGLTRVFYSESG
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
STAVEIALKMAFQYQQQAAGGDPRKIRFMSLTNAYHGDTLGSVSAGGIDLFHATYRELLF
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
PTVKAAAPYCYRCPFGASYPGCRFDCLSQLENLLAAHAHELAALIIEPLVQGAAGILVQP
HHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
PGYLKRVRELCTRYGVLMIADEVAVGFGKTGRMFACEHEDVAPDILALGKGLSGGYLPLA
CHHHHHHHHHHHHCCEEEEEHHHHHCCCCCCCEEEECCCCCCHHHHHHCCCCCCCCHHHH
ATVATEEIYKGFLARFDEFKTFFHGHTYTGNPLACAAAIANLDVFAEERVIEGSGEKIER
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LTQGLTPIASLNHVGEVRQRGFMVGIELVADRETKALYPPAERIGHKVILEARKRGLIIR
HHHCCCHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCEEEE
PLGDVIVLMPPLCITTEEIDRLCEITYESIRAVAGE
ECCCCHHHCCCCEECHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNGQEEKKRQLVADGLKSIWFPFTQMQEFTPDDVLIIAEGKGVFLRDVDGRDYIDGVSSL
CCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECCCCEEEEECCCCHHHHHHHHH
WTNVHGHRKEQIDRAIREQLNRVAHSTLLGLAHEPAIVCARKLLEIVPKGLTRVFYSESG
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
STAVEIALKMAFQYQQQAAGGDPRKIRFMSLTNAYHGDTLGSVSAGGIDLFHATYRELLF
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
PTVKAAAPYCYRCPFGASYPGCRFDCLSQLENLLAAHAHELAALIIEPLVQGAAGILVQP
HHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
PGYLKRVRELCTRYGVLMIADEVAVGFGKTGRMFACEHEDVAPDILALGKGLSGGYLPLA
CHHHHHHHHHHHHCCEEEEEHHHHHCCCCCCCEEEECCCCCCHHHHHHCCCCCCCCHHHH
ATVATEEIYKGFLARFDEFKTFFHGHTYTGNPLACAAAIANLDVFAEERVIEGSGEKIER
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LTQGLTPIASLNHVGEVRQRGFMVGIELVADRETKALYPPAERIGHKVILEARKRGLIIR
HHHCCCHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCEEEE
PLGDVIVLMPPLCITTEEIDRLCEITYESIRAVAGE
ECCCCHHHCCCCEECHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; 8-amino-7-oxononanoate

Specific reaction: S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8763940; 9387221; 9384377 [H]