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The map label for this gene is gltX1

Identifier: 85858037

GI number: 85858037

Start: 200427

End: 201845

Strand: Direct

Name: gltX1

Synonym: SYN_02557

Alternate gene names: 85858037

Gene position: 200427-201845 (Clockwise)

Preceding gene: 85858022

Following gene: 85858038

Centisome position: 6.3

GC content: 55.67

Gene sequence:

>1419_bases
ATGCAGAAATTAAAAGAGATTCGCACCCGTTTTGCCCCTTCGCCGACCGGTTATCTTCACATCGGCGGCGCCCGCACGGC
CCTGTTCAGCTGGCTCTATGCCCGGCATCATCAGGGAAAGTTCGTTTTAAGAATTGAAGACACGGATCAGCTCCGTTCCA
CGGAAGAATCAACGCGGGCGATCCTGGATGCCATGACCTGGCTGGGATTGAACTGGGATGAAGGCCCCGTTTTCCAGGCG
GAACGCGTCGATATCCACCGGGCCATGATTCGGAAGCTGGTTGATGAGGATAAGGCCTATTACTGCACCTGCACCCCGGA
TGAGCTGGAAGAAAAACGGAAAAGAGCCCTGGCGGAAGGCCGCAAGCCCAAATACGACGGAACCTGCCGTGAGAAGAAAC
TCCCTCCCTCCCCCGGAACGGTGGTGCGGTTCCGTTGCCCGCAAACAGGAATCACGGTCGTCGATGACCTGATCAAGGGG
AAAATCTCCTTTAACAACGAAGAACTTGACGATCTGATCATTCAGCGCAGCGACGGCTATCCGACTTACAATTTCGCCGT
GGTCGTCGATGACGCCCAGATGGGAATATCCCATGTGATCCGGGGCGACGACCATGTCAACAACACTCCCCGGCAGATCC
TTCTCTACCAGGCCCTGGGGTACGATATTCCGCACTTCGGCCATGTGCCTATGATCCTGGGCGCCGACAAGGCCCGCCTC
AGCAAGCGTCATGGAGCAACCTCCGTAATGGCTTATAAGGACATGGGATACCTTCCGGAGGCCCTGGTGAACTATCTGGT
CCGCCTGGGCTGGTCGCACGGGGATCAGGAAATCTTTTCCCTGGACGAACTCATCGCCCTGTTTGGACTGGAAAGTATAG
GCAAATCCGCAGCCGTCTTCAATCCGGAGAAGCTGCTCTGGTTGAATCAGCATTACATCAAAACCTATCCGGAAGACAGA
CTGCTGGAGGTTCTGCAGCCTTTCTGGAAGCAACTGGGCATTGAAGCTCCTGATCCTGACTATGGCCGGAGCATCGTTCG
GGATCTCCGGGCCAGGGCCAAGACGCTGGTCGACATGGCGGAGTCAAGCACCTTTTATTTCAACGATGAGCCGGCCATTG
ATGCTGATGCCGCAAAAAAATTTCTCACTCCGGAAATCGCCGGTCATCTCGAAGCCATCGCGGAAGCACTGGCGACTCTT
GGGGATTACTCCAAAGAAGGAATTGAAATCTTTCTCCGCAGCCTGGTCGAGGCGAGAGCCATCAAACTCAAGACGATTGC
CCAGCCGCTCCGCATCGCTCTGACCGGAAAAACCGTCAGTCCCGGTCTGGACGACATCATGCTGACCCTGGGAAAGGAGC
GGGTGATTGCGCGCATTCAGCGCACGGTAGCGTATATCAGAAGTGGAATGGCATCCTGA

Upstream 100 bases:

>100_bases
ATGCTGTTGTCAAGCACAATTTGCTTGAAAACCGTCTTGACAAACCAGAAAAGCTGGACCTATAAAGGGGCACGATTAAA
GTCTGTCAGGAAAGGTTATA

Downstream 100 bases:

>100_bases
AATCTTTTGCCTTGACTTTTTCGTCTCAGTTGAATAAATAACTCCCCTTCAAGTGGTCCCATCGTCTAGTGGTTAGGACG
CTGGCCTCTCACGCCGGAAG

Product: glutamyl glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MQKLKEIRTRFAPSPTGYLHIGGARTALFSWLYARHHQGKFVLRIEDTDQLRSTEESTRAILDAMTWLGLNWDEGPVFQA
ERVDIHRAMIRKLVDEDKAYYCTCTPDELEEKRKRALAEGRKPKYDGTCREKKLPPSPGTVVRFRCPQTGITVVDDLIKG
KISFNNEELDDLIIQRSDGYPTYNFAVVVDDAQMGISHVIRGDDHVNNTPRQILLYQALGYDIPHFGHVPMILGADKARL
SKRHGATSVMAYKDMGYLPEALVNYLVRLGWSHGDQEIFSLDELIALFGLESIGKSAAVFNPEKLLWLNQHYIKTYPEDR
LLEVLQPFWKQLGIEAPDPDYGRSIVRDLRARAKTLVDMAESSTFYFNDEPAIDADAAKKFLTPEIAGHLEAIAEALATL
GDYSKEGIEIFLRSLVEARAIKLKTIAQPLRIALTGKTVSPGLDDIMLTLGKERVIARIQRTVAYIRSGMAS

Sequences:

>Translated_472_residues
MQKLKEIRTRFAPSPTGYLHIGGARTALFSWLYARHHQGKFVLRIEDTDQLRSTEESTRAILDAMTWLGLNWDEGPVFQA
ERVDIHRAMIRKLVDEDKAYYCTCTPDELEEKRKRALAEGRKPKYDGTCREKKLPPSPGTVVRFRCPQTGITVVDDLIKG
KISFNNEELDDLIIQRSDGYPTYNFAVVVDDAQMGISHVIRGDDHVNNTPRQILLYQALGYDIPHFGHVPMILGADKARL
SKRHGATSVMAYKDMGYLPEALVNYLVRLGWSHGDQEIFSLDELIALFGLESIGKSAAVFNPEKLLWLNQHYIKTYPEDR
LLEVLQPFWKQLGIEAPDPDYGRSIVRDLRARAKTLVDMAESSTFYFNDEPAIDADAAKKFLTPEIAGHLEAIAEALATL
GDYSKEGIEIFLRSLVEARAIKLKTIAQPLRIALTGKTVSPGLDDIMLTLGKERVIARIQRTVAYIRSGMAS
>Mature_472_residues
MQKLKEIRTRFAPSPTGYLHIGGARTALFSWLYARHHQGKFVLRIEDTDQLRSTEESTRAILDAMTWLGLNWDEGPVFQA
ERVDIHRAMIRKLVDEDKAYYCTCTPDELEEKRKRALAEGRKPKYDGTCREKKLPPSPGTVVRFRCPQTGITVVDDLIKG
KISFNNEELDDLIIQRSDGYPTYNFAVVVDDAQMGISHVIRGDDHVNNTPRQILLYQALGYDIPHFGHVPMILGADKARL
SKRHGATSVMAYKDMGYLPEALVNYLVRLGWSHGDQEIFSLDELIALFGLESIGKSAAVFNPEKLLWLNQHYIKTYPEDR
LLEVLQPFWKQLGIEAPDPDYGRSIVRDLRARAKTLVDMAESSTFYFNDEPAIDADAAKKFLTPEIAGHLEAIAEALATL
GDYSKEGIEIFLRSLVEARAIKLKTIAQPLRIALTGKTVSPGLDDIMLTLGKERVIARIQRTVAYIRSGMAS

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=496, Percent_Identity=33.0645161290323, Blast_Score=254, Evalue=1e-67,
Organism=Escherichia coli, GI1788743, Length=464, Percent_Identity=50.8620689655172, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI226510925, Length=242, Percent_Identity=38.8429752066116, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1786895, Length=348, Percent_Identity=22.7011494252874, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71993394, Length=483, Percent_Identity=29.6066252587992, Blast_Score=206, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6324540, Length=500, Percent_Identity=32.6, Blast_Score=257, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6321192, Length=237, Percent_Identity=29.535864978903, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6324742, Length=214, Percent_Identity=27.5700934579439, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24665321, Length=488, Percent_Identity=34.2213114754098, Blast_Score=274, Evalue=1e-73,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_SYNAS (Q2LQN4)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_460239.1
- ProteinModelPortal:   Q2LQN4
- SMR:   Q2LQN4
- STRING:   Q2LQN4
- GeneID:   3884491
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_02557
- NMPDR:   fig|56780.10.peg.190
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- PhylomeDB:   Q2LQN4
- BioCyc:   SACI56780:SYN_02557-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 53249; Mature: 53249

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 242-242

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLKEIRTRFAPSPTGYLHIGGARTALFSWLYARHHQGKFVLRIEDTDQLRSTEESTRA
CCHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHH
ILDAMTWLGLNWDEGPVFQAERVDIHRAMIRKLVDEDKAYYCTCTPDELEEKRKRALAEG
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCC
RKPKYDGTCREKKLPPSPGTVVRFRCPQTGITVVDDLIKGKISFNNEELDDLIIQRSDGY
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCC
PTYNFAVVVDDAQMGISHVIRGDDHVNNTPRQILLYQALGYDIPHFGHVPMILGADKARL
CCEEEEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEECCCHHHH
SKRHGATSVMAYKDMGYLPEALVNYLVRLGWSHGDQEIFSLDELIALFGLESIGKSAAVF
HHHCCCCCEEEHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEE
NPEKLLWLNQHYIKTYPEDRLLEVLQPFWKQLGIEAPDPDYGRSIVRDLRARAKTLVDMA
CCHHEEEECHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ESSTFYFNDEPAIDADAAKKFLTPEIAGHLEAIAEALATLGDYSKEGIEIFLRSLVEARA
CCCEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
IKLKTIAQPLRIALTGKTVSPGLDDIMLTLGKERVIARIQRTVAYIRSGMAS
HHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQKLKEIRTRFAPSPTGYLHIGGARTALFSWLYARHHQGKFVLRIEDTDQLRSTEESTRA
CCHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHH
ILDAMTWLGLNWDEGPVFQAERVDIHRAMIRKLVDEDKAYYCTCTPDELEEKRKRALAEG
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCC
RKPKYDGTCREKKLPPSPGTVVRFRCPQTGITVVDDLIKGKISFNNEELDDLIIQRSDGY
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCC
PTYNFAVVVDDAQMGISHVIRGDDHVNNTPRQILLYQALGYDIPHFGHVPMILGADKARL
CCEEEEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEECCCHHHH
SKRHGATSVMAYKDMGYLPEALVNYLVRLGWSHGDQEIFSLDELIALFGLESIGKSAAVF
HHHCCCCCEEEHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEE
NPEKLLWLNQHYIKTYPEDRLLEVLQPFWKQLGIEAPDPDYGRSIVRDLRARAKTLVDMA
CCHHEEEECHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ESSTFYFNDEPAIDADAAKKFLTPEIAGHLEAIAEALATLGDYSKEGIEIFLRSLVEARA
CCCEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
IKLKTIAQPLRIALTGKTVSPGLDDIMLTLGKERVIARIQRTVAYIRSGMAS
HHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA