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The map label for this gene is otsA [H]

Identifier: 85857979

GI number: 85857979

Start: 137822

End: 140068

Strand: Direct

Name: otsA [H]

Synonym: SYN_00622

Alternate gene names: 85857979

Gene position: 137822-140068 (Clockwise)

Preceding gene: 85857978

Following gene: 85857980

Centisome position: 4.33

GC content: 59.86

Gene sequence:

>2247_bases
ATGACCAGCCGTTCACGCCGGGCCGATACAGCCCTCTGCTGGTGGACTGAACCCATGAAATGGCTGTTCCCACTGGACGT
CCAGCTTTCCGGGAGAGGCGACAAGTTCCGGGGCGGGCTCATTGTCGCTTCCAACCGGGAACCGTACGCACACCGAATGA
CCGATCAGGGATTGCAACTGGAATTCTCTGTTGGCGGGGTCGTTTCAGCCCTCGATACGGTCCTGCGGGTGACAGGGGGA
ACATGGGTTGCCTGGGGAAGCGGCAGCGGCGACCGGGAGGGGGCGGATGCCGGTGGCCGTCTGTGGGTCCCGCCGGAAAA
CCCGGCCTATACCCTCAGAAGAATCTGGCTTTCCGCGGCAGCGGTGGAAAATTATTATCACGGGTTCTGCAATCTGGTCC
TCTGGCCGCTCTTTCATGACGAAACGGATCGTATCGTCTACGCTCCCCCTTTCTGGGCGGAGTATGAAAAGACCAACCGG
GTGTTTGCCGAAGCGATTCTCGAAGAATGCAGGGCGGATTCCACGATCTGGATCCATGACTATCACCTCTGCCTCGTTCC
CCGGATGCTACGCGGGGTGTCCCCGGATCGGACCATCGCCCATTTCTGGCACATACCCTGGCCCGAGCGGGAACTGTTCG
CCTGTGCCCCCCATGGCGAAGAAATCCTTGCCGGGCTTCTCGGAAATGACCTTCTCGGCTTTCAGATTCCTCTCTATGCA
GGGAATTTCATGGCCTGTGCCGCGGCATGTCTCGGTGCGGGCATCGATTACGATGCCATGACCGTTGCCTGGGAGGGGCG
CATCACCCGGATTCGGCGCCTCAGGGAAAAATACCGTCTGCCCCGGCGTGTCGGCCTTGCGGTCGACCGGCTCGACTACA
CCAAGGGAATCGTTCAGCGACTCCGGGCGCTTGAGCTTTTTTTCCGAGACAACCCTGACTTCCGGGGGAAATTTTCCTTC
ATTCAGGTTGCCGTCATGACTCGGAACGGGGAGCCGTACCTGCGCCACCGCCGGGAAGTCGAGAACCGAATCGCCCGAAT
CAACGCGGAGTTCGGCACGGCGGACTGGAGACCGATCATCTATTTCAAAAACAAACTAGATCAGCAGGATCTTGTTGCCT
GTTACCGGATGGCGGATGTGGCCGTCATCACCCCGCTTCGTGACGGGATGAACCTGGTGGCGAAGGAATATGTGGCCTCC
AGGGGAGACGGGGACGGCGTCCTGATCCTGGGCAGACAGGCCGGCGCAGCCGCCGAACTGACCGAGTCGATTCTTGTCGA
TCCAACTGATATCGAGGCATGCGCCGCCGCCATCCATGAGGCTCTCACTCTGTCCAGCGGGGAGAAAAAAAAGCGGATGA
CGCAGTTAAGGGAACAGGTGCAGGGGAATACGGTTTATTCCTGGGTCGGCGACATCCTCGATGAGCTTTCCCTCCTCCCC
GTCACGAAACAGGGAGGAAAACATGCCCTCGCGCATGAGGATGAAATCGCCGCCCGCCTTGCCGGGCGGGAACTCTTTCT
CTGCCTGGATTTCGACGGCACTCTCGCCCCCATCGTGGAGCAGCCGGAACTGGCGGCCATACCGGACGATATCCGCCTCC
TCCTCGCCGTGCTGCAGGAGCATTATCCTGTCGCAGTGATCAGCGGCAGAAGCCTTGACGACATCAGGAATCGTGTGGGT
TTGCCGGGCCTTGTGTATGCGGGGAGCCATGGGGCGGAGACGGAAAAGGGAACGGGCGGCGACGGCGGCCGCGCCGCACT
GGATGCATTTCTCGCCGCGGTGCACCAGGCATTGGCCTGCCTTCCCGGGGTTCAGATAGAGGACAAGGGATTGACGGTCA
GCATCCATTTCAGGCGGATAGCGCCGGTGCTTCTGGAGAATTTCCTGGATTCTTTCCAGGGGATTGCCCGGAAGTTTGTG
GGAACGGTCAGTGTGATCGAAGGGAGAAAGGTCTTCGAGATCCGGCCGCAGGGGGCAACCGGCAAAGGGGACGCAGTGCG
GCAGCTACTGGAGGGAATAGGTAAAGGAGGGCTTCCGGTCTATATGGGAGATGACACGAACGATGAAGAAGCGTTCCGGG
CGGTTCGGGGGAATGGGATAACCGTCGCCGTCGGGGGAAGCACGGAGGCGGAATTCTATTTGAGGAACCAGGGGGAGGTC
AGGAAATTCCTCGCGTTGCTGGCCCGGATCTCACTTCCCGATGAAAAACAATGCTACGCAGTGGACGAAGGGGGTACAAC
AAAATGA

Upstream 100 bases:

>100_bases
GCCGCCGGCGGGCTCAGCCTCGTCCGGGGAAAGGAGCTCATGTATCTGGTGCTCAACTTCCTGGATCGGGGAAAACTCTG
GGGGCTCATGGATTCGCCCG

Downstream 100 bases:

>100_bases
TCGGCGAGACGGTTCCGAAGATCGAAACAGGGGTCATCCATGGCCGCTTTCAGGTGTTTCATAACGACCACCTAAAGTAC
CTTCTCGCCGGAATGCGACT

Product: bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB

Products: UDP; alpha,alpha'-Trehalose 6-phosphate

Alternate protein names: TPS; Trehalose-6-phosphate synthase [H]

Number of amino acids: Translated: 748; Mature: 747

Protein sequence:

>748_residues
MTSRSRRADTALCWWTEPMKWLFPLDVQLSGRGDKFRGGLIVASNREPYAHRMTDQGLQLEFSVGGVVSALDTVLRVTGG
TWVAWGSGSGDREGADAGGRLWVPPENPAYTLRRIWLSAAAVENYYHGFCNLVLWPLFHDETDRIVYAPPFWAEYEKTNR
VFAEAILEECRADSTIWIHDYHLCLVPRMLRGVSPDRTIAHFWHIPWPERELFACAPHGEEILAGLLGNDLLGFQIPLYA
GNFMACAAACLGAGIDYDAMTVAWEGRITRIRRLREKYRLPRRVGLAVDRLDYTKGIVQRLRALELFFRDNPDFRGKFSF
IQVAVMTRNGEPYLRHRREVENRIARINAEFGTADWRPIIYFKNKLDQQDLVACYRMADVAVITPLRDGMNLVAKEYVAS
RGDGDGVLILGRQAGAAAELTESILVDPTDIEACAAAIHEALTLSSGEKKKRMTQLREQVQGNTVYSWVGDILDELSLLP
VTKQGGKHALAHEDEIAARLAGRELFLCLDFDGTLAPIVEQPELAAIPDDIRLLLAVLQEHYPVAVISGRSLDDIRNRVG
LPGLVYAGSHGAETEKGTGGDGGRAALDAFLAAVHQALACLPGVQIEDKGLTVSIHFRRIAPVLLENFLDSFQGIARKFV
GTVSVIEGRKVFEIRPQGATGKGDAVRQLLEGIGKGGLPVYMGDDTNDEEAFRAVRGNGITVAVGGSTEAEFYLRNQGEV
RKFLALLARISLPDEKQCYAVDEGGTTK

Sequences:

>Translated_748_residues
MTSRSRRADTALCWWTEPMKWLFPLDVQLSGRGDKFRGGLIVASNREPYAHRMTDQGLQLEFSVGGVVSALDTVLRVTGG
TWVAWGSGSGDREGADAGGRLWVPPENPAYTLRRIWLSAAAVENYYHGFCNLVLWPLFHDETDRIVYAPPFWAEYEKTNR
VFAEAILEECRADSTIWIHDYHLCLVPRMLRGVSPDRTIAHFWHIPWPERELFACAPHGEEILAGLLGNDLLGFQIPLYA
GNFMACAAACLGAGIDYDAMTVAWEGRITRIRRLREKYRLPRRVGLAVDRLDYTKGIVQRLRALELFFRDNPDFRGKFSF
IQVAVMTRNGEPYLRHRREVENRIARINAEFGTADWRPIIYFKNKLDQQDLVACYRMADVAVITPLRDGMNLVAKEYVAS
RGDGDGVLILGRQAGAAAELTESILVDPTDIEACAAAIHEALTLSSGEKKKRMTQLREQVQGNTVYSWVGDILDELSLLP
VTKQGGKHALAHEDEIAARLAGRELFLCLDFDGTLAPIVEQPELAAIPDDIRLLLAVLQEHYPVAVISGRSLDDIRNRVG
LPGLVYAGSHGAETEKGTGGDGGRAALDAFLAAVHQALACLPGVQIEDKGLTVSIHFRRIAPVLLENFLDSFQGIARKFV
GTVSVIEGRKVFEIRPQGATGKGDAVRQLLEGIGKGGLPVYMGDDTNDEEAFRAVRGNGITVAVGGSTEAEFYLRNQGEV
RKFLALLARISLPDEKQCYAVDEGGTTK
>Mature_747_residues
TSRSRRADTALCWWTEPMKWLFPLDVQLSGRGDKFRGGLIVASNREPYAHRMTDQGLQLEFSVGGVVSALDTVLRVTGGT
WVAWGSGSGDREGADAGGRLWVPPENPAYTLRRIWLSAAAVENYYHGFCNLVLWPLFHDETDRIVYAPPFWAEYEKTNRV
FAEAILEECRADSTIWIHDYHLCLVPRMLRGVSPDRTIAHFWHIPWPERELFACAPHGEEILAGLLGNDLLGFQIPLYAG
NFMACAAACLGAGIDYDAMTVAWEGRITRIRRLREKYRLPRRVGLAVDRLDYTKGIVQRLRALELFFRDNPDFRGKFSFI
QVAVMTRNGEPYLRHRREVENRIARINAEFGTADWRPIIYFKNKLDQQDLVACYRMADVAVITPLRDGMNLVAKEYVASR
GDGDGVLILGRQAGAAAELTESILVDPTDIEACAAAIHEALTLSSGEKKKRMTQLREQVQGNTVYSWVGDILDELSLLPV
TKQGGKHALAHEDEIAARLAGRELFLCLDFDGTLAPIVEQPELAAIPDDIRLLLAVLQEHYPVAVISGRSLDDIRNRVGL
PGLVYAGSHGAETEKGTGGDGGRAALDAFLAAVHQALACLPGVQIEDKGLTVSIHFRRIAPVLLENFLDSFQGIARKFVG
TVSVIEGRKVFEIRPQGATGKGDAVRQLLEGIGKGGLPVYMGDDTNDEEAFRAVRGNGITVAVGGSTEAEFYLRNQGEVR
KFLALLARISLPDEKQCYAVDEGGTTK

Specific function: Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6- phosphate and nucleoside diphosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=470, Percent_Identity=31.063829787234, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1788207, Length=228, Percent_Identity=31.5789473684211, Blast_Score=87, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI115533558, Length=364, Percent_Identity=28.2967032967033, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI115533560, Length=364, Percent_Identity=28.2967032967033, Blast_Score=133, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI71987012, Length=424, Percent_Identity=26.6509433962264, Blast_Score=129, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6319602, Length=471, Percent_Identity=32.6963906581741, Blast_Score=248, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6320279, Length=686, Percent_Identity=26.6763848396501, Blast_Score=214, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6323537, Length=383, Percent_Identity=27.154046997389, Blast_Score=177, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6323917, Length=380, Percent_Identity=28.1578947368421, Blast_Score=171, Evalue=4e-43,
Organism=Drosophila melanogaster, GI19920676, Length=765, Percent_Identity=32.6797385620915, Blast_Score=339, Evalue=5e-93,
Organism=Drosophila melanogaster, GI20129309, Length=232, Percent_Identity=29.3103448275862, Blast_Score=93, Evalue=7e-19,
Organism=Drosophila melanogaster, GI221473368, Length=241, Percent_Identity=28.6307053941909, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI20129307, Length=241, Percent_Identity=28.6307053941909, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24582490, Length=241, Percent_Identity=28.6307053941909, Blast_Score=90, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: 2.4.1.15

Molecular weight: Translated: 82774; Mature: 82642

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRSRRADTALCWWTEPMKWLFPLDVQLSGRGDKFRGGLIVASNREPYAHRMTDQGLQL
CCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCCCCCCEEEECCCCCHHHHHCCCCCEE
EFSVGGVVSALDTVLRVTGGTWVAWGSGSGDREGADAGGRLWVPPENPAYTLRRIWLSAA
EEECCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHH
AVENYYHGFCNLVLWPLFHDETDRIVYAPPFWAEYEKTNRVFAEAILEECRADSTIWIHD
HHHHHHHHHHHHHEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEE
YHLCLVPRMLRGVSPDRTIAHFWHIPWPERELFACAPHGEEILAGLLGNDLLGFQIPLYA
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEECCCHHHHHHHHHCCCCCCEEEEHHC
GNFMACAAACLGAGIDYDAMTVAWEGRITRIRRLREKYRLPRRVGLAVDRLDYTKGIVQR
CCHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHCHHHHHHHHHHHHHHH
LRALELFFRDNPDFRGKFSFIQVAVMTRNGEPYLRHRREVENRIARINAEFGTADWRPII
HHHHHHHHCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHEEECCCCCCCCEEEE
YFKNKLDQQDLVACYRMADVAVITPLRDGMNLVAKEYVASRGDGDGVLILGRQAGAAAEL
EECCCCCHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHH
TESILVDPTDIEACAAAIHEALTLSSGEKKKRMTQLREQVQGNTVYSWVGDILDELSLLP
HHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
VTKQGGKHALAHEDEIAARLAGRELFLCLDFDGTLAPIVEQPELAAIPDDIRLLLAVLQE
EECCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
HYPVAVISGRSLDDIRNRVGLPGLVYAGSHGAETEKGTGGDGGRAALDAFLAAVHQALAC
CCCEEEEECCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LPGVQIEDKGLTVSIHFRRIAPVLLENFLDSFQGIARKFVGTVSVIEGRKVFEIRPQGAT
CCCCEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEECCCCCC
GKGDAVRQLLEGIGKGGLPVYMGDDTNDEEAFRAVRGNGITVAVGGSTEAEFYLRNQGEV
CCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHCCCCEEEEECCCCCEEEEECCCCHH
RKFLALLARISLPDEKQCYAVDEGGTTK
HHHHHHHHHCCCCCCCCEEEECCCCCCC
>Mature Secondary Structure 
TSRSRRADTALCWWTEPMKWLFPLDVQLSGRGDKFRGGLIVASNREPYAHRMTDQGLQL
CCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCCCCCCEEEECCCCCHHHHHCCCCCEE
EFSVGGVVSALDTVLRVTGGTWVAWGSGSGDREGADAGGRLWVPPENPAYTLRRIWLSAA
EEECCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHH
AVENYYHGFCNLVLWPLFHDETDRIVYAPPFWAEYEKTNRVFAEAILEECRADSTIWIHD
HHHHHHHHHHHHHEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEE
YHLCLVPRMLRGVSPDRTIAHFWHIPWPERELFACAPHGEEILAGLLGNDLLGFQIPLYA
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEECCCHHHHHHHHHCCCCCCEEEEHHC
GNFMACAAACLGAGIDYDAMTVAWEGRITRIRRLREKYRLPRRVGLAVDRLDYTKGIVQR
CCHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHCHHHHHHHHHHHHHHH
LRALELFFRDNPDFRGKFSFIQVAVMTRNGEPYLRHRREVENRIARINAEFGTADWRPII
HHHHHHHHCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHEEECCCCCCCCEEEE
YFKNKLDQQDLVACYRMADVAVITPLRDGMNLVAKEYVASRGDGDGVLILGRQAGAAAEL
EECCCCCHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHH
TESILVDPTDIEACAAAIHEALTLSSGEKKKRMTQLREQVQGNTVYSWVGDILDELSLLP
HHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
VTKQGGKHALAHEDEIAARLAGRELFLCLDFDGTLAPIVEQPELAAIPDDIRLLLAVLQE
EECCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
HYPVAVISGRSLDDIRNRVGLPGLVYAGSHGAETEKGTGGDGGRAALDAFLAAVHQALAC
CCCEEEEECCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LPGVQIEDKGLTVSIHFRRIAPVLLENFLDSFQGIARKFVGTVSVIEGRKVFEIRPQGAT
CCCCEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEECCCCCC
GKGDAVRQLLEGIGKGGLPVYMGDDTNDEEAFRAVRGNGITVAVGGSTEAEFYLRNQGEV
CCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHCCCCEEEEECCCCCEEEEECCCCHH
RKFLALLARISLPDEKQCYAVDEGGTTK
HHHHHHHHHCCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Monovalent cations [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose; D-glucose 6-phosphate

Specific reaction: UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate

General reaction: Hexosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA