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The map label for this gene is hycI [C]

Identifier: 85857972

GI number: 85857972

Start: 131385

End: 131870

Strand: Direct

Name: hycI [C]

Synonym: SYN_02223

Alternate gene names: 85857972

Gene position: 131385-131870 (Clockwise)

Preceding gene: 85857971

Following gene: 85857977

Centisome position: 4.13

GC content: 52.06

Gene sequence:

>486_bases
ATGTCAAATTTGTTGAATGAAGAAGTCCTGTCGGCCCTGAGGCCGGAGGGACGCACGGTGATGATTACGATCGGCAATTC
CCTGAGGGCCGACGATGGCGTCGGCCCTTTCATCGGGAGGAAAATTGCCGATCAGAAGGACTTCCTTGTCCTGAATGCCC
TGATGAATCCGGAAGATATCGTGGAGGATGCGATCGCTTATCGACCTGGAAAAATAGTCGTCATCGATGCCGCGGATTTC
GGTGGCTCACCGGGAGAAATACGAACCATTCCCCTTGATCAGATTCAGCGTTATACGGTTTTTTCCACCCACCATTTCCC
GATTTCCGTGCTCCTGGGCATCATTCGGGAAGAGACGAACGCCAGCATGGCGATTCTGGGCATCCAGCCGGAATCCGTAG
AATTCGGTGAATGCATGTGTTCCGAGGTCCGGCAGACCGCCCTGGATATCATTGAATATCTGAATAATCTCTGCAACTCG
TTATAG

Upstream 100 bases:

>100_bases
CCCGAGATTCTCGACCAGACAAAGGAAGAAATCACCCTGAAGATGGAAATGCTGGTTCGGGCTTACGATCCCTGCATTTC
CTGCTCCGCCCATTATCTGA

Downstream 100 bases:

>100_bases
CGTATCCCGAGAATCTTAAATTCCCTGTCTTTTCTTGATTTGACACATAGCCGCCCCAAAGGCTGTCGGTTTTTTTTATG
AAGAAATAATGACGCCGTCT

Product: Ni,Fe-hydrogenase maturation factor

Products: reduced coenzyme F420

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MSNLLNEEVLSALRPEGRTVMITIGNSLRADDGVGPFIGRKIADQKDFLVLNALMNPEDIVEDAIAYRPGKIVVIDAADF
GGSPGEIRTIPLDQIQRYTVFSTHHFPISVLLGIIREETNASMAILGIQPESVEFGECMCSEVRQTALDIIEYLNNLCNS
L

Sequences:

>Translated_161_residues
MSNLLNEEVLSALRPEGRTVMITIGNSLRADDGVGPFIGRKIADQKDFLVLNALMNPEDIVEDAIAYRPGKIVVIDAADF
GGSPGEIRTIPLDQIQRYTVFSTHHFPISVLLGIIREETNASMAILGIQPESVEFGECMCSEVRQTALDIIEYLNNLCNS
L
>Mature_160_residues
SNLLNEEVLSALRPEGRTVMITIGNSLRADDGVGPFIGRKIADQKDFLVLNALMNPEDIVEDAIAYRPGKIVVIDAADFG
GSPGEIRTIPLDQIQRYTVFSTHHFPISVLLGIIREETNASMAILGIQPESVEFGECMCSEVRQTALDIIEYLNNLCNSL

Specific function: Protease Involved In The C-Terminal Processing Of Hyce, The Large Subunit Of Hydrogenase 3. [C]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789072, Length=137, Percent_Identity=30.6569343065693, Blast_Score=77, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004420
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 1.12.98.1

Molecular weight: Translated: 17678; Mature: 17546

Theoretical pI: Translated: 4.22; Mature: 4.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLLNEEVLSALRPEGRTVMITIGNSLRADDGVGPFIGRKIADQKDFLVLNALMNPEDI
CCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCEEEEEHHCCHHHH
VEDAIAYRPGKIVVIDAADFGGSPGEIRTIPLDQIQRYTVFSTHHFPISVLLGIIREETN
HHHHHHCCCCCEEEEECCCCCCCCCCEEECCHHHHHHEEEEECCCCHHHHHHHHHHHCCC
ASMAILGIQPESVEFGECMCSEVRQTALDIIEYLNNLCNSL
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNLLNEEVLSALRPEGRTVMITIGNSLRADDGVGPFIGRKIADQKDFLVLNALMNPEDI
CCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCEEEEEHHCCHHHH
VEDAIAYRPGKIVVIDAADFGGSPGEIRTIPLDQIQRYTVFSTHHFPISVLLGIIREETN
HHHHHHCCCCCEEEEECCCCCCCCCCEEECCHHHHHHEEEEECCCCHHHHHHHHHHHCCC
ASMAILGIQPESVEFGECMCSEVRQTALDIIEYLNNLCNSL
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2; coenzyme F420

Specific reaction: H2 + coenzyme F420 = reduced coenzyme F420

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]