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The map label for this gene is fumA [H]

Identifier: 85857927

GI number: 85857927

Start: 83723

End: 84319

Strand: Direct

Name: fumA [H]

Synonym: SYN_00158

Alternate gene names: 85857927

Gene position: 83723-84319 (Clockwise)

Preceding gene: 85857926

Following gene: 85857928

Centisome position: 2.63

GC content: 59.97

Gene sequence:

>597_bases
GTGTCACGTGGCTCGGCACCGGGAGGCGGTGATTTAATGATGAGCGAACCGATCCGTCTGGAAACACCCTTAAATGAGGA
TCTGTGCAGGCGTCTGTCTGTGGGCGACCGGGTTCTTCTCAACGGAGTCGTCTATACCGGTCGGGATGCGGCCCATCGCC
GCCTTTGCGAGGCCGCCGGACGCGGCGAGCCTTTGCCGTTTCCGATTGAGGGAGCGGTGATCTTCTATGCCGGGCCGGCC
CCGGCAAGGCCGGGTTCCATAACCGGATCCATCGGTCCGACTACGAGTTGCCGGATGGATTCCTTTGCGCCGCAGTTGAT
GGCTCTGGGATTGAAAGGGATGATCGGCAAGGGGAAGCGTTCAGCGGAGGTCGTTGCCGCCATGAAGCGGTACAAGGCGG
TTTATTTTGGTGCCATCGGGGGAATTGCGGCACTGACGGCCCGCTGCATCCGGGAATCCTCTGTCATTGCCTATGAGGAT
CTCGGTCCAGAGGCCATCTTCCGATTGGTCGTCTTCGAATTGCCCCTGGTGGTGATCAACGACACCCGGGGGAGGGATCT
TTACGACGAAGCCTTGAAGGCCTATGCCCTGACTTGA

Upstream 100 bases:

>100_bases
TCGGGCCGCAGGGGTATGGGGGGCGTGTGACCGCCCTGGCCGTCCATGTGGAAATGATGCCCTGCCATATCGCCAGTCTG
CCCGTTACTGTCAACATCCA

Downstream 100 bases:

>100_bases
ATGGAGCAGGCGCGTGTATTGATGTTGAAAACGGTTGCATCGGGTAAAAATGACAATCAATGAGTTACAGTCTACATCAT
TATTATAATAAATGACTGTA

Product: fumarate hydratase subunit beta

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MSRGSAPGGGDLMMSEPIRLETPLNEDLCRRLSVGDRVLLNGVVYTGRDAAHRRLCEAAGRGEPLPFPIEGAVIFYAGPA
PARPGSITGSIGPTTSCRMDSFAPQLMALGLKGMIGKGKRSAEVVAAMKRYKAVYFGAIGGIAALTARCIRESSVIAYED
LGPEAIFRLVVFELPLVVINDTRGRDLYDEALKAYALT

Sequences:

>Translated_198_residues
MSRGSAPGGGDLMMSEPIRLETPLNEDLCRRLSVGDRVLLNGVVYTGRDAAHRRLCEAAGRGEPLPFPIEGAVIFYAGPA
PARPGSITGSIGPTTSCRMDSFAPQLMALGLKGMIGKGKRSAEVVAAMKRYKAVYFGAIGGIAALTARCIRESSVIAYED
LGPEAIFRLVVFELPLVVINDTRGRDLYDEALKAYALT
>Mature_197_residues
SRGSAPGGGDLMMSEPIRLETPLNEDLCRRLSVGDRVLLNGVVYTGRDAAHRRLCEAAGRGEPLPFPIEGAVIFYAGPAP
ARPGSITGSIGPTTSCRMDSFAPQLMALGLKGMIGKGKRSAEVVAAMKRYKAVYFGAIGGIAALTARCIRESSVIAYEDL
GPEAIFRLVVFELPLVVINDTRGRDLYDEALKAYALT

Specific function: It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically [H]

COG id: COG1838

COG function: function code C; Tartrate dehydratase beta subunit/Fumarate hydratase class I, C-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=177, Percent_Identity=39.5480225988701, Blast_Score=122, Evalue=1e-29,
Organism=Escherichia coli, GI1790564, Length=177, Percent_Identity=37.2881355932203, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1789443, Length=182, Percent_Identity=34.6153846153846, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 21105; Mature: 20974

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRGSAPGGGDLMMSEPIRLETPLNEDLCRRLSVGDRVLLNGVVYTGRDAAHRRLCEAAG
CCCCCCCCCCCEEECCCEEEECCCCHHHHHHHCCCCEEEECCEEEECCHHHHHHHHHHHC
RGEPLPFPIEGAVIFYAGPAPARPGSITGSIGPTTSCRMDSFAPQLMALGLKGMIGKGKR
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCH
SAEVVAAMKRYKAVYFGAIGGIAALTARCIRESSVIAYEDLGPEAIFRLVVFELPLVVIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCEEEEE
DTRGRDLYDEALKAYALT
CCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SRGSAPGGGDLMMSEPIRLETPLNEDLCRRLSVGDRVLLNGVVYTGRDAAHRRLCEAAG
CCCCCCCCCCEEECCCEEEECCCCHHHHHHHCCCCEEEECCEEEECCHHHHHHHHHHHC
RGEPLPFPIEGAVIFYAGPAPARPGSITGSIGPTTSCRMDSFAPQLMALGLKGMIGKGKR
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCH
SAEVVAAMKRYKAVYFGAIGGIAALTARCIRESSVIAYEDLGPEAIFRLVVFELPLVVIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCEEEEE
DTRGRDLYDEALKAYALT
CCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 1879695 [H]