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The map label for this gene is pyk [H]

Identifier: 85375724

GI number: 85375724

Start: 2918728

End: 2920191

Strand: Reverse

Name: pyk [H]

Synonym: ELI_14485

Alternate gene names: 85375724

Gene position: 2920191-2918728 (Counterclockwise)

Preceding gene: 85375729

Following gene: 85375717

Centisome position: 95.67

GC content: 64.69

Gene sequence:

>1464_bases
ATGTCCGATCATACCAAGATTGATCCACGGGGTCGCAAGGTCAAAATCCTCGCCACCGTCGGCCCCGCCAGCCGCGAACC
CGAGATGCTCCGCCGCCTGTTCAAGGCCGGTGCAGACGCCTTCCGCGTCAACATGAGCCATGGCGATCACGCCACCCATG
CCGAAACGATCAAGGCGATCCGCGAGCTGGAACGCGAATTCTATCGCCCGGTCGCGATCCTGTGCGACCTCCAGGGCCCC
AAACTGCGCGTCGGGAAATTCAGGGACGGCAAGGCGGTCATCCGTCACTCGGGTCATTTCACGCTCGATCGCAATCCCGA
GCCGGGCGACGAGACCCGCGTCGAGCTCCCGCATCCCGAGCTGTTCGGGCTTCTCAGCAAGGGCGACCGCCTGCTGATCA
ATGACGGCAAGATCCGGCTCAAGGTCAAGGAAGCCGATGACGAGCGGATATTCTGTTCGGCCGAAGTCGGCGGCGTGATC
TCGGACCGCAAGGGTGTCAACGTGCCGGATGCCGAAATCCCGATTCCGGCTTTGACCGAGAAGGATCGCAAGGATCTGGC
GTTTGCGATGGAGAACGGCGCGGACTGGATCGGGCTCAGCTTCGTCCAGCGGCCTGAAGACCTGGCCGAAGCGCGCAAGC
TGATGGGCGGCAAGGGCGCGCTGTGCGCCAAGATCGAAAAGCCGATGGCAGTGCGCCGGCTGGATGAGCTGATCGAGATG
TCCGACGGCATCATGGTCGCGCGCGGTGACTTGGGCGTCGAACTGGAGCCGCAGGAAGTGCCGCCGCTGCAGAAGAAGAT
CGTCAATGCCACCCGGCTCAAGGGCAAACCGGTAATCGTCGCAACGCAGATGCTGGAAAGCATGATCGAAAGCCCCGCCC
CGACGCGCGCGGAAGTCTCCGATGTCGCAAACGCGGTCTATGACGGGGCCGACGCGGTCATGCTGAGCGCGGAGACGGCT
GCGGGGGAATGGCCCGAGGAAGCGGTCACCATCATGCACAAGATCGCCCAGCAGGTGGAGCGCGATCCCGGCTATGTCGA
GCGCGTGCGGCTGCTCGATACACCGCCCGATCCGACCACCGCCGATGCGCTGGCGCATGCCTGCATGACCATCGCCGATA
CCGTGCCGATCAGCGCCGTCACGGTGTTCACCGGCAGCGGCTCGACCGCCCGCCGCGTCGCGCGCGAGCGTCCCTCGGTA
CCGATGCTGGTGCTGACCCCGCATATGCGCACGGCTCGGCGGTTAGGGCTGCTCTGGGGCGCTCACGCCGTGGCGACCAA
GGATATCGGCAGTTTCGAAGAGATGATCGCCAAAGGCAAACGCATGGCTTTGCGCCATGGCTTCGGTAAAGCGGGCAGCA
AGCTCATTGCCCTTGCCGGTGTACCGTTCGGGACCCCGGGCAGCACCAATCTGCTGCACGTCGTGACGCTGGCCGGCGAC
GAGCTGGAGAAGCACGGCGGATGA

Upstream 100 bases:

>100_bases
GGCCACTTCGTCGTCCAACGCATCGAGCGGGTCGGGTGCATTCGTATCCATTGAAACCATTTCCTTGAGTTGCAACACCG
CTGTGTCATAGCGTCAGTCC

Downstream 100 bases:

>100_bases
CGGCAGAGCCGCGCTGTCACTTCCCGCGCAGACTCTCGAATACTTAACCGCCGCGTTGATCGGCGGCGCTCTGCAACCGC
GCCCGCGCTTCTTCCTCTCC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSDHTKIDPRGRKVKILATVGPASREPEMLRRLFKAGADAFRVNMSHGDHATHAETIKAIRELEREFYRPVAILCDLQGP
KLRVGKFRDGKAVIRHSGHFTLDRNPEPGDETRVELPHPELFGLLSKGDRLLINDGKIRLKVKEADDERIFCSAEVGGVI
SDRKGVNVPDAEIPIPALTEKDRKDLAFAMENGADWIGLSFVQRPEDLAEARKLMGGKGALCAKIEKPMAVRRLDELIEM
SDGIMVARGDLGVELEPQEVPPLQKKIVNATRLKGKPVIVATQMLESMIESPAPTRAEVSDVANAVYDGADAVMLSAETA
AGEWPEEAVTIMHKIAQQVERDPGYVERVRLLDTPPDPTTADALAHACMTIADTVPISAVTVFTGSGSTARRVARERPSV
PMLVLTPHMRTARRLGLLWGAHAVATKDIGSFEEMIAKGKRMALRHGFGKAGSKLIALAGVPFGTPGSTNLLHVVTLAGD
ELEKHGG

Sequences:

>Translated_487_residues
MSDHTKIDPRGRKVKILATVGPASREPEMLRRLFKAGADAFRVNMSHGDHATHAETIKAIRELEREFYRPVAILCDLQGP
KLRVGKFRDGKAVIRHSGHFTLDRNPEPGDETRVELPHPELFGLLSKGDRLLINDGKIRLKVKEADDERIFCSAEVGGVI
SDRKGVNVPDAEIPIPALTEKDRKDLAFAMENGADWIGLSFVQRPEDLAEARKLMGGKGALCAKIEKPMAVRRLDELIEM
SDGIMVARGDLGVELEPQEVPPLQKKIVNATRLKGKPVIVATQMLESMIESPAPTRAEVSDVANAVYDGADAVMLSAETA
AGEWPEEAVTIMHKIAQQVERDPGYVERVRLLDTPPDPTTADALAHACMTIADTVPISAVTVFTGSGSTARRVARERPSV
PMLVLTPHMRTARRLGLLWGAHAVATKDIGSFEEMIAKGKRMALRHGFGKAGSKLIALAGVPFGTPGSTNLLHVVTLAGD
ELEKHGG
>Mature_486_residues
SDHTKIDPRGRKVKILATVGPASREPEMLRRLFKAGADAFRVNMSHGDHATHAETIKAIRELEREFYRPVAILCDLQGPK
LRVGKFRDGKAVIRHSGHFTLDRNPEPGDETRVELPHPELFGLLSKGDRLLINDGKIRLKVKEADDERIFCSAEVGGVIS
DRKGVNVPDAEIPIPALTEKDRKDLAFAMENGADWIGLSFVQRPEDLAEARKLMGGKGALCAKIEKPMAVRRLDELIEMS
DGIMVARGDLGVELEPQEVPPLQKKIVNATRLKGKPVIVATQMLESMIESPAPTRAEVSDVANAVYDGADAVMLSAETAA
GEWPEEAVTIMHKIAQQVERDPGYVERVRLLDTPPDPTTADALAHACMTIADTVPISAVTVFTGSGSTARRVARERPSVP
MLVLTPHMRTARRLGLLWGAHAVATKDIGSFEEMIAKGKRMALRHGFGKAGSKLIALAGVPFGTPGSTNLLHVVTLAGDE
LEKHGG

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=494, Percent_Identity=39.2712550607287, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI33286422, Length=489, Percent_Identity=39.2638036809816, Blast_Score=303, Evalue=3e-82,
Organism=Homo sapiens, GI33286420, Length=489, Percent_Identity=39.2638036809816, Blast_Score=303, Evalue=3e-82,
Organism=Homo sapiens, GI32967597, Length=495, Percent_Identity=37.1717171717172, Blast_Score=295, Evalue=4e-80,
Organism=Homo sapiens, GI10835121, Length=495, Percent_Identity=37.1717171717172, Blast_Score=295, Evalue=6e-80,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=39.0243902439024, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=39.0243902439024, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=36.2869198312236, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=36.2869198312236, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=34.8837209302326, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1787965, Length=473, Percent_Identity=35.9408033826639, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI1788160, Length=477, Percent_Identity=37.3165618448637, Blast_Score=280, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17544584, Length=485, Percent_Identity=38.1443298969072, Blast_Score=301, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI71984406, Length=489, Percent_Identity=38.241308793456, Blast_Score=270, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI71984413, Length=489, Percent_Identity=38.241308793456, Blast_Score=270, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI17506829, Length=489, Percent_Identity=38.241308793456, Blast_Score=270, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI17506831, Length=489, Percent_Identity=38.241308793456, Blast_Score=270, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6319279, Length=487, Percent_Identity=36.1396303901437, Blast_Score=270, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6324923, Length=486, Percent_Identity=34.9794238683128, Blast_Score=265, Evalue=1e-71,
Organism=Drosophila melanogaster, GI28571814, Length=494, Percent_Identity=37.0445344129555, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI24648964, Length=494, Percent_Identity=37.0445344129555, Blast_Score=285, Evalue=7e-77,
Organism=Drosophila melanogaster, GI24648966, Length=412, Percent_Identity=36.6504854368932, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24581235, Length=420, Percent_Identity=33.3333333333333, Blast_Score=236, Evalue=4e-62,
Organism=Drosophila melanogaster, GI24646914, Length=292, Percent_Identity=34.9315068493151, Blast_Score=154, Evalue=9e-38,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52911; Mature: 52780

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDHTKIDPRGRKVKILATVGPASREPEMLRRLFKAGADAFRVNMSHGDHATHAETIKAI
CCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHH
RELEREFYRPVAILCDLQGPKLRVGKFRDGKAVIRHSGHFTLDRNPEPGDETRVELPHPE
HHHHHHHCCCEEEEEECCCCCEEECCCCCCCEEEEECCEEEECCCCCCCCCCEEECCCHH
LFGLLSKGDRLLINDGKIRLKVKEADDERIFCSAEVGGVISDRKGVNVPDAEIPIPALTE
HHHHHCCCCEEEEECCEEEEEEEECCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCC
KDRKDLAFAMENGADWIGLSFVQRPEDLAEARKLMGGKGALCAKIEKPMAVRRLDELIEM
CCHHHHHHHHCCCCCEEEHHHHCCCHHHHHHHHHHCCCCCEEHHHCCCHHHHHHHHHHHH
SDGIMVARGDLGVELEPQEVPPLQKKIVNATRLKGKPVIVATQMLESMIESPAPTRAEVS
CCCEEEEECCCCCEECCCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCCHHHHH
DVANAVYDGADAVMLSAETAAGEWPEEAVTIMHKIAQQVERDPGYVERVRLLDTPPDPTT
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHEEEECCCCCCCH
ADALAHACMTIADTVPISAVTVFTGSGSTARRVARERPSVPMLVLTPHMRTARRLGLLWG
HHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHC
AHAVATKDIGSFEEMIAKGKRMALRHGFGKAGSKLIALAGVPFGTPGSTNLLHVVTLAGD
CHHHHHHCCCHHHHHHHCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCC
ELEKHGG
HHHHCCC
>Mature Secondary Structure 
SDHTKIDPRGRKVKILATVGPASREPEMLRRLFKAGADAFRVNMSHGDHATHAETIKAI
CCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHH
RELEREFYRPVAILCDLQGPKLRVGKFRDGKAVIRHSGHFTLDRNPEPGDETRVELPHPE
HHHHHHHCCCEEEEEECCCCCEEECCCCCCCEEEEECCEEEECCCCCCCCCCEEECCCHH
LFGLLSKGDRLLINDGKIRLKVKEADDERIFCSAEVGGVISDRKGVNVPDAEIPIPALTE
HHHHHCCCCEEEEECCEEEEEEEECCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCC
KDRKDLAFAMENGADWIGLSFVQRPEDLAEARKLMGGKGALCAKIEKPMAVRRLDELIEM
CCHHHHHHHHCCCCCEEEHHHHCCCHHHHHHHHHHCCCCCEEHHHCCCHHHHHHHHHHHH
SDGIMVARGDLGVELEPQEVPPLQKKIVNATRLKGKPVIVATQMLESMIESPAPTRAEVS
CCCEEEEECCCCCEECCCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCCHHHHH
DVANAVYDGADAVMLSAETAAGEWPEEAVTIMHKIAQQVERDPGYVERVRLLDTPPDPTT
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHEEEECCCCCCCH
ADALAHACMTIADTVPISAVTVFTGSGSTARRVARERPSVPMLVLTPHMRTARRLGLLWG
HHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHC
AHAVATKDIGSFEEMIAKGKRMALRHGFGKAGSKLIALAGVPFGTPGSTNLLHVVTLAGD
CHHHHHHCCCHHHHHHHCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCC
ELEKHGG
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA