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The map label for this gene is rpoN1 [H]

Identifier: 85375674

GI number: 85375674

Start: 2858218

End: 2859624

Strand: Reverse

Name: rpoN1 [H]

Synonym: ELI_14235

Alternate gene names: 85375674

Gene position: 2859624-2858218 (Counterclockwise)

Preceding gene: 85375675

Following gene: 85375673

Centisome position: 93.68

GC content: 63.26

Gene sequence:

>1407_bases
ATGACGCCGCAACTGCAGCAGGCGATCAAGCTGCTGGCGTTGAGCAATCTCGAAATCGAAAGTTTCATCGGCGACGCTCT
GGAATCCAATCCGCTGCTGGAAGCGGGCGAGGTAAGCCGCGAGCAGGGCGAGCCGGATGGCGACGTCCCCGTCTCACCGG
AAGAGGCGAGTTCCGACAGCTTCGGCAATGACGACTCGCTCGACATCAATACCAATGCGCTCGACCGCGATCGCGATACC
GGCGACGGCGAGTGGGGCGCGGGCGGAGCGGCATCCCACGCGAATGATTTGACGCCGATCGAGGAACGCCGCTCGAGCGA
GACGACCCTGGCAGATCACCTCCACCTGCAGGTCGGCACCCTCGCCTATAGCCAACGCGAAGAGTTCATCGCCCGCAACC
TGGTCGACCGGCTGGACGAGGCGGGCTATCTGCACGGCGACCTGCGCGAGATCGCTGTCGATCTCGGCGTCGAGCTGTAC
GAGATCGAGGATGCGCTCGAGCTGGTGCAGACGCTCGAGCCGACCGGCGTCGGCGCGCGCAGCCTGCAGGAATGCCTTGC
CCTGCAGGCCCGCGAAGCGGATCGTTTCGATCCCTGCATGCAGCGCATGATCGAGAACCTCGACCTGCTGGCCCAGGGCG
AAATCGCGCGGCTCAAGCGTATGTGCGATGTCGACGACGAGGATTTCGCCGACATGCTCACCGAGCTGCGCAGCTACGAC
CCCAAGCCGGGCCTCGCGTTCGGCGGGGGCGAAGCGGCTGCGGTGGTGCCCGACATCCTCGTGCGCCGCGACGAGGACGG
CCACTGGAAGATCCGCCTCAACGAAGCGACCCTGCCGCGACTGGTGGTCAATCGCAGCTATTTCCTCGAATTGCGCAACG
GTGCGACCGACAAGGCAAGCAAGAGCTGGCTCAAGGAAAAGCTCGGCGATGCCAATTGGTTGATCAAGGCGCTCGACCAG
CGCCAGAAAACCATCCTCGCCGTCTCGTCCGAGATCGTGAAGAAGCAGGCTGGCTTCTTCCGCAAGGGCGTCAGCGAATT
GAGGCCGCTCACTTTGCGCGAGATCGCCGAAGAGATCGAGATGCACGAGAGCACGGTCAGCCGCGTCACGAGCAACAAAT
ACCTCCACTGCGACCGCGGGACTTTCGAATTGAAGTACTTCTTTACCAGCGGTGTCGGCAGTGCCGATGGCGAAGGCGCG
AGCAGCGAGGCGGTGAAGGCGCGGATCAAGGCGCTGACCGATGCCGAAGACCCGAAAAAGATCCTCAGCGACGACAAACT
GGTGGAACTGCTCAAGGCCGAAGGCTTCGATATTGCCCGCCGTACCGTGGCCAAATACCGCGAGGCGATGGGAATCGGAA
GCTCCGTTCAGCGACGGCGAGCGAAGAAGCTGCAGGCGATCGGCTAG

Upstream 100 bases:

>100_bases
GCAGGACAGCCCTTCGACAGGCTCAGGGCGAACGGAATAGGGCTTATCGCTCGTGGCATTGGGCCCCCGCCTCGACCTTC
GCCAATCCCAGTCGCTGGTG

Downstream 100 bases:

>100_bases
CCGGAATTCTGGTTCCGCGACCTCCCAAGTGTTACCTTGTTGCAGCCTAATGTGTTGCGCCCAGCTTTGGCCAAATCAGC
TGAAAACCGACGTTTTACGG

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTPQLQQAIKLLALSNLEIESFIGDALESNPLLEAGEVSREQGEPDGDVPVSPEEASSDSFGNDDSLDINTNALDRDRDT
GDGEWGAGGAASHANDLTPIEERRSSETTLADHLHLQVGTLAYSQREEFIARNLVDRLDEAGYLHGDLREIAVDLGVELY
EIEDALELVQTLEPTGVGARSLQECLALQAREADRFDPCMQRMIENLDLLAQGEIARLKRMCDVDDEDFADMLTELRSYD
PKPGLAFGGGEAAAVVPDILVRRDEDGHWKIRLNEATLPRLVVNRSYFLELRNGATDKASKSWLKEKLGDANWLIKALDQ
RQKTILAVSSEIVKKQAGFFRKGVSELRPLTLREIAEEIEMHESTVSRVTSNKYLHCDRGTFELKYFFTSGVGSADGEGA
SSEAVKARIKALTDAEDPKKILSDDKLVELLKAEGFDIARRTVAKYREAMGIGSSVQRRRAKKLQAIG

Sequences:

>Translated_468_residues
MTPQLQQAIKLLALSNLEIESFIGDALESNPLLEAGEVSREQGEPDGDVPVSPEEASSDSFGNDDSLDINTNALDRDRDT
GDGEWGAGGAASHANDLTPIEERRSSETTLADHLHLQVGTLAYSQREEFIARNLVDRLDEAGYLHGDLREIAVDLGVELY
EIEDALELVQTLEPTGVGARSLQECLALQAREADRFDPCMQRMIENLDLLAQGEIARLKRMCDVDDEDFADMLTELRSYD
PKPGLAFGGGEAAAVVPDILVRRDEDGHWKIRLNEATLPRLVVNRSYFLELRNGATDKASKSWLKEKLGDANWLIKALDQ
RQKTILAVSSEIVKKQAGFFRKGVSELRPLTLREIAEEIEMHESTVSRVTSNKYLHCDRGTFELKYFFTSGVGSADGEGA
SSEAVKARIKALTDAEDPKKILSDDKLVELLKAEGFDIARRTVAKYREAMGIGSSVQRRRAKKLQAIG
>Mature_467_residues
TPQLQQAIKLLALSNLEIESFIGDALESNPLLEAGEVSREQGEPDGDVPVSPEEASSDSFGNDDSLDINTNALDRDRDTG
DGEWGAGGAASHANDLTPIEERRSSETTLADHLHLQVGTLAYSQREEFIARNLVDRLDEAGYLHGDLREIAVDLGVELYE
IEDALELVQTLEPTGVGARSLQECLALQAREADRFDPCMQRMIENLDLLAQGEIARLKRMCDVDDEDFADMLTELRSYDP
KPGLAFGGGEAAAVVPDILVRRDEDGHWKIRLNEATLPRLVVNRSYFLELRNGATDKASKSWLKEKLGDANWLIKALDQR
QKTILAVSSEIVKKQAGFFRKGVSELRPLTLREIAEEIEMHESTVSRVTSNKYLHCDRGTFELKYFFTSGVGSADGEGAS
SEAVKARIKALTDAEDPKKILSDDKLVELLKAEGFDIARRTVAKYREAMGIGSSVQRRRAKKLQAIG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=472, Percent_Identity=39.1949152542373, Blast_Score=320, Evalue=1e-88,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 51745; Mature: 51613

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPQLQQAIKLLALSNLEIESFIGDALESNPLLEAGEVSREQGEPDGDVPVSPEEASSDS
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCC
FGNDDSLDINTNALDRDRDTGDGEWGAGGAASHANDLTPIEERRSSETTLADHLHLQVGT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHH
LAYSQREEFIARNLVDRLDEAGYLHGDLREIAVDLGVELYEIEDALELVQTLEPTGVGAR
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHH
SLQECLALQAREADRFDPCMQRMIENLDLLAQGEIARLKRMCDVDDEDFADMLTELRSYD
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCC
PKPGLAFGGGEAAAVVPDILVRRDEDGHWKIRLNEATLPRLVVNRSYFLELRNGATDKAS
CCCCEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHCHHHEEEECCCCCCHHH
KSWLKEKLGDANWLIKALDQRQKTILAVSSEIVKKQAGFFRKGVSELRPLTLREIAEEIE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
MHESTVSRVTSNKYLHCDRGTFELKYFFTSGVGSADGEGASSEAVKARIKALTDAEDPKK
HHHHHHHHHHCCCEEEECCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHH
ILSDDKLVELLKAEGFDIARRTVAKYREAMGIGSSVQRRRAKKLQAIG
HHCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPQLQQAIKLLALSNLEIESFIGDALESNPLLEAGEVSREQGEPDGDVPVSPEEASSDS
CHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCC
FGNDDSLDINTNALDRDRDTGDGEWGAGGAASHANDLTPIEERRSSETTLADHLHLQVGT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHH
LAYSQREEFIARNLVDRLDEAGYLHGDLREIAVDLGVELYEIEDALELVQTLEPTGVGAR
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHH
SLQECLALQAREADRFDPCMQRMIENLDLLAQGEIARLKRMCDVDDEDFADMLTELRSYD
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCC
PKPGLAFGGGEAAAVVPDILVRRDEDGHWKIRLNEATLPRLVVNRSYFLELRNGATDKAS
CCCCEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHCHHHEEEECCCCCCHHH
KSWLKEKLGDANWLIKALDQRQKTILAVSSEIVKKQAGFFRKGVSELRPLTLREIAEEIE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
MHESTVSRVTSNKYLHCDRGTFELKYFFTSGVGSADGEGASSEAVKARIKALTDAEDPKK
HHHHHHHHHHCCCEEEECCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHH
ILSDDKLVELLKAEGFDIARRTVAKYREAMGIGSSVQRRRAKKLQAIG
HHCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1991712; 12597275 [H]