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The map label for this gene is trmB

Identifier: 85375671

GI number: 85375671

Start: 2855162

End: 2855863

Strand: Reverse

Name: trmB

Synonym: ELI_14220

Alternate gene names: 85375671

Gene position: 2855863-2855162 (Counterclockwise)

Preceding gene: 85375672

Following gene: 85375670

Centisome position: 93.56

GC content: 63.53

Gene sequence:

>702_bases
ATGACAGCGTTCAAGCAAGGCGACCCGACCACGCTCAACCGGCTCTACGGGCGCAGCATCGGCAAGCCGCTGCGCACGCA
TCAACAGCACCTGGTCGACAATCTGCTGCCGCAAATCTCACCGCCAGCCGAAGGCCCGGTTACGGCCGAGCGGTTGTTCG
GCGAGGACTGCCCGCTGCATTTCGAGATCGGCTTCGGCGGCGGGGAGCACCTGGTCTATCGCGCCGACCTGCTGCCCAAT
CATGGCTTCATCGGTGCCGAACCGTTTCTCAACGGCGTCGCCTCCTGCCTTTCACAGGTCGAGGAGCAGCGCCTTGCCAA
CATTCGCATCCATAATGGCGATGCGCTGGAAGTCCTCGATCGCGTGCCCGACGGCGCGCTGACCATGCTTTACCTGCTTC
ATCCCGACCCCTGGCCCAAGAACAAGCACGCCAAGCGGCGGATGATGAACGACGGGCCGGTGAAGATGTTCGCCGACAAG
CTCAAGCCCGGCGGGGAATTCCGCTTCGGCACCGACCACCCGGTCTACCTTCGCCACGCGCTGATGGTGATGCGGCGGCA
CACCGATGCTTTCGAATGGGTCGTGGAAGGTCCTTCCGACTGGCAGAACCGTCCGAGCGGCTGGCCCGAAACGCGCTACG
AACACAAGGCGCGGACCAAATTCGGCCATGAAGTCTGGTACTTCCGCTTCCGTCGGCGCTGA

Upstream 100 bases:

>100_bases
TGACCGACCAGATCGGTTGGGCCCAGGATTGGCTTGAAAGCCGCGCCGACTGAAAGACCGTCTGGACAGAAATCGGCCGC
CCCTTTAGCGAGCCGAGCCC

Downstream 100 bases:

>100_bases
AGCGCGCATTCGGGAACGATCTATTGCGCCACTCGCTTGCACTCACGGGAGGCCGTGAGGAGACATGCCATGGCGACCGC
CGATCTCGAATATCTCTATG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MTAFKQGDPTTLNRLYGRSIGKPLRTHQQHLVDNLLPQISPPAEGPVTAERLFGEDCPLHFEIGFGGGEHLVYRADLLPN
HGFIGAEPFLNGVASCLSQVEEQRLANIRIHNGDALEVLDRVPDGALTMLYLLHPDPWPKNKHAKRRMMNDGPVKMFADK
LKPGGEFRFGTDHPVYLRHALMVMRRHTDAFEWVVEGPSDWQNRPSGWPETRYEHKARTKFGHEVWYFRFRRR

Sequences:

>Translated_233_residues
MTAFKQGDPTTLNRLYGRSIGKPLRTHQQHLVDNLLPQISPPAEGPVTAERLFGEDCPLHFEIGFGGGEHLVYRADLLPN
HGFIGAEPFLNGVASCLSQVEEQRLANIRIHNGDALEVLDRVPDGALTMLYLLHPDPWPKNKHAKRRMMNDGPVKMFADK
LKPGGEFRFGTDHPVYLRHALMVMRRHTDAFEWVVEGPSDWQNRPSGWPETRYEHKARTKFGHEVWYFRFRRR
>Mature_232_residues
TAFKQGDPTTLNRLYGRSIGKPLRTHQQHLVDNLLPQISPPAEGPVTAERLFGEDCPLHFEIGFGGGEHLVYRADLLPNH
GFIGAEPFLNGVASCLSQVEEQRLANIRIHNGDALEVLDRVPDGALTMLYLLHPDPWPKNKHAKRRMMNDGPVKMFADKL
KPGGEFRFGTDHPVYLRHALMVMRRHTDAFEWVVEGPSDWQNRPSGWPETRYEHKARTKFGHEVWYFRFRRR

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=230, Percent_Identity=35.2173913043478, Blast_Score=124, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_ERYLH (Q2N5V5)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_459733.1
- ProteinModelPortal:   Q2N5V5
- SMR:   Q2N5V5
- STRING:   Q2N5V5
- GeneID:   3870854
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_14220
- NMPDR:   fig|314225.3.peg.174
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   RYGRKAE
- PhylomeDB:   Q2N5V5
- ProtClustDB:   PRK00121
- BioCyc:   ELIT314225:ELI_14220-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 26810; Mature: 26679

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: BINDING 62-62 BINDING 87-87 BINDING 114-114 BINDING 136-136 BINDING 140-140 BINDING 172-172

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFKQGDPTTLNRLYGRSIGKPLRTHQQHLVDNLLPQISPPAEGPVTAERLFGEDCPLH
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCEE
FEIGFGGGEHLVYRADLLPNHGFIGAEPFLNGVASCLSQVEEQRLANIRIHNGDALEVLD
EEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHH
RVPDGALTMLYLLHPDPWPKNKHAKRRMMNDGPVKMFADKLKPGGEFRFGTDHPVYLRHA
HCCCCCEEEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHH
LMVMRRHTDAFEWVVEGPSDWQNRPSGWPETRYEHKARTKFGHEVWYFRFRRR
HHHHHHCCCHHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECC
>Mature Secondary Structure 
TAFKQGDPTTLNRLYGRSIGKPLRTHQQHLVDNLLPQISPPAEGPVTAERLFGEDCPLH
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCEE
FEIGFGGGEHLVYRADLLPNHGFIGAEPFLNGVASCLSQVEEQRLANIRIHNGDALEVLD
EEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHH
RVPDGALTMLYLLHPDPWPKNKHAKRRMMNDGPVKMFADKLKPGGEFRFGTDHPVYLRHA
HCCCCCEEEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHH
LMVMRRHTDAFEWVVEGPSDWQNRPSGWPETRYEHKARTKFGHEVWYFRFRRR
HHHHHHCCCHHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA