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The map label for this gene is cysD [H]

Identifier: 85375562

GI number: 85375562

Start: 2756440

End: 2757345

Strand: Reverse

Name: cysD [H]

Synonym: ELI_13675

Alternate gene names: 85375562

Gene position: 2757345-2756440 (Counterclockwise)

Preceding gene: 85375563

Following gene: 85375561

Centisome position: 90.33

GC content: 63.91

Gene sequence:

>906_bases
ATGACCACGCTCCTGACCCATCTCCAGCGGCTCGAGGCCGAGGCGATCCACATCATGCGCGAAGTGGTGGCCGAAACGCA
GAACCCGGTCATGCTCTATTCGGTGGGCAAGGACAGCTCGGTGATGCTGCACCTGGCGCGCAAGGCCTTCTACCCGGCCC
CGCCGCCTTTCCCGATGCTGCATGTCGCCAGCGGCTGGGATTTCCGGGACCTGCTCGCCCATCGCGACCGCACGGTTCGG
GAATACGGGCTGGAGCTGATCGTCGCCGAGAACGAAGAAGCCGAAGAGCAAGGCATAAATCCCTTCGACACCGGCTCGGC
GCTCTACAGCCAGGCGCAGTTGACCGACCCGCTCAAGCGCGCGCTCAATGAACACGGCTTCGACGCGGCCTTCGGCGGCG
GTCGGCGCGACGAGGAAAAAGCGCGCGCCAAGGAGCGTATCTTCAGCTTCCGCAACGCCGCGCATCGCTGGGACCCGAAG
AACCAGCGGCCCGAGCTGTGGAACCTCTACAACACGCGCAAGAAGGACGGCGAGAGCATCCGCGTCTTCCCGCTCTCCAA
CTGGACCGAGCTCGATATCTGGCAATATATCATGGCCGAGCAGATCGATATCGTGCCGCTCTATTATGCTTCGCGGCGAC
CCACCGTGATGCGCGACGGCACGCTGCTGGTCGTCGATGACGAGCGCTTCCGGTTCGAGGTTGGCGAGCAAGCGGTCGAA
CGCACCGTGCGTTTTCGGACGCTGGGCTGCTACCCGCTGACCGGGGCCGTCGAAAGCGAGGCAACCGAGCTCAGCGACAT
CATCCAGGAGATGCTGCTCGCGACCGGGTCCGAACGCATGGGCCGCGCGATCGACAAGGGCGAAAGCGCCTCGATGGAAG
CCAAGAAGCAGGAGGGATACTTTTGA

Upstream 100 bases:

>100_bases
AAGCGGCGAATTTGCCCGCCCGTCTTGTGACGGCCCATTCCAAGGTGCAAAGCTTTCCTTTGTTGCGCGACGCTCAAGGG
AATCTACCTCCGCCCTCCTG

Downstream 100 bases:

>100_bases
CCTGCCACGTCAGACACGAAGTGGCTGATGAAAGGTCAACACCGGACTTGATCCGGGGTCTCCATCCGCGCGCGCAACAC
AAGCTGGATTCCCGTCTGCG

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MTTLLTHLQRLEAEAIHIMREVVAETQNPVMLYSVGKDSSVMLHLARKAFYPAPPPFPMLHVASGWDFRDLLAHRDRTVR
EYGLELIVAENEEAEEQGINPFDTGSALYSQAQLTDPLKRALNEHGFDAAFGGGRRDEEKARAKERIFSFRNAAHRWDPK
NQRPELWNLYNTRKKDGESIRVFPLSNWTELDIWQYIMAEQIDIVPLYYASRRPTVMRDGTLLVVDDERFRFEVGEQAVE
RTVRFRTLGCYPLTGAVESEATELSDIIQEMLLATGSERMGRAIDKGESASMEAKKQEGYF

Sequences:

>Translated_301_residues
MTTLLTHLQRLEAEAIHIMREVVAETQNPVMLYSVGKDSSVMLHLARKAFYPAPPPFPMLHVASGWDFRDLLAHRDRTVR
EYGLELIVAENEEAEEQGINPFDTGSALYSQAQLTDPLKRALNEHGFDAAFGGGRRDEEKARAKERIFSFRNAAHRWDPK
NQRPELWNLYNTRKKDGESIRVFPLSNWTELDIWQYIMAEQIDIVPLYYASRRPTVMRDGTLLVVDDERFRFEVGEQAVE
RTVRFRTLGCYPLTGAVESEATELSDIIQEMLLATGSERMGRAIDKGESASMEAKKQEGYF
>Mature_300_residues
TTLLTHLQRLEAEAIHIMREVVAETQNPVMLYSVGKDSSVMLHLARKAFYPAPPPFPMLHVASGWDFRDLLAHRDRTVRE
YGLELIVAENEEAEEQGINPFDTGSALYSQAQLTDPLKRALNEHGFDAAFGGGRRDEEKARAKERIFSFRNAAHRWDPKN
QRPELWNLYNTRKKDGESIRVFPLSNWTELDIWQYIMAEQIDIVPLYYASRRPTVMRDGTLLVVDDERFRFEVGEQAVER
TVRFRTLGCYPLTGAVESEATELSDIIQEMLLATGSERMGRAIDKGESASMEAKKQEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=297, Percent_Identity=64.983164983165, Blast_Score=407, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 34517; Mature: 34386

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLLTHLQRLEAEAIHIMREVVAETQNPVMLYSVGKDSSVMLHLARKAFYPAPPPFPML
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCEE
HVASGWDFRDLLAHRDRTVREYGLELIVAENEEAEEQGINPFDTGSALYSQAQLTDPLKR
EECCCCCHHHHHHHCCHHHHHHCCEEEEECCCCHHHCCCCCCCCCHHHHHHHHHHHHHHH
ALNEHGFDAAFGGGRRDEEKARAKERIFSFRNAAHRWDPKNQRPELWNLYNTRKKDGESI
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCEE
RVFPLSNWTELDIWQYIMAEQIDIVPLYYASRRPTVMRDGTLLVVDDERFRFEVGEQAVE
EEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEECCEEEEEECCHHHHHHHHHHHH
RTVRFRTLGCYPLTGAVESEATELSDIIQEMLLATGSERMGRAIDKGESASMEAKKQEGY
HHHHHHEECCEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHCCC
F
C
>Mature Secondary Structure 
TTLLTHLQRLEAEAIHIMREVVAETQNPVMLYSVGKDSSVMLHLARKAFYPAPPPFPML
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCEE
HVASGWDFRDLLAHRDRTVREYGLELIVAENEEAEEQGINPFDTGSALYSQAQLTDPLKR
EECCCCCHHHHHHHCCHHHHHHCCEEEEECCCCHHHCCCCCCCCCHHHHHHHHHHHHHHH
ALNEHGFDAAFGGGRRDEEKARAKERIFSFRNAAHRWDPKNQRPELWNLYNTRKKDGESI
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCEE
RVFPLSNWTELDIWQYIMAEQIDIVPLYYASRRPTVMRDGTLLVVDDERFRFEVGEQAVE
EEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEECCEEEEEECCHHHHHHHHHHHH
RTVRFRTLGCYPLTGAVESEATELSDIIQEMLLATGSERMGRAIDKGESASMEAKKQEGY
HHHHHHEECCEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]