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The map label for this gene is ribF [H]

Identifier: 85375539

GI number: 85375539

Start: 2733356

End: 2734303

Strand: Reverse

Name: ribF [H]

Synonym: ELI_13560

Alternate gene names: 85375539

Gene position: 2734303-2733356 (Counterclockwise)

Preceding gene: 85375540

Following gene: 85375538

Centisome position: 89.58

GC content: 64.14

Gene sequence:

>948_bases
ATGCGCTGGCTCGACCATCGAAACCCCGTCCCTGAATCCTTGCGCGGTGCCATTATCGCGCTGGGCAATTTCGACGGCTT
CCATCGCGGCCACCAGGCGGTGGCTGGCGAAGCCATCGACTGGGCGCATGCAGAAGGCCGCCCGAGCATCATCGCGACTT
TCGATCCGCACCCGGTGCGCTTCTTCCGCCCCGATACACCGCCCTTCCGCCTGACAACGCTGGAGCAGCGGCAGGAGCTC
TATATCGCCGCCGGGGCAACCGCGATGCTGGTGTTCCATTTCGACAAGGAGCTCGCCAGCACCAGCGCGGAAGACTTCAT
CACCGAGATCCTGATCGACCGCCTCGGTGCGCATGGCGTGGTGACGGGCGGCGATTTCACCTTCGGCCAGGGCGCCAAGG
GCAATGTCGATCTGCTTAAGAGCTTCGGCGGCGAGCGCGGTCTCGAGAGCCGTGTCGTCGAGCCGGTCGAAGATGCGGGT
TTGGGTAACGGGGGCGTCATCTCCTCCAGCCGCATCCGCGAGGCCTTGCGCGACGGCGATTGCGCCACCGCGACCGAACT
GCTGTCGCGCCCCTTCGCCATTCGGGGCGTGGTCGAGCACGGCGACAAGCGCGGCCGCGAAATCGGCTATCCGACCGCGA
ACCTGCATATCGAGAGCTATTTGCGCCCGCGCTACGGCATCTATGCCGTGACGGGGCGCATTCTTGCCACCGGTCAGGAA
CTCACGGGCGCGGCGAACATCGGCATCCGGCCGCAGTTCGAGCCTCCCAAGGAGCTGCTCGAACCCTATTTCTTCGACTT
TTCCGGCGACCTCTACGGGCAGGAGATCGAAGTTTCCTTCCACCACTTCCTGCGCCCGGAGGCGAAGTTCGACAGTCTAG
AAGCGCTGATGGCGCAGATGGAAAAGGATTGCGAACGGGCACGCGAATTGCTCGCGCACAAAGGGTAA

Upstream 100 bases:

>100_bases
AGCGTGAAATCCGCGCCGTGATGGGCGGCAATGCCATCCGCGTGCTGCGCGAAGGGATGAAGCCGCTTGCGGATGTCGCG
CAGCCAGCTACCAATTCCTG

Downstream 100 bases:

>100_bases
GCCTGGAACATCGGCCCGCCATGGCGGGTTGATCGATCAGCGCTGCACCCTGCCTCGCTTTTCCGTGGAGACAATGATGA
CCCCCGCCCAGCTCGAAGCC

Product: riboflavin biosynthesis protein

Products: NA

Alternate protein names: Riboflavin kinase; Flavokinase; FMN adenylyltransferase; FAD pyrophosphorylase; FAD synthase [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MRWLDHRNPVPESLRGAIIALGNFDGFHRGHQAVAGEAIDWAHAEGRPSIIATFDPHPVRFFRPDTPPFRLTTLEQRQEL
YIAAGATAMLVFHFDKELASTSAEDFITEILIDRLGAHGVVTGGDFTFGQGAKGNVDLLKSFGGERGLESRVVEPVEDAG
LGNGGVISSSRIREALRDGDCATATELLSRPFAIRGVVEHGDKRGREIGYPTANLHIESYLRPRYGIYAVTGRILATGQE
LTGAANIGIRPQFEPPKELLEPYFFDFSGDLYGQEIEVSFHHFLRPEAKFDSLEALMAQMEKDCERARELLAHKG

Sequences:

>Translated_315_residues
MRWLDHRNPVPESLRGAIIALGNFDGFHRGHQAVAGEAIDWAHAEGRPSIIATFDPHPVRFFRPDTPPFRLTTLEQRQEL
YIAAGATAMLVFHFDKELASTSAEDFITEILIDRLGAHGVVTGGDFTFGQGAKGNVDLLKSFGGERGLESRVVEPVEDAG
LGNGGVISSSRIREALRDGDCATATELLSRPFAIRGVVEHGDKRGREIGYPTANLHIESYLRPRYGIYAVTGRILATGQE
LTGAANIGIRPQFEPPKELLEPYFFDFSGDLYGQEIEVSFHHFLRPEAKFDSLEALMAQMEKDCERARELLAHKG
>Mature_315_residues
MRWLDHRNPVPESLRGAIIALGNFDGFHRGHQAVAGEAIDWAHAEGRPSIIATFDPHPVRFFRPDTPPFRLTTLEQRQEL
YIAAGATAMLVFHFDKELASTSAEDFITEILIDRLGAHGVVTGGDFTFGQGAKGNVDLLKSFGGERGLESRVVEPVEDAG
LGNGGVISSSRIREALRDGDCATATELLSRPFAIRGVVEHGDKRGREIGYPTANLHIESYLRPRYGIYAVTGRILATGQE
LTGAANIGIRPQFEPPKELLEPYFFDFSGDLYGQEIEVSFHHFLRPEAKFDSLEALMAQMEKDCERARELLAHKG

Specific function: Unknown

COG id: COG0196

COG function: function code H; FAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribF family [H]

Homologues:

Organism=Escherichia coli, GI1786208, Length=310, Percent_Identity=35.8064516129032, Blast_Score=194, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015864
- InterPro:   IPR015865
- InterPro:   IPR002606
- InterPro:   IPR014729 [H]

Pfam domain/function: PF06574 FAD_syn; PF01687 Flavokinase [H]

EC number: =2.7.1.26; =2.7.7.2 [H]

Molecular weight: Translated: 34785; Mature: 34785

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWLDHRNPVPESLRGAIIALGNFDGFHRGHQAVAGEAIDWAHAEGRPSIIATFDPHPVR
CCCCCCCCCCCHHHCCEEEEEECCCCHHHHHHHHHCCCHHHHCCCCCCCEEEEECCCCEE
FFRPDTPPFRLTTLEQRQELYIAAGATAMLVFHFDKELASTSAEDFITEILIDRLGAHGV
EECCCCCCEEEEEHHHCCEEEEEECCEEEEEEEECHHHHHCCHHHHHHHHHHHHCCCCCE
VTGGDFTFGQGAKGNVDLLKSFGGERGLESRVVEPVEDAGLGNGGVISSSRIREALRDGD
EECCCCCCCCCCCCCHHHHHHCCCCCCCHHHCCCCHHHCCCCCCCCCCHHHHHHHHHCCC
CATATELLSRPFAIRGVVEHGDKRGREIGYPTANLHIESYLRPRYGIYAVTGRILATGQE
HHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCCCEEHHHHCCCCCCEEEEECCCEECCHH
LTGAANIGIRPQFEPPKELLEPYFFDFSGDLYGQEIEVSFHHFLRPEAKFDSLEALMAQM
HCCCCCCCCCCCCCCHHHHHCHHEECCCCCCCCCEEEEEHHHHCCCCCCHHHHHHHHHHH
EKDCERARELLAHKG
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MRWLDHRNPVPESLRGAIIALGNFDGFHRGHQAVAGEAIDWAHAEGRPSIIATFDPHPVR
CCCCCCCCCCCHHHCCEEEEEECCCCHHHHHHHHHCCCHHHHCCCCCCCEEEEECCCCEE
FFRPDTPPFRLTTLEQRQELYIAAGATAMLVFHFDKELASTSAEDFITEILIDRLGAHGV
EECCCCCCEEEEEHHHCCEEEEEECCEEEEEEEECHHHHHCCHHHHHHHHHHHHCCCCCE
VTGGDFTFGQGAKGNVDLLKSFGGERGLESRVVEPVEDAGLGNGGVISSSRIREALRDGD
EECCCCCCCCCCCCCHHHHHHCCCCCCCHHHCCCCHHHCCCCCCCCCCHHHHHHHHHCCC
CATATELLSRPFAIRGVVEHGDKRGREIGYPTANLHIESYLRPRYGIYAVTGRILATGQE
HHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCCCEEHHHHCCCCCCEEEEECCCEECCHH
LTGAANIGIRPQFEPPKELLEPYFFDFSGDLYGQEIEVSFHHFLRPEAKFDSLEALMAQM
HCCCCCCCCCCCCCCHHHHHCHHEECCCCCCCCCEEEEEHHHHCCCCCCHHHHHHHHHHH
EKDCERARELLAHKG
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]