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The map label for this gene is fcl [C]

Identifier: 85375519

GI number: 85375519

Start: 2711734

End: 2712678

Strand: Reverse

Name: fcl [C]

Synonym: ELI_13460

Alternate gene names: 85375519

Gene position: 2712678-2711734 (Counterclockwise)

Preceding gene: 85375520

Following gene: 85375517

Centisome position: 88.87

GC content: 61.8

Gene sequence:

>945_bases
TTGACCGACCTGCCCTTCGCGCTCGCGGGCAAGCGTGTTTATGTTGCGGGCCATCGCGGCATGGTCGGCTCGGCGCTGGT
GCGCCGCTTGCGGCACGAAAACTGCCTCGTCCAGACCGCCGATCGTTCGGTGGACCTGCGCGAACAGGCGCATGTGCGCG
GCTGGTTTGCGGACAACCGGCCCGATGTCGTCATCGTCGCAGCGGCGAGGGTCGGCGGGATCCTCGCCAACGAGAGCTAT
CCGGCGGAATTTCTATACGACAATCTGATGATCGAAGCGAACCTGATCGAGGCCGCGCATCGTCATGATACCGAAAAGCT
GCTCTTCCTCGGATCCTCCTGCATCTACCCGAAACTTGCCCCGCAGCCGATCGCGGAAGACGCGCTTCTGACAGGCCCGC
TCGAACCGACCAACGAATGGTATGCCATCGCCAAGATCGCCGGGATCAAGCTGTGCCAAGCCTATCGCCGCCAGTATGGC
GCGAACTTCATCAGTGCGATGCCGACCAATCTCTATGGGCCGGGCGACAATTTCGACGAGAAATCGAGCCATGTGCTGCC
CGCGCTGATTCGCAAGGCGCACGCGGCCAAGCTGGCGGGCGACAGCGCAATCACGGTTTGGGGTACGGGCACGCCGCGAC
GCGAATTCCTGCATGTCGACGATCTCGCCGCAGCCTGCATTTTCCTGCTCCAGAATTATTCGGGCGAAAGCCATGTCAAC
GTGGGGTCGGGTAGCGACCTGACCATCAATGAACTGGCCGAAACCGTGTGCAAGGTCGTGCGATTCGAAGGCACCATCGA
ACATGACACCTCCAGGCCCGACGGCACGCCGCGCAAGCTCATGGACGGGTCGACAATCACCGCGATGGGCTGGAAACCGA
CGATCGACCTCGAAGATGGGATCGCCCAGGCCTATCGCTGGTTTGTCGACAACGAAACCGCCTAG

Upstream 100 bases:

>100_bases
AGCAGCCCGAATTCGGCGTCGCGCCGGGGCAGGCGGGGGTGATCTATGCCGGCGATCGGGTCATCGGTGGCGGCTGGATC
GACAGCACAGAGAGCGCAGC

Downstream 100 bases:

>100_bases
GCCGGGGCGTCAGCCCTCCCCTCACCCCTCCCAAGGCTCCAGCACGCAGCCATAGCCATCGCGCAGCCGCGCGGTATCGC
TGGCGAGCAGCAGGAACGAG

Product: GDP-fucose synthetase

Products: GDP-4-dehydro-6-deoxy-D-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MTDLPFALAGKRVYVAGHRGMVGSALVRRLRHENCLVQTADRSVDLREQAHVRGWFADNRPDVVIVAAARVGGILANESY
PAEFLYDNLMIEANLIEAAHRHDTEKLLFLGSSCIYPKLAPQPIAEDALLTGPLEPTNEWYAIAKIAGIKLCQAYRRQYG
ANFISAMPTNLYGPGDNFDEKSSHVLPALIRKAHAAKLAGDSAITVWGTGTPRREFLHVDDLAAACIFLLQNYSGESHVN
VGSGSDLTINELAETVCKVVRFEGTIEHDTSRPDGTPRKLMDGSTITAMGWKPTIDLEDGIAQAYRWFVDNETA

Sequences:

>Translated_314_residues
MTDLPFALAGKRVYVAGHRGMVGSALVRRLRHENCLVQTADRSVDLREQAHVRGWFADNRPDVVIVAAARVGGILANESY
PAEFLYDNLMIEANLIEAAHRHDTEKLLFLGSSCIYPKLAPQPIAEDALLTGPLEPTNEWYAIAKIAGIKLCQAYRRQYG
ANFISAMPTNLYGPGDNFDEKSSHVLPALIRKAHAAKLAGDSAITVWGTGTPRREFLHVDDLAAACIFLLQNYSGESHVN
VGSGSDLTINELAETVCKVVRFEGTIEHDTSRPDGTPRKLMDGSTITAMGWKPTIDLEDGIAQAYRWFVDNETA
>Mature_313_residues
TDLPFALAGKRVYVAGHRGMVGSALVRRLRHENCLVQTADRSVDLREQAHVRGWFADNRPDVVIVAAARVGGILANESYP
AEFLYDNLMIEANLIEAAHRHDTEKLLFLGSSCIYPKLAPQPIAEDALLTGPLEPTNEWYAIAKIAGIKLCQAYRRQYGA
NFISAMPTNLYGPGDNFDEKSSHVLPALIRKAHAAKLAGDSAITVWGTGTPRREFLHVDDLAAACIFLLQNYSGESHVNV
GSGSDLTINELAETVCKVVRFEGTIEHDTSRPDGTPRKLMDGSTITAMGWKPTIDLEDGIAQAYRWFVDNETA

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=308, Percent_Identity=34.0909090909091, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI7657641, Length=341, Percent_Identity=23.4604105571848, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI42516563, Length=323, Percent_Identity=27.8637770897833, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788365, Length=316, Percent_Identity=54.1139240506329, Blast_Score=350, Evalue=7e-98,
Organism=Escherichia coli, GI48994969, Length=349, Percent_Identity=24.3553008595989, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788353, Length=352, Percent_Identity=26.7045454545455, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17554428, Length=316, Percent_Identity=31.9620253164557, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI115532424, Length=269, Percent_Identity=26.0223048327138, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI19922778, Length=314, Percent_Identity=31.2101910828025, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI21356223, Length=325, Percent_Identity=27.3846153846154, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.271

Molecular weight: Translated: 34463; Mature: 34332

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLPFALAGKRVYVAGHRGMVGSALVRRLRHENCLVQTADRSVDLREQAHVRGWFADNR
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHEEEEECCCC
PDVVIVAAARVGGILANESYPAEFLYDNLMIEANLIEAAHRHDTEKLLFLGSSCIYPKLA
CCEEEEEEHHHCCEEECCCCCHHHHHHHEEEEHHHHHHHHHCCCCEEEEECCCCCCCCCC
PQPIAEDALLTGPLEPTNEWYAIAKIAGIKLCQAYRRQYGANFISAMPTNLYGPGDNFDE
CCCCCCCCEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCC
KSSHVLPALIRKAHAAKLAGDSAITVWGTGTPRREFLHVDDLAAACIFLLQNYSGESHVN
CCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHEEEHHHHHHHHHHHHHCCCCCCEEE
VGSGSDLTINELAETVCKVVRFEGTIEHDTSRPDGTPRKLMDGSTITAMGWKPTIDLEDG
CCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCEEEEECCCCCCCHHHH
IAQAYRWFVDNETA
HHHHHHHHHCCCCC
>Mature Secondary Structure 
TDLPFALAGKRVYVAGHRGMVGSALVRRLRHENCLVQTADRSVDLREQAHVRGWFADNR
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHEEEEECCCC
PDVVIVAAARVGGILANESYPAEFLYDNLMIEANLIEAAHRHDTEKLLFLGSSCIYPKLA
CCEEEEEEHHHCCEEECCCCCHHHHHHHEEEEHHHHHHHHHCCCCEEEEECCCCCCCCCC
PQPIAEDALLTGPLEPTNEWYAIAKIAGIKLCQAYRRQYGANFISAMPTNLYGPGDNFDE
CCCCCCCCEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCC
KSSHVLPALIRKAHAAKLAGDSAITVWGTGTPRREFLHVDDLAAACIFLLQNYSGESHVN
CCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHEEEHHHHHHHHHHHHHCCCCCCEEE
VGSGSDLTINELAETVCKVVRFEGTIEHDTSRPDGTPRKLMDGSTITAMGWKPTIDLEDG
CCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCEEEEECCCCCCCHHHH
IAQAYRWFVDNETA
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-L-fucose; NADP+

Specific reaction: GDP-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-D-mannose + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]