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The map label for this gene is fabI1 [H]

Identifier: 85375508

GI number: 85375508

Start: 2701933

End: 2702745

Strand: Reverse

Name: fabI1 [H]

Synonym: ELI_13405

Alternate gene names: 85375508

Gene position: 2702745-2701933 (Counterclockwise)

Preceding gene: 85375509

Following gene: 85375506

Centisome position: 88.54

GC content: 60.52

Gene sequence:

>813_bases
ATGAGTAAGAAACAGGGCCTGATGGCCGGAAAACGCGGCTTGATCATGGGTCTGGCCAATGACAAGTCGCTGGCCTGGGG
CATCGCCAAACAGCTCGCCGAGCACGGCGCGGAACTGGCCTTCTCCTATCAGGGCGAGGCCCTCGCCAAGCGGGTCAAGC
CACTCGCCGAGCAACTGGGTAGCGACTTCACATTCGATTGCGACGTGTCGGACATGGCAGCGCTCGACCGCGCTTTCGAC
ACGCTGAAGCACCGCTGGGACAGCATCGATTTCGTCGTCCACGCGATCGGCTTTTCCGACAAGAACGAACTGCGCGGAAA
ATATATCGACACCAGCCTCGACAATTTCCTGATGACGATGAACATCTCCGCCTATTCGCTGGTCGCCGTGGCCAAGCGCG
CGGCCGAGATGATGCCGGACGGTGGCTCGATCCTGACGCTGACCTATTACGGCGCGGAAAAGGTGATTCCGCATTACAAC
GTCATGGGCGTCGCCAAGGCCGCGCTGGAAACCAGCGTGATGTATCTCGCCAACGACCTTGGCCCGCAGAACATCCGCGT
CAACGCGATCAGCGCGGGGCCGGTCAAGACGCTCGCTGCCAGCGGTATCGGCGATTTCCGTTACATCCTCAAATGGAACG
AGCTCAACTCGCCCCTGCGCCGCAATATCACGATCGACGATGTCGGCGGATCGGGGCTGTATTTCCTTTCCCACCTGTCC
TCCGGCGTGACCGGCGAAACGCATCACGTCGATGCCGGCTATCACATCGTCGGCATGAAGCAGGAGGATGCGCCGGATAT
CGCCACGAGCTGA

Upstream 100 bases:

>100_bases
CTTGCGGTCAGCCCGGAAGAAGAGGAAAACATCATCGGACAATGGGACGACAAGTCGCGAAAACGCCGCAACGGCGCGTC
CCCTCGCGAGGGAACCGGAT

Downstream 100 bases:

>100_bases
CCTTCGCTAGCCTTCCAGTGTCCGGACGCACGCGATTGCGATCCGCGCCTGTCGAGCGGCCTGTTGGTCATCCGGCAGCT
CTGCCAATTCGTCCTCTTGC

Product: enoyl-[acyl-carrier-protein] reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MSKKQGLMAGKRGLIMGLANDKSLAWGIAKQLAEHGAELAFSYQGEALAKRVKPLAEQLGSDFTFDCDVSDMAALDRAFD
TLKHRWDSIDFVVHAIGFSDKNELRGKYIDTSLDNFLMTMNISAYSLVAVAKRAAEMMPDGGSILTLTYYGAEKVIPHYN
VMGVAKAALETSVMYLANDLGPQNIRVNAISAGPVKTLAASGIGDFRYILKWNELNSPLRRNITIDDVGGSGLYFLSHLS
SGVTGETHHVDAGYHIVGMKQEDAPDIATS

Sequences:

>Translated_270_residues
MSKKQGLMAGKRGLIMGLANDKSLAWGIAKQLAEHGAELAFSYQGEALAKRVKPLAEQLGSDFTFDCDVSDMAALDRAFD
TLKHRWDSIDFVVHAIGFSDKNELRGKYIDTSLDNFLMTMNISAYSLVAVAKRAAEMMPDGGSILTLTYYGAEKVIPHYN
VMGVAKAALETSVMYLANDLGPQNIRVNAISAGPVKTLAASGIGDFRYILKWNELNSPLRRNITIDDVGGSGLYFLSHLS
SGVTGETHHVDAGYHIVGMKQEDAPDIATS
>Mature_269_residues
SKKQGLMAGKRGLIMGLANDKSLAWGIAKQLAEHGAELAFSYQGEALAKRVKPLAEQLGSDFTFDCDVSDMAALDRAFDT
LKHRWDSIDFVVHAIGFSDKNELRGKYIDTSLDNFLMTMNISAYSLVAVAKRAAEMMPDGGSILTLTYYGAEKVIPHYNV
MGVAKAALETSVMYLANDLGPQNIRVNAISAGPVKTLAASGIGDFRYILKWNELNSPLRRNITIDDVGGSGLYFLSHLSS
GVTGETHHVDAGYHIVGMKQEDAPDIATS

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=257, Percent_Identity=48.2490272373541, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI2367365, Length=253, Percent_Identity=27.6679841897233, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789378, Length=256, Percent_Identity=26.5625, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 29252; Mature: 29120

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKQGLMAGKRGLIMGLANDKSLAWGIAKQLAEHGAELAFSYQGEALAKRVKPLAEQLG
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHC
SDFTFDCDVSDMAALDRAFDTLKHRWDSIDFVVHAIGFSDKNELRGKYIDTSLDNFLMTM
CCCEEECCHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCHHCCCEEECCCHHCEEEEE
NISAYSLVAVAKRAAEMMPDGGSILTLTYYGAEKVIPHYNVMGVAKAALETSVMYLANDL
CCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHCCCCCCHHHHHHHHHHHHHHHHHHCC
GPQNIRVNAISAGPVKTLAASGIGDFRYILKWNELNSPLRRNITIDDVGGSGLYFLSHLS
CCCCEEEEEECCCCHHHHHHCCCCCEEEEEEECCCCCHHHCCCEEECCCCCHHHHHHHHC
SGVTGETHHVDAGYHIVGMKQEDAPDIATS
CCCCCCCEEECCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SKKQGLMAGKRGLIMGLANDKSLAWGIAKQLAEHGAELAFSYQGEALAKRVKPLAEQLG
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHC
SDFTFDCDVSDMAALDRAFDTLKHRWDSIDFVVHAIGFSDKNELRGKYIDTSLDNFLMTM
CCCEEECCHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCHHCCCEEECCCHHCEEEEE
NISAYSLVAVAKRAAEMMPDGGSILTLTYYGAEKVIPHYNVMGVAKAALETSVMYLANDL
CCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHCCCCCCHHHHHHHHHHHHHHHHHHCC
GPQNIRVNAISAGPVKTLAASGIGDFRYILKWNELNSPLRRNITIDDVGGSGLYFLSHLS
CCCCEEEEEECCCCHHHHHHCCCCCEEEEEEECCCCCHHHCCCEEECCCCCHHHHHHHHC
SGVTGETHHVDAGYHIVGMKQEDAPDIATS
CCCCCCCEEECCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]