Click here to switch to the map view.

The map label for this gene is capL [H]

Identifier: 85375486

GI number: 85375486

Start: 2677322

End: 2678647

Strand: Direct

Name: capL [H]

Synonym: ELI_13295

Alternate gene names: 85375486

Gene position: 2677322-2678647 (Clockwise)

Preceding gene: 85375485

Following gene: 85375487

Centisome position: 87.71

GC content: 59.35

Gene sequence:

>1326_bases
ATGACAGCGACCCTCTCGTCCATCGCGCGCGAAACGATTGCCCGCTTCGAGAACAAATCGGCGAAGATCGGCATTCTTGG
CCTTGGCTATGTCGGCATCCCTCTGGCGCTGCGCTTCAGTGAAGTCGGCCTGACCGTACTGGGTTTCGACGTCGATCGGG
AGCGCGTGCGCGAACTGAATGCCGGTGAATCTCCGATCAAGCACATCCCCGCCAGCGAGATCGAGGGCATGAAGCGCACT
GGTTTCGAGGCGACAGACGATTTCTCGCGAATTCCTGAAGTCGACGCGATCATCATCTGTGTCCCGACGCCGCTCAGCGA
ACAGCGCGAGCCCGACTTGTCCTATGTCGTCAGCACAATGGAGTCGGTAAAAGGCCACCTGCGCGAAGGGCAATTGCTGT
CGCTCGAGAGCACGACGTGGCCCGGGACCACCGACGAAGTACTCAAGCCCTATATCGATGCGCGCGGCCTCACCGTCGGG
GAGGACTTCTTTCTTGTCTATTCGCCTGAGCGCGAAGATCCGGGCAACCCGGACTTCACCACCCGTACGATTCCCAAGGT
GCTGGGGGGCGTCACGGAAACATGCAGCGCTGTCGGCATGGCGCTGTATGGCACGGTGATCGACAAGGTTGTGCCGGTTT
CTTCGACACAGGCGGCCGAGCTGACCAAGCTGCTCGAGAACATCCACCGCTCGGTCAACATCGGTCTCGTGAACGAAATG
AAGCTGATCGGGGATCGGATGGGAATCGATATATACGAAGTCATCGATGCCGCAGCGACCAAGCCTTTTGGCTTTACGCC
TTACTATCCGGGGCCGGGGCTGGGTGGGCACTGCATCCCCATCGATCCCTTCTACCTGACCTGGAAGGCTCGCGAGTATG
GCGTGTCAACGCGCTTCATCGAACTGGCCGGTGAAATCAATCGCACCATGCCCGAGTGGGTGGTCTCGAAAGTGGCCGAA
GGCCTGAACGAGCGATGCAAGGCGCTCAAGGGCAGTCGCATTCTGGCACTCGGCATCGCCTACAAGCGCGATATCGAGGA
CTGCCGCGAATCGCCGTCGGTCGAAGTGATGGAAGTGTTGCGCGACCGCGGAGCGGTGGTCGAATATTCCGACCCCTATG
CGCCGGTCTTCCCGCGCATGCGGGAGCATAGTTTCGATCTGGAGTCGGTCGAACTCACACCCGAAACGATCGCTTCATAC
GACGCGGTTGTGCTACTGACGGATCACACCGACTTCGACTACGACCTCATCCTCGAAAAAGCGAAGCTTGTAATCGACAC
GCGTGGGAAATTCCGCGAGCCCAAACCCAATGTGGTGCGTGCATGA

Upstream 100 bases:

>100_bases
CATGCACCCCTACCTTTCCAGCGACGAAACCAGCCAGGTCGTTGACGCCGTGATCTCGGCCGTGCGCGCCTGATTTTACC
AGCTACCAGGACATTACATC

Downstream 100 bases:

>100_bases
CTACGAGGACGCGAGCGGGATAACTCGGTCGGTAGGGCCTCTCCTTTCCGCCATTGCGTGTCTTTGCACTTGTGGACCTA
CTTTCGCCGCGCCATGGTCG

Product: udpglucose 6-dehydrogenase

Products: UDP-glucuronate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTATLSSIARETIARFENKSAKIGILGLGYVGIPLALRFSEVGLTVLGFDVDRERVRELNAGESPIKHIPASEIEGMKRT
GFEATDDFSRIPEVDAIIICVPTPLSEQREPDLSYVVSTMESVKGHLREGQLLSLESTTWPGTTDEVLKPYIDARGLTVG
EDFFLVYSPEREDPGNPDFTTRTIPKVLGGVTETCSAVGMALYGTVIDKVVPVSSTQAAELTKLLENIHRSVNIGLVNEM
KLIGDRMGIDIYEVIDAAATKPFGFTPYYPGPGLGGHCIPIDPFYLTWKAREYGVSTRFIELAGEINRTMPEWVVSKVAE
GLNERCKALKGSRILALGIAYKRDIEDCRESPSVEVMEVLRDRGAVVEYSDPYAPVFPRMREHSFDLESVELTPETIASY
DAVVLLTDHTDFDYDLILEKAKLVIDTRGKFREPKPNVVRA

Sequences:

>Translated_441_residues
MTATLSSIARETIARFENKSAKIGILGLGYVGIPLALRFSEVGLTVLGFDVDRERVRELNAGESPIKHIPASEIEGMKRT
GFEATDDFSRIPEVDAIIICVPTPLSEQREPDLSYVVSTMESVKGHLREGQLLSLESTTWPGTTDEVLKPYIDARGLTVG
EDFFLVYSPEREDPGNPDFTTRTIPKVLGGVTETCSAVGMALYGTVIDKVVPVSSTQAAELTKLLENIHRSVNIGLVNEM
KLIGDRMGIDIYEVIDAAATKPFGFTPYYPGPGLGGHCIPIDPFYLTWKAREYGVSTRFIELAGEINRTMPEWVVSKVAE
GLNERCKALKGSRILALGIAYKRDIEDCRESPSVEVMEVLRDRGAVVEYSDPYAPVFPRMREHSFDLESVELTPETIASY
DAVVLLTDHTDFDYDLILEKAKLVIDTRGKFREPKPNVVRA
>Mature_440_residues
TATLSSIARETIARFENKSAKIGILGLGYVGIPLALRFSEVGLTVLGFDVDRERVRELNAGESPIKHIPASEIEGMKRTG
FEATDDFSRIPEVDAIIICVPTPLSEQREPDLSYVVSTMESVKGHLREGQLLSLESTTWPGTTDEVLKPYIDARGLTVGE
DFFLVYSPEREDPGNPDFTTRTIPKVLGGVTETCSAVGMALYGTVIDKVVPVSSTQAAELTKLLENIHRSVNIGLVNEMK
LIGDRMGIDIYEVIDAAATKPFGFTPYYPGPGLGGHCIPIDPFYLTWKAREYGVSTRFIELAGEINRTMPEWVVSKVAEG
LNERCKALKGSRILALGIAYKRDIEDCRESPSVEVMEVLRDRGAVVEYSDPYAPVFPRMREHSFDLESVELTPETIASYD
AVVLLTDHTDFDYDLILEKAKLVIDTRGKFREPKPNVVRA

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4507813, Length=382, Percent_Identity=25.6544502617801, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI48994968, Length=435, Percent_Identity=32.8735632183908, Blast_Score=202, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17560350, Length=458, Percent_Identity=23.1441048034935, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17136908, Length=371, Percent_Identity=23.7196765498652, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732 [H]

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N [H]

EC number: 1.1.1.22

Molecular weight: Translated: 48797; Mature: 48666

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATLSSIARETIARFENKSAKIGILGLGYVGIPLALRFSEVGLTVLGFDVDRERVRELN
CCCCHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHEEEEHHCCEEEEEECCCHHHHHHCC
AGESPIKHIPASEIEGMKRTGFEATDDFSRIPEVDAIIICVPTPLSEQREPDLSYVVSTM
CCCCHHHCCCHHHHCCHHHCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHH
ESVKGHLREGQLLSLESTTWPGTTDEVLKPYIDARGLTVGEDFFLVYSPEREDPGNPDFT
HHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEECCCEEEEECCCCCCCCCCCCC
TRTIPKVLGGVTETCSAVGMALYGTVIDKVVPVSSTQAAELTKLLENIHRSVNIGLVNEM
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHH
KLIGDRMGIDIYEVIDAAATKPFGFTPYYPGPGLGGHCIPIDPFYLTWKAREYGVSTRFI
HHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCEEEEEEHHHHCCCHHHH
ELAGEINRTMPEWVVSKVAEGLNERCKALKGSRILALGIAYKRDIEDCRESPSVEVMEVL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCCHHHHHHH
RDRGAVVEYSDPYAPVFPRMREHSFDLESVELTPETIASYDAVVLLTDHTDFDYDLILEK
HHCCCEEEECCCCCCCCHHHHHCCCCCCEEEECHHHHHCCCEEEEEECCCCCCHHHHHHH
AKLVIDTRGKFREPKPNVVRA
HHEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TATLSSIARETIARFENKSAKIGILGLGYVGIPLALRFSEVGLTVLGFDVDRERVRELN
CCCHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHEEEEHHCCEEEEEECCCHHHHHHCC
AGESPIKHIPASEIEGMKRTGFEATDDFSRIPEVDAIIICVPTPLSEQREPDLSYVVSTM
CCCCHHHCCCHHHHCCHHHCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHH
ESVKGHLREGQLLSLESTTWPGTTDEVLKPYIDARGLTVGEDFFLVYSPEREDPGNPDFT
HHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEECCCEEEEECCCCCCCCCCCCC
TRTIPKVLGGVTETCSAVGMALYGTVIDKVVPVSSTQAAELTKLLENIHRSVNIGLVNEM
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHH
KLIGDRMGIDIYEVIDAAATKPFGFTPYYPGPGLGGHCIPIDPFYLTWKAREYGVSTRFI
HHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCEEEEEEHHHHCCCHHHH
ELAGEINRTMPEWVVSKVAEGLNERCKALKGSRILALGIAYKRDIEDCRESPSVEVMEVL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCCHHHHHHH
RDRGAVVEYSDPYAPVFPRMREHSFDLESVELTPETIASYDAVVLLTDHTDFDYDLILEK
HHCCCEEEECCCCCCCCHHHHHCCCCCCEEEECHHHHHCCCEEEEEECCCCCCHHHHHHH
AKLVIDTRGKFREPKPNVVRA
HHEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose; NAD+; H2O

Specific reaction: UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH + 2 H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]