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The map label for this gene is rho

Identifier: 85375430

GI number: 85375430

Start: 2620134

End: 2621399

Strand: Direct

Name: rho

Synonym: ELI_13015

Alternate gene names: 85375430

Gene position: 2620134-2621399 (Clockwise)

Preceding gene: 85375429

Following gene: 85375431

Centisome position: 85.84

GC content: 60.19

Gene sequence:

>1266_bases
ATGCATCTCAGAGATCTCAAAGAGAAAACCCCGGCCGAGCTTGTCTCGATGGCCGAAGAACTGGGCGTCGAAGGCGCCAG
CACCATGCGCCGGCAGGACTTGCTGTTCAGCATCCTGCGCGAGCTTGCAGAGGACGAAGAATACGAAGAACAGATCATGG
GTATCGGCACCATCGAGGTGCTGCAGGACGGCTTCGGCTTCCTGCGTTCGCCCGAGGCGAACTATCTGGCCGGGCCGGAC
GATATCTATGTTTCCCCCAACCAGATCCGCAAATGGGGCCTGCGTACCGGTGACACCGTCGAAGGTGAAATCCGCGCGCC
CAACGAGGGCGAACGCTATTTCGCGCTGACCAGCCTGAAAACGGTCAATTTCGATGAGCCGGATGCCGTGCGCCGCCGGA
CCAATTTCGATAACCTCACGCCGCTCTATCCGGACCAGAAGCTGTCGCTCGATACGCTCGACCCGACGGTGAAGGACAAG
TCGGCCCGCGTGATCGACATCATTTCACCGCAGGGCAAGGGCCAGCGCGCGTTGATCGTCGCCCCGCCGCGTACGGGTAA
GACCGTACTGCTGCAGAATATCGCCAAGGCGATTACCGACAATCACCCTGAAGTCTTCCTGCTGGTTCTGCTCGTCGACG
AGCGGCCCGAGGAAGTCACCGACATGCAGCGCAGCGTAAAGGGCGAAGTCATTTCCTCGACCTTCGACGAGCCGGCCAAC
CGCCACGTCCAGGTCGCCGAGATGGTGATCGAGAAGGCCAAGCGCCTCGTCGAGCACAAGCACGATGTCGTCATCCTGCT
CGATTCGATCACGCGCCTCGGCCGTGCCTACAACACCGTGGTGCCGAGCTCGGGCAAGGTCCTGACCGGCGGTGTCGATG
CCAATGCGCTCCAGCGCCCGAAGCGCTTCTTCGGTGCCGCGCGTAACATCGAGGAAGGCGGCTCGCTCTCGATCATCGCC
ACCGCGCTGATCGATACCGGCAGTCGCATGGACGAGGTTATCTTCGAGGAATTCAAGGGCACGGGTAACAGCGAAATCGT
GCTCGACCGCAAGGTCGCCGACAAGCGCATCTTCCCAGCATTGGATGTCGGCAAGTCCGGCACCCGCAAGGAAGAGCTGC
TGGTCGAGAAGGACAAGCTCTCCAAGATGTGGGTCCTGCGCCGTATCCTCATGCAGATGGGCACGATCGACGCCATGGAA
TTCTTGCTCGACAAGATGAAGGATTCCAAGACCAACGAAGACTTCTTCGAGACGATGAACCAGTAA

Upstream 100 bases:

>100_bases
GCATCGTAAAGCTTTCCCCAAGCCTGTCCTGAGCCCAGTCGAAGGACCCGATGAGACCGGCCGGCTATTTTCCAATTCCT
TCCATTTCTGGAAACGACGA

Downstream 100 bases:

>100_bases
TCCGGGAACCAGCAAGGTGTCCGAACCCGCACCCCGCCGCATCGCTATTACGGGTGCGCCGGGGGCGGGAAAGTCCACTC
TGCTGCACGCGCTCGGGGAT

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MHLRDLKEKTPAELVSMAEELGVEGASTMRRQDLLFSILRELAEDEEYEEQIMGIGTIEVLQDGFGFLRSPEANYLAGPD
DIYVSPNQIRKWGLRTGDTVEGEIRAPNEGERYFALTSLKTVNFDEPDAVRRRTNFDNLTPLYPDQKLSLDTLDPTVKDK
SARVIDIISPQGKGQRALIVAPPRTGKTVLLQNIAKAITDNHPEVFLLVLLVDERPEEVTDMQRSVKGEVISSTFDEPAN
RHVQVAEMVIEKAKRLVEHKHDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLSIIA
TALIDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPALDVGKSGTRKEELLVEKDKLSKMWVLRRILMQMGTIDAME
FLLDKMKDSKTNEDFFETMNQ

Sequences:

>Translated_421_residues
MHLRDLKEKTPAELVSMAEELGVEGASTMRRQDLLFSILRELAEDEEYEEQIMGIGTIEVLQDGFGFLRSPEANYLAGPD
DIYVSPNQIRKWGLRTGDTVEGEIRAPNEGERYFALTSLKTVNFDEPDAVRRRTNFDNLTPLYPDQKLSLDTLDPTVKDK
SARVIDIISPQGKGQRALIVAPPRTGKTVLLQNIAKAITDNHPEVFLLVLLVDERPEEVTDMQRSVKGEVISSTFDEPAN
RHVQVAEMVIEKAKRLVEHKHDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLSIIA
TALIDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPALDVGKSGTRKEELLVEKDKLSKMWVLRRILMQMGTIDAME
FLLDKMKDSKTNEDFFETMNQ
>Mature_421_residues
MHLRDLKEKTPAELVSMAEELGVEGASTMRRQDLLFSILRELAEDEEYEEQIMGIGTIEVLQDGFGFLRSPEANYLAGPD
DIYVSPNQIRKWGLRTGDTVEGEIRAPNEGERYFALTSLKTVNFDEPDAVRRRTNFDNLTPLYPDQKLSLDTLDPTVKDK
SARVIDIISPQGKGQRALIVAPPRTGKTVLLQNIAKAITDNHPEVFLLVLLVDERPEEVTDMQRSVKGEVISSTFDEPAN
RHVQVAEMVIEKAKRLVEHKHDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLSIIA
TALIDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPALDVGKSGTRKEELLVEKDKLSKMWVLRRILMQMGTIDAME
FLLDKMKDSKTNEDFFETMNQ

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=421, Percent_Identity=65.083135391924, Blast_Score=582, Evalue=1e-167,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 47247; Mature: 47247

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLRDLKEKTPAELVSMAEELGVEGASTMRRQDLLFSILRELAEDEEYEEQIMGIGTIEV
CCCHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHH
LQDGFGFLRSPEANYLAGPDDIYVSPNQIRKWGLRTGDTVEGEIRAPNEGERYFALTSLK
HHHHHHHHCCCCCCCCCCCCCEEECHHHHHHCCCCCCCCCCCEEECCCCCCEEEEEEEEE
TVNFDEPDAVRRRTNFDNLTPLYPDQKLSLDTLDPTVKDKSARVIDIISPQGKGQRALIV
ECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEE
APPRTGKTVLLQNIAKAITDNHPEVFLLVLLVDERPEEVTDMQRSVKGEVISSTFDEPAN
ECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHC
RHVQVAEMVIEKAKRLVEHKHDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRP
CHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHH
KRFFGAARNIEEGGSLSIIATALIDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPA
HHHHHHHCCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECHHHCCCCCCC
LDVGKSGTRKEELLVEKDKLSKMWVLRRILMQMGTIDAMEFLLDKMKDSKTNEDFFETMN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHC
Q
C
>Mature Secondary Structure
MHLRDLKEKTPAELVSMAEELGVEGASTMRRQDLLFSILRELAEDEEYEEQIMGIGTIEV
CCCHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHH
LQDGFGFLRSPEANYLAGPDDIYVSPNQIRKWGLRTGDTVEGEIRAPNEGERYFALTSLK
HHHHHHHHCCCCCCCCCCCCCEEECHHHHHHCCCCCCCCCCCEEECCCCCCEEEEEEEEE
TVNFDEPDAVRRRTNFDNLTPLYPDQKLSLDTLDPTVKDKSARVIDIISPQGKGQRALIV
ECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEE
APPRTGKTVLLQNIAKAITDNHPEVFLLVLLVDERPEEVTDMQRSVKGEVISSTFDEPAN
ECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHC
RHVQVAEMVIEKAKRLVEHKHDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRP
CHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHH
KRFFGAARNIEEGGSLSIIATALIDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPA
HHHHHHHCCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECHHHCCCCCCC
LDVGKSGTRKEELLVEKDKLSKMWVLRRILMQMGTIDAMEFLLDKMKDSKTNEDFFETMN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606169 [H]