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The map label for this gene is gsk [C]

Identifier: 85375371

GI number: 85375371

Start: 2571972

End: 2572967

Strand: Reverse

Name: gsk [C]

Synonym: ELI_12720

Alternate gene names: 85375371

Gene position: 2572967-2571972 (Counterclockwise)

Preceding gene: 85375375

Following gene: 85375369

Centisome position: 84.29

GC content: 65.56

Gene sequence:

>996_bases
ATGACTGAGCCCCGCTACGACGTCATCGCCATCGGCAATGCCGTGGTCGATGTGATCGCTTCCTGCGAAGACGAGCTGAT
CGACGAGCTCGACCTCAATCGCGGCGGGATGACACTGATCGACGAAGCGCGCGCGAAGGAACTGTACGACGCGATGCCGC
CGGCGCGCGAAGTCTCGGGCGGTTCGGCGGCCAATACGCTCGCTGGCCTCTCGACGCTTGGCCTCCAATGCGCCTTCATC
GGCCAGGTCGCCGACGACCAGCTGGGCGAAGTCTTCCGCCACGACATGCGCGCCACCGGCATCGATTTCGACACGCCTGC
GCGCGAAGGCGAGCCCGCCACAGGCCGCGTGATGATCTTCGTGACGCCCGATGGCGAGCGGACGATGAACACCTTTCTCG
GCGCGGGACAATTCCTGCCCGCCGAAGCGCTCGACGAGGAATTAATCGCCAGCGGCGGCATCCTCTATCTCGAAGGTTAT
CTCTGGGATCCGGAAGAGCCGCGCAAGGCCATGCGCCGCGCGATCGAAGTGGCGCGCTCTGCCGGGCGCAAGATCGCCTT
CACTGCCAGCGAAAGCTTCGTGATCGACCGCCACGGTGACGATTTCCGCGCCATGATCGACGACGGCGTAATCGACATCC
TGTTCGTCAACGAGCATGAGCTCGCGACGCTGACGGGCGAAGAAGATTTCGACAAGGGCGTCGCCATGGTCGCGCCCAAG
GTGCCCGTGCTGGTCGCCACCCGCAGCGAAAGAGGCGCTATCGCGGTCGCTAACGGCACCCGCGCCGAAGTCGCCGCCGA
GCCGATCGACAAGGTCGTCGACACCACCGGCGCCGGCGACCAATTCGCCGCCGGCTTCCTGTCGGGCCATGCGAGGGGCG
AAGACCTGCAGGCCTGCCTCAAGCGCGGCGCAATCGCAGCGGCGGAAGTGATCAGCCACTACGGCCCGCGCCCCGAGGTC
GATATGAAAGCGCTGATGGCTCAGAAGCTGAGCTAG

Upstream 100 bases:

>100_bases
GCCATTGTCGCGCGGCGCGCAAGACAGGGCTTGGCCGGTGCATGGCAAGCGGCTAGGCGCGGCGCGAGTTTCTTGCCCGC
TGCAATCGACGGAGTTTTCC

Downstream 100 bases:

>100_bases
GCTTTCCGCTCCCGCTTGATCTCGCGCCAGCCGATATCGCGGCGGCAGAAGCCGGTGGGGAAGTCGATCGCGTCGACAGC
CTGGTAGGCGGCGCGCTGGG

Product: sugar kinase

Products: ADP; H+; IMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTEPRYDVIAIGNAVVDVIASCEDELIDELDLNRGGMTLIDEARAKELYDAMPPAREVSGGSAANTLAGLSTLGLQCAFI
GQVADDQLGEVFRHDMRATGIDFDTPAREGEPATGRVMIFVTPDGERTMNTFLGAGQFLPAEALDEELIASGGILYLEGY
LWDPEEPRKAMRRAIEVARSAGRKIAFTASESFVIDRHGDDFRAMIDDGVIDILFVNEHELATLTGEEDFDKGVAMVAPK
VPVLVATRSERGAIAVANGTRAEVAAEPIDKVVDTTGAGDQFAAGFLSGHARGEDLQACLKRGAIAAAEVISHYGPRPEV
DMKALMAQKLS

Sequences:

>Translated_331_residues
MTEPRYDVIAIGNAVVDVIASCEDELIDELDLNRGGMTLIDEARAKELYDAMPPAREVSGGSAANTLAGLSTLGLQCAFI
GQVADDQLGEVFRHDMRATGIDFDTPAREGEPATGRVMIFVTPDGERTMNTFLGAGQFLPAEALDEELIASGGILYLEGY
LWDPEEPRKAMRRAIEVARSAGRKIAFTASESFVIDRHGDDFRAMIDDGVIDILFVNEHELATLTGEEDFDKGVAMVAPK
VPVLVATRSERGAIAVANGTRAEVAAEPIDKVVDTTGAGDQFAAGFLSGHARGEDLQACLKRGAIAAAEVISHYGPRPEV
DMKALMAQKLS
>Mature_330_residues
TEPRYDVIAIGNAVVDVIASCEDELIDELDLNRGGMTLIDEARAKELYDAMPPAREVSGGSAANTLAGLSTLGLQCAFIG
QVADDQLGEVFRHDMRATGIDFDTPAREGEPATGRVMIFVTPDGERTMNTFLGAGQFLPAEALDEELIASGGILYLEGYL
WDPEEPRKAMRRAIEVARSAGRKIAFTASESFVIDRHGDDFRAMIDDGVIDILFVNEHELATLTGEEDFDKGVAMVAPKV
PVLVATRSERGAIAVANGTRAEVAAEPIDKVVDTTGAGDQFAAGFLSGHARGEDLQACLKRGAIAAAEVISHYGPRPEVD
MKALMAQKLS

Specific function: Unknown

COG id: COG0524

COG function: function code G; Sugar kinases, ribokinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Homo sapiens, GI32484973, Length=343, Percent_Identity=25.3644314868805, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI32484975, Length=335, Percent_Identity=25.0746268656716, Blast_Score=89, Evalue=8e-18,
Organism=Escherichia coli, GI1786684, Length=315, Percent_Identity=26.984126984127, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081971, Length=318, Percent_Identity=25.1572327044025, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17541820, Length=335, Percent_Identity=22.6865671641791, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17532905, Length=269, Percent_Identity=27.5092936802974, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322565, Length=340, Percent_Identity=24.1176470588235, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24663633, Length=332, Percent_Identity=21.9879518072289, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21356339, Length=332, Percent_Identity=21.9879518072289, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: 2.7.1.73 [C]

Molecular weight: Translated: 35384; Mature: 35253

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPRYDVIAIGNAVVDVIASCEDELIDELDLNRGGMTLIDEARAKELYDAMPPAREVSG
CCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCCCHHHCCC
GSAANTLAGLSTLGLQCAFIGQVADDQLGEVFRHDMRATGIDFDTPAREGEPATGRVMIF
CCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEE
VTPDGERTMNTFLGAGQFLPAEALDEELIASGGILYLEGYLWDPEEPRKAMRRAIEVARS
ECCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHH
AGRKIAFTASESFVIDRHGDDFRAMIDDGVIDILFVNEHELATLTGEEDFDKGVAMVAPK
CCCEEEEECCCCEEEECCCCHHHHHHCCCCEEEEEECCCCEEEECCCCCHHCCEEEECCC
VPVLVATRSERGAIAVANGTRAEVAAEPIDKVVDTTGAGDQFAAGFLSGHARGEDLQACL
CCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHH
KRGAIAAAEVISHYGPRPEVDMKALMAQKLS
HHCCHHHHHHHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TEPRYDVIAIGNAVVDVIASCEDELIDELDLNRGGMTLIDEARAKELYDAMPPAREVSG
CCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCCCHHHCCC
GSAANTLAGLSTLGLQCAFIGQVADDQLGEVFRHDMRATGIDFDTPAREGEPATGRVMIF
CCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEE
VTPDGERTMNTFLGAGQFLPAEALDEELIASGGILYLEGYLWDPEEPRKAMRRAIEVARS
ECCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHH
AGRKIAFTASESFVIDRHGDDFRAMIDDGVIDILFVNEHELATLTGEEDFDKGVAMVAPK
CCCEEEEECCCCEEEECCCCHHHHHHCCCCEEEEEECCCCEEEECCCCCHHCCEEEECCC
VPVLVATRSERGAIAVANGTRAEVAAEPIDKVVDTTGAGDQFAAGFLSGHARGEDLQACL
CCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHH
KRGAIAAAEVISHYGPRPEVDMKALMAQKLS
HHCCHHHHHHHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; Inosine [C]

Specific reaction: ATP + Inosine --> ADP + H+ + IMP [C]

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]