Click here to switch to the map view.

The map label for this gene is wcaI [H]

Identifier: 85375276

GI number: 85375276

Start: 2475514

End: 2476728

Strand: Reverse

Name: wcaI [H]

Synonym: ELI_12245

Alternate gene names: 85375276

Gene position: 2476728-2475514 (Counterclockwise)

Preceding gene: 85375278

Following gene: 85375275

Centisome position: 81.14

GC content: 66.5

Gene sequence:

>1215_bases
ATGATCAGCCTGAATTACGCGCCGGAGCCGATCGGCATCGGTCCCTACAGCCAGGGATGGGCTGAGCATCTCGCCGGGCG
CGGTCACGAGGTGCGCGTGATTTGCGGCGTCCCCTATTATCCGGACTGGCAGCTGGCAGCGGGTTTCGCGCAGGAATTCA
GCGACAGTTGCGAAAGGGGTGTGCAGGTAAAGAGGGTCCCTCACCATCTTCCGGCACGGCCCAATACAATTGGGCGCTTG
CGGCACTATCTTTCGTTTGCGAGCAATGTTCGCAAGGTCATCCGGCGAGGCACCGCCGATTTCGAGCCCGAGCTTGTCAT
TTCCATTGCCCCGTCGCTTCTGGCGGCGCCCGTCGCCATCGGCGCAGCGCGTAGGGCCGGCGCAGTGACATGGCTGCATA
TTCAGGATTTCGAGGTCGAAGCAGCGGTGGCGACAGGCCTGCTGCCCGGCCCGGTCGCCAGGCTTGCTGCACGCTTCGAA
GCCTCGATCATCAGCCGCTTCGATCGGGTCAGCACTATTTCGCCGAAGATGGTCGCGAAGGCCGAACGGGAGAAGGGCGC
CGCGGCTCCCGTCGCGGAGTTGCGCAACTGGGCCGAGCCCGAGGTCGGCGGCGACGCGGCAAACGGCGATGCGATGCGTG
CGAAACTCGACCTGCCGCCGGGCCTGATCGCGCTCTATTCCGGCAATCTCGCGCGCAAGCAGGGGGTCGGACTCATCCTC
GAAGCGGCCAGAGCCATGCAGGCAAGGCCCGACCTCCAGTTCGTGGTCTGCGGCGAGGGGCCTGCCGCAGGGGAGATCGC
GGCCGCCGCCGCGCATCTGCCCAATCTCCATCTCCGCCCCCTGCAGCCGCGTGATCGCTTGCCCGACCTTCTCGCCATGG
CCGATCTCCACTTGCTGCCGCAGATCGCCGGCGCGGCGGACCTTGTGCTTCCGTCCAAGCTGGCGAACATGCTCGCGTCC
GGGCGGCCGGTAGTGGCGACGAGCGATCCCGGCACCTCGCTGGCGCATGAAGTCGAGGGGTGCGGGTTGTGCACGCCGGC
CGGCGATACGGAATCCTTCGCGCGCGCCATCGAGGCGTTGGCTTCAGACGCCTCTATGCGGCGCTCCTTGGGCCGCGAAG
CGGCACAACGCGCAACCGAGCGCTGGTCAAGACAGGCGTTGCTTGCCAAGATGGCGGACGAGATCGAAAGGGCGATTGCG
GAGCGCGGCGAATGA

Upstream 100 bases:

>100_bases
AAAGTATTCGGAGGACGATTTCGATGCGCGTTGCGGATCTTCGCAGCGCGGGCAAACGGTGGGCGAACATAGGCGCGAAG
CAATCATGGCCCGTATCTGC

Downstream 100 bases:

>100_bases
GTTACGTGCCGAAAGGGACCGCAGCGATCGCCCGCCCGCGTCCCGCGCCACCCATTCGCCGCGCCGCCGCGCATAGCGAG
GTCAGGTTCGATCCCTGGAT

Product: putative glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MISLNYAPEPIGIGPYSQGWAEHLAGRGHEVRVICGVPYYPDWQLAAGFAQEFSDSCERGVQVKRVPHHLPARPNTIGRL
RHYLSFASNVRKVIRRGTADFEPELVISIAPSLLAAPVAIGAARRAGAVTWLHIQDFEVEAAVATGLLPGPVARLAARFE
ASIISRFDRVSTISPKMVAKAEREKGAAAPVAELRNWAEPEVGGDAANGDAMRAKLDLPPGLIALYSGNLARKQGVGLIL
EAARAMQARPDLQFVVCGEGPAAGEIAAAAAHLPNLHLRPLQPRDRLPDLLAMADLHLLPQIAGAADLVLPSKLANMLAS
GRPVVATSDPGTSLAHEVEGCGLCTPAGDTESFARAIEALASDASMRRSLGREAAQRATERWSRQALLAKMADEIERAIA
ERGE

Sequences:

>Translated_404_residues
MISLNYAPEPIGIGPYSQGWAEHLAGRGHEVRVICGVPYYPDWQLAAGFAQEFSDSCERGVQVKRVPHHLPARPNTIGRL
RHYLSFASNVRKVIRRGTADFEPELVISIAPSLLAAPVAIGAARRAGAVTWLHIQDFEVEAAVATGLLPGPVARLAARFE
ASIISRFDRVSTISPKMVAKAEREKGAAAPVAELRNWAEPEVGGDAANGDAMRAKLDLPPGLIALYSGNLARKQGVGLIL
EAARAMQARPDLQFVVCGEGPAAGEIAAAAAHLPNLHLRPLQPRDRLPDLLAMADLHLLPQIAGAADLVLPSKLANMLAS
GRPVVATSDPGTSLAHEVEGCGLCTPAGDTESFARAIEALASDASMRRSLGREAAQRATERWSRQALLAKMADEIERAIA
ERGE
>Mature_404_residues
MISLNYAPEPIGIGPYSQGWAEHLAGRGHEVRVICGVPYYPDWQLAAGFAQEFSDSCERGVQVKRVPHHLPARPNTIGRL
RHYLSFASNVRKVIRRGTADFEPELVISIAPSLLAAPVAIGAARRAGAVTWLHIQDFEVEAAVATGLLPGPVARLAARFE
ASIISRFDRVSTISPKMVAKAEREKGAAAPVAELRNWAEPEVGGDAANGDAMRAKLDLPPGLIALYSGNLARKQGVGLIL
EAARAMQARPDLQFVVCGEGPAAGEIAAAAAHLPNLHLRPLQPRDRLPDLLAMADLHLLPQIAGAADLVLPSKLANMLAS
GRPVVATSDPGTSLAHEVEGCGLCTPAGDTESFARAIEALASDASMRRSLGREAAQRATERWSRQALLAKMADEIERAIA
ERGE

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788363, Length=393, Percent_Identity=38.4223918575064, Blast_Score=209, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 42999; Mature: 42999

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISLNYAPEPIGIGPYSQGWAEHLAGRGHEVRVICGVPYYPDWQLAAGFAQEFSDSCERG
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHC
VQVKRVPHHLPARPNTIGRLRHYLSFASNVRKVIRRGTADFEPELVISIAPSLLAAPVAI
CEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHH
GAARRAGAVTWLHIQDFEVEAAVATGLLPGPVARLAARFEASIISRFDRVSTISPKMVAK
HHHHHCCCEEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AEREKGAAAPVAELRNWAEPEVGGDAANGDAMRAKLDLPPGLIALYSGNLARKQGVGLIL
HHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCHHHHHCCHHHH
EAARAMQARPDLQFVVCGEGPAAGEIAAAAAHLPNLHLRPLQPRDRLPDLLAMADLHLLP
HHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QIAGAADLVLPSKLANMLASGRPVVATSDPGTSLAHEVEGCGLCTPAGDTESFARAIEAL
HHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHH
ASDASMRRSLGREAAQRATERWSRQALLAKMADEIERAIAERGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MISLNYAPEPIGIGPYSQGWAEHLAGRGHEVRVICGVPYYPDWQLAAGFAQEFSDSCERG
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHC
VQVKRVPHHLPARPNTIGRLRHYLSFASNVRKVIRRGTADFEPELVISIAPSLLAAPVAI
CEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHH
GAARRAGAVTWLHIQDFEVEAAVATGLLPGPVARLAARFEASIISRFDRVSTISPKMVAK
HHHHHCCCEEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AEREKGAAAPVAELRNWAEPEVGGDAANGDAMRAKLDLPPGLIALYSGNLARKQGVGLIL
HHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCHHHHHCCHHHH
EAARAMQARPDLQFVVCGEGPAAGEIAAAAAHLPNLHLRPLQPRDRLPDLLAMADLHLLP
HHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QIAGAADLVLPSKLANMLASGRPVVATSDPGTSLAHEVEGCGLCTPAGDTESFARAIEAL
HHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHH
ASDASMRRSLGREAAQRATERWSRQALLAKMADEIERAIAERGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503 [H]