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The map label for this gene is wcaF [H]

Identifier: 85375268

GI number: 85375268

Start: 2467974

End: 2468549

Strand: Direct

Name: wcaF [H]

Synonym: ELI_12205

Alternate gene names: 85375268

Gene position: 2467974-2468549 (Clockwise)

Preceding gene: 85375267

Following gene: 85375270

Centisome position: 80.85

GC content: 67.19

Gene sequence:

>576_bases
GTGACCGAGCCCCTCGATGCCGAAGCCCGTGACACGCTCGGCGGAGGCCCGAGCTTCTCAGTCGCCAATCGCCTCGAACG
TGCGCTTTTCGCCGTGTGCTGGACGCTGTTTGCGTGGGTCCTGCCGCCGCCTTTGGGGTGGGGCTGGCGACGCTTTCTCC
TCCGCCTCTTCGGGGCCCGGATCGAAGCGAAGGCAAAGGTCTATCCCAGCGCGAAAATCTGGTTGCCGCGACATTTGCGG
ATGGCGGCAGGCTCGACGCTCGGGCCCTCGGCCAATTGCTACAACATGGCGCCGATCTCGCTCGGTGCCGGTGCCATTGT
ATCGCAACGCGCCTTCCTGTGCGCGGGCGACCACGATTTCCGGAATCCGAGCTTCCAGCTTGTCGCCCGACCGATCGTCC
TTGAACCGCAAAGCTGGGTCGCGGCGGAGGCTTTCGTCGGGCCCGGTGTGACATTGGCCGAAGGATCGGTGCTGGCTGCC
CGGGGGGTCGCCGCGAAGGACCTCCCCGCATGGACCATCTGGGGCGGCAATCCCGCAGCGCAAATTGGGACGCGCGACCG
GCGGGCGACCCGCTAG

Upstream 100 bases:

>100_bases
CAAGGGATTGCAGCCAGCCGATCCTGCTCCTTGCCGCAGACGGGAAGGGCGCACGGGGTGGAGCCGCACGGCTGGAACGC
GTAGCCGTCGAGCCGGTATC

Downstream 100 bases:

>100_bases
GCCGCAGTCAGCCGGTCGAACAGAGCATTGGCCTCAGCCAGTGTCCTCGCATCGAAAGCAAGGTCGAGCGCATTGCGGTG
AACCAGCTTGTCTTCGCTGG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 191; Mature: 190

Protein sequence:

>191_residues
MTEPLDAEARDTLGGGPSFSVANRLERALFAVCWTLFAWVLPPPLGWGWRRFLLRLFGARIEAKAKVYPSAKIWLPRHLR
MAAGSTLGPSANCYNMAPISLGAGAIVSQRAFLCAGDHDFRNPSFQLVARPIVLEPQSWVAAEAFVGPGVTLAEGSVLAA
RGVAAKDLPAWTIWGGNPAAQIGTRDRRATR

Sequences:

>Translated_191_residues
MTEPLDAEARDTLGGGPSFSVANRLERALFAVCWTLFAWVLPPPLGWGWRRFLLRLFGARIEAKAKVYPSAKIWLPRHLR
MAAGSTLGPSANCYNMAPISLGAGAIVSQRAFLCAGDHDFRNPSFQLVARPIVLEPQSWVAAEAFVGPGVTLAEGSVLAA
RGVAAKDLPAWTIWGGNPAAQIGTRDRRATR
>Mature_190_residues
TEPLDAEARDTLGGGPSFSVANRLERALFAVCWTLFAWVLPPPLGWGWRRFLLRLFGARIEAKAKVYPSAKIWLPRHLRM
AAGSTLGPSANCYNMAPISLGAGAIVSQRAFLCAGDHDFRNPSFQLVARPIVLEPQSWVAAEAFVGPGVTLAEGSVLAAR
GVAAKDLPAWTIWGGNPAAQIGTRDRRATR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1788367, Length=161, Percent_Identity=40.9937888198758, Blast_Score=125, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.- [C]

Molecular weight: Translated: 20593; Mature: 20462

Theoretical pI: Translated: 10.81; Mature: 10.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPLDAEARDTLGGGPSFSVANRLERALFAVCWTLFAWVLPPPLGWGWRRFLLRLFGAR
CCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
IEAKAKVYPSAKIWLPRHLRMAAGSTLGPSANCYNMAPISLGAGAIVSQRAFLCAGDHDF
CCCCEEECCCEEEECCHHHHHHCCCCCCCCCCCEEECCCCCCCCHHHHCCEEEEECCCCC
RNPSFQLVARPIVLEPQSWVAAEAFVGPGVTLAEGSVLAARGVAAKDLPAWTIWGGNPAA
CCCCCEEEECCEEECCCCHHHHHHHCCCCEEEECCCEEEECCCCCCCCCEEEEECCCCHH
QIGTRDRRATR
HCCCCCCCCCC
>Mature Secondary Structure 
TEPLDAEARDTLGGGPSFSVANRLERALFAVCWTLFAWVLPPPLGWGWRRFLLRLFGAR
CCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
IEAKAKVYPSAKIWLPRHLRMAAGSTLGPSANCYNMAPISLGAGAIVSQRAFLCAGDHDF
CCCCEEECCCEEEECCHHHHHHCCCCCCCCCCCEEECCCCCCCCHHHHCCEEEEECCCCC
RNPSFQLVARPIVLEPQSWVAAEAFVGPGVTLAEGSVLAARGVAAKDLPAWTIWGGNPAA
CCCCCEEEECCEEECCCCHHHHHHHCCCCEEEECCCEEEECCCCCCCCCEEEEECCCCHH
QIGTRDRRATR
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]