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The map label for this gene is polA [H]

Identifier: 85375259

GI number: 85375259

Start: 2457977

End: 2458597

Strand: Direct

Name: polA [H]

Synonym: ELI_12160

Alternate gene names: 85375259

Gene position: 2457977-2458597 (Clockwise)

Preceding gene: 85375254

Following gene: 85375260

Centisome position: 80.53

GC content: 63.77

Gene sequence:

>621_bases
ATGGCTGTACATTTTCATGAAGAAGACCTGCCCGCGGGCGTGCTCGCAGGCGATGCGCCGCTGGCGGTCGATACCGAAAC
CATGGGGCTCGTCACCCGGCGCGACCGGCTCTGCATCGTCCAGATCAGCGATGGCGGAGAGGACGAGCATCTGGTCCGCT
TCGCCCCCGACAGCGACTATGCCGCGCCCAATCTGAAGGCACTGCTGGCCGATACGAACCGCGTGAAGCTCTATCACTTC
GCACGCTTCGACCTTGCCGCGATCGAGCACTATCTCGGCGTCACCGCCGCACCGGTCTTCTGCACCAAGATCGCCAGCAA
ACTGACCCGCACCTATACCGACCGCCATGGCCTCAAGAACCTGGTCGAGGAATTGCTCGGCGAGAACCTCTCCAAGCAGC
AACAGTCGAGCGACTGGGGCGGGGCCGACATCAACGAGGCGCAGCGCGATTATGCCGCGAGCGACGTCCGCTACCTCCAT
CGCATGCGCGAGATCCTGATCACCCGCCTCGAGCGCGAGGGCCGGACGGAGATGGCGCAGGCCTGTTTCGATTTCCTCCC
CACCCGCGCGCGGCTCGACCTTGCCGGATGGGAAGAGCACGACATCTTCCACCACAGTTAA

Upstream 100 bases:

>100_bases
CGGGGACGGTACCGGCCATGGGCCGAGACTACAGCGTCGCAAAACGCCTCGCCATGTCCCCTTTCCCTCTTTCCCCAATC
GGCCTAGAGCGCTTGGCTCT

Downstream 100 bases:

>100_bases
TACAAGGCGCGATACACCCACGCCATGGTCAGCCACCGCCGGATCGAAACGCAAGACGCGAAGGAACTGCGCAAGGGGCG
CCAGGCCTTCGCTGCGCCCG

Product: ribonuclease D 3'-5' exonuclease

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MAVHFHEEDLPAGVLAGDAPLAVDTETMGLVTRRDRLCIVQISDGGEDEHLVRFAPDSDYAAPNLKALLADTNRVKLYHF
ARFDLAAIEHYLGVTAAPVFCTKIASKLTRTYTDRHGLKNLVEELLGENLSKQQQSSDWGGADINEAQRDYAASDVRYLH
RMREILITRLEREGRTEMAQACFDFLPTRARLDLAGWEEHDIFHHS

Sequences:

>Translated_206_residues
MAVHFHEEDLPAGVLAGDAPLAVDTETMGLVTRRDRLCIVQISDGGEDEHLVRFAPDSDYAAPNLKALLADTNRVKLYHF
ARFDLAAIEHYLGVTAAPVFCTKIASKLTRTYTDRHGLKNLVEELLGENLSKQQQSSDWGGADINEAQRDYAASDVRYLH
RMREILITRLEREGRTEMAQACFDFLPTRARLDLAGWEEHDIFHHS
>Mature_205_residues
AVHFHEEDLPAGVLAGDAPLAVDTETMGLVTRRDRLCIVQISDGGEDEHLVRFAPDSDYAAPNLKALLADTNRVKLYHFA
RFDLAAIEHYLGVTAAPVFCTKIASKLTRTYTDRHGLKNLVEELLGENLSKQQQSSDWGGADINEAQRDYAASDVRYLHR
MREILITRLEREGRTEMAQACFDFLPTRARLDLAGWEEHDIFHHS

Specific function: Cleaves Multimeric tRNA Precursor At The Spacer Region. [C]

COG id: COG0349

COG function: function code J; Ribonuclease D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 3'-5' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1788105, Length=155, Percent_Identity=31.6129032258064, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR002298
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 23248; Mature: 23117

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVHFHEEDLPAGVLAGDAPLAVDTETMGLVTRRDRLCIVQISDGGEDEHLVRFAPDSDY
CEEECCCCCCCCCEEECCCCCEECCHHHHHEEECCCEEEEEECCCCCCCCEEEECCCCCC
AAPNLKALLADTNRVKLYHFARFDLAAIEHYLGVTAAPVFCTKIASKLTRTYTDRHGLKN
CCCCHHEEEECCCEEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVEELLGENLSKQQQSSDWGGADINEAQRDYAASDVRYLHRMREILITRLEREGRTEMAQ
HHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ACFDFLPTRARLDLAGWEEHDIFHHS
HHHHHCCCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure 
AVHFHEEDLPAGVLAGDAPLAVDTETMGLVTRRDRLCIVQISDGGEDEHLVRFAPDSDY
EEECCCCCCCCCEEECCCCCEECCHHHHHEEECCCEEEEEECCCCCCCCEEEECCCCCC
AAPNLKALLADTNRVKLYHFARFDLAAIEHYLGVTAAPVFCTKIASKLTRTYTDRHGLKN
CCCCHHEEEECCCEEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVEELLGENLSKQQQSSDWGGADINEAQRDYAASDVRYLHRMREILITRLEREGRTEMAQ
HHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ACFDFLPTRARLDLAGWEEHDIFHHS
HHHHHCCCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]