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The map label for this gene is gltB [H]

Identifier: 85375253

GI number: 85375253

Start: 2452787

End: 2454223

Strand: Direct

Name: gltB [H]

Synonym: ELI_12130

Alternate gene names: 85375253

Gene position: 2452787-2454223 (Clockwise)

Preceding gene: 85375252

Following gene: 85375254

Centisome position: 80.36

GC content: 64.58

Gene sequence:

>1437_bases
GTGGGCAAGGAAACAGGCTTTCTCGAACTCGACCGGCGCGAGCGCGATTATATCGCGCCCGAAGAACGTCTGAAAAGCTG
GCGCGAATTCATCAAGGAACCGCCGGTCGAAGAGCTCGCCGCGCAGGCCAGCCGCTGCATGAATTGCGGCATTCCGTATT
GTCACAACGGCTGCCCGGTGAACAACATCATCCCGGACTGGAACCACCTGGTCTATGAAGACGACTGGCAGAATGCGCTG
ACGGTGCTGCATTCGACCAACAATTTCCCCGAATTCACCGGCCGCATCTGCCCCGCCCCGTGCGAGGCGGCGTGCACGCT
CAACATCGTCGACCAGCCGGTGACGATCAAGTCGATCGAGGCGGCCATCATCGACCGCGGCTGGCGCGAAGGCTGGGTCA
AGCCCGAACCGCCCGAAAACGAAACCGGCAAGTCGGTCGCCGTGATCGGCAGCGGCCCAGCCGGGCTCGCCTGCGCGCAG
CAATTGGCGCGCGCGGGCCACGCGGTGACGGTGTTCGAGAAGCAGGACCGGATCGGCGGCCTGCTGCGCTACGGCATTCC
CGACTTCAAGATGGAGAAACACCTGATCAACCGGCGCGCCGTGCAGATGGAGACCGAAGGCGTGACCTTCAAAACCAGCG
CAGAAGTCGGCGTAGAAGTCAGCTTCCAGGCGCTGCGCGAGAATTTCGATGCGATCGTGCTGTCGGGCGGGGCGGAAGAG
CCGCGCCCGCTCGATATTCCGGGCGCGGAAATGTCCGGCGTTCGCTTCGCCATGGAATTCCTGACCCAGCAGAACAAACG
CAATGCCGGCGACGACGAAGTCCGCGCCGCCCCGCGCGGGACGCTGTCGGCCAAGGACAAGCATGTGATCGTGATCGGCG
GCGGCGATACCGGCAGCGACTGCGTCGGCACCTCCAACCGGCAGGGCGCCAAGAGCGTTACCCAGCTCGAAATCATGCCC
AAGCCGCCGGAGAAGGAAGACAAGGCGCTGACTTGGCCCGACTGGCCGCTCAAGCTGCGCACCAGTTCCAGCCACGAGGA
AGGCGTCGAGCGTGACTGGTCGGTCTTGACCAAGCGCGTAGTCGGCGACGGCGAAACCATGACCGGGCTCGAATGCGTCA
GGATCGAGTGGCGTGATGGCCAGATCGAGGAAATCGAAGGCAGCGAGTTCACCCTGCCCGCGGACCTTATCCTGCTCGCC
ATGGGCTTCACCGGTCCGAGGAAACGCGGCCTTCTCGACAAGGCCGGCGTCACCATGACCGAGCGCGGCAATGTCGAGGC
CAACGAGCAGGACTACGCCACCAGCGAACCGAACGTCTTCGCCTGCGGCGACATGCGGCGCGGGCAAAGCCTGGTCGTCT
GGGCGATCCGCGAAGGGCGCCAGTGCGCGCGCGCGGTGGACGAAGCATTGATGGGCGTGTCCGAACTTCCCCGCTAA

Upstream 100 bases:

>100_bases
AAATCGAACGTCATCCCAGCGCACGCTGGGATCTCTCTCAATCTGGCGCTTGGCCAGCAACAGATCCCAGCTTTCGCTGG
GATGACGAAAGTAGGGTAAC

Downstream 100 bases:

>100_bases
CCGATGCGCTGAACTGGCGACCATAGTCTTCTTGGGTAACACAGCGATACAGCAGTAATCGATCGGTTCTAATCGGATGA
TTGTCCGGTTTATCGGCTGG

Product: glutamate synthase, NADH/NADPH small subunit

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MGKETGFLELDRRERDYIAPEERLKSWREFIKEPPVEELAAQASRCMNCGIPYCHNGCPVNNIIPDWNHLVYEDDWQNAL
TVLHSTNNFPEFTGRICPAPCEAACTLNIVDQPVTIKSIEAAIIDRGWREGWVKPEPPENETGKSVAVIGSGPAGLACAQ
QLARAGHAVTVFEKQDRIGGLLRYGIPDFKMEKHLINRRAVQMETEGVTFKTSAEVGVEVSFQALRENFDAIVLSGGAEE
PRPLDIPGAEMSGVRFAMEFLTQQNKRNAGDDEVRAAPRGTLSAKDKHVIVIGGGDTGSDCVGTSNRQGAKSVTQLEIMP
KPPEKEDKALTWPDWPLKLRTSSSHEEGVERDWSVLTKRVVGDGETMTGLECVRIEWRDGQIEEIEGSEFTLPADLILLA
MGFTGPRKRGLLDKAGVTMTERGNVEANEQDYATSEPNVFACGDMRRGQSLVVWAIREGRQCARAVDEALMGVSELPR

Sequences:

>Translated_478_residues
MGKETGFLELDRRERDYIAPEERLKSWREFIKEPPVEELAAQASRCMNCGIPYCHNGCPVNNIIPDWNHLVYEDDWQNAL
TVLHSTNNFPEFTGRICPAPCEAACTLNIVDQPVTIKSIEAAIIDRGWREGWVKPEPPENETGKSVAVIGSGPAGLACAQ
QLARAGHAVTVFEKQDRIGGLLRYGIPDFKMEKHLINRRAVQMETEGVTFKTSAEVGVEVSFQALRENFDAIVLSGGAEE
PRPLDIPGAEMSGVRFAMEFLTQQNKRNAGDDEVRAAPRGTLSAKDKHVIVIGGGDTGSDCVGTSNRQGAKSVTQLEIMP
KPPEKEDKALTWPDWPLKLRTSSSHEEGVERDWSVLTKRVVGDGETMTGLECVRIEWRDGQIEEIEGSEFTLPADLILLA
MGFTGPRKRGLLDKAGVTMTERGNVEANEQDYATSEPNVFACGDMRRGQSLVVWAIREGRQCARAVDEALMGVSELPR
>Mature_477_residues
GKETGFLELDRRERDYIAPEERLKSWREFIKEPPVEELAAQASRCMNCGIPYCHNGCPVNNIIPDWNHLVYEDDWQNALT
VLHSTNNFPEFTGRICPAPCEAACTLNIVDQPVTIKSIEAAIIDRGWREGWVKPEPPENETGKSVAVIGSGPAGLACAQQ
LARAGHAVTVFEKQDRIGGLLRYGIPDFKMEKHLINRRAVQMETEGVTFKTSAEVGVEVSFQALRENFDAIVLSGGAEEP
RPLDIPGAEMSGVRFAMEFLTQQNKRNAGDDEVRAAPRGTLSAKDKHVIVIGGGDTGSDCVGTSNRQGAKSVTQLEIMPK
PPEKEDKALTWPDWPLKLRTSSSHEEGVERDWSVLTKRVVGDGETMTGLECVRIEWRDGQIEEIEGSEFTLPADLILLAM
GFTGPRKRGLLDKAGVTMTERGNVEANEQDYATSEPNVFACGDMRRGQSLVVWAIREGRQCARAVDEALMGVSELPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=467, Percent_Identity=25.6959314775161, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI111118981, Length=128, Percent_Identity=39.84375, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI111118983, Length=128, Percent_Identity=39.84375, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1789606, Length=482, Percent_Identity=38.1742738589212, Blast_Score=268, Evalue=5e-73,
Organism=Escherichia coli, GI1788811, Length=469, Percent_Identity=34.7547974413646, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI87082180, Length=469, Percent_Identity=33.0490405117271, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1788468, Length=435, Percent_Identity=28.2758620689655, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1789243, Length=470, Percent_Identity=26.8085106382979, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17570289, Length=495, Percent_Identity=45.8585858585859, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71984108, Length=464, Percent_Identity=25.6465517241379, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17543860, Length=312, Percent_Identity=26.9230769230769, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=493, Percent_Identity=45.0304259634888, Blast_Score=430, Evalue=1e-121,
Organism=Drosophila melanogaster, GI28574881, Length=488, Percent_Identity=48.9754098360656, Blast_Score=466, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24665539, Length=488, Percent_Identity=48.9754098360656, Blast_Score=466, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24665547, Length=488, Percent_Identity=48.9754098360656, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24665543, Length=488, Percent_Identity=48.9754098360656, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24640763, Length=319, Percent_Identity=26.3322884012539, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI18858217, Length=319, Percent_Identity=26.3322884012539, Blast_Score=95, Evalue=9e-20,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52760; Mature: 52629

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKETGFLELDRRERDYIAPEERLKSWREFIKEPPVEELAAQASRCMNCGIPYCHNGCPV
CCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCC
NNIIPDWNHLVYEDDWQNALTVLHSTNNFPEFTGRICPAPCEAACTLNIVDQPVTIKSIE
CCCCCCCCCEEEECCHHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEEECCCCEEHHHHH
AAIIDRGWREGWVKPEPPENETGKSVAVIGSGPAGLACAQQLARAGHAVTVFEKQDRIGG
HHHHHCHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCHHCH
LLRYGIPDFKMEKHLINRRAVQMETEGVTFKTSAEVGVEVSFQALRENFDAIVLSGGAEE
HHHCCCCCHHHHHHHHHHHHEEEECCCEEEEECCCCCEEEEHHHHHCCCCEEEEECCCCC
PRPLDIPGAEMSGVRFAMEFLTQQNKRNAGDDEVRAAPRGTLSAKDKHVIVIGGGDTGSD
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCCC
CVGTSNRQGAKSVTQLEIMPKPPEKEDKALTWPDWPLKLRTSSSHEEGVERDWSVLTKRV
CCCCCCCCCCCCEEEEEEECCCCCCCCCEECCCCCCEEEECCCCHHHHHHHHHHHHHHHH
VGDGETMTGLECVRIEWRDGQIEEIEGSEFTLPADLILLAMGFTGPRKRGLLDKAGVTMT
HCCCCCCCCEEEEEEEECCCCEEECCCCCEECCHHHHHHHHCCCCCHHCCCHHHCCCEEE
ERGNVEANEQDYATSEPNVFACGDMRRGQSLVVWAIREGRQCARAVDEALMGVSELPR
ECCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GKETGFLELDRRERDYIAPEERLKSWREFIKEPPVEELAAQASRCMNCGIPYCHNGCPV
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCC
NNIIPDWNHLVYEDDWQNALTVLHSTNNFPEFTGRICPAPCEAACTLNIVDQPVTIKSIE
CCCCCCCCCEEEECCHHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEEECCCCEEHHHHH
AAIIDRGWREGWVKPEPPENETGKSVAVIGSGPAGLACAQQLARAGHAVTVFEKQDRIGG
HHHHHCHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCHHCH
LLRYGIPDFKMEKHLINRRAVQMETEGVTFKTSAEVGVEVSFQALRENFDAIVLSGGAEE
HHHCCCCCHHHHHHHHHHHHEEEECCCEEEEECCCCCEEEEHHHHHCCCCEEEEECCCCC
PRPLDIPGAEMSGVRFAMEFLTQQNKRNAGDDEVRAAPRGTLSAKDKHVIVIGGGDTGSD
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCCC
CVGTSNRQGAKSVTQLEIMPKPPEKEDKALTWPDWPLKLRTSSSHEEGVERDWSVLTKRV
CCCCCCCCCCCCEEEEEEECCCCCCCCCEECCCCCCEEEECCCCHHHHHHHHHHHHHHHH
VGDGETMTGLECVRIEWRDGQIEEIEGSEFTLPADLILLAMGFTGPRKRGLLDKAGVTMT
HCCCCCCCCEEEEEEEECCCCEEECCCCCEECCHHHHHHHHCCCCCHHCCCHHHCCCEEE
ERGNVEANEQDYATSEPNVFACGDMRRGQSLVVWAIREGRQCARAVDEALMGVSELPR
ECCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]