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The map label for this gene is acdA [H]

Identifier: 85375184

GI number: 85375184

Start: 2372123

End: 2373289

Strand: Reverse

Name: acdA [H]

Synonym: ELI_11785

Alternate gene names: 85375184

Gene position: 2373289-2372123 (Counterclockwise)

Preceding gene: 85375188

Following gene: 85375183

Centisome position: 77.75

GC content: 64.1

Gene sequence:

>1167_bases
ATGCGTGCCACCCCCGATTTCGATTTCCAGCTCGGCGAAAGCGCCGACATGATCCGCGAAAGCACCGCGCGCTTCGCCGA
TGAACAGATCGCGCTGCTGGCCGAGAAGGTCGACCGCGAGGACTGGTTCCCGCGCGACCTGTGGCGGCAGATGGGCGAAC
TGGGACTCCACGGCATTACCGTGGAAGAAGAGCATGGCGGGCTCGGGCTCGGCTATCTCGAACACGTGATCGCGGTCGAG
GAAATAAGCCGGGCGAGCGCCTCGGTCGGGCTCAGCTATGGCGCCCATTCCAACCTGTGCCTTAACCAGATCCGTCGCTG
GGGCAATGACGAGCAGAAGGCGAAGTACCTGCCCGGCCTGATCTCGGGCGAGCACGTGGGGTCGCTGGCGATGTCGGAAG
CGGCGGCGGGGTCCGACGTGGTCTCGATGAAGCTCAAGGCGGAGGCCGTGCAGGGCGGCTACGTGCTCAATGGCACCAAG
TTCTGGATCACCAATGCGCCGGAAGCCGATACGCTGGTAGTTTACGCGAAAACCGATGGCAGCGCAGGCTCGCGCGGCAT
CACCGCTTTCCTAGTCGAGAAGGACGACGAGGGCTTCTCCATCGGCCAGAAAATCGAAAAGGTCGGTATGCGCGGTTCGC
CAACGGCGGAGCTGGTGTTCGACGATTGCCATATTCCCGAAGACCGCGTGATGGGCCCGGTCAATGGCGGCGTCGGCGTA
CTGATGAGCGGTCTCGATTACGAGCGCGTGGTGCTCGCCGGCTTGCAGCTCGGCATCATGCAGGCGTGTCTCGACACGGT
CATCCCCTACCTGCGCGAGCGCAAGCAGTTCGGCAAGCCGATTGGCTCGTTTCAGCTGATGCAGGCCAAGGTCGCCGACA
TGTATGTCTCGCTGCAATCGGCGCGCGCCTACACCTATGCCGTGGCCAAGAGCTGCGACGCGGAAAAGACCACCCGCTTC
GATGCGGCTGGCGCGATCCTGCTGGCATCGGAGAACGCCTTCCGCGTGGCTGCCGAGAGCGTGCAGGCACTGGGCGGTGC
AGGCTATACGCTCGACTGGCCGGTCGAACGCTACATGCGCGACGCCAAGCTGCTCGATATCGGCGCGGGCACGAACGAGA
TCCGCCGTATGCTTATCGGCCGCGAACTGATCGGGGCGGCGGGATGA

Upstream 100 bases:

>100_bases
GATTTTGCAAATGGACCGCAAGTAACGCCTTGATTTCGCCTGCAACCGGTTTGCGCGGGCCGCGCGGATTGCTATGCGCA
GCGCCAAGGAGATTTGACCG

Downstream 100 bases:

>100_bases
GCGCCGACCGCAAACCATTTTTTCGGTTCAATCCCAACGCTTATGAAATTGGCCGCGCATTTGAGCGAGATGAAGTGCCG
TGCGAAGTGTGCGCTCGCCC

Product: isovaleryl-CoA dehydrogenase

Products: 3-methylbut-2-enoyl-CoA; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MRATPDFDFQLGESADMIRESTARFADEQIALLAEKVDREDWFPRDLWRQMGELGLHGITVEEEHGGLGLGYLEHVIAVE
EISRASASVGLSYGAHSNLCLNQIRRWGNDEQKAKYLPGLISGEHVGSLAMSEAAAGSDVVSMKLKAEAVQGGYVLNGTK
FWITNAPEADTLVVYAKTDGSAGSRGITAFLVEKDDEGFSIGQKIEKVGMRGSPTAELVFDDCHIPEDRVMGPVNGGVGV
LMSGLDYERVVLAGLQLGIMQACLDTVIPYLRERKQFGKPIGSFQLMQAKVADMYVSLQSARAYTYAVAKSCDAEKTTRF
DAAGAILLASENAFRVAAESVQALGGAGYTLDWPVERYMRDAKLLDIGAGTNEIRRMLIGRELIGAAG

Sequences:

>Translated_388_residues
MRATPDFDFQLGESADMIRESTARFADEQIALLAEKVDREDWFPRDLWRQMGELGLHGITVEEEHGGLGLGYLEHVIAVE
EISRASASVGLSYGAHSNLCLNQIRRWGNDEQKAKYLPGLISGEHVGSLAMSEAAAGSDVVSMKLKAEAVQGGYVLNGTK
FWITNAPEADTLVVYAKTDGSAGSRGITAFLVEKDDEGFSIGQKIEKVGMRGSPTAELVFDDCHIPEDRVMGPVNGGVGV
LMSGLDYERVVLAGLQLGIMQACLDTVIPYLRERKQFGKPIGSFQLMQAKVADMYVSLQSARAYTYAVAKSCDAEKTTRF
DAAGAILLASENAFRVAAESVQALGGAGYTLDWPVERYMRDAKLLDIGAGTNEIRRMLIGRELIGAAG
>Mature_388_residues
MRATPDFDFQLGESADMIRESTARFADEQIALLAEKVDREDWFPRDLWRQMGELGLHGITVEEEHGGLGLGYLEHVIAVE
EISRASASVGLSYGAHSNLCLNQIRRWGNDEQKAKYLPGLISGEHVGSLAMSEAAAGSDVVSMKLKAEAVQGGYVLNGTK
FWITNAPEADTLVVYAKTDGSAGSRGITAFLVEKDDEGFSIGQKIEKVGMRGSPTAELVFDDCHIPEDRVMGPVNGGVGV
LMSGLDYERVVLAGLQLGIMQACLDTVIPYLRERKQFGKPIGSFQLMQAKVADMYVSLQSARAYTYAVAKSCDAEKTTRF
DAAGAILLASENAFRVAAESVQALGGAGYTLDWPVERYMRDAKLLDIGAGTNEIRRMLIGRELIGAAG

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI226958412, Length=375, Percent_Identity=58.4, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI226958414, Length=339, Percent_Identity=61.0619469026549, Blast_Score=420, Evalue=1e-118,
Organism=Homo sapiens, GI4557233, Length=375, Percent_Identity=41.0666666666667, Blast_Score=284, Evalue=8e-77,
Organism=Homo sapiens, GI4501859, Length=367, Percent_Identity=38.4196185286104, Blast_Score=263, Evalue=3e-70,
Organism=Homo sapiens, GI21361497, Length=365, Percent_Identity=36.7123287671233, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI7656849, Length=383, Percent_Identity=32.6370757180157, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI4557231, Length=381, Percent_Identity=33.5958005249344, Blast_Score=206, Evalue=4e-53,
Organism=Homo sapiens, GI187960098, Length=381, Percent_Identity=33.5958005249344, Blast_Score=206, Evalue=4e-53,
Organism=Homo sapiens, GI4501857, Length=371, Percent_Identity=31.266846361186, Blast_Score=201, Evalue=8e-52,
Organism=Homo sapiens, GI4557235, Length=354, Percent_Identity=34.7457627118644, Blast_Score=195, Evalue=5e-50,
Organism=Homo sapiens, GI76496475, Length=366, Percent_Identity=33.879781420765, Blast_Score=195, Evalue=5e-50,
Organism=Homo sapiens, GI4503943, Length=395, Percent_Identity=29.3670886075949, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI7669494, Length=370, Percent_Identity=29.7297297297297, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI48976061, Length=291, Percent_Identity=28.5223367697594, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI211971008, Length=291, Percent_Identity=28.5223367697594, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI38505218, Length=284, Percent_Identity=21.830985915493, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1786223, Length=381, Percent_Identity=28.8713910761155, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI87081958, Length=384, Percent_Identity=28.3854166666667, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI87082384, Length=290, Percent_Identity=24.4827586206897, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081702, Length=291, Percent_Identity=25.4295532646048, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17538396, Length=380, Percent_Identity=55.2631578947368, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17508101, Length=372, Percent_Identity=33.6021505376344, Blast_Score=227, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17569725, Length=382, Percent_Identity=34.5549738219895, Blast_Score=221, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI17534899, Length=383, Percent_Identity=33.1592689295039, Blast_Score=221, Evalue=6e-58,
Organism=Caenorhabditis elegans, GI17506239, Length=371, Percent_Identity=32.0754716981132, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17570075, Length=380, Percent_Identity=33.4210526315789, Blast_Score=214, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI17533517, Length=369, Percent_Identity=32.7913279132791, Blast_Score=211, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI71990804, Length=258, Percent_Identity=38.3720930232558, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI71985184, Length=352, Percent_Identity=32.6704545454545, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI71982178, Length=275, Percent_Identity=33.0909090909091, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI32563615, Length=230, Percent_Identity=35.2173913043478, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17551932, Length=368, Percent_Identity=27.7173913043478, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17534353, Length=381, Percent_Identity=26.246719160105, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71985192, Length=285, Percent_Identity=32.280701754386, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17505929, Length=372, Percent_Identity=27.6881720430108, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17531909, Length=228, Percent_Identity=27.1929824561404, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI86563383, Length=317, Percent_Identity=26.813880126183, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI86563381, Length=278, Percent_Identity=27.6978417266187, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562446, Length=285, Percent_Identity=23.1578947368421, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI21355753, Length=379, Percent_Identity=55.1451187335092, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI21356377, Length=374, Percent_Identity=40.9090909090909, Blast_Score=289, Evalue=3e-78,
Organism=Drosophila melanogaster, GI24646207, Length=374, Percent_Identity=38.5026737967914, Blast_Score=261, Evalue=6e-70,
Organism=Drosophila melanogaster, GI24666513, Length=377, Percent_Identity=36.0742705570292, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24660351, Length=382, Percent_Identity=32.4607329842932, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI281363737, Length=366, Percent_Identity=33.6065573770492, Blast_Score=187, Evalue=8e-48,
Organism=Drosophila melanogaster, GI19920834, Length=402, Percent_Identity=27.6119402985075, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.10

Molecular weight: Translated: 41968; Mature: 41968

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00072 ACYL_COA_DH_1 ; PS00073 ACYL_COA_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRATPDFDFQLGESADMIRESTARFADEQIALLAEKVDREDWFPRDLWRQMGELGLHGIT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEE
VEEEHGGLGLGYLEHVIAVEEISRASASVGLSYGAHSNLCLNQIRRWGNDEQKAKYLPGL
EECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHCCCC
ISGEHVGSLAMSEAAAGSDVVSMKLKAEAVQGGYVLNGTKFWITNAPEADTLVVYAKTDG
CCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEECCEEEEEECCCCCCEEEEEEECCC
SAGSRGITAFLVEKDDEGFSIGQKIEKVGMRGSPTAELVFDDCHIPEDRVMGPVNGGVGV
CCCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCHHHCCCCCCCCHHH
LMSGLDYERVVLAGLQLGIMQACLDTVIPYLRERKQFGKPIGSFQLMQAKVADMYVSLQS
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
ARAYTYAVAKSCDAEKTTRFDAAGAILLASENAFRVAAESVQALGGAGYTLDWPVERYMR
HHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEECCCHHHHHC
DAKLLDIGAGTNEIRRMLIGRELIGAAG
CCCEEEECCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRATPDFDFQLGESADMIRESTARFADEQIALLAEKVDREDWFPRDLWRQMGELGLHGIT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEE
VEEEHGGLGLGYLEHVIAVEEISRASASVGLSYGAHSNLCLNQIRRWGNDEQKAKYLPGL
EECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHCCCC
ISGEHVGSLAMSEAAAGSDVVSMKLKAEAVQGGYVLNGTKFWITNAPEADTLVVYAKTDG
CCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEECCEEEEEECCCCCCEEEEEEECCC
SAGSRGITAFLVEKDDEGFSIGQKIEKVGMRGSPTAELVFDDCHIPEDRVMGPVNGGVGV
CCCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCHHHCCCCCCCCHHH
LMSGLDYERVVLAGLQLGIMQACLDTVIPYLRERKQFGKPIGSFQLMQAKVADMYVSLQS
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
ARAYTYAVAKSCDAEKTTRFDAAGAILLASENAFRVAAESVQALGGAGYTLDWPVERYMR
HHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEECCCHHHHHC
DAKLLDIGAGTNEIRRMLIGRELIGAAG
CCCEEEECCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-methylbutanoyl-CoA; acceptor

Specific reaction: 3-methylbutanoyl-CoA + acceptor = 3-methylbut-2-enoyl-CoA + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]