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The map label for this gene is nadB [H]

Identifier: 85375159

GI number: 85375159

Start: 2349242

End: 2350828

Strand: Reverse

Name: nadB [H]

Synonym: ELI_11660

Alternate gene names: 85375159

Gene position: 2350828-2349242 (Counterclockwise)

Preceding gene: 85375161

Following gene: 85375157

Centisome position: 77.02

GC content: 65.41

Gene sequence:

>1587_bases
ATGACTGCGCAATCCCACGACGTCCTGATCATCGGCTCAGGCGCTGCCGGACTGACCGCCGCGCTTGAACTGGCGGAAGA
GAAGAAAGTGCTGGTGCTGGCCAAAGGCTCGCTGACCGGCGGCTCGACAGCCTGGGCGCAGGGCGGGATCGCCGCGGTGC
TCGATGCCGGGGATACGTTCGAGAATCACGTGCGCGACACGATGGTCGCAGGCGCGGGCCTCAACGATCGCGCGACGGTC
GAATTCGTGATCGAGCGCGCGCCCGCGGCCATCGACCGTCTGTGCGAGCTGGGCGTGCCTTTCAATCGCGATGAAGACGC
GCTGCATCTGACGCGCGAAGGCGGGCACAGCCATCGGCGGATCGTCCATGTCGACGACGCGACCGGCTGGGCGGTGCAGG
ATGCGCTGCTGAAGGCGGCCGAGGCCAGTCCCAACATCACCTTGCTGCCGGCGCGCAGCTGTATCGATCTCATCACCGGC
CGACATGGCGAGCGCTTTTCCGGCGACGGCCGGGTATGGGGCGCCTATGCGCTCGACGAAGCGACCGGCAAGGTCGAGGC
GCATGTCGCGCGCGCGACGATCATGGCGGCAGGTGGCGCGGGCCGCGTCTACCAGTTCTCGACCGCGCCGCGCGGGGCGA
CGGGCGACGGGATCGCCATGGCGTGGCGGGCAGGCGCGCGGGTTTCCAACATGGAGATGATGCAGTTCCACCCGACCTGC
CTTTACAATCTCGAGGTCAAGAACTTCCTCATTACCGAGGCCGTGCGCGGCGAGGGCGGGCGGCTCTATCACCCCGAAAC
CGGCCACCGTTTCATGGCGGATTACGATGCCGAACGGATGGAACTGGCCCCGCGCGACGTCGTCGCCCGCGCCATTGACG
ATCAAATCAAGCGCTACGGCCTCGACTATGTCCACCTAGACATCAGCCATGAGCCGCCCGAATTCGTGCGAGAACACTTC
CCTACCATCCACGAGAAACTGTTGGGTCTCGGCATCGACATGACCAAGGGCCCGATCCCCGTGGTCCCGGCGCAGCACTA
TACCTGCGGCGGCGTGCTGGTGGACCTGGATGCGCGCACCGATTTGCCGGGCCTGTGGGCGGCGGGCGAATGCACGGAAA
GCGGGCTGCACGGGGCCAACCGCCTGGCCTCCAACTCGCTGCTCGAATGCTTCGTTTTCGGCGAAGCGGCGGCGGACGAT
ATCCTGTCGCGCTGGGACGAACTGGCCGAACCGCCCGCCATCCGCGAATGGGACGAAAGCCGGGTGACCGATTCCGACGA
GGAGGTCGTCATCAAGCAGAACTGGACCGAGATCCGCCGCTTCATGTGGAACTATGTCGGCATCGTGCGCACCACTAAGC
GCCTCGAACGCGCCCAACATCGCATCAAGCTGCTGCGCAACGAGGTCGACGACTATTACGGCCACTTTCGCGTGACGACC
GACCTGATCGAACTGCGCAACCTGCTGGAGGCAGCAAACCTGATCGTGAAAAGCGCCCTCGCCCGCCACGAAAGCCGCGG
CTTGCACTTCACGCTGGACTATCCGGAAACCGATGAGGTGGCGCGGGATACGATCTTGGTGCCGTAG

Upstream 100 bases:

>100_bases
TGTTACCTTATGCCTAGCGCCGCGCACTGCGCTGTGAAGGCGAGACGCGGCGAAGCGTTCCCCGCTTGCCGCAATGCGTT
TGCCTCGGCATGAGGCGCAC

Downstream 100 bases:

>100_bases
CTTGAAACACTAATCGGTCGCCATCAACCGTTCGTCGGAAACATTGTTACGCCTATAGAAGCGCGATTGCTGCGACTCGC
CGGACAGGTCCATCCCCCTT

Product: aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MTAQSHDVLIIGSGAAGLTAALELAEEKKVLVLAKGSLTGGSTAWAQGGIAAVLDAGDTFENHVRDTMVAGAGLNDRATV
EFVIERAPAAIDRLCELGVPFNRDEDALHLTREGGHSHRRIVHVDDATGWAVQDALLKAAEASPNITLLPARSCIDLITG
RHGERFSGDGRVWGAYALDEATGKVEAHVARATIMAAGGAGRVYQFSTAPRGATGDGIAMAWRAGARVSNMEMMQFHPTC
LYNLEVKNFLITEAVRGEGGRLYHPETGHRFMADYDAERMELAPRDVVARAIDDQIKRYGLDYVHLDISHEPPEFVREHF
PTIHEKLLGLGIDMTKGPIPVVPAQHYTCGGVLVDLDARTDLPGLWAAGECTESGLHGANRLASNSLLECFVFGEAAADD
ILSRWDELAEPPAIREWDESRVTDSDEEVVIKQNWTEIRRFMWNYVGIVRTTKRLERAQHRIKLLRNEVDDYYGHFRVTT
DLIELRNLLEAANLIVKSALARHESRGLHFTLDYPETDEVARDTILVP

Sequences:

>Translated_528_residues
MTAQSHDVLIIGSGAAGLTAALELAEEKKVLVLAKGSLTGGSTAWAQGGIAAVLDAGDTFENHVRDTMVAGAGLNDRATV
EFVIERAPAAIDRLCELGVPFNRDEDALHLTREGGHSHRRIVHVDDATGWAVQDALLKAAEASPNITLLPARSCIDLITG
RHGERFSGDGRVWGAYALDEATGKVEAHVARATIMAAGGAGRVYQFSTAPRGATGDGIAMAWRAGARVSNMEMMQFHPTC
LYNLEVKNFLITEAVRGEGGRLYHPETGHRFMADYDAERMELAPRDVVARAIDDQIKRYGLDYVHLDISHEPPEFVREHF
PTIHEKLLGLGIDMTKGPIPVVPAQHYTCGGVLVDLDARTDLPGLWAAGECTESGLHGANRLASNSLLECFVFGEAAADD
ILSRWDELAEPPAIREWDESRVTDSDEEVVIKQNWTEIRRFMWNYVGIVRTTKRLERAQHRIKLLRNEVDDYYGHFRVTT
DLIELRNLLEAANLIVKSALARHESRGLHFTLDYPETDEVARDTILVP
>Mature_527_residues
TAQSHDVLIIGSGAAGLTAALELAEEKKVLVLAKGSLTGGSTAWAQGGIAAVLDAGDTFENHVRDTMVAGAGLNDRATVE
FVIERAPAAIDRLCELGVPFNRDEDALHLTREGGHSHRRIVHVDDATGWAVQDALLKAAEASPNITLLPARSCIDLITGR
HGERFSGDGRVWGAYALDEATGKVEAHVARATIMAAGGAGRVYQFSTAPRGATGDGIAMAWRAGARVSNMEMMQFHPTCL
YNLEVKNFLITEAVRGEGGRLYHPETGHRFMADYDAERMELAPRDVVARAIDDQIKRYGLDYVHLDISHEPPEFVREHFP
TIHEKLLGLGIDMTKGPIPVVPAQHYTCGGVLVDLDARTDLPGLWAAGECTESGLHGANRLASNSLLECFVFGEAAADDI
LSRWDELAEPPAIREWDESRVTDSDEEVVIKQNWTEIRRFMWNYVGIVRTTKRLERAQHRIKLLRNEVDDYYGHFRVTTD
LIELRNLLEAANLIVKSALARHESRGLHFTLDYPETDEVARDTILVP

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=516, Percent_Identity=32.3643410852713, Blast_Score=206, Evalue=3e-53,
Organism=Escherichia coli, GI1788928, Length=534, Percent_Identity=55.8052434456929, Blast_Score=583, Evalue=1e-167,
Organism=Escherichia coli, GI1790597, Length=522, Percent_Identity=32.9501915708812, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI1786942, Length=566, Percent_Identity=33.2155477031802, Blast_Score=215, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI17505833, Length=536, Percent_Identity=32.089552238806, Blast_Score=214, Evalue=9e-56,
Organism=Caenorhabditis elegans, GI17550100, Length=533, Percent_Identity=32.2701688555347, Blast_Score=212, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6322416, Length=562, Percent_Identity=33.8078291814947, Blast_Score=223, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6322701, Length=559, Percent_Identity=34.1681574239714, Blast_Score=222, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6322511, Length=482, Percent_Identity=25.9336099585062, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320788, Length=320, Percent_Identity=27.5, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI17137288, Length=532, Percent_Identity=32.7067669172932, Blast_Score=215, Evalue=6e-56,
Organism=Drosophila melanogaster, GI24655642, Length=532, Percent_Identity=32.7067669172932, Blast_Score=215, Evalue=6e-56,
Organism=Drosophila melanogaster, GI24655647, Length=532, Percent_Identity=32.7067669172932, Blast_Score=215, Evalue=6e-56,
Organism=Drosophila melanogaster, GI24663005, Length=549, Percent_Identity=30.0546448087432, Blast_Score=188, Evalue=6e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 58077; Mature: 57946

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQSHDVLIIGSGAAGLTAALELAEEKKVLVLAKGSLTGGSTAWAQGGIAAVLDAGDTF
CCCCCCCEEEEECCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCEEEEEECCCHH
ENHVRDTMVAGAGLNDRATVEFVIERAPAAIDRLCELGVPFNRDEDALHLTREGGHSHRR
HHHHHHHHHCCCCCCCCCCEEHHHHHCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCE
IVHVDDATGWAVQDALLKAAEASPNITLLPARSCIDLITGRHGERFSGDGRVWGAYALDE
EEEECCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCEEEEEEECC
ATGKVEAHVARATIMAAGGAGRVYQFSTAPRGATGDGIAMAWRAGARVSNMEMMQFHPTC
CCCCHHHHEEEEEEEECCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCCEEECCEE
LYNLEVKNFLITEAVRGEGGRLYHPETGHRFMADYDAERMELAPRDVVARAIDDQIKRYG
EEEEEHHHEEEEHHHCCCCCEEECCCCCCEEEECCCCHHHHCCHHHHHHHHHHHHHHHCC
LDYVHLDISHEPPEFVREHFPTIHEKLLGLGIDMTKGPIPVVPAQHYTCGGVLVDLDART
CCEEEEECCCCCHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCEECCCEEEEECCCC
DLPGLWAAGECTESGLHGANRLASNSLLECFVFGEAAADDILSRWDELAEPPAIREWDES
CCCCCCCCCCHHHHCCCHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCC
RVTDSDEEVVIKQNWTEIRRFMWNYVGIVRTTKRLERAQHRIKLLRNEVDDYYGHFRVTT
CCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHH
DLIELRNLLEAANLIVKSALARHESRGLHFTLDYPETDEVARDTILVP
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCC
>Mature Secondary Structure 
TAQSHDVLIIGSGAAGLTAALELAEEKKVLVLAKGSLTGGSTAWAQGGIAAVLDAGDTF
CCCCCCEEEEECCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCEEEEEECCCHH
ENHVRDTMVAGAGLNDRATVEFVIERAPAAIDRLCELGVPFNRDEDALHLTREGGHSHRR
HHHHHHHHHCCCCCCCCCCEEHHHHHCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCE
IVHVDDATGWAVQDALLKAAEASPNITLLPARSCIDLITGRHGERFSGDGRVWGAYALDE
EEEECCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCEEEEEEECC
ATGKVEAHVARATIMAAGGAGRVYQFSTAPRGATGDGIAMAWRAGARVSNMEMMQFHPTC
CCCCHHHHEEEEEEEECCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCCEEECCEE
LYNLEVKNFLITEAVRGEGGRLYHPETGHRFMADYDAERMELAPRDVVARAIDDQIKRYG
EEEEEHHHEEEEHHHCCCCCEEECCCCCCEEEECCCCHHHHCCHHHHHHHHHHHHHHHCC
LDYVHLDISHEPPEFVREHFPTIHEKLLGLGIDMTKGPIPVVPAQHYTCGGVLVDLDART
CCEEEEECCCCCHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCEECCCEEEEECCCC
DLPGLWAAGECTESGLHGANRLASNSLLECFVFGEAAADDILSRWDELAEPPAIREWDES
CCCCCCCCCCHHHHCCCHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCC
RVTDSDEEVVIKQNWTEIRRFMWNYVGIVRTTKRLERAQHRIKLLRNEVDDYYGHFRVTT
CCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHH
DLIELRNLLEAANLIVKSALARHESRGLHFTLDYPETDEVARDTILVP
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7737518; 10984043; 7961421 [H]