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The map label for this gene is nrdA [H]

Identifier: 85375154

GI number: 85375154

Start: 2344377

End: 2346422

Strand: Reverse

Name: nrdA [H]

Synonym: ELI_11635

Alternate gene names: 85375154

Gene position: 2346422-2344377 (Counterclockwise)

Preceding gene: 85375155

Following gene: 85375153

Centisome position: 76.87

GC content: 62.12

Gene sequence:

>2046_bases
ATGGACTTCAAGGCAGGGGACGATACGGCCATGGGCGCAGACGCGCTGGACACTTCCGAGATGACGGACGCTCCGGAAAA
GGCCGTCGGGAAAGACGCCAAGAGCAAGGAAAAGGCGGTCGCTATGGACAAGCAGGATGCAGGCTCGGCAGAACTGGTCG
CGGATGCCGCTGCCGGTGCGCTGGCGGAAGCGGCGAAGGCCGCCGCCAAGCCTGTCGACGACAGCAAGAAAGTGAACGAT
CGCCGTTTTACCATCGAAATCGACGAATCGCGCGATGTGAACCTGACCGAATTCGGCAAGGAAACGCTGATCGATCGCTA
CCTGCTGCCGGGCGAAAAGTTCCAGGACCTGTTCGCGCGCGTCGCGGATGCCTATGCCGACGACCAGGAGCATGCGCAGC
GGCTCTACGACTACATCTCGCGCCTGTGGTTCATGCCGGCGACCCCGGTGCTGTCCAATGGCGGCACCAATCGCGGCCTG
CCGATTTCCTGCTACCTCAACTCGGTCTCCGACAGCCTCGACGGGATCGTCGGCACTTGGAACGAGAATGTCTGGCTCGC
TTCCAAGGGCGGCGGCATCGGCACCTATTGGGGCAATGTCCGCGGCATCGGCGAGCCGGTCGGCCTCAACGGCAAGACCA
GCGGCATCATCCCCTTCGTGCGCGTGATGGATTCGCTCACGCTGGCGATTTCGCAGGGTTCGCTGCGGCGCGGCTCGGCG
GCCTGCTATATCGACATCCACCACCCGGAGATCGAGGAATTCCTCGAAATCCGCAAACCCTCGGGTGACTTCAACCGCAA
GGCGCTGAACCTGCACCACGGCGTGCTGATCACCGACGCATTCATGGAAGCCGTGCGCGAAGGCGCCGAGTTCGATCTCA
AATCGCCCAAGGACGGCAGCGTGCGCGGCACCGTCGATGCGCGCAGCCTGTTCCAGAAGCTGGTCGAGACCCGCCTGGCG
ACCGGCGAGCCCTATATCGTCTTCGCCGACACCGTGAACCGCATGATGCCCAAGCATCACCGCGATCTCGGCCTCAAGGT
CTCGACCTCCAACCTGTGTTCGGAAATCACTCTGCCGACCGGGGTCGACCATCTCGGCAACGACCGCACGGCGGTGTGCT
GCCTGTCCTCGCTCAACCTCGAGAAGTGGGACGAGTGGAACGGCGAGAAGGACTTCATCGAGGATGTCATGCGCTTCCTC
GACAATGTCCTGCAGGACTATATCGACCGCGCGCCCGACGAGATGGCGCGGGCGAAATATTCCGCCAGCCGCGAACGCAG
CGTCGGCATGGGGGTGATGGGCTTCCACTCCTTCCTGCAGTCGAAGAACCTGCCGCTCGAAGGGCCGATGGCGAAGGCCT
GGAACGACAAGATGTTCAAGCAGATCCATGCCAAGGCCAACGAGGCCTCGATGCTGCTCGCCAAGGAACGCGGGCCGTGC
CCCGATGCAGAGGAAATGGGCGCGATGGAGCGCTTCAGCTGCAAGATGGCGATCGCGCCGACCGCGTCGATCAGCATTAT
CTGCGGCGGCACCAGCGCCTGCATCGAGCCGATCCCGGCCAATATCTACACCCACAAGACGCTGTCGGGCTCCTTCATCG
TCAAGAACCCGTATCTCGAGAAATTGCTCAAGAAGAAGAGCAAGGATTCGACGGCGGTGTGGAACTCGATCCTCGAGCGC
GGCGGCTCGGTCGCGCATCTTGATTTCCTGACGCCGGAAGAAAAGGCGGTCTTCAAGACCAGCTTCGAGATCGACCAGCG
CTACCTGCTCGAATTCGCCGCCGACCGCGCGCCCTATATCGACCAGGCCCAGTCGCTGAACCTGTTCATCCCGGCCGATG
TCGACAAGTGGGATTTGATGATGCTGCACTTCCAGGCGTGGGAGAAAGGCATCAAGTCGCTCTATTACCTCCGTTCCAAG
AGCGTGCAGCGCGCGGGCTTTGCCGGCGGTGTCGAGGCGGACAACACTAAGGATGCAGCGCAATTCGAACTGAGCGCGCA
GGCGGGTGAAGAGACCGACTACGAAGAATGCCTGAGCTGCCAGTAG

Upstream 100 bases:

>100_bases
GTGCAGGTGCGCCCATCAATCCTCTCGACGCAGCGGAACGAATGTGGAACAAAGGGCGGCTCGCGAGTCGCCTTTCCGAT
TGAGAGGACGGGGCGAGAGA

Downstream 100 bases:

>100_bases
GCTGCCGCTTTTGACCCTCACCCTCCCAGCGCTGCGCGCCGGGCCCCTCCCTCTCCCGGTGGGAGAGGGAAAGAGCGCCG
CAGGCGTGAAAGGGTGAGGG

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase [H]

Number of amino acids: Translated: 681; Mature: 681

Protein sequence:

>681_residues
MDFKAGDDTAMGADALDTSEMTDAPEKAVGKDAKSKEKAVAMDKQDAGSAELVADAAAGALAEAAKAAAKPVDDSKKVND
RRFTIEIDESRDVNLTEFGKETLIDRYLLPGEKFQDLFARVADAYADDQEHAQRLYDYISRLWFMPATPVLSNGGTNRGL
PISCYLNSVSDSLDGIVGTWNENVWLASKGGGIGTYWGNVRGIGEPVGLNGKTSGIIPFVRVMDSLTLAISQGSLRRGSA
ACYIDIHHPEIEEFLEIRKPSGDFNRKALNLHHGVLITDAFMEAVREGAEFDLKSPKDGSVRGTVDARSLFQKLVETRLA
TGEPYIVFADTVNRMMPKHHRDLGLKVSTSNLCSEITLPTGVDHLGNDRTAVCCLSSLNLEKWDEWNGEKDFIEDVMRFL
DNVLQDYIDRAPDEMARAKYSASRERSVGMGVMGFHSFLQSKNLPLEGPMAKAWNDKMFKQIHAKANEASMLLAKERGPC
PDAEEMGAMERFSCKMAIAPTASISIICGGTSACIEPIPANIYTHKTLSGSFIVKNPYLEKLLKKKSKDSTAVWNSILER
GGSVAHLDFLTPEEKAVFKTSFEIDQRYLLEFAADRAPYIDQAQSLNLFIPADVDKWDLMMLHFQAWEKGIKSLYYLRSK
SVQRAGFAGGVEADNTKDAAQFELSAQAGEETDYEECLSCQ

Sequences:

>Translated_681_residues
MDFKAGDDTAMGADALDTSEMTDAPEKAVGKDAKSKEKAVAMDKQDAGSAELVADAAAGALAEAAKAAAKPVDDSKKVND
RRFTIEIDESRDVNLTEFGKETLIDRYLLPGEKFQDLFARVADAYADDQEHAQRLYDYISRLWFMPATPVLSNGGTNRGL
PISCYLNSVSDSLDGIVGTWNENVWLASKGGGIGTYWGNVRGIGEPVGLNGKTSGIIPFVRVMDSLTLAISQGSLRRGSA
ACYIDIHHPEIEEFLEIRKPSGDFNRKALNLHHGVLITDAFMEAVREGAEFDLKSPKDGSVRGTVDARSLFQKLVETRLA
TGEPYIVFADTVNRMMPKHHRDLGLKVSTSNLCSEITLPTGVDHLGNDRTAVCCLSSLNLEKWDEWNGEKDFIEDVMRFL
DNVLQDYIDRAPDEMARAKYSASRERSVGMGVMGFHSFLQSKNLPLEGPMAKAWNDKMFKQIHAKANEASMLLAKERGPC
PDAEEMGAMERFSCKMAIAPTASISIICGGTSACIEPIPANIYTHKTLSGSFIVKNPYLEKLLKKKSKDSTAVWNSILER
GGSVAHLDFLTPEEKAVFKTSFEIDQRYLLEFAADRAPYIDQAQSLNLFIPADVDKWDLMMLHFQAWEKGIKSLYYLRSK
SVQRAGFAGGVEADNTKDAAQFELSAQAGEETDYEECLSCQ
>Mature_681_residues
MDFKAGDDTAMGADALDTSEMTDAPEKAVGKDAKSKEKAVAMDKQDAGSAELVADAAAGALAEAAKAAAKPVDDSKKVND
RRFTIEIDESRDVNLTEFGKETLIDRYLLPGEKFQDLFARVADAYADDQEHAQRLYDYISRLWFMPATPVLSNGGTNRGL
PISCYLNSVSDSLDGIVGTWNENVWLASKGGGIGTYWGNVRGIGEPVGLNGKTSGIIPFVRVMDSLTLAISQGSLRRGSA
ACYIDIHHPEIEEFLEIRKPSGDFNRKALNLHHGVLITDAFMEAVREGAEFDLKSPKDGSVRGTVDARSLFQKLVETRLA
TGEPYIVFADTVNRMMPKHHRDLGLKVSTSNLCSEITLPTGVDHLGNDRTAVCCLSSLNLEKWDEWNGEKDFIEDVMRFL
DNVLQDYIDRAPDEMARAKYSASRERSVGMGVMGFHSFLQSKNLPLEGPMAKAWNDKMFKQIHAKANEASMLLAKERGPC
PDAEEMGAMERFSCKMAIAPTASISIICGGTSACIEPIPANIYTHKTLSGSFIVKNPYLEKLLKKKSKDSTAVWNSILER
GGSVAHLDFLTPEEKAVFKTSFEIDQRYLLEFAADRAPYIDQAQSLNLFIPADVDKWDLMMLHFQAWEKGIKSLYYLRSK
SVQRAGFAGGVEADNTKDAAQFELSAQAGEETDYEECLSCQ

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 ATP-cone domains [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=637, Percent_Identity=31.2401883830455, Blast_Score=271, Evalue=1e-72,
Organism=Escherichia coli, GI1789030, Length=626, Percent_Identity=26.3578274760383, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI2367133, Length=439, Percent_Identity=28.4738041002278, Blast_Score=132, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17555242, Length=655, Percent_Identity=31.1450381679389, Blast_Score=272, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6320914, Length=671, Percent_Identity=30.849478390462, Blast_Score=263, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6322123, Length=627, Percent_Identity=30.7814992025518, Blast_Score=258, Evalue=3e-69,
Organism=Drosophila melanogaster, GI17352481, Length=633, Percent_Identity=29.6998420221169, Blast_Score=251, Evalue=8e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005144
- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926 [H]

Pfam domain/function: PF03477 ATP-cone; PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 75029; Mature: 75029

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKAGDDTAMGADALDTSEMTDAPEKAVGKDAKSKEKAVAMDKQDAGSAELVADAAAGA
CCCCCCCCCCCCCCCCCCHHHCCCCHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHH
LAEAAKAAAKPVDDSKKVNDRRFTIEIDESRDVNLTEFGKETLIDRYLLPGEKFQDLFAR
HHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
VADAYADDQEHAQRLYDYISRLWFMPATPVLSNGGTNRGLPISCYLNSVSDSLDGIVGTW
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCEEEEEECHHHHHHCCEEEEC
NENVWLASKGGGIGTYWGNVRGIGEPVGLNGKTSGIIPFVRVMDSLTLAISQGSLRRGSA
CCCEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEEECCCCCCCCCE
ACYIDIHHPEIEEFLEIRKPSGDFNRKALNLHHGVLITDAFMEAVREGAEFDLKSPKDGS
EEEEEECCCCHHHHHHHCCCCCCCCCCEEEECCCEEHHHHHHHHHHCCCCCCCCCCCCCC
VRGTVDARSLFQKLVETRLATGEPYIVFADTVNRMMPKHHRDLGLKVSTSNLCSEITLPT
EEECHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHCCHHHHCCCCEEECHHHHHHCCCCC
GVDHLGNDRTAVCCLSSLNLEKWDEWNGEKDFIEDVMRFLDNVLQDYIDRAPDEMARAKY
CCHHCCCCCEEEEEEHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
SASRERSVGMGVMGFHSFLQSKNLPLEGPMAKAWNDKMFKQIHAKANEASMLLAKERGPC
HHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHEEEECCCCCC
PDAEEMGAMERFSCKMAIAPTASISIICGGTSACIEPIPANIYTHKTLSGSFIVKNPYLE
CCHHHHCCHHHHCEEEEECCCCEEEEEECCCHHHHCCCCCCEEEEEECCCCEEEECHHHH
KLLKKKSKDSTAVWNSILERGGSVAHLDFLTPEEKAVFKTSFEIDQRYLLEFAADRAPYI
HHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHEEHHHHHHHHHHHHHHHCCCCCC
DQAQSLNLFIPADVDKWDLMMLHFQAWEKGIKSLYYLRSKSVQRAGFAGGVEADNTKDAA
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
QFELSAQAGEETDYEECLSCQ
HEEEECCCCCCCCHHHHHCCC
>Mature Secondary Structure
MDFKAGDDTAMGADALDTSEMTDAPEKAVGKDAKSKEKAVAMDKQDAGSAELVADAAAGA
CCCCCCCCCCCCCCCCCCHHHCCCCHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHH
LAEAAKAAAKPVDDSKKVNDRRFTIEIDESRDVNLTEFGKETLIDRYLLPGEKFQDLFAR
HHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
VADAYADDQEHAQRLYDYISRLWFMPATPVLSNGGTNRGLPISCYLNSVSDSLDGIVGTW
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCEEEEEECHHHHHHCCEEEEC
NENVWLASKGGGIGTYWGNVRGIGEPVGLNGKTSGIIPFVRVMDSLTLAISQGSLRRGSA
CCCEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEEECCCCCCCCCE
ACYIDIHHPEIEEFLEIRKPSGDFNRKALNLHHGVLITDAFMEAVREGAEFDLKSPKDGS
EEEEEECCCCHHHHHHHCCCCCCCCCCEEEECCCEEHHHHHHHHHHCCCCCCCCCCCCCC
VRGTVDARSLFQKLVETRLATGEPYIVFADTVNRMMPKHHRDLGLKVSTSNLCSEITLPT
EEECHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHCCHHHHCCCCEEECHHHHHHCCCCC
GVDHLGNDRTAVCCLSSLNLEKWDEWNGEKDFIEDVMRFLDNVLQDYIDRAPDEMARAKY
CCHHCCCCCEEEEEEHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
SASRERSVGMGVMGFHSFLQSKNLPLEGPMAKAWNDKMFKQIHAKANEASMLLAKERGPC
HHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHEEEECCCCCC
PDAEEMGAMERFSCKMAIAPTASISIICGGTSACIEPIPANIYTHKTLSGSFIVKNPYLE
CCHHHHCCHHHHCEEEEECCCCEEEEEECCCHHHHCCCCCCEEEEEECCCCEEEECHHHH
KLLKKKSKDSTAVWNSILERGGSVAHLDFLTPEEKAVFKTSFEIDQRYLLEFAADRAPYI
HHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHEEHHHHHHHHHHHHHHHCCCCCC
DQAQSLNLFIPADVDKWDLMMLHFQAWEKGIKSLYYLRSKSVQRAGFAGGVEADNTKDAA
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
QFELSAQAGEETDYEECLSCQ
HEEEECCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]