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The map label for this gene is nrdF

Identifier: 85375147

GI number: 85375147

Start: 2339890

End: 2340960

Strand: Reverse

Name: nrdF

Synonym: ELI_11600

Alternate gene names: 85375147

Gene position: 2340960-2339890 (Counterclockwise)

Preceding gene: 85375150

Following gene: 85375146

Centisome position: 76.69

GC content: 60.69

Gene sequence:

>1071_bases
ATGTCCCTTCTTGAAGCCCGTAAGACCTACAAGCCCTTCGAATATCCGTGGGCCTACGACTTCTGGAAACGCCAGCAGCA
GATCCACTGGATGCCCGAAGAAGTGCCGCTGGGCGAAGACTGCCGCGACTGGGCGCAGAAGATCACCGAGCACGAGCGCA
ACCTGCTCACGCAGATCTTCCGTTTCTTCACCCAGGCCGATGTCGAAGTGCAGGATTGCTACCACGACAAATACGGCCGC
GTGTTCAAGCCGACCGAGATCAAGATGATGCTGACCGCTTTCTCCAACATGGAGACGGTGCACATCGCGGCCTACAGCCA
CCTGCTCGACACGATCGGCATGCCCGAAAGCGAATATTCCGCCTTCCTCGAATACGAGGAGATGAAGGACAAGCACGATT
ACCTCCAGCTGTTCGGCGTGGACACGGACGAGGATATCGCGCGCACGCTGGCGGTGTTCGGCGGCTTCACCGAAGGCATG
CAGCTGTTCGCCAGCTTCGCCATGCTGATGAACTTCCCGCGCTTCAACAAGATGAAGGGCATGGGCCAGATCGTCAGCTG
GTCGGTCCGCGACGAGAGCCTGCACTGCGAAGGCATCGTGCGGCTGTTCCACGAATTCGTGCGCGAGCGCGATTGCTACA
CCAAGGCGGTGAAGGAAGACATCATCGACATCTGCCAGAAGTCGGTGCGGCTGGAAGACAACTTCATCGACCTCGCCTTC
GAAATGGGTCCGGTTTCCGGGATGACGCCGAAGGAAATCAAGAAATACATCCGCTACATCGCCGACTGGCGGCTCGGCCA
GCTCGGCCTGCAGCCGATCTACATGGTCGACGACCACCCGCTCCCCTGGCTCGCCCCGCTCCTGAACGGCGTCGAACACG
CCAACTTCTTCGAACAGCGCGCCACCGAATATTCCAAGGGCGCGACCAAGGGCAACTGGCAGGATGTGTGGAGCACCTTC
GACAAGCGCCACGGCGCCGGCAAGGCCGCGAACGAGGAAGAGGCGGTGGACGACGGGCCGGATATGTTTGGGGACGAAGG
TGGCAGCGCCGGGAGTGTGGCGGCGGAGTGA

Upstream 100 bases:

>100_bases
CCCAACCGGAGAACCACAAGATGTCGTTGCTAGAAGCCCGTAAGACTGACTAGCTCTCCACCACATATTGATTTGCTGCA
CACCAGCCGAGGAAACGTCC

Downstream 100 bases:

>100_bases
TGTTAGTTGCTTAGAACTAGTCCTTGTCTATACTTGGATGATGGCTACCAAATATCTTCCGATCAATTATCGTCATTCTG
CAGTCTCCGGCGAAGGTTCC

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSLLEARKTYKPFEYPWAYDFWKRQQQIHWMPEEVPLGEDCRDWAQKITEHERNLLTQIFRFFTQADVEVQDCYHDKYGR
VFKPTEIKMMLTAFSNMETVHIAAYSHLLDTIGMPESEYSAFLEYEEMKDKHDYLQLFGVDTDEDIARTLAVFGGFTEGM
QLFASFAMLMNFPRFNKMKGMGQIVSWSVRDESLHCEGIVRLFHEFVRERDCYTKAVKEDIIDICQKSVRLEDNFIDLAF
EMGPVSGMTPKEIKKYIRYIADWRLGQLGLQPIYMVDDHPLPWLAPLLNGVEHANFFEQRATEYSKGATKGNWQDVWSTF
DKRHGAGKAANEEEAVDDGPDMFGDEGGSAGSVAAE

Sequences:

>Translated_356_residues
MSLLEARKTYKPFEYPWAYDFWKRQQQIHWMPEEVPLGEDCRDWAQKITEHERNLLTQIFRFFTQADVEVQDCYHDKYGR
VFKPTEIKMMLTAFSNMETVHIAAYSHLLDTIGMPESEYSAFLEYEEMKDKHDYLQLFGVDTDEDIARTLAVFGGFTEGM
QLFASFAMLMNFPRFNKMKGMGQIVSWSVRDESLHCEGIVRLFHEFVRERDCYTKAVKEDIIDICQKSVRLEDNFIDLAF
EMGPVSGMTPKEIKKYIRYIADWRLGQLGLQPIYMVDDHPLPWLAPLLNGVEHANFFEQRATEYSKGATKGNWQDVWSTF
DKRHGAGKAANEEEAVDDGPDMFGDEGGSAGSVAAE
>Mature_355_residues
SLLEARKTYKPFEYPWAYDFWKRQQQIHWMPEEVPLGEDCRDWAQKITEHERNLLTQIFRFFTQADVEVQDCYHDKYGRV
FKPTEIKMMLTAFSNMETVHIAAYSHLLDTIGMPESEYSAFLEYEEMKDKHDYLQLFGVDTDEDIARTLAVFGGFTEGMQ
LFASFAMLMNFPRFNKMKGMGQIVSWSVRDESLHCEGIVRLFHEFVRERDCYTKAVKEDIIDICQKSVRLEDNFIDLAFE
MGPVSGMTPKEIKKYIRYIADWRLGQLGLQPIYMVDDHPLPWLAPLLNGVEHANFFEQRATEYSKGATKGNWQDVWSTFD
KRHGAGKAANEEEAVDDGPDMFGDEGGSAGSVAAE

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=315, Percent_Identity=26.984126984127, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI289177074, Length=315, Percent_Identity=26.984126984127, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI260064013, Length=300, Percent_Identity=26.6666666666667, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI4557845, Length=300, Percent_Identity=26.6666666666667, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI289177076, Length=294, Percent_Identity=26.530612244898, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1788567, Length=296, Percent_Identity=20.6081081081081, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789031, Length=296, Percent_Identity=22.972972972973, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17551968, Length=314, Percent_Identity=25.796178343949, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322434, Length=310, Percent_Identity=26.1290322580645, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI18079279, Length=315, Percent_Identity=26.984126984127, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 41288; Mature: 41157

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLEARKTYKPFEYPWAYDFWKRQQQIHWMPEEVPLGEDCRDWAQKITEHERNLLTQIF
CCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RFFTQADVEVQDCYHDKYGRVFKPTEIKMMLTAFSNMETVHIAAYSHLLDTIGMPESEYS
HHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCHHHHH
AFLEYEEMKDKHDYLQLFGVDTDEDIARTLAVFGGFTEGMQLFASFAMLMNFPRFNKMKG
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHCC
MGQIVSWSVRDESLHCEGIVRLFHEFVRERDCYTKAVKEDIIDICQKSVRLEDNFIDLAF
CCHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHE
EMGPVSGMTPKEIKKYIRYIADWRLGQLGLQPIYMVDDHPLPWLAPLLNGVEHANFFEQR
ECCCCCCCCHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
ATEYSKGATKGNWQDVWSTFDKRHGAGKAANEEEAVDDGPDMFGDEGGSAGSVAAE
HHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLLEARKTYKPFEYPWAYDFWKRQQQIHWMPEEVPLGEDCRDWAQKITEHERNLLTQIF
CHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RFFTQADVEVQDCYHDKYGRVFKPTEIKMMLTAFSNMETVHIAAYSHLLDTIGMPESEYS
HHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCHHHHH
AFLEYEEMKDKHDYLQLFGVDTDEDIARTLAVFGGFTEGMQLFASFAMLMNFPRFNKMKG
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHCC
MGQIVSWSVRDESLHCEGIVRLFHEFVRERDCYTKAVKEDIIDICQKSVRLEDNFIDLAF
CCHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHE
EMGPVSGMTPKEIKKYIRYIADWRLGQLGLQPIYMVDDHPLPWLAPLLNGVEHANFFEQR
ECCCCCCCCHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
ATEYSKGATKGNWQDVWSTFDKRHGAGKAANEEEAVDDGPDMFGDEGGSAGSVAAE
HHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]