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The map label for this gene is folD

Identifier: 85375133

GI number: 85375133

Start: 2330753

End: 2331649

Strand: Reverse

Name: folD

Synonym: ELI_11530

Alternate gene names: 85375133

Gene position: 2331649-2330753 (Counterclockwise)

Preceding gene: 85375134

Following gene: 85375132

Centisome position: 76.39

GC content: 64.55

Gene sequence:

>897_bases
ATGACCGCAGAGATCATCGACGGAAAAGCCTTCGCTGCACGCCTGCGTGAACGCGTCGGGGCGCTGGCCGAGAAATTCGA
AGCCGCAGCCGGCCGCAAGCCCGGCCTCGCCGTCGTGCTCGTCGGCGAAGACCCGGCAAGCGAAGTCTATGTCCGCAACA
AGGGCAAGGCGACGCTGGCGGCCAATATGGAGAGCTTCGAGTACAAGCTGCCTGCGGATACGACTGCGCAAGACCTCCTG
GCTCTCGTGAAGAAACTGAACGGTGACCCGGCGGTGGACGGGATCCTCGTCCAGCTCCCTTTGCCCGACCATCTTGACGA
ACAATCGATCATCGCCGCGATCAGTCCGGACAAGGATGTCGACGGTTTCCATGTCATCAATGCCGGACGGCTGTCGGTCG
GCCAGCGTGGGTTCGTGCCCTGCACGCCGCTCGGCTGCATGATGCTGTTGGCTGACCGGCTAGGAGACCTTTCGGGTCTC
GAAGCGGTCGTGATCGGGCGCTCCAATATCGTCGGCAAGCCGATGGCGCAGCTTCTGCTCGACGCCAACGCCACCGTAAC
CATCGCGCACAGCCGGACCAGGAACCTGCCCGAGGTCGTCAAGCGCGCCGATATCGTCGTGGCTGCCGTGGGCCGCGCTG
AGATGGTCAAACCCGAATGGCTGAAGGACGGCGCGACGGTGATCGATGTCGGCATCAACCGGCTTCCGCCCGAGCCGGGC
AAGGAGCGTGGGAGGCTCGTCGGCGATGTCGACTTCGGCAGGGCGAGCGAGGTCGCAGCTGCAATCACGCCAGTGCCCGG
CGGTGTCGGCCCGATGACGATCGCCGTCCTGCTCAGGAACACGCTGGTTGCGGCCCATCGCAATGAGGGGATCGACCTGC
CGGAAGATGCGATCTGA

Upstream 100 bases:

>100_bases
TCATGCCGAGCACGGGGGCGATCGATTTCTCCCCGCTTGTCCTCATCATCGTGCTGCAAGTGATGTTGATCGTCCTGACG
AATGTCGCCATTGCGGCGCA

Downstream 100 bases:

>100_bases
TGCGCGCGCTCGTTCTCGGCTTGGCCTGCGCTCTGGCTGCGTGTGCTTCGGGCCCGCAGCGGGGCGATTTCCCGCGCATT
CCGCTGACAGCGAACCCGAG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTAEIIDGKAFAARLRERVGALAEKFEAAAGRKPGLAVVLVGEDPASEVYVRNKGKATLAANMESFEYKLPADTTAQDLL
ALVKKLNGDPAVDGILVQLPLPDHLDEQSIIAAISPDKDVDGFHVINAGRLSVGQRGFVPCTPLGCMMLLADRLGDLSGL
EAVVIGRSNIVGKPMAQLLLDANATVTIAHSRTRNLPEVVKRADIVVAAVGRAEMVKPEWLKDGATVIDVGINRLPPEPG
KERGRLVGDVDFGRASEVAAAITPVPGGVGPMTIAVLLRNTLVAAHRNEGIDLPEDAI

Sequences:

>Translated_298_residues
MTAEIIDGKAFAARLRERVGALAEKFEAAAGRKPGLAVVLVGEDPASEVYVRNKGKATLAANMESFEYKLPADTTAQDLL
ALVKKLNGDPAVDGILVQLPLPDHLDEQSIIAAISPDKDVDGFHVINAGRLSVGQRGFVPCTPLGCMMLLADRLGDLSGL
EAVVIGRSNIVGKPMAQLLLDANATVTIAHSRTRNLPEVVKRADIVVAAVGRAEMVKPEWLKDGATVIDVGINRLPPEPG
KERGRLVGDVDFGRASEVAAAITPVPGGVGPMTIAVLLRNTLVAAHRNEGIDLPEDAI
>Mature_297_residues
TAEIIDGKAFAARLRERVGALAEKFEAAAGRKPGLAVVLVGEDPASEVYVRNKGKATLAANMESFEYKLPADTTAQDLLA
LVKKLNGDPAVDGILVQLPLPDHLDEQSIIAAISPDKDVDGFHVINAGRLSVGQRGFVPCTPLGCMMLLADRLGDLSGLE
AVVIGRSNIVGKPMAQLLLDANATVTIAHSRTRNLPEVVKRADIVVAAVGRAEMVKPEWLKDGATVIDVGINRLPPEPGK
ERGRLVGDVDFGRASEVAAAITPVPGGVGPMTIAVLLRNTLVAAHRNEGIDLPEDAI

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=44.8275862068966, Blast_Score=234, Evalue=7e-62,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=43.3898305084746, Blast_Score=221, Evalue=5e-58,
Organism=Homo sapiens, GI94721354, Length=295, Percent_Identity=44.4067796610169, Blast_Score=221, Evalue=7e-58,
Organism=Homo sapiens, GI36796743, Length=228, Percent_Identity=26.7543859649123, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1786741, Length=285, Percent_Identity=50.1754385964912, Blast_Score=257, Evalue=6e-70,
Organism=Caenorhabditis elegans, GI17568735, Length=295, Percent_Identity=45.4237288135593, Blast_Score=220, Evalue=9e-58,
Organism=Saccharomyces cerevisiae, GI6319558, Length=288, Percent_Identity=48.9583333333333, Blast_Score=268, Evalue=9e-73,
Organism=Saccharomyces cerevisiae, GI6321643, Length=300, Percent_Identity=44.6666666666667, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6322933, Length=310, Percent_Identity=25.4838709677419, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI62472483, Length=292, Percent_Identity=47.6027397260274, Blast_Score=248, Evalue=4e-66,
Organism=Drosophila melanogaster, GI45551871, Length=292, Percent_Identity=47.6027397260274, Blast_Score=248, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24645718, Length=292, Percent_Identity=47.6027397260274, Blast_Score=248, Evalue=5e-66,
Organism=Drosophila melanogaster, GI17137370, Length=292, Percent_Identity=47.6027397260274, Blast_Score=248, Evalue=5e-66,
Organism=Drosophila melanogaster, GI17136816, Length=297, Percent_Identity=47.4747474747475, Blast_Score=241, Evalue=6e-64,
Organism=Drosophila melanogaster, GI17136818, Length=297, Percent_Identity=47.4747474747475, Blast_Score=240, Evalue=7e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_ERYLH (Q2N7E3)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_459195.1
- ProteinModelPortal:   Q2N7E3
- SMR:   Q2N7E3
- STRING:   Q2N7E3
- GeneID:   3870415
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_11530
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   CLMMLRD
- PhylomeDB:   Q2N7E3
- ProtClustDB:   PRK14188
- BioCyc:   ELIT314225:ELI_11530-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31372; Mature: 31240

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAEIIDGKAFAARLRERVGALAEKFEAAAGRKPGLAVVLVGEDPASEVYVRNKGKATLA
CCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCEEEE
ANMESFEYKLPADTTAQDLLALVKKLNGDPAVDGILVQLPLPDHLDEQSIIAAISPDKDV
ECCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCC
DGFHVINAGRLSVGQRGFVPCTPLGCMMLLADRLGDLSGLEAVVIGRSNIVGKPMAQLLL
CCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHH
DANATVTIAHSRTRNLPEVVKRADIVVAAVGRAEMVKPEWLKDGATVIDVGINRLPPEPG
CCCCEEEEECCCCCCHHHHHHHHCEEEEECCCHHCCCCHHHCCCCEEEEECCCCCCCCCC
KERGRLVGDVDFGRASEVAAAITPVPGGVGPMTIAVLLRNTLVAAHRNEGIDLPEDAI
HHHCCEEECCCCCCHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TAEIIDGKAFAARLRERVGALAEKFEAAAGRKPGLAVVLVGEDPASEVYVRNKGKATLA
CCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCEEEE
ANMESFEYKLPADTTAQDLLALVKKLNGDPAVDGILVQLPLPDHLDEQSIIAAISPDKDV
ECCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCC
DGFHVINAGRLSVGQRGFVPCTPLGCMMLLADRLGDLSGLEAVVIGRSNIVGKPMAQLLL
CCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHH
DANATVTIAHSRTRNLPEVVKRADIVVAAVGRAEMVKPEWLKDGATVIDVGINRLPPEPG
CCCCEEEEECCCCCCHHHHHHHHCEEEEECCCHHCCCCHHHCCCCEEEEECCCCCCCCCC
KERGRLVGDVDFGRASEVAAAITPVPGGVGPMTIAVLLRNTLVAAHRNEGIDLPEDAI
HHHCCEEECCCCCCHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA