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The map label for this gene is fadH [H]

Identifier: 85375119

GI number: 85375119

Start: 2315065

End: 2315964

Strand: Reverse

Name: fadH [H]

Synonym: ELI_11460

Alternate gene names: 85375119

Gene position: 2315964-2315065 (Counterclockwise)

Preceding gene: 85375124

Following gene: 85375118

Centisome position: 75.87

GC content: 62.33

Gene sequence:

>900_bases
ATGGATATTACCAAACTGATGTATCGCGATCGCTTGATGGAAGGCGAGCGTATCCTCGTCACCGGCGGTGGGACCGGGCT
GGGCAAGGAAATGACCGAGGCGTTTCTCAAGCTCGGCGCGGAGGTGCACATCTGCGGGCGGCGCGAAGGCAAGCTCACCG
AAACTGCCGACGAACTGATGAGCCGGCATGGCGGAACCGTCGTGGGCCATGCCTGCGACATTCGCGATCACGCCAATATC
ACCGCGATGATCGACACGATCTGGGAAGGCGGCGCGCTGACCGGTCTCGTCAACAATGCCGCGGGCAATTTCATCAGCCG
TACCGAAGACCTTTCTATCAACGGCTTCAACGCCATCGCCGATATCGTCTTTCGCGGCACCTTCACCATGACGCATGAAG
TCGGCACGCGGCTGATCAAGGAAGGCAAGCGCGGCAACTTCCTCTCGATCCTCACCACCTGGGTCTGGAACGGCGGGCCT
TTCGTGGTCCCCTCCGCCATGAGCAAGGCGGGCATCAACGTAATGACGCAAAGCCTCGCGACCGAATGGGGCCGCTACGG
CCTGCGCTTCAACGCCATCGCGCCGGGACTTTTTCCGACCGAGGGGATGAGCGCGCGGCTATCGCCCGGCGGACGCGGCG
ACAATTCTTCCGACCGGATGAACCCGATGGGGCGGCATGGCGAAATGCATGAGCTGGCCAATTTGGCCGTGTTCCTGATG
GGGCCGGGGGCGGAATGGGTGAACGGGCAGACCATCGCCATCGACGGGGCCGGCTATCAGGCCAATTCGGGCAATTTCTA
CCAGACACTGGCGCCGCTGGGCGACGAGGACTGGGCGAAGATGCGCGAGATGATCAAGGGCACCAACGCCAAGGACAAGG
CCGAGCGGACGACGGACTAA

Upstream 100 bases:

>100_bases
AATTGGACCCGAAGAGCGTATCATTCAAGGAAAGTCGCCAAGCCGGTTCGATCCCGCTATCGCAGCGAAATAAACGAACA
GCGGGTGAGGAGAGAACGCC

Downstream 100 bases:

>100_bases
CGCCGTGGCACAGGTAACGATGGAGCCCTGGGCGAAAGCGCAGGAGGTACTGGCCGACGGCGGGGTGGCGTTCACCGCCT
CGCGCGATGGCGAGACCTGG

Product: putative 2, 4-dienoyl-CoA reductase (NADPH)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MDITKLMYRDRLMEGERILVTGGGTGLGKEMTEAFLKLGAEVHICGRREGKLTETADELMSRHGGTVVGHACDIRDHANI
TAMIDTIWEGGALTGLVNNAAGNFISRTEDLSINGFNAIADIVFRGTFTMTHEVGTRLIKEGKRGNFLSILTTWVWNGGP
FVVPSAMSKAGINVMTQSLATEWGRYGLRFNAIAPGLFPTEGMSARLSPGGRGDNSSDRMNPMGRHGEMHELANLAVFLM
GPGAEWVNGQTIAIDGAGYQANSGNFYQTLAPLGDEDWAKMREMIKGTNAKDKAERTTD

Sequences:

>Translated_299_residues
MDITKLMYRDRLMEGERILVTGGGTGLGKEMTEAFLKLGAEVHICGRREGKLTETADELMSRHGGTVVGHACDIRDHANI
TAMIDTIWEGGALTGLVNNAAGNFISRTEDLSINGFNAIADIVFRGTFTMTHEVGTRLIKEGKRGNFLSILTTWVWNGGP
FVVPSAMSKAGINVMTQSLATEWGRYGLRFNAIAPGLFPTEGMSARLSPGGRGDNSSDRMNPMGRHGEMHELANLAVFLM
GPGAEWVNGQTIAIDGAGYQANSGNFYQTLAPLGDEDWAKMREMIKGTNAKDKAERTTD
>Mature_299_residues
MDITKLMYRDRLMEGERILVTGGGTGLGKEMTEAFLKLGAEVHICGRREGKLTETADELMSRHGGTVVGHACDIRDHANI
TAMIDTIWEGGALTGLVNNAAGNFISRTEDLSINGFNAIADIVFRGTFTMTHEVGTRLIKEGKRGNFLSILTTWVWNGGP
FVVPSAMSKAGINVMTQSLATEWGRYGLRFNAIAPGLFPTEGMSARLSPGGRGDNSSDRMNPMGRHGEMHELANLAVFLM
GPGAEWVNGQTIAIDGAGYQANSGNFYQTLAPLGDEDWAKMREMIKGTNAKDKAERTTD

Specific function: Auxiliary enzyme of beta-oxidation. It participates in the metabolism of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3- enoyl-CoA

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2,4-dienoyl-CoA reductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503301, Length=253, Percent_Identity=42.6877470355731, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI19923817, Length=252, Percent_Identity=31.3492063492063, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI10190704, Length=256, Percent_Identity=28.515625, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI59889578, Length=211, Percent_Identity=30.3317535545024, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI15277342, Length=248, Percent_Identity=26.6129032258064, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI7705925, Length=246, Percent_Identity=23.1707317073171, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI20149619, Length=189, Percent_Identity=26.984126984127, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1789208, Length=248, Percent_Identity=30.241935483871, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1787905, Length=249, Percent_Identity=28.1124497991968, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082160, Length=248, Percent_Identity=26.6129032258064, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1790717, Length=245, Percent_Identity=26.9387755102041, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082100, Length=261, Percent_Identity=29.5019157088123, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI2367175, Length=243, Percent_Identity=28.8065843621399, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787335, Length=252, Percent_Identity=28.968253968254, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788459, Length=250, Percent_Identity=25.6, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI2367365, Length=247, Percent_Identity=27.5303643724696, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17536025, Length=292, Percent_Identity=42.1232876712329, Blast_Score=211, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17536651, Length=293, Percent_Identity=41.6382252559727, Blast_Score=205, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17561272, Length=274, Percent_Identity=42.3357664233577, Blast_Score=197, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI17560150, Length=256, Percent_Identity=27.734375, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17562906, Length=263, Percent_Identity=29.6577946768061, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17562990, Length=261, Percent_Identity=27.2030651340996, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI115534694, Length=250, Percent_Identity=27.6, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17562908, Length=259, Percent_Identity=28.957528957529, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI72000259, Length=259, Percent_Identity=26.2548262548263, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17560220, Length=262, Percent_Identity=26.3358778625954, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17531453, Length=260, Percent_Identity=28.4615384615385, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193204405, Length=261, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17563726, Length=258, Percent_Identity=27.1317829457364, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17538182, Length=259, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=267, Percent_Identity=25.0936329588015, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25147288, Length=250, Percent_Identity=26.8, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17555706, Length=255, Percent_Identity=23.921568627451, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17551412, Length=260, Percent_Identity=26.9230769230769, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71982365, Length=195, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=259, Percent_Identity=29.7297297297297, Blast_Score=111, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21357041, Length=257, Percent_Identity=27.6264591439689, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28571526, Length=257, Percent_Identity=26.8482490272374, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24644339, Length=251, Percent_Identity=26.2948207171315, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24639444, Length=252, Percent_Identity=25, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24643142, Length=258, Percent_Identity=25.968992248062, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI23397609, Length=256, Percent_Identity=27.734375, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.34 [H]

Molecular weight: Translated: 32347; Mature: 32347

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDITKLMYRDRLMEGERILVTGGGTGLGKEMTEAFLKLGAEVHICGRREGKLTETADELM
CCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHH
SRHGGTVVGHACDIRDHANITAMIDTIWEGGALTGLVNNAAGNFISRTEDLSINGFNAIA
HHCCCEEEEEEECCCCCCCEEEEEEHHHCCCCCEEEHHHHCCCHHCCCCCCCCCCHHHHH
DIVFRGTFTMTHEVGTRLIKEGKRGNFLSILTTWVWNGGPFVVPSAMSKAGINVMTQSLA
HHHHHCCEEEHHHHHHHHHHCCCCCCHHEEEHHHHCCCCCEEECCHHHHCCHHHHHHHHH
TEWGRYGLRFNAIAPGLFPTEGMSARLSPGGRGDNSSDRMNPMGRHGEMHELANLAVFLM
HHHHHCCEEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHCEEEEEE
GPGAEWVNGQTIAIDGAGYQANSGNFYQTLAPLGDEDWAKMREMIKGTNAKDKAERTTD
CCCCCCCCCEEEEEECCCEECCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCHHCCCC
>Mature Secondary Structure
MDITKLMYRDRLMEGERILVTGGGTGLGKEMTEAFLKLGAEVHICGRREGKLTETADELM
CCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHH
SRHGGTVVGHACDIRDHANITAMIDTIWEGGALTGLVNNAAGNFISRTEDLSINGFNAIA
HHCCCEEEEEEECCCCCCCEEEEEEHHHCCCCCEEEHHHHCCCHHCCCCCCCCCCHHHHH
DIVFRGTFTMTHEVGTRLIKEGKRGNFLSILTTWVWNGGPFVVPSAMSKAGINVMTQSLA
HHHHHCCEEEHHHHHHHHHHCCCCCCHHEEEHHHHCCCCCEEECCHHHHCCHHHHHHHHH
TEWGRYGLRFNAIAPGLFPTEGMSARLSPGGRGDNSSDRMNPMGRHGEMHELANLAVFLM
HHHHHCCEEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHCEEEEEE
GPGAEWVNGQTIAIDGAGYQANSGNFYQTLAPLGDEDWAKMREMIKGTNAKDKAERTTD
CCCCCCCCCEEEEEECCCEECCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]