Click here to switch to the map view.

The map label for this gene is cheB2 [H]

Identifier: 85375077

GI number: 85375077

Start: 2275463

End: 2276491

Strand: Reverse

Name: cheB2 [H]

Synonym: ELI_11250

Alternate gene names: 85375077

Gene position: 2276491-2275463 (Counterclockwise)

Preceding gene: 85375078

Following gene: 85375076

Centisome position: 74.58

GC content: 66.86

Gene sequence:

>1029_bases
ATGGCGGTCCGGGTCATGATCGTGGACGATTCGATCACCGCGCGGGCTGCGTTGGCGAGGGCCATCGACAGCAGCGGCAC
GGCAAAGGTCGTGGGCACTGCCAGCAGCGCCGAAGTCGCCCTGCAATTGCTGCGCGAGCAGCGGGTCGATGTCATCCTGC
TCGATCTCGAAATGCCTGGCATGGGCGGTCTCGAGGCCCTGCCGCGCCTGATCGAGGCGTCTGCGGGGGCGCAAATTCTC
GTCGTCTCCAGTCTCACCACTGCCGGAGCCGAGCCGACACTGGCCGCCATGGCGATGGGCGCTGCCGATACCCTGGCGAA
ACCCCGGGCCGGAGACTTCAACGAAGCCTACCGGGCCGAGCTGCTCGCCAAGCTGCTAGCGCTCGCTGGAACTGCCGATA
CCGCCGATTTGCCATCTAGCCCGGCGGTCCGGCCCGCCACCCGCACCATCAGGCCGGAATTGATCGCGCTAGGTGCATCG
ACCGGCGGAATCCACGCGCTCGGGCGCTTCTTCGCCGCGCTTCCACAGTCTGTCACTGTGCCGATGGTGTTGACGCAACA
TCTGCCTCACAGCTTCATGCCGGTATTCGCACGGCAGGTGAGCGCCATGGCCAAACGGCCCGCCGCAATCGCCGCCGACG
GCGAACTTATCCGGCCGAATACCGTCATGATCGCTCCGGGACATGCCCATCTGGAAGTGCGCAGAAGCGGCGGTGCGCTG
ACCTGTGCCCTCGTCAAATTCCCCGCCCCCAGCGGATGCTGCCCTTCCGTCGATCCCATGTTCGAATCCGTCGCTAATGT
CGTTGGCCACGCCGGGCTCGGCATCGTCCTGTCCGGTATGGGCCGCGACGGTGCTTTGGGCGCGCTGCAACTCGATCGGG
CGGGAGGGACCGTGCTCGCCCAGAACGAGGACAGTTGCGCGGTGTTCGGTATGCCGCGCGGCGTCGTCGAAGCCGGCATC
GCGCGCGCGATCGAGCCGCCGGAAGCGCTGGCACGCTATGTGGCGAACCTCGCAGGGGTCGACGCATGA

Upstream 100 bases:

>100_bases
AGATCAAATTGCAACTGGTGGGTTGCGCCTGAGGAGGTCATCGATGCGCTCTGCGCTCGCCCCCGATACGCCCGATACCC
CCCGCATCCCGGGTCGTCGC

Downstream 100 bases:

>100_bases
GCGACAATCCCGCCTCGCATCAGATCATCGCCGATCTGCTTCGCGCGCGGACCGGGCAACATCTCGGGCCCGACCGGATG
TGGCGCATCTCGGGCGCGCT

Product: protein-glutamate methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MAVRVMIVDDSITARAALARAIDSSGTAKVVGTASSAEVALQLLREQRVDVILLDLEMPGMGGLEALPRLIEASAGAQIL
VVSSLTTAGAEPTLAAMAMGAADTLAKPRAGDFNEAYRAELLAKLLALAGTADTADLPSSPAVRPATRTIRPELIALGAS
TGGIHALGRFFAALPQSVTVPMVLTQHLPHSFMPVFARQVSAMAKRPAAIAADGELIRPNTVMIAPGHAHLEVRRSGGAL
TCALVKFPAPSGCCPSVDPMFESVANVVGHAGLGIVLSGMGRDGALGALQLDRAGGTVLAQNEDSCAVFGMPRGVVEAGI
ARAIEPPEALARYVANLAGVDA

Sequences:

>Translated_342_residues
MAVRVMIVDDSITARAALARAIDSSGTAKVVGTASSAEVALQLLREQRVDVILLDLEMPGMGGLEALPRLIEASAGAQIL
VVSSLTTAGAEPTLAAMAMGAADTLAKPRAGDFNEAYRAELLAKLLALAGTADTADLPSSPAVRPATRTIRPELIALGAS
TGGIHALGRFFAALPQSVTVPMVLTQHLPHSFMPVFARQVSAMAKRPAAIAADGELIRPNTVMIAPGHAHLEVRRSGGAL
TCALVKFPAPSGCCPSVDPMFESVANVVGHAGLGIVLSGMGRDGALGALQLDRAGGTVLAQNEDSCAVFGMPRGVVEAGI
ARAIEPPEALARYVANLAGVDA
>Mature_341_residues
AVRVMIVDDSITARAALARAIDSSGTAKVVGTASSAEVALQLLREQRVDVILLDLEMPGMGGLEALPRLIEASAGAQILV
VSSLTTAGAEPTLAAMAMGAADTLAKPRAGDFNEAYRAELLAKLLALAGTADTADLPSSPAVRPATRTIRPELIALGAST
GGIHALGRFFAALPQSVTVPMVLTQHLPHSFMPVFARQVSAMAKRPAAIAADGELIRPNTVMIAPGHAHLEVRRSGGALT
CALVKFPAPSGCCPSVDPMFESVANVVGHAGLGIVLSGMGRDGALGALQLDRAGGTVLAQNEDSCAVFGMPRGVVEAGIA
RAIEPPEALARYVANLAGVDA

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR [H]

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=324, Percent_Identity=38.5802469135802, Blast_Score=187, Evalue=8e-49,
Organism=Escherichia coli, GI1788222, Length=107, Percent_Identity=38.3177570093458, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789 [H]

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg [H]

EC number: =3.1.1.61 [H]

Molecular weight: Translated: 34871; Mature: 34740

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50122 CHEB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVRVMIVDDSITARAALARAIDSSGTAKVVGTASSAEVALQLLREQRVDVILLDLEMPG
CEEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCC
MGGLEALPRLIEASAGAQILVVSSLTTAGAEPTLAAMAMGAADTLAKPRAGDFNEAYRAE
CCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHCCHHHHCCCCCCCCHHHHHHH
LLAKLLALAGTADTADLPSSPAVRPATRTIRPELIALGASTGGIHALGRFFAALPQSVTV
HHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCH
PMVLTQHLPHSFMPVFARQVSAMAKRPAAIAADGELIRPNTVMIAPGHAHLEVRRSGGAL
HHHHHHHCCHHHHHHHHHHHHHHHHCCCEEECCCCEECCCEEEEECCCCEEEEECCCCEE
TCALVKFPAPSGCCPSVDPMFESVANVVGHAGLGIVLSGMGRDGALGALQLDRAGGTVLA
EEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCCEEEE
QNEDSCAVFGMPRGVVEAGIARAIEPPEALARYVANLAGVDA
ECCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AVRVMIVDDSITARAALARAIDSSGTAKVVGTASSAEVALQLLREQRVDVILLDLEMPG
EEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCC
MGGLEALPRLIEASAGAQILVVSSLTTAGAEPTLAAMAMGAADTLAKPRAGDFNEAYRAE
CCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHCCHHHHCCCCCCCCHHHHHHH
LLAKLLALAGTADTADLPSSPAVRPATRTIRPELIALGASTGGIHALGRFFAALPQSVTV
HHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCH
PMVLTQHLPHSFMPVFARQVSAMAKRPAAIAADGELIRPNTVMIAPGHAHLEVRRSGGAL
HHHHHHHCCHHHHHHHHHHHHHHHHCCCEEECCCCEECCCEEEEECCCCEEEEECCCCEE
TCALVKFPAPSGCCPSVDPMFESVANVVGHAGLGIVLSGMGRDGALGALQLDRAGGTVLA
EEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCCEEEE
QNEDSCAVFGMPRGVVEAGIARAIEPPEALARYVANLAGVDA
ECCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA