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The map label for this gene is priA [H]

Identifier: 85374570

GI number: 85374570

Start: 1775146

End: 1777311

Strand: Direct

Name: priA [H]

Synonym: ELI_08715

Alternate gene names: 85374570

Gene position: 1775146-1777311 (Clockwise)

Preceding gene: 85374562

Following gene: 85374571

Centisome position: 58.16

GC content: 66.85

Gene sequence:

>2166_bases
ATGAACCGCGCGCGCATCCTCACATTCAACGCCGCCCTGCCGGTGCTCGATTACCGGGTGCCGGAGGGGATGAGCGTGGT
GCCGGGCAGCGTCGTGGTCGCCCCGCTCGGCCCGCGCGAAATCCTCGGCGTCGTGTGGGAGGAAGAACGCCTGCCGGGTC
AATCGGTGCCCGATGCCAAGCTTCGCCCCTTGCGTGATACGGTGCCGGTGCCGCCCCTGCGCCCGGAATTGCGGCGGCTG
ATCGAATGGACGGCGGATTATTACTGCGCCCCGATGAGTGCTGTGGCGCGGATGGTGCTGTCGAGCGGCGGCGCTCTGCG
CGGGCCGATGACAACGACGGAATATCGCCTCTCGGGCGGCATGCCGGAGCGCATGACCGCGCAGCGCGAGAGGGCGATGG
AGCGGCTGGAAGGCGAACAGGCGACCATCCGCGAACTGGCCGAGATCGCCGAGGTGTCCGACGGCGTGCTACGGGGCCTC
GTGAACCAGGGTGTGCTGGAGCCGGTCGTGGTCGATATCGACCGGTCCTACCCCCGCGCGCATCCCGACCATGCCGAACC
GATCCTGTCGGCGGAGCAGAAGGAGGTAGCAGACGAGCTGGTGGCCGCCGTCGAGGCAGAAGAGTTCGCCCCGATCCTGC
TCGACGGCGTGACCGGTTCGGGCAAGACCGAGACCTATTTCGAGCCCGTCGCGGCGGCGTTGCGGATGGACCGGCAGGTG
CTTGTCCTTTTGCCCGAGATCGCGCTCACGGAAGCCTTTCTGCGCCGCTTCGCAGATCGCTTCGGTGCTGCGCCGATCGT
CTGGCATTCGAGCCTGAAGTCGACCGAGCGCCGCCGCGCGTGGCGGGCGATCTCGACGGGCGAGGCACAGGTGGTCGTCG
GCGCACGCTCGGCGCTGTTCCTGCCATATGACAATCTCGGCCTGATCGTGGTCGATGAAGCGCATGAGGTGAGCTTCAAG
CAGGACGATGGCGTGCGCTACAATGCCCGCGACGTCGCGGTAATGCGCGGACATTTCGAGAAGCATCCCGTGATTCTCGC
CAGCGCCACTCCCGCGCTCGAAAGCCTGCAGATGGCCGAAAGCGGCATCTACCGGAAGCTGGACCTGCCGAGCCGGTATG
GCGGGGCGGAGCTGCCGGACATAAAGCTGATAGACCTCACGGAAGAAAAGCCCGAGCGCGGTCGCTGGCTCGCCCCGTCG
CTCGCTCGCGAGCTGTTCGCGCGGCTGGAACGCGGCGAGCAGTCGCTGCTGTTCCTCAACCGCCGTGGTTATGCGCCCCT
GACGCTGTGCCGCAATTGCGGCTTCCGTTTCCAATGCAGCAATTGCAGCGCCTGGCTGGTCGAGCATCGTTTTTCGCAAC
GCCTAGCCTGCCACCATTGCGGGCACGAGGAACAGCCGCCCGAGACTTGCCCCGATTGTAACGAGCCCGATTGCCTAGTC
GCATGCGGACCCGGTGTGGAGCGGATTGCGGACGAGGTGGCGGAAATCCTGCCCGATGCCCGGGTCGCGGTGGTCACTTC
GGATACGCTCAACACGCCCGACAAGGCCGCCGAATTCGTAAGGCAGGCCGAAGCGCGAGAAATCGACGTGATAGTAGGCA
CGCAGCTCGTCACAAAGGGTTTCCATTTCCCAGAACTGACGCTTGTCGGCGTGGTCGATGCCGACCTAGGCCTGGAAGGC
GGCGATCTGCGCGCGGCCGAGCGAACCTACCAGCAGGTCGCGCAAGTCGCCGGGCGCGCCGGGCGTGGCTCGAAGCCGGG
CGAAGTCCTGATCCAGACCCGCCACCCCGAAGCGCCGGTAATCGAAGCGCTTGCCGCCGGAGATCGCGACGCCTTCTACG
AAGCCGAAACCGAAGCGCGGCGTTTCGCCGGGGCCCCGCCCTTCGGACGCTGGGCCGCGATCATCGTCTCTTCTGAAGAC
GAGGCCGAAGCGCGCGAAGCCGCGCAGCGCATCGGCGCGACCCAGCCGCGGATCGAGGATATCGCCATCCTCGGCCCCGC
CCCTGCCCCGATGGCGCTGCTGCGCGGTCGCTATCGCTACCGCTTCCTCGTCAACGCGCGCCGCAGCGCAAAGGTGCAGG
AGGCGCTGCGCCAATGGCTCGATCCGCTGGACTTCCCGCGCGGGGTGCGCGTATCGATCGACATCGATCCCTACAGCTTC
GTTTGA

Upstream 100 bases:

>100_bases
CATCGAGCGACGGGTGATGATCTCGGACCCAACAATCTTGGACATAGTGCAGTCTCCAAACCTTGCGGGGCGTGCATGCC
GCCGCCCATATGGCACCAGC

Downstream 100 bases:

>100_bases
GAAGGACCTCCGCAATGCTCACCGTCCACCACCTGCGCCTGTCGCAATCCGAACGTATCGTCTGGCTGTGTGAGGAACTG
GGGCTCGATTACGACTTGAA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 721; Mature: 721

Protein sequence:

>721_residues
MNRARILTFNAALPVLDYRVPEGMSVVPGSVVVAPLGPREILGVVWEEERLPGQSVPDAKLRPLRDTVPVPPLRPELRRL
IEWTADYYCAPMSAVARMVLSSGGALRGPMTTTEYRLSGGMPERMTAQRERAMERLEGEQATIRELAEIAEVSDGVLRGL
VNQGVLEPVVVDIDRSYPRAHPDHAEPILSAEQKEVADELVAAVEAEEFAPILLDGVTGSGKTETYFEPVAAALRMDRQV
LVLLPEIALTEAFLRRFADRFGAAPIVWHSSLKSTERRRAWRAISTGEAQVVVGARSALFLPYDNLGLIVVDEAHEVSFK
QDDGVRYNARDVAVMRGHFEKHPVILASATPALESLQMAESGIYRKLDLPSRYGGAELPDIKLIDLTEEKPERGRWLAPS
LARELFARLERGEQSLLFLNRRGYAPLTLCRNCGFRFQCSNCSAWLVEHRFSQRLACHHCGHEEQPPETCPDCNEPDCLV
ACGPGVERIADEVAEILPDARVAVVTSDTLNTPDKAAEFVRQAEAREIDVIVGTQLVTKGFHFPELTLVGVVDADLGLEG
GDLRAAERTYQQVAQVAGRAGRGSKPGEVLIQTRHPEAPVIEALAAGDRDAFYEAETEARRFAGAPPFGRWAAIIVSSED
EAEAREAAQRIGATQPRIEDIAILGPAPAPMALLRGRYRYRFLVNARRSAKVQEALRQWLDPLDFPRGVRVSIDIDPYSF
V

Sequences:

>Translated_721_residues
MNRARILTFNAALPVLDYRVPEGMSVVPGSVVVAPLGPREILGVVWEEERLPGQSVPDAKLRPLRDTVPVPPLRPELRRL
IEWTADYYCAPMSAVARMVLSSGGALRGPMTTTEYRLSGGMPERMTAQRERAMERLEGEQATIRELAEIAEVSDGVLRGL
VNQGVLEPVVVDIDRSYPRAHPDHAEPILSAEQKEVADELVAAVEAEEFAPILLDGVTGSGKTETYFEPVAAALRMDRQV
LVLLPEIALTEAFLRRFADRFGAAPIVWHSSLKSTERRRAWRAISTGEAQVVVGARSALFLPYDNLGLIVVDEAHEVSFK
QDDGVRYNARDVAVMRGHFEKHPVILASATPALESLQMAESGIYRKLDLPSRYGGAELPDIKLIDLTEEKPERGRWLAPS
LARELFARLERGEQSLLFLNRRGYAPLTLCRNCGFRFQCSNCSAWLVEHRFSQRLACHHCGHEEQPPETCPDCNEPDCLV
ACGPGVERIADEVAEILPDARVAVVTSDTLNTPDKAAEFVRQAEAREIDVIVGTQLVTKGFHFPELTLVGVVDADLGLEG
GDLRAAERTYQQVAQVAGRAGRGSKPGEVLIQTRHPEAPVIEALAAGDRDAFYEAETEARRFAGAPPFGRWAAIIVSSED
EAEAREAAQRIGATQPRIEDIAILGPAPAPMALLRGRYRYRFLVNARRSAKVQEALRQWLDPLDFPRGVRVSIDIDPYSF
V
>Mature_721_residues
MNRARILTFNAALPVLDYRVPEGMSVVPGSVVVAPLGPREILGVVWEEERLPGQSVPDAKLRPLRDTVPVPPLRPELRRL
IEWTADYYCAPMSAVARMVLSSGGALRGPMTTTEYRLSGGMPERMTAQRERAMERLEGEQATIRELAEIAEVSDGVLRGL
VNQGVLEPVVVDIDRSYPRAHPDHAEPILSAEQKEVADELVAAVEAEEFAPILLDGVTGSGKTETYFEPVAAALRMDRQV
LVLLPEIALTEAFLRRFADRFGAAPIVWHSSLKSTERRRAWRAISTGEAQVVVGARSALFLPYDNLGLIVVDEAHEVSFK
QDDGVRYNARDVAVMRGHFEKHPVILASATPALESLQMAESGIYRKLDLPSRYGGAELPDIKLIDLTEEKPERGRWLAPS
LARELFARLERGEQSLLFLNRRGYAPLTLCRNCGFRFQCSNCSAWLVEHRFSQRLACHHCGHEEQPPETCPDCNEPDCLV
ACGPGVERIADEVAEILPDARVAVVTSDTLNTPDKAAEFVRQAEAREIDVIVGTQLVTKGFHFPELTLVGVVDADLGLEG
GDLRAAERTYQQVAQVAGRAGRGSKPGEVLIQTRHPEAPVIEALAAGDRDAFYEAETEARRFAGAPPFGRWAAIIVSSED
EAEAREAAQRIGATQPRIEDIAILGPAPAPMALLRGRYRYRFLVNARRSAKVQEALRQWLDPLDFPRGVRVSIDIDPYSF
V

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=744, Percent_Identity=34.5430107526882, Blast_Score=383, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 79566; Mature: 79566

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00690 DEAH_ATP_HELICASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRARILTFNAALPVLDYRVPEGMSVVPGSVVVAPLGPREILGVVWEEERLPGQSVPDAK
CCCCEEEEEECCCCHHCCCCCCCCEECCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCC
LRPLRDTVPVPPLRPELRRLIEWTADYYCAPMSAVARMVLSSGGALRGPMTTTEYRLSGG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEECCC
MPERMTAQRERAMERLEGEQATIRELAEIAEVSDGVLRGLVNQGVLEPVVVDIDRSYPRA
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
HPDHAEPILSAEQKEVADELVAAVEAEEFAPILLDGVTGSGKTETYFEPVAAALRMDRQV
CCCCCCHHHCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHCCCEE
LVLLPEIALTEAFLRRFADRFGAAPIVWHSSLKSTERRRAWRAISTGEAQVVVGARSALF
EEEECHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHCCCCCEEEEEECCCEEE
LPYDNLGLIVVDEAHEVSFKQDDGVRYNARDVAVMRGHFEKHPVILASATPALESLQMAE
EEECCCCEEEEECCCCCCCCCCCCCEECCCHHHHHHCCCCCCCEEEEECCHHHHHHHHHH
SGIYRKLDLPSRYGGAELPDIKLIDLTEEKPERGRWLAPSLARELFARLERGEQSLLFLN
HCCEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEE
RRGYAPLTLCRNCGFRFQCSNCSAWLVEHRFSQRLACHHCGHEEQPPETCPDCNEPDCLV
CCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEE
ACGPGVERIADEVAEILPDARVAVVTSDTLNTPDKAAEFVRQAEAREIDVIVGTQLVTKG
EECCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECHHHHHCC
FHFPELTLVGVVDADLGLEGGDLRAAERTYQQVAQVAGRAGRGSKPGEVLIQTRHPEAPV
CCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHH
IEALAAGDRDAFYEAETEARRFAGAPPFGRWAAIIVSSEDEAEAREAAQRIGATQPRIED
HHHHHCCCCHHHEHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCC
IAILGPAPAPMALLRGRYRYRFLVNARRSAKVQEALRQWLDPLDFPRGVRVSIDIDPYSF
EEEECCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCC
V
C
>Mature Secondary Structure
MNRARILTFNAALPVLDYRVPEGMSVVPGSVVVAPLGPREILGVVWEEERLPGQSVPDAK
CCCCEEEEEECCCCHHCCCCCCCCEECCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCC
LRPLRDTVPVPPLRPELRRLIEWTADYYCAPMSAVARMVLSSGGALRGPMTTTEYRLSGG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEECCC
MPERMTAQRERAMERLEGEQATIRELAEIAEVSDGVLRGLVNQGVLEPVVVDIDRSYPRA
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
HPDHAEPILSAEQKEVADELVAAVEAEEFAPILLDGVTGSGKTETYFEPVAAALRMDRQV
CCCCCCHHHCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHCCCEE
LVLLPEIALTEAFLRRFADRFGAAPIVWHSSLKSTERRRAWRAISTGEAQVVVGARSALF
EEEECHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHCCCCCEEEEEECCCEEE
LPYDNLGLIVVDEAHEVSFKQDDGVRYNARDVAVMRGHFEKHPVILASATPALESLQMAE
EEECCCCEEEEECCCCCCCCCCCCCEECCCHHHHHHCCCCCCCEEEEECCHHHHHHHHHH
SGIYRKLDLPSRYGGAELPDIKLIDLTEEKPERGRWLAPSLARELFARLERGEQSLLFLN
HCCEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEE
RRGYAPLTLCRNCGFRFQCSNCSAWLVEHRFSQRLACHHCGHEEQPPETCPDCNEPDCLV
CCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEE
ACGPGVERIADEVAEILPDARVAVVTSDTLNTPDKAAEFVRQAEAREIDVIVGTQLVTKG
EECCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECHHHHHCC
FHFPELTLVGVVDADLGLEGGDLRAAERTYQQVAQVAGRAGRGSKPGEVLIQTRHPEAPV
CCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHH
IEALAAGDRDAFYEAETEARRFAGAPPFGRWAAIIVSSEDEAEAREAAQRIGATQPRIED
HHHHHCCCCHHHEHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCC
IAILGPAPAPMALLRGRYRYRFLVNARRSAKVQEALRQWLDPLDFPRGVRVSIDIDPYSF
EEEECCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2861810 [H]