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The map label for this gene is hprA [H]

Identifier: 85374561

GI number: 85374561

Start: 1766823

End: 1767761

Strand: Reverse

Name: hprA [H]

Synonym: ELI_08670

Alternate gene names: 85374561

Gene position: 1767761-1766823 (Counterclockwise)

Preceding gene: 85374563

Following gene: 85374559

Centisome position: 57.91

GC content: 63.37

Gene sequence:

>939_bases
ATGCCCATCGCCGTCCTGTCCGCCCTCGTCCGCCCGCTCGTCGAGCCGCATCTTCCCGACGGGATAGAAGCGAAGTTCTT
CGCCTCTGTCGAGGATTTGATGGAGCTGGCACCGCAGGCGGAAATCGGCTGGTTCGATCTCGACCAGAAGCAGCCGATGA
TCGAAGCGGTGAAAAGGGCCGAGAGGCTGAAATGGCTGAGCTCGATCTATGCCGGGCTCGATTTCCTGCCGCACGAATTG
TTGCTGGAGCGCGGCGTGACGGTCACCAACGGCGTCGGGATCAACGCCGTCACCATTGCGGAGTATGTCGTGATGGGGAT
GCTGGTCCACGCCAAGGGCTATCGCGATGTGGTGCGGGCACAGGAGAAGCATGAGTGGCTGCTCGATTCGCCCGGCAAGA
TCGAACTGGCCGGTTCCAGGGCGCTATTGCTCGGCTACGGCGCGATCGGACAGCTGATCAAACCGAGGCTCGAGGCGTTC
GATATCGAGGTCACCGTGGTGCGGCGCTCGGGCGGGGAGGGCTGCCTGGCACCCGACCAGTGGCGCGGCCAACTCGGCGA
ATTCGACTGGATCATCCTCGCCGTCCCCGCCACCGCAGAAACCGAAGGCATGATCGGTGCCGAGGAGCTGGCGGCAATGA
AAAGCGACGCGGTCTTGGTGAACATCGCCCGCGGCGAAGTGGTCGACCAGCCCGCGCTGGTAAAGGCGCTGCAAGACAAG
ACGATCGGCGGCGCGTTTCTCGATGTGACCACGCCCGAGCCGCTGCCCGCCGACCACGCGCTCTGGTCGCTCGACAATGC
GCATGTCACCATGCACCTGTCGGGTCGCGCGCAGACCAAGATGTTTCAGCGCTCGGCCGAGCGGTTTGTGCAGAATTGTC
ATCGCTACATCGCAGGCGAACCAGTCGAACCGCGTTTCGACCTGACGCTGGGTTATTAG

Upstream 100 bases:

>100_bases
TGATGACGTGCGCAGGCGTGTGCGGTGCGTTCATCGACTGGCATCCCGGAAGCCGATCGGGCAAGGGAAGGGCTTCCGCG
CCACTAGCCAGAGATTGTCC

Downstream 100 bases:

>100_bases
AGTAAGGAACCACTCCTGACCCGTTCGTGCTGAGCCTGTCGAAGCACTGTTCTTCTTTTGGCAAGCGCTGCGCTCGAAGA
AAGAGCGGCCCTTCGACAGG

Product: putative dehydrogenase

Products: NA

Alternate protein names: GDH; Glyoxylate reductase; Hydroxypyruvate dehydrogenase; NADH-dependent hydroxypyruvate reductase; HPR; HPR-A [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MPIAVLSALVRPLVEPHLPDGIEAKFFASVEDLMELAPQAEIGWFDLDQKQPMIEAVKRAERLKWLSSIYAGLDFLPHEL
LLERGVTVTNGVGINAVTIAEYVVMGMLVHAKGYRDVVRAQEKHEWLLDSPGKIELAGSRALLLGYGAIGQLIKPRLEAF
DIEVTVVRRSGGEGCLAPDQWRGQLGEFDWIILAVPATAETEGMIGAEELAAMKSDAVLVNIARGEVVDQPALVKALQDK
TIGGAFLDVTTPEPLPADHALWSLDNAHVTMHLSGRAQTKMFQRSAERFVQNCHRYIAGEPVEPRFDLTLGY

Sequences:

>Translated_312_residues
MPIAVLSALVRPLVEPHLPDGIEAKFFASVEDLMELAPQAEIGWFDLDQKQPMIEAVKRAERLKWLSSIYAGLDFLPHEL
LLERGVTVTNGVGINAVTIAEYVVMGMLVHAKGYRDVVRAQEKHEWLLDSPGKIELAGSRALLLGYGAIGQLIKPRLEAF
DIEVTVVRRSGGEGCLAPDQWRGQLGEFDWIILAVPATAETEGMIGAEELAAMKSDAVLVNIARGEVVDQPALVKALQDK
TIGGAFLDVTTPEPLPADHALWSLDNAHVTMHLSGRAQTKMFQRSAERFVQNCHRYIAGEPVEPRFDLTLGY
>Mature_311_residues
PIAVLSALVRPLVEPHLPDGIEAKFFASVEDLMELAPQAEIGWFDLDQKQPMIEAVKRAERLKWLSSIYAGLDFLPHELL
LERGVTVTNGVGINAVTIAEYVVMGMLVHAKGYRDVVRAQEKHEWLLDSPGKIELAGSRALLLGYGAIGQLIKPRLEAFD
IEVTVVRRSGGEGCLAPDQWRGQLGEFDWIILAVPATAETEGMIGAEELAAMKSDAVLVNIARGEVVDQPALVKALQDKT
IGGAFLDVTTPEPLPADHALWSLDNAHVTMHLSGRAQTKMFQRSAERFVQNCHRYIAGEPVEPRFDLTLGY

Specific function: Plays a central role in assimilation of carbon. It converts hydroxypyruvate to glycerate as a key step in the serine cycle, and may also play an important role in C2 reactions, by interconverting glyoxylate and glycolate [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=259, Percent_Identity=28.957528957529, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI23308577, Length=250, Percent_Identity=26.4, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1789279, Length=235, Percent_Identity=28.0851063829787, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87082289, Length=241, Percent_Identity=26.5560165975104, Blast_Score=75, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320925, Length=207, Percent_Identity=31.4009661835749, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322116, Length=207, Percent_Identity=30.9178743961353, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324964, Length=248, Percent_Identity=25, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321253, Length=174, Percent_Identity=28.735632183908, Blast_Score=66, Evalue=8e-12,
Organism=Drosophila melanogaster, GI28574286, Length=251, Percent_Identity=29.8804780876494, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI45551003, Length=259, Percent_Identity=31.6602316602317, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24585514, Length=259, Percent_Identity=31.6602316602317, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI28574282, Length=259, Percent_Identity=31.6602316602317, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI28574284, Length=259, Percent_Identity=31.6602316602317, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI45552429, Length=259, Percent_Identity=31.6602316602317, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24585516, Length=226, Percent_Identity=31.858407079646, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI28571528, Length=176, Percent_Identity=38.0681818181818, Blast_Score=95, Evalue=7e-20,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.29 [H]

Molecular weight: Translated: 34266; Mature: 34135

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIAVLSALVRPLVEPHLPDGIEAKFFASVEDLMELAPQAEIGWFDLDQKQPMIEAVKRA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHH
ERLKWLSSIYAGLDFLPHELLLERGVTVTNGVGINAVTIAEYVVMGMLVHAKGYRDVVRA
HHHHHHHHHHHHHHHCHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
QEKHEWLLDSPGKIELAGSRALLLGYGAIGQLIKPRLEAFDIEVTVVRRSGGEGCLAPDQ
HHHCCHHCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCHH
WRGQLGEFDWIILAVPATAETEGMIGAEELAAMKSDAVLVNIARGEVVDQPALVKALQDK
HCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHCC
TIGGAFLDVTTPEPLPADHALWSLDNAHVTMHLSGRAQTKMFQRSAERFVQNCHRYIAGE
CCCCEEEECCCCCCCCCCHHHEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
PVEPRFDLTLGY
CCCCCCCEEECC
>Mature Secondary Structure 
PIAVLSALVRPLVEPHLPDGIEAKFFASVEDLMELAPQAEIGWFDLDQKQPMIEAVKRA
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHH
ERLKWLSSIYAGLDFLPHELLLERGVTVTNGVGINAVTIAEYVVMGMLVHAKGYRDVVRA
HHHHHHHHHHHHHHHCHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
QEKHEWLLDSPGKIELAGSRALLLGYGAIGQLIKPRLEAFDIEVTVVRRSGGEGCLAPDQ
HHHCCHHCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCHH
WRGQLGEFDWIILAVPATAETEGMIGAEELAAMKSDAVLVNIARGEVVDQPALVKALQDK
HCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHCC
TIGGAFLDVTTPEPLPADHALWSLDNAHVTMHLSGRAQTKMFQRSAERFVQNCHRYIAGE
CCCCEEEECCCCCCCCCCHHHEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
PVEPRFDLTLGY
CCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144463; 1729225; 1657886 [H]