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The map label for this gene is miaB

Identifier: 85374526

GI number: 85374526

Start: 1739472

End: 1740812

Strand: Direct

Name: miaB

Synonym: ELI_08495

Alternate gene names: 85374526

Gene position: 1739472-1740812 (Clockwise)

Preceding gene: 85374525

Following gene: 85374527

Centisome position: 56.99

GC content: 65.1

Gene sequence:

>1341_bases
ATGAAACCGACCCATTCTCCCAAGACCTACCGGGTCAAGAGCTTCGGCTGCCAGATGAACGTCTATGATGGCGAGCGCAT
GGCTGAGATGCTCGATGAAAAAGGCATCGAGCCTGCGCCCGAGGGCGAGGATGCGGACCTGGTCGTCCTCAACACCTGCC
ACATTCGCGAGAAAGCGGTCGACAAGGTCTATTCCGATATCGGCCGACTGACCAAAGGCAAGACGCAGACCAAGGCCCCC
ATGATCGCCGTCGCAGGCTGCGTCGCGCAGGCCGAGGGCGAAGAAATCATGGCGCGCGCCCCGGCGGTGTCGATGGTGGT
CGGGCCGCAGGCGTATCACCGCCTGCCCGGCATGATCGACGCAGCGGTCGCGGGCAAGCGCTCGACCGACACGGACATGC
CGGCAGACGCCAAGTTCGCCGCGCTACCCAAGCGTCGCAAGTCGGCCCCCAGCGCCTTCCTGACGATCCAGGAAGGCTGC
GACAAATTCTGCACCTATTGCGTGGTGCCCTACACCCGCGGCGCGGAAATCTCGCGGCCGTTCAGCGCCCTGATCGACGA
AGCGAAGAAACTGGTCGAAGCGGGCGCGAAGGAAATCACCCTGCTGGGGCAGAATGTCAGCGCGTGGACCGGTGAGGATG
CCAAGGGCCGGGCTCTCGGCATGGCAGGCCTGATCCGCGAGCTGGCCAAGGACCCCGACCTCAAGCGCGTCCGCTACACT
ACCAGCCACCCGGCCGACATGGATGACGAGCTGATCGCCACCCATGGAGAGGTCGAAAAGCTGATGCCCTATCTCCACCT
GCCGGTGCAGAGCGGCAACGATCGCGTGCTCAAGGCCATGAACCGCAGCCACACGGCGGAAAGCTATCTGCGGCTGCTGG
AGCGTTTCCGCGCCGCGCGGCCAGACCTCGCGCTATCCGGCGACTTCATCGTCGGCTTCCCCGGCGAGACCGAGGCCGAG
TTCGAGGACACGCTGAAGATCGTCGACGAAGTCCGCTACGCGCAGGCCTACAGTTTCAAATACTCCCCCCGCCCCGGCAC
CCCCGCTGCGACGATGGAACGTCAGGTCCCCAAAGAGGTGATGGACGAGCGGCTGCAGCGCCTGCAAGCCGCGCTCAACC
GCGACCAGGCGGCATTCAACGCTGGCAGCGTAGGGAGAACCTGCGAAGTCCTCGTCGAGCGCACGGGCAAGCATCCCGGC
CAGTGGCTCGGCAAATCGCCATGGCTGCAAAGCGTCTGGTTCGACGGAGACGTCGCGATCGGCGATCTCGTGCAGGTCGA
ACTGGTCGAAGCGGGACCGAACTCGCTGGCGGGGCAGCTGCTCGAAACCGTGGCCGCGTGA

Upstream 100 bases:

>100_bases
AATTCAATGTCGAGCCGATCCGCTACAGCGCGCCCGAATTTATCGAGTAGCCGCTCTGCTCTTGGAATGGCCGCGCGGTT
GGCCTAAAGGCGCGAGCCTC

Downstream 100 bases:

>100_bases
CAGCCGGATTACTTTCCCCAGCCCCGTTCGCCTCGCAAACTTGCACCCCGCCGCCATCGGCGTAAGCTTCCGACTCGAAG
CCAGCGAAAGGAACGCTCCG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKPTHSPKTYRVKSFGCQMNVYDGERMAEMLDEKGIEPAPEGEDADLVVLNTCHIREKAVDKVYSDIGRLTKGKTQTKAP
MIAVAGCVAQAEGEEIMARAPAVSMVVGPQAYHRLPGMIDAAVAGKRSTDTDMPADAKFAALPKRRKSAPSAFLTIQEGC
DKFCTYCVVPYTRGAEISRPFSALIDEAKKLVEAGAKEITLLGQNVSAWTGEDAKGRALGMAGLIRELAKDPDLKRVRYT
TSHPADMDDELIATHGEVEKLMPYLHLPVQSGNDRVLKAMNRSHTAESYLRLLERFRAARPDLALSGDFIVGFPGETEAE
FEDTLKIVDEVRYAQAYSFKYSPRPGTPAATMERQVPKEVMDERLQRLQAALNRDQAAFNAGSVGRTCEVLVERTGKHPG
QWLGKSPWLQSVWFDGDVAIGDLVQVELVEAGPNSLAGQLLETVAA

Sequences:

>Translated_446_residues
MKPTHSPKTYRVKSFGCQMNVYDGERMAEMLDEKGIEPAPEGEDADLVVLNTCHIREKAVDKVYSDIGRLTKGKTQTKAP
MIAVAGCVAQAEGEEIMARAPAVSMVVGPQAYHRLPGMIDAAVAGKRSTDTDMPADAKFAALPKRRKSAPSAFLTIQEGC
DKFCTYCVVPYTRGAEISRPFSALIDEAKKLVEAGAKEITLLGQNVSAWTGEDAKGRALGMAGLIRELAKDPDLKRVRYT
TSHPADMDDELIATHGEVEKLMPYLHLPVQSGNDRVLKAMNRSHTAESYLRLLERFRAARPDLALSGDFIVGFPGETEAE
FEDTLKIVDEVRYAQAYSFKYSPRPGTPAATMERQVPKEVMDERLQRLQAALNRDQAAFNAGSVGRTCEVLVERTGKHPG
QWLGKSPWLQSVWFDGDVAIGDLVQVELVEAGPNSLAGQLLETVAA
>Mature_446_residues
MKPTHSPKTYRVKSFGCQMNVYDGERMAEMLDEKGIEPAPEGEDADLVVLNTCHIREKAVDKVYSDIGRLTKGKTQTKAP
MIAVAGCVAQAEGEEIMARAPAVSMVVGPQAYHRLPGMIDAAVAGKRSTDTDMPADAKFAALPKRRKSAPSAFLTIQEGC
DKFCTYCVVPYTRGAEISRPFSALIDEAKKLVEAGAKEITLLGQNVSAWTGEDAKGRALGMAGLIRELAKDPDLKRVRYT
TSHPADMDDELIATHGEVEKLMPYLHLPVQSGNDRVLKAMNRSHTAESYLRLLERFRAARPDLALSGDFIVGFPGETEAE
FEDTLKIVDEVRYAQAYSFKYSPRPGTPAATMERQVPKEVMDERLQRLQAALNRDQAAFNAGSVGRTCEVLVERTGKHPG
QWLGKSPWLQSVWFDGDVAIGDLVQVELVEAGPNSLAGQLLETVAA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=477, Percent_Identity=34.5911949685535, Blast_Score=266, Evalue=4e-71,
Organism=Homo sapiens, GI28872782, Length=477, Percent_Identity=34.5911949685535, Blast_Score=265, Evalue=8e-71,
Organism=Homo sapiens, GI93277076, Length=443, Percent_Identity=28.4424379232506, Blast_Score=180, Evalue=2e-45,
Organism=Escherichia coli, GI1786882, Length=441, Percent_Identity=44.8979591836735, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1787057, Length=455, Percent_Identity=31.6483516483516, Blast_Score=202, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17553146, Length=460, Percent_Identity=31.9565217391304, Blast_Score=241, Evalue=6e-64,
Organism=Caenorhabditis elegans, GI71996771, Length=277, Percent_Identity=29.9638989169675, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI21356207, Length=472, Percent_Identity=32.8389830508475, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI19922432, Length=446, Percent_Identity=27.8026905829596, Blast_Score=156, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_ERYLH (Q2N950)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458588.1
- ProteinModelPortal:   Q2N950
- STRING:   Q2N950
- GeneID:   3871151
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_08495
- NMPDR:   fig|314225.3.peg.1343
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   Q2N950
- ProtClustDB:   PRK14325
- BioCyc:   ELIT314225:ELI_08495-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 48782; Mature: 48782

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPTHSPKTYRVKSFGCQMNVYDGERMAEMLDEKGIEPAPEGEDADLVVLNTCHIREKAV
CCCCCCCCEEEEEECCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHH
DKVYSDIGRLTKGKTQTKAPMIAVAGCVAQAEGEEIMARAPAVSMVVGPQAYHRLPGMID
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCEEEEECCHHHHHCCCHHH
AAVAGKRSTDTDMPADAKFAALPKRRKSAPSAFLTIQEGCDKFCTYCVVPYTRGAEISRP
HHHCCCCCCCCCCCCCCHHHHCCHHHCCCCHHEEEHHHHHHHHHHEEECCCCCCCCCCCH
FSALIDEAKKLVEAGAKEITLLGQNVSAWTGEDAKGRALGMAGLIRELAKDPDLKRVRYT
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEC
TSHPADMDDELIATHGEVEKLMPYLHLPVQSGNDRVLKAMNRSHTAESYLRLLERFRAAR
CCCCCCCCCHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCC
PDLALSGDFIVGFPGETEAEFEDTLKIVDEVRYAQAYSFKYSPRPGTPAATMERQVPKEV
CCCEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHCHHHH
MDERLQRLQAALNRDQAAFNAGSVGRTCEVLVERTGKHPGQWLGKSPWLQSVWFDGDVAI
HHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHEECCCCEEE
GDLVQVELVEAGPNSLAGQLLETVAA
CCEEEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKPTHSPKTYRVKSFGCQMNVYDGERMAEMLDEKGIEPAPEGEDADLVVLNTCHIREKAV
CCCCCCCCEEEEEECCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHH
DKVYSDIGRLTKGKTQTKAPMIAVAGCVAQAEGEEIMARAPAVSMVVGPQAYHRLPGMID
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCEEEEECCHHHHHCCCHHH
AAVAGKRSTDTDMPADAKFAALPKRRKSAPSAFLTIQEGCDKFCTYCVVPYTRGAEISRP
HHHCCCCCCCCCCCCCCHHHHCCHHHCCCCHHEEEHHHHHHHHHHEEECCCCCCCCCCCH
FSALIDEAKKLVEAGAKEITLLGQNVSAWTGEDAKGRALGMAGLIRELAKDPDLKRVRYT
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEC
TSHPADMDDELIATHGEVEKLMPYLHLPVQSGNDRVLKAMNRSHTAESYLRLLERFRAAR
CCCCCCCCCHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCC
PDLALSGDFIVGFPGETEAEFEDTLKIVDEVRYAQAYSFKYSPRPGTPAATMERQVPKEV
CCCEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHCHHHH
MDERLQRLQAALNRDQAAFNAGSVGRTCEVLVERTGKHPGQWLGKSPWLQSVWFDGDVAI
HHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHEECCCCEEE
GDLVQVELVEAGPNSLAGQLLETVAA
CCEEEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA