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The map label for this gene is nadE

Identifier: 85374508

GI number: 85374508

Start: 1719452

End: 1721770

Strand: Reverse

Name: nadE

Synonym: ELI_08405

Alternate gene names: 85374508

Gene position: 1721770-1719452 (Counterclockwise)

Preceding gene: 85374509

Following gene: 85374507

Centisome position: 56.41

GC content: 63.82

Gene sequence:

>2319_bases
ATGCATCAGGCAGGCGCCAAAATCCGCCGCTTTCGCGAAACCCACGATCCGCCGCTTTCGGCGGAGGAGTTCGGTGCGCG
CTATGGTGCGCCACAGCCATGGGCTAGCCGCACGGTTTATGGCTGGGAAGTCAAGGGCAAGGTCGCCCGCCCGCCCGTTC
AGAAACGGCTCGCCGCGCTCGGCGTCTGCGATCCCGGCGATTGGCTCGAAGAGGCCCGACCCGAAGACAACATCCCCACC
AGGAAAGGCACAGCCCGTATGAGCGACGCCGCACATCCCTTCTACGATCGCCACCGCCAAGGCATGATCCGCGTCGCCAC
GGCTACGCCGGCTGTTCGAACAGCTGACGTGCCCTTCAACCGCGACGGCATCATCGCCGAGGCGCGCAAAGCGCATGATC
TGGAGGTCGATCTCGTCGTCTATCCCGAACTGTGCGTCTCTTCCTACGCCATCGACGATCTGCACCTGCAGACCGCGATG
CTCGACGCGAGCGAAGCCGCAGTCGGCGCAATCGCGCAGGCCACGCGCGATCTCTCGCCCGTGCTCATCATCGGCGCGCC
GCTGAGACGGAACGGGAAAATCTACAATTGCGCGCTTGCCCTATCACGAGGACGCGTGCTGGGCGTGGTGCCCAAGAGCT
TCCTGCCCAATTACCGCGAATTCTACGAGAAGCGGCATTTCGCCCATGGCCGCAATTGCCAGGGGCTGGAGATTGCGCTG
AACGGTGAGAGAATGCCGTTCGGCACCGACCTGATCTTCGCCGCGCACGACCTGCCGGGATTCACCTTCGGCATCGAGAT
CTGCGAGGATTTCTGGTCGCCCAATCCGCCGGGCACGCTGGCGGCACTGGCAAGCGCGACGATCCTCGCCAATCTTTCCG
CCAGTCCGATCACCATCGGCCGCGCCGACGATCGCCACCTGCATTGCCGATCCAGCTCGGCCCGATCGATCTGCGCCTAC
GCCTATTCCGCCAGCGGTTTCGGCGAAAGCACGACCGACCTGTCGTGGGACGGGCAGGGCGTCGTCTATGAAATGGGCGA
GCTGATGAAGCAAAGCACGCGCTTCGATCTCGATACCGAGCTCTGCGCGGTCGATATCGATATCCAGCGTATCCTCGCCG
AACGCATGCGCAACCAGACCTTCAGCGACGCCGCCGAGCACGAGGGCCGACCCGAGGACTGGTATCGCCGCATAGAATTC
CAGCATGCCGGCACCGGCGGCGATACCGGCCTGATGCGCCCGATCCGCCGTTTCCCCTTCGTGCCCAACAACCGCGCGAC
GCTGGACGAAGATTGCTACGAGGCGTTCAACATCCAGGTCGATGCGCTGATGCGGCGGATCAAGGCGACCGGTCCCAAGA
GCCTCGTCATCGGCATTTCGGGCGGTCTCGACAGCACCCACGCGCTGATCGTCGCCGCCAAGGCCTGCGACCGGCTTGGC
CTGCCCCGCACGACCATTCGCGGCTACACCATGCCGGGCTTCGCGACGTCCGACTACACCAAGTCGAATGCATGGAAGCT
GATGCAAGCGATGGAGATCACCGCCGAGGAGATCGACATCAAGCCGACCGCGCGGACCATGCTGGAGAATATCGGCCACG
CCTTCGCCGATGGCGAACCGGTTTACGACACGACCTTCGAAAACGTGCAGGCGGGGCTGCGGACGGACTACCTGTTCCGG
CTCGCCGGCCAGCATAAGGGTTTCGTTGTCGGGACCGGAGACCTCAGCGAACTCGCGCTCGGCTGGTGCACCTATGGCGT
AGGCGACCACATGAGCCACTATGGCGTCAATGCGGGCGTGCCCAAGACGCTGATCCAGTACCTCATCCGCTGGACCATCG
AGACCGAGCAATTTGTGCCTGAATGCAGCGCGGTACTGGGCGCGATCCTCGATACCGAGATTTCGCCCGAACTGGTCCCG
GCGGGTGAGGACGGCGAGATACAAAGCACCGAAAGCACCATCGGGCCCTACGAGCTCAACGACTTCTTCCTTCACCACAT
CGTCCGCTACGGCCAGAGCCCGAGCAAGGTGGCGTTCCTCGCATGGCATGCCTGGAAGGATATCGAAGCGGGCGAATGGC
CCGAAGGATTCCCCGAGGATCGCAAGAACGCTTACGACCTCGCGACCATCCGCCACTGGCTCGAAAAGTTCCTCTGGCGC
TTCTTCCAGTTCAGCCAGTTCAAGCGCAGCGCGCTCCCCAATGGCCCCAAAGTCTCCAGCGGCGGTGCGCTCAGCCCGCG
CGGCGACTGGCGCGCGCCGAGCGATGCGGTGGCGGAGGTGTGGCTGGCCGAGCTAAAAGACAACGTACCGGAGAGCTGA

Upstream 100 bases:

>100_bases
CTCACCCGCCACGAACGCGGCCTGATCGAAATGCTGCGGCGCGGGCCCAAGGCACAGGAATTGCTTGCCGCGTAGTATCC
TCTTGGGATACCGATCCGCC

Downstream 100 bases:

>100_bases
CCCGCGATGAATGCCAAGAGCCTCGCAATCATGCTGTTCTGCAATTGCGCCTGGGCCTTCAACACCATCGTCAGCAAATG
GGCGGTCGACGATCTCGCCG

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 772; Mature: 772

Protein sequence:

>772_residues
MHQAGAKIRRFRETHDPPLSAEEFGARYGAPQPWASRTVYGWEVKGKVARPPVQKRLAALGVCDPGDWLEEARPEDNIPT
RKGTARMSDAAHPFYDRHRQGMIRVATATPAVRTADVPFNRDGIIAEARKAHDLEVDLVVYPELCVSSYAIDDLHLQTAM
LDASEAAVGAIAQATRDLSPVLIIGAPLRRNGKIYNCALALSRGRVLGVVPKSFLPNYREFYEKRHFAHGRNCQGLEIAL
NGERMPFGTDLIFAAHDLPGFTFGIEICEDFWSPNPPGTLAALASATILANLSASPITIGRADDRHLHCRSSSARSICAY
AYSASGFGESTTDLSWDGQGVVYEMGELMKQSTRFDLDTELCAVDIDIQRILAERMRNQTFSDAAEHEGRPEDWYRRIEF
QHAGTGGDTGLMRPIRRFPFVPNNRATLDEDCYEAFNIQVDALMRRIKATGPKSLVIGISGGLDSTHALIVAAKACDRLG
LPRTTIRGYTMPGFATSDYTKSNAWKLMQAMEITAEEIDIKPTARTMLENIGHAFADGEPVYDTTFENVQAGLRTDYLFR
LAGQHKGFVVGTGDLSELALGWCTYGVGDHMSHYGVNAGVPKTLIQYLIRWTIETEQFVPECSAVLGAILDTEISPELVP
AGEDGEIQSTESTIGPYELNDFFLHHIVRYGQSPSKVAFLAWHAWKDIEAGEWPEGFPEDRKNAYDLATIRHWLEKFLWR
FFQFSQFKRSALPNGPKVSSGGALSPRGDWRAPSDAVAEVWLAELKDNVPES

Sequences:

>Translated_772_residues
MHQAGAKIRRFRETHDPPLSAEEFGARYGAPQPWASRTVYGWEVKGKVARPPVQKRLAALGVCDPGDWLEEARPEDNIPT
RKGTARMSDAAHPFYDRHRQGMIRVATATPAVRTADVPFNRDGIIAEARKAHDLEVDLVVYPELCVSSYAIDDLHLQTAM
LDASEAAVGAIAQATRDLSPVLIIGAPLRRNGKIYNCALALSRGRVLGVVPKSFLPNYREFYEKRHFAHGRNCQGLEIAL
NGERMPFGTDLIFAAHDLPGFTFGIEICEDFWSPNPPGTLAALASATILANLSASPITIGRADDRHLHCRSSSARSICAY
AYSASGFGESTTDLSWDGQGVVYEMGELMKQSTRFDLDTELCAVDIDIQRILAERMRNQTFSDAAEHEGRPEDWYRRIEF
QHAGTGGDTGLMRPIRRFPFVPNNRATLDEDCYEAFNIQVDALMRRIKATGPKSLVIGISGGLDSTHALIVAAKACDRLG
LPRTTIRGYTMPGFATSDYTKSNAWKLMQAMEITAEEIDIKPTARTMLENIGHAFADGEPVYDTTFENVQAGLRTDYLFR
LAGQHKGFVVGTGDLSELALGWCTYGVGDHMSHYGVNAGVPKTLIQYLIRWTIETEQFVPECSAVLGAILDTEISPELVP
AGEDGEIQSTESTIGPYELNDFFLHHIVRYGQSPSKVAFLAWHAWKDIEAGEWPEGFPEDRKNAYDLATIRHWLEKFLWR
FFQFSQFKRSALPNGPKVSSGGALSPRGDWRAPSDAVAEVWLAELKDNVPES
>Mature_772_residues
MHQAGAKIRRFRETHDPPLSAEEFGARYGAPQPWASRTVYGWEVKGKVARPPVQKRLAALGVCDPGDWLEEARPEDNIPT
RKGTARMSDAAHPFYDRHRQGMIRVATATPAVRTADVPFNRDGIIAEARKAHDLEVDLVVYPELCVSSYAIDDLHLQTAM
LDASEAAVGAIAQATRDLSPVLIIGAPLRRNGKIYNCALALSRGRVLGVVPKSFLPNYREFYEKRHFAHGRNCQGLEIAL
NGERMPFGTDLIFAAHDLPGFTFGIEICEDFWSPNPPGTLAALASATILANLSASPITIGRADDRHLHCRSSSARSICAY
AYSASGFGESTTDLSWDGQGVVYEMGELMKQSTRFDLDTELCAVDIDIQRILAERMRNQTFSDAAEHEGRPEDWYRRIEF
QHAGTGGDTGLMRPIRRFPFVPNNRATLDEDCYEAFNIQVDALMRRIKATGPKSLVIGISGGLDSTHALIVAAKACDRLG
LPRTTIRGYTMPGFATSDYTKSNAWKLMQAMEITAEEIDIKPTARTMLENIGHAFADGEPVYDTTFENVQAGLRTDYLFR
LAGQHKGFVVGTGDLSELALGWCTYGVGDHMSHYGVNAGVPKTLIQYLIRWTIETEQFVPECSAVLGAILDTEISPELVP
AGEDGEIQSTESTIGPYELNDFFLHHIVRYGQSPSKVAFLAWHAWKDIEAGEWPEGFPEDRKNAYDLATIRHWLEKFLWR
FFQFSQFKRSALPNGPKVSSGGALSPRGDWRAPSDAVAEVWLAELKDNVPES

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17538756, Length=687, Percent_Identity=23.1441048034935, Blast_Score=84, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321865, Length=390, Percent_Identity=24.1025641025641, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24641841, Length=611, Percent_Identity=21.4402618657938, Blast_Score=73, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24641843, Length=611, Percent_Identity=21.4402618657938, Blast_Score=73, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 85543; Mature: 85543

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQAGAKIRRFRETHDPPLSAEEFGARYGAPQPWASRTVYGWEVKGKVARPPVQKRLAAL
CCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHH
GVCDPGDWLEEARPEDNIPTRKGTARMSDAAHPFYDRHRQGMIRVATATPAVRTADVPFN
CCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEEEECCCCEEECCCCCC
RDGIIAEARKAHDLEVDLVVYPELCVSSYAIDDLHLQTAMLDASEAAVGAIAQATRDLSP
CCCCHHHHHCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCC
VLIIGAPLRRNGKIYNCALALSRGRVLGVVPKSFLPNYREFYEKRHFAHGRNCQGLEIAL
EEEEECCCCCCCCEEEEEEEECCCCEEEECCHHHCCCHHHHHHHHHHCCCCCCCCEEEEE
NGERMPFGTDLIFAAHDLPGFTFGIEICEDFWSPNPPGTLAALASATILANLSASPITIG
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEECCCCCEEEE
RADDRHLHCRSSSARSICAYAYSASGFGESTTDLSWDGQGVVYEMGELMKQSTRFDLDTE
CCCCCEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
LCAVDIDIQRILAERMRNQTFSDAAEHEGRPEDWYRRIEFQHAGTGGDTGLMRPIRRFPF
EEEEEHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHEEEEECCCCCCHHHHHHHHHCCC
VPNNRATLDEDCYEAFNIQVDALMRRIKATGPKSLVIGISGGLDSTHALIVAAKACDRLG
CCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHEEEHHHHHHCC
LPRTTIRGYTMPGFATSDYTKSNAWKLMQAMEITAEEIDIKPTARTMLENIGHAFADGEP
CCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
VYDTTFENVQAGLRTDYLFRLAGQHKGFVVGTGDLSELALGWCTYGVGDHMSHYGVNAGV
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHCCCCCCC
PKTLIQYLIRWTIETEQFVPECSAVLGAILDTEISPELVPAGEDGEIQSTESTIGPYELN
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCHH
DFFLHHIVRYGQSPSKVAFLAWHAWKDIEAGEWPEGFPEDRKNAYDLATIRHWLEKFLWR
HHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHH
FFQFSQFKRSALPNGPKVSSGGALSPRGDWRAPSDAVAEVWLAELKDNVPES
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHQAGAKIRRFRETHDPPLSAEEFGARYGAPQPWASRTVYGWEVKGKVARPPVQKRLAAL
CCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHH
GVCDPGDWLEEARPEDNIPTRKGTARMSDAAHPFYDRHRQGMIRVATATPAVRTADVPFN
CCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEEEECCCCEEECCCCCC
RDGIIAEARKAHDLEVDLVVYPELCVSSYAIDDLHLQTAMLDASEAAVGAIAQATRDLSP
CCCCHHHHHCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCC
VLIIGAPLRRNGKIYNCALALSRGRVLGVVPKSFLPNYREFYEKRHFAHGRNCQGLEIAL
EEEEECCCCCCCCEEEEEEEECCCCEEEECCHHHCCCHHHHHHHHHHCCCCCCCCEEEEE
NGERMPFGTDLIFAAHDLPGFTFGIEICEDFWSPNPPGTLAALASATILANLSASPITIG
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEECCCCCEEEE
RADDRHLHCRSSSARSICAYAYSASGFGESTTDLSWDGQGVVYEMGELMKQSTRFDLDTE
CCCCCEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
LCAVDIDIQRILAERMRNQTFSDAAEHEGRPEDWYRRIEFQHAGTGGDTGLMRPIRRFPF
EEEEEHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHEEEEECCCCCCHHHHHHHHHCCC
VPNNRATLDEDCYEAFNIQVDALMRRIKATGPKSLVIGISGGLDSTHALIVAAKACDRLG
CCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHEEEHHHHHHCC
LPRTTIRGYTMPGFATSDYTKSNAWKLMQAMEITAEEIDIKPTARTMLENIGHAFADGEP
CCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
VYDTTFENVQAGLRTDYLFRLAGQHKGFVVGTGDLSELALGWCTYGVGDHMSHYGVNAGV
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHCCCCCCC
PKTLIQYLIRWTIETEQFVPECSAVLGAILDTEISPELVPAGEDGEIQSTESTIGPYELN
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCHH
DFFLHHIVRYGQSPSKVAFLAWHAWKDIEAGEWPEGFPEDRKNAYDLATIRHWLEKFLWR
HHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHH
FFQFSQFKRSALPNGPKVSSGGALSPRGDWRAPSDAVAEVWLAELKDNVPES
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]