Click here to switch to the map view.

The map label for this gene is ilvE [H]

Identifier: 85374501

GI number: 85374501

Start: 1713495

End: 1714574

Strand: Direct

Name: ilvE [H]

Synonym: ELI_08370

Alternate gene names: 85374501

Gene position: 1713495-1714574 (Clockwise)

Preceding gene: 85374500

Following gene: 85374502

Centisome position: 56.14

GC content: 64.44

Gene sequence:

>1080_bases
ATGCAATTCACAAGCGTTCCCCACCCTGCCCCGACGCACGAAAACGTCCGCAAGGCGGCGATTGCCGATCCCGGTTTCGG
CACGGTGTTCACCGATCACATGGTCACTGTCGACTATGACGAAGCGAAGGGCGGCTGGCACTCTGCACAGATCGGCCCGC
GCGAAGCCATCGCGCTCGATCCGGCGGCAAGCGTGCTGCACTATGCGCAGGAAATCTTCGAAGGGATGAAGGCGTACCAG
CACCCGGACGGCGGCCTGGCGCTTTTCCGGCCGGAGGAAAACGCGCGCCGCTTCAACGCCAGCGCGCGCCGCATGGCGAT
GCCGGAGATTCCGGAAAAGCTGTTCCTCGATGCGGTGAAGCTGGCGGTCGAGACCGATGCCGATTGGATGCCGCCGGTCG
AAGGCGGCACGCTTTATATCCGGCCTTTCATGTTTGCTTCGGAAGCATTCCTCGGCGTGCGGCCGGCCAAGCAGTACAAG
TTCGTCGTGATCCTCGTCTCCTCGGGCAATTACTTCAAGAACGGTGTGAACCCGGTGCATATCTGGGTCGCCCAGGATTA
TGTCCGCGCCGCCCCCGGCGGCACCGGCGCGGCCAAGACGGGCGGCAATTACGCCGCATCGCTCGTCCCCCAGGCCGAGG
CGATCGCACAGGGCTGCGACCAGGTCGTCTTCCTCGACGCGATCGAGCACAAATGGGTCGAGGAACTGGGCGGCATGAAC
CTGTTCTTCGTGCGGCAGGACGGCAGCGTCATTACCCCGCCGCTGACCGGTACGATCCTGCCCGGCATCACCCGCGACAG
CCTGATCGCTATGCTGCGCGAAGAGGGGCTGGAGGTGCGCGAGGAGCCCTATTCGATCCAGCAATGGCGCGAAGAGGCCG
AGAACGGCATGCTGCTGGAAACGCTCGCCTGTGGCACGGCAGCCGTGGTCACGCCGGTGGGCAAGGTGTCCTCCCCTGAT
GGTTCGTTCGAAATCGGCACCGGCGGCATCGGCCAAATGGCGCAGAAGATGCGCGAGCGGCTGGTCGGCATCCAGACCGG
CGAAGTAGCCGACACGCACGGCTGGGTAGTGAAGGTCTGA

Upstream 100 bases:

>100_bases
CCGGCGCTTGAGGCCCAACAAAAGACTCCTCGCCCAAATCCTCCCGCTCCCCAACCAACACCCTTGTCCAATCACCCGCC
GGACATTATCCGCGCACACC

Downstream 100 bases:

>100_bases
TCTTCATGTCGGGCCACCCGCCCATTACCGTTGCCGCGCTCTACCAGTTTACGCGCTTCGACGATCCCGCTTCGGTCCGC
GCACCGTTGCTCGCCGCATG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MQFTSVPHPAPTHENVRKAAIADPGFGTVFTDHMVTVDYDEAKGGWHSAQIGPREAIALDPAASVLHYAQEIFEGMKAYQ
HPDGGLALFRPEENARRFNASARRMAMPEIPEKLFLDAVKLAVETDADWMPPVEGGTLYIRPFMFASEAFLGVRPAKQYK
FVVILVSSGNYFKNGVNPVHIWVAQDYVRAAPGGTGAAKTGGNYAASLVPQAEAIAQGCDQVVFLDAIEHKWVEELGGMN
LFFVRQDGSVITPPLTGTILPGITRDSLIAMLREEGLEVREEPYSIQQWREEAENGMLLETLACGTAAVVTPVGKVSSPD
GSFEIGTGGIGQMAQKMRERLVGIQTGEVADTHGWVVKV

Sequences:

>Translated_359_residues
MQFTSVPHPAPTHENVRKAAIADPGFGTVFTDHMVTVDYDEAKGGWHSAQIGPREAIALDPAASVLHYAQEIFEGMKAYQ
HPDGGLALFRPEENARRFNASARRMAMPEIPEKLFLDAVKLAVETDADWMPPVEGGTLYIRPFMFASEAFLGVRPAKQYK
FVVILVSSGNYFKNGVNPVHIWVAQDYVRAAPGGTGAAKTGGNYAASLVPQAEAIAQGCDQVVFLDAIEHKWVEELGGMN
LFFVRQDGSVITPPLTGTILPGITRDSLIAMLREEGLEVREEPYSIQQWREEAENGMLLETLACGTAAVVTPVGKVSSPD
GSFEIGTGGIGQMAQKMRERLVGIQTGEVADTHGWVVKV
>Mature_359_residues
MQFTSVPHPAPTHENVRKAAIADPGFGTVFTDHMVTVDYDEAKGGWHSAQIGPREAIALDPAASVLHYAQEIFEGMKAYQ
HPDGGLALFRPEENARRFNASARRMAMPEIPEKLFLDAVKLAVETDADWMPPVEGGTLYIRPFMFASEAFLGVRPAKQYK
FVVILVSSGNYFKNGVNPVHIWVAQDYVRAAPGGTGAAKTGGNYAASLVPQAEAIAQGCDQVVFLDAIEHKWVEELGGMN
LFFVRQDGSVITPPLTGTILPGITRDSLIAMLREEGLEVREEPYSIQQWREEAENGMLLETLACGTAAVVTPVGKVSSPD
GSFEIGTGGIGQMAQKMRERLVGIQTGEVADTHGWVVKV

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI38176287, Length=329, Percent_Identity=37.9939209726444, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI296010906, Length=329, Percent_Identity=37.9939209726444, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI296010904, Length=329, Percent_Identity=37.9939209726444, Blast_Score=221, Evalue=6e-58,
Organism=Homo sapiens, GI50658084, Length=370, Percent_Identity=35.4054054054054, Blast_Score=214, Evalue=8e-56,
Organism=Homo sapiens, GI296010900, Length=329, Percent_Identity=33.7386018237082, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI296010902, Length=329, Percent_Identity=33.7386018237082, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI258614015, Length=296, Percent_Identity=35.8108108108108, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI48994963, Length=322, Percent_Identity=31.6770186335404, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17568601, Length=312, Percent_Identity=37.1794871794872, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17565728, Length=310, Percent_Identity=37.0967741935484, Blast_Score=188, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6322608, Length=338, Percent_Identity=37.2781065088757, Blast_Score=209, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6322002, Length=361, Percent_Identity=34.6260387811634, Blast_Score=204, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24641779, Length=386, Percent_Identity=37.5647668393782, Blast_Score=236, Evalue=1e-62,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 38954; Mature: 38954

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFTSVPHPAPTHENVRKAAIADPGFGTVFTDHMVTVDYDEAKGGWHSAQIGPREAIALD
CCCCCCCCCCCCHHHHHHHHCCCCCCCCEEECCEEEEECCCCCCCCCCCCCCCCCEEEEC
PAASVLHYAQEIFEGMKAYQHPDGGLALFRPEENARRFNASARRMAMPEIPEKLFLDAVK
HHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHCCHHHHCCCCCCCHHHHHHHHH
LAVETDADWMPPVEGGTLYIRPFMFASEAFLGVRPAKQYKFVVILVSSGNYFKNGVNPVH
HHEECCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCCEEEEEEEEECCCHHHCCCCEEE
IWVAQDYVRAAPGGTGAAKTGGNYAASLVPQAEAIAQGCDQVVFLDAIEHKWVEELGGMN
EEEEHHHHHCCCCCCCCCCCCCCEEEHHCCHHHHHHCCCCCEEEHHHHHHHHHHHHCCCE
LFFVRQDGSVITPPLTGTILPGITRDSLIAMLREEGLEVREEPYSIQQWREEAENGMLLE
EEEEECCCCEECCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHCCEEEE
TLACGTAAVVTPVGKVSSPDGSFEIGTGGIGQMAQKMRERLVGIQTGEVADTHGWVVKV
EHHCCCEEEECCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEC
>Mature Secondary Structure
MQFTSVPHPAPTHENVRKAAIADPGFGTVFTDHMVTVDYDEAKGGWHSAQIGPREAIALD
CCCCCCCCCCCCHHHHHHHHCCCCCCCCEEECCEEEEECCCCCCCCCCCCCCCCCEEEEC
PAASVLHYAQEIFEGMKAYQHPDGGLALFRPEENARRFNASARRMAMPEIPEKLFLDAVK
HHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHCCHHHHCCCCCCCHHHHHHHHH
LAVETDADWMPPVEGGTLYIRPFMFASEAFLGVRPAKQYKFVVILVSSGNYFKNGVNPVH
HHEECCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCCEEEEEEEEECCCHHHCCCCEEE
IWVAQDYVRAAPGGTGAAKTGGNYAASLVPQAEAIAQGCDQVVFLDAIEHKWVEELGGMN
EEEEHHHHHCCCCCCCCCCCCCCEEEHHCCHHHHHHCCCCCEEEHHHHHHHHHHHHCCCE
LFFVRQDGSVITPPLTGTILPGITRDSLIAMLREEGLEVREEPYSIQQWREEAENGMLLE
EEEEECCCCEECCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHCCEEEE
TLACGTAAVVTPVGKVSSPDGSFEIGTGGIGQMAQKMRERLVGIQTGEVADTHGWVVKV
EHHCCCEEEECCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]