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The map label for this gene is leuB [H]

Identifier: 85374494

GI number: 85374494

Start: 1708652

End: 1709710

Strand: Reverse

Name: leuB [H]

Synonym: ELI_08335

Alternate gene names: 85374494

Gene position: 1709710-1708652 (Counterclockwise)

Preceding gene: 85374495

Following gene: 85374493

Centisome position: 56.01

GC content: 65.91

Gene sequence:

>1059_bases
ATGAAAATAGCGGTTTTTCCCGGCGATGGCATCGGCCCCGAAGTGACCCGCGAGGCGGTGCGCGTGCTGGAGGCGCTGGG
CCTGCCTGGGCTGGTGCTGTTCGAAGGCGATGTCGGTGCCGCCGCCTACCGCAAGCACGGCCAACCGCTGCCCGACGAGA
CGCTCAACATGGCGCGCGAGGCGGATGCGGTGCTGTTCGGCGCAGTCGGCGATTCTTCGTGCGATGGGCTGGAGCGTGCG
CTCCGCCCCGAACAGGCGGTGCTGGGCTTGCGGGCCGAACTCGGCCTCTTTGCAAACCTTCGCCCGGCGACGCTCTTTCC
GGGGCTGGAAGAGCATTCCGCGCTCCGCCCTGAAGTCGCCCGCCAGATCGACCTGCTGATCGTGCGCGAGCTCAATGGCG
ATGTCTATTTCGGCGACAAGGGCCAGCGCCGGACCGAAGACGGGCGGCGCGAAGGCTGGGATATAATGTCCTATGCCGAA
GACGAGGTCCGCCGGATCGCCCACACCGCATTCCGCGCGGCGCAAGGGCGCGGCAAGAAACTGACCAGCGTCGACAAGGC
CAATGTGCTCGAGACGAGCCAGCTGTGGCGCGACGTGATGATCGAAGTGGCGACCGACTATCCCGACGTCGCGCTCGATC
ATCTTTATGTCGACAATGCCGCCATGCAATTGGTGCGCGCGCCGGGCCAATTCGACGTCATCGTCACCGGCAATTTGTTC
GGCGACATCCTGTCGGACCAGGCCAGCATGTGCGTCGGCTCGATCGGCCTGCTGCCCAGCGCTTCGCTGGGTGAGCGGCA
AACCGCGCACGGCACTTTTGGCCTCTACGAGCCGATCCACGGTAGCGCGCCCGATATTGCCGGGCAGGGCAAGGCCAACC
CACTCGCCGCGATCCTCTCGGCGGCGATGATGCTGCGGCACAGCTTCGGCAGGGAAGCCGAGGCCGAGCGGATCGAAGCG
GCAGTCGGCGCGACAGTGCGCGACGGCATTTTCGGCGCGGATCTCGGTGGCAGCGCTTCGACCGAGGAAATAGCTGACGC
GGTGCTGGAACAGCTTTAA

Upstream 100 bases:

>100_bases
GCGCTATTTGCTAGCCGACCGGCGCGGCGACACGCTGGCGGCGCGCGAGCAATTGCTGGCACGGCTGCGCAAGATGGTCT
GAAGGAAGGGCGGCAGTTCC

Downstream 100 bases:

>100_bases
GGCGCCGGGCCGATGCTCGACCTCGCCATCATCCTGCCCACGCTCGACGAACGCGAGAATATCGCGCCGCTGGTCGATCG
GCTCGACGCCGTCTTGGGCG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MKIAVFPGDGIGPEVTREAVRVLEALGLPGLVLFEGDVGAAAYRKHGQPLPDETLNMAREADAVLFGAVGDSSCDGLERA
LRPEQAVLGLRAELGLFANLRPATLFPGLEEHSALRPEVARQIDLLIVRELNGDVYFGDKGQRRTEDGRREGWDIMSYAE
DEVRRIAHTAFRAAQGRGKKLTSVDKANVLETSQLWRDVMIEVATDYPDVALDHLYVDNAAMQLVRAPGQFDVIVTGNLF
GDILSDQASMCVGSIGLLPSASLGERQTAHGTFGLYEPIHGSAPDIAGQGKANPLAAILSAAMMLRHSFGREAEAERIEA
AVGATVRDGIFGADLGGSASTEEIADAVLEQL

Sequences:

>Translated_352_residues
MKIAVFPGDGIGPEVTREAVRVLEALGLPGLVLFEGDVGAAAYRKHGQPLPDETLNMAREADAVLFGAVGDSSCDGLERA
LRPEQAVLGLRAELGLFANLRPATLFPGLEEHSALRPEVARQIDLLIVRELNGDVYFGDKGQRRTEDGRREGWDIMSYAE
DEVRRIAHTAFRAAQGRGKKLTSVDKANVLETSQLWRDVMIEVATDYPDVALDHLYVDNAAMQLVRAPGQFDVIVTGNLF
GDILSDQASMCVGSIGLLPSASLGERQTAHGTFGLYEPIHGSAPDIAGQGKANPLAAILSAAMMLRHSFGREAEAERIEA
AVGATVRDGIFGADLGGSASTEEIADAVLEQL
>Mature_352_residues
MKIAVFPGDGIGPEVTREAVRVLEALGLPGLVLFEGDVGAAAYRKHGQPLPDETLNMAREADAVLFGAVGDSSCDGLERA
LRPEQAVLGLRAELGLFANLRPATLFPGLEEHSALRPEVARQIDLLIVRELNGDVYFGDKGQRRTEDGRREGWDIMSYAE
DEVRRIAHTAFRAAQGRGKKLTSVDKANVLETSQLWRDVMIEVATDYPDVALDHLYVDNAAMQLVRAPGQFDVIVTGNLF
GDILSDQASMCVGSIGLLPSASLGERQTAHGTFGLYEPIHGSAPDIAGQGKANPLAAILSAAMMLRHSFGREAEAERIEA
AVGATVRDGIFGADLGGSASTEEIADAVLEQL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=353, Percent_Identity=31.728045325779, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI4758582, Length=359, Percent_Identity=29.8050139275766, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI28178816, Length=354, Percent_Identity=29.0960451977401, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI28178821, Length=354, Percent_Identity=28.8135593220339, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI28178838, Length=332, Percent_Identity=30.421686746988, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI28178819, Length=208, Percent_Identity=33.1730769230769, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI87081683, Length=354, Percent_Identity=48.0225988700565, Blast_Score=308, Evalue=4e-85,
Organism=Escherichia coli, GI1788101, Length=361, Percent_Identity=37.3961218836565, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1787381, Length=404, Percent_Identity=26.980198019802, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71986051, Length=354, Percent_Identity=32.7683615819209, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17550882, Length=356, Percent_Identity=31.1797752808989, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI25144293, Length=357, Percent_Identity=31.0924369747899, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17505779, Length=362, Percent_Identity=29.0055248618785, Blast_Score=121, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6319830, Length=360, Percent_Identity=44.7222222222222, Blast_Score=272, Evalue=5e-74,
Organism=Saccharomyces cerevisiae, GI6322097, Length=368, Percent_Identity=39.4021739130435, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6324709, Length=357, Percent_Identity=31.0924369747899, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6324291, Length=357, Percent_Identity=30.812324929972, Blast_Score=133, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24643270, Length=355, Percent_Identity=32.3943661971831, Blast_Score=161, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24643268, Length=355, Percent_Identity=32.3943661971831, Blast_Score=160, Evalue=9e-40,
Organism=Drosophila melanogaster, GI24661184, Length=359, Percent_Identity=30.6406685236769, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI161078633, Length=357, Percent_Identity=28.5714285714286, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24650122, Length=357, Percent_Identity=28.5714285714286, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI161078635, Length=357, Percent_Identity=28.5714285714286, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI161078637, Length=357, Percent_Identity=28.5714285714286, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI161078639, Length=355, Percent_Identity=28.7323943661972, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI281362242, Length=351, Percent_Identity=28.2051282051282, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24648872, Length=351, Percent_Identity=28.2051282051282, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI20130355, Length=192, Percent_Identity=27.6041666666667, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 37613; Mature: 37613

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVFPGDGIGPEVTREAVRVLEALGLPGLVLFEGDVGAAAYRKHGQPLPDETLNMARE
CEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCCHHHHHHHHH
ADAVLFGAVGDSSCDGLERALRPEQAVLGLRAELGLFANLRPATLFPGLEEHSALRPEVA
CCEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHCCCHHHH
RQIDLLIVRELNGDVYFGDKGQRRTEDGRREGWDIMSYAEDEVRRIAHTAFRAAQGRGKK
HHHHEEEEEECCCCEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
LTSVDKANVLETSQLWRDVMIEVATDYPDVALDHLYVDNAAMQLVRAPGQFDVIVTGNLF
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHH
GDILSDQASMCVGSIGLLPSASLGERQTAHGTFGLYEPIHGSAPDIAGQGKANPLAAILS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
AAMMLRHSFGREAEAERIEAAVGATVRDGIFGADLGGSASTEEIADAVLEQL
HHHHHHHHCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MKIAVFPGDGIGPEVTREAVRVLEALGLPGLVLFEGDVGAAAYRKHGQPLPDETLNMARE
CEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCCHHHHHHHHH
ADAVLFGAVGDSSCDGLERALRPEQAVLGLRAELGLFANLRPATLFPGLEEHSALRPEVA
CCEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHCCCHHHH
RQIDLLIVRELNGDVYFGDKGQRRTEDGRREGWDIMSYAEDEVRRIAHTAFRAAQGRGKK
HHHHEEEEEECCCCEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
LTSVDKANVLETSQLWRDVMIEVATDYPDVALDHLYVDNAAMQLVRAPGQFDVIVTGNLF
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHH
GDILSDQASMCVGSIGLLPSASLGERQTAHGTFGLYEPIHGSAPDIAGQGKANPLAAILS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
AAMMLRHSFGREAEAERIEAAVGATVRDGIFGADLGGSASTEEIADAVLEQL
HHHHHHHHCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA