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The map label for this gene is rpoA

Identifier: 85374439

GI number: 85374439

Start: 1670077

End: 1671132

Strand: Reverse

Name: rpoA

Synonym: ELI_08060

Alternate gene names: 85374439

Gene position: 1671132-1670077 (Counterclockwise)

Preceding gene: 85374440

Following gene: 85374438

Centisome position: 54.75

GC content: 61.17

Gene sequence:

>1056_bases
ATGTCCGTGAACACCAAGAACTGGCAGGAACTCAAGAAACCCACGCAGCTCGACGTCAAGGAGAGCAGCGACAAGGCCCG
CAAGGCGACCTTCATCGCCGAGCCGCTCGAGCGTGGCTACGGCCTTACGCTCGGCAACGCACTGCGCCGGGTCCTCCTGG
CCTCGCTCCAGGGCGCTGCCATCACCTCGATCAAGATCGAGAACGTGCTGCACGAATTCAGCTCGCTTGCCGGCGTGCGT
GAAGACGTGACCGACATCGTCCTCAATGTGAAGCAGATCGCTCTGAAGATGGAAGGCGAAGGCCCCAAGCGCCTTCAGCT
TTCCGCCACCGGCCCGGCCGAAGTGAAGGCCGGCGACATCGCGGTTTCGGGCGATATCGAAGTGATGAACAAGGATCTCG
TGATCTGTCATCTCGACGAAGGCGCGACGCTCAACATGGAACTGACTGCCGATATCGGCAGCGGCTATGTCCCGGCGGTC
CAGAACCGTCCGGCCGATGCGCCGATCGGCCTGATCCCGGTCGACAGCCTCTATTCGCCGATCCGTCAGGTCAGCTACAA
GGTCGAAAAGGCCCGTGTCGGCCAGGAGCTCGACTTCGACAAGCTGAGCCTGACCATCGAAACCGACGGCACCGTCACCC
CTGAAGATGCCGTGGCCTATGCGGCGCGCATCCTGCAGGACCAGTTGACGCTGTTCGTCCACTTCGAAGACGGTATCCCG
CAGCCGCAGAGCGCCATGATCGGCGTCGCTGCCGAGCCGCAGCAGGACGATGCCAACCAGCTCAATCGTTACCTCCTCAA
GAAGGTCGACGAGCTGGAACTGTCGGTCCGCTCGGCCAACTGCCTCAAGAACGACAACATCATCTATATCGGCGACCTGG
TCCAGAAGACCGAAGCCGAGATGCTGCGCACGCCGAATTTCGGTCGCAAGTCGCTCAACGAGATCAAGGAAGTTCTCTCC
AGCATGGGTCTGCGCCTCGGCATGGACATCCCCGGCTGGCCGCCCGAGAACATCGAGGAAATGGCCAAGAAGCTGGAGCA
GGAATTACTCGGGTAA

Upstream 100 bases:

>100_bases
GTGCCTGCCCGGCGGCCATTCGGTCGCCGACACCTCTTACGGACCGGACGCGCCCACAAGCGCTCCGGTCCCGCCCGCAT
CCAAGTCCCAGGGGAATTTC

Downstream 100 bases:

>100_bases
TTCCTGCGGAAGGCCCCGCGCAGGCGGGGGCCTTTCGCCGCTGGCGCTTCGCCGGTGGCCTGAGACCCCCGCCTGCGCGG
GGGATCGCAGCAGGGCGAAG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MSVNTKNWQELKKPTQLDVKESSDKARKATFIAEPLERGYGLTLGNALRRVLLASLQGAAITSIKIENVLHEFSSLAGVR
EDVTDIVLNVKQIALKMEGEGPKRLQLSATGPAEVKAGDIAVSGDIEVMNKDLVICHLDEGATLNMELTADIGSGYVPAV
QNRPADAPIGLIPVDSLYSPIRQVSYKVEKARVGQELDFDKLSLTIETDGTVTPEDAVAYAARILQDQLTLFVHFEDGIP
QPQSAMIGVAAEPQQDDANQLNRYLLKKVDELELSVRSANCLKNDNIIYIGDLVQKTEAEMLRTPNFGRKSLNEIKEVLS
SMGLRLGMDIPGWPPENIEEMAKKLEQELLG

Sequences:

>Translated_351_residues
MSVNTKNWQELKKPTQLDVKESSDKARKATFIAEPLERGYGLTLGNALRRVLLASLQGAAITSIKIENVLHEFSSLAGVR
EDVTDIVLNVKQIALKMEGEGPKRLQLSATGPAEVKAGDIAVSGDIEVMNKDLVICHLDEGATLNMELTADIGSGYVPAV
QNRPADAPIGLIPVDSLYSPIRQVSYKVEKARVGQELDFDKLSLTIETDGTVTPEDAVAYAARILQDQLTLFVHFEDGIP
QPQSAMIGVAAEPQQDDANQLNRYLLKKVDELELSVRSANCLKNDNIIYIGDLVQKTEAEMLRTPNFGRKSLNEIKEVLS
SMGLRLGMDIPGWPPENIEEMAKKLEQELLG
>Mature_350_residues
SVNTKNWQELKKPTQLDVKESSDKARKATFIAEPLERGYGLTLGNALRRVLLASLQGAAITSIKIENVLHEFSSLAGVRE
DVTDIVLNVKQIALKMEGEGPKRLQLSATGPAEVKAGDIAVSGDIEVMNKDLVICHLDEGATLNMELTADIGSGYVPAVQ
NRPADAPIGLIPVDSLYSPIRQVSYKVEKARVGQELDFDKLSLTIETDGTVTPEDAVAYAARILQDQLTLFVHFEDGIPQ
PQSAMIGVAAEPQQDDANQLNRYLLKKVDELELSVRSANCLKNDNIIYIGDLVQKTEAEMLRTPNFGRKSLNEIKEVLSS
MGLRLGMDIPGWPPENIEEMAKKLEQELLG

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=333, Percent_Identity=46.5465465465465, Blast_Score=296, Evalue=9e-82,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_ERYLH (Q2N9D7)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458501.1
- HSSP:   P20429
- ProteinModelPortal:   Q2N9D7
- STRING:   Q2N9D7
- GeneID:   3870560
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_08060
- NMPDR:   fig|314225.3.peg.1430
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- PhylomeDB:   Q2N9D7
- ProtClustDB:   PRK05182
- BioCyc:   ELIT314225:ELI_08060-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 38475; Mature: 38343

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVNTKNWQELKKPTQLDVKESSDKARKATFIAEPLERGYGLTLGNALRRVLLASLQGAA
CCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCE
ITSIKIENVLHEFSSLAGVREDVTDIVLNVKQIALKMEGEGPKRLQLSATGPAEVKAGDI
EEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEECCCCCCEEEEECCCCCCEECCCE
AVSGDIEVMNKDLVICHLDEGATLNMELTADIGSGYVPAVQNRPADAPIGLIPVDSLYSP
EECCCCEEECCCEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHH
IRQVSYKVEKARVGQELDFDKLSLTIETDGTVTPEDAVAYAARILQDQLTLFVHFEDGIP
HHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCEEEEEEECCCCC
QPQSAMIGVAAEPQQDDANQLNRYLLKKVDELELSVRSANCLKNDNIIYIGDLVQKTEAE
CCCHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHH
MLRTPNFGRKSLNEIKEVLSSMGLRLGMDIPGWPPENIEEMAKKLEQELLG
HHCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVNTKNWQELKKPTQLDVKESSDKARKATFIAEPLERGYGLTLGNALRRVLLASLQGAA
CCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCE
ITSIKIENVLHEFSSLAGVREDVTDIVLNVKQIALKMEGEGPKRLQLSATGPAEVKAGDI
EEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEECCCCCCEEEEECCCCCCEECCCE
AVSGDIEVMNKDLVICHLDEGATLNMELTADIGSGYVPAVQNRPADAPIGLIPVDSLYSP
EECCCCEEECCCEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHH
IRQVSYKVEKARVGQELDFDKLSLTIETDGTVTPEDAVAYAARILQDQLTLFVHFEDGIP
HHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCEEEEEEECCCCC
QPQSAMIGVAAEPQQDDANQLNRYLLKKVDELELSVRSANCLKNDNIIYIGDLVQKTEAE
CCCHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHH
MLRTPNFGRKSLNEIKEVLSSMGLRLGMDIPGWPPENIEEMAKKLEQELLG
HHCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA