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The map label for this gene is dapA

Identifier: 85374379

GI number: 85374379

Start: 1607615

End: 1608496

Strand: Reverse

Name: dapA

Synonym: ELI_07760

Alternate gene names: 85374379

Gene position: 1608496-1607615 (Counterclockwise)

Preceding gene: 85374383

Following gene: 85374378

Centisome position: 52.7

GC content: 63.15

Gene sequence:

>882_bases
ATGTTTTCGGGCTCTATTCCCGCCCTTGCGACTCCTTTTCGCGATGGAGCGTTCGACGAGGCCACGTTCCGGCGGCTGGT
CGACTGGCAGATTGAAAGCGGCTCCAGCGCGTTGGTGCCCTGCGGGACGACGGGCGAGGCTTCGACGCTTTCCAATGCCG
AGCATCACCGCGTTATCGAGGTCTGCATCGAACAGGCGGCGGGCCGCGTGCCGGTGATCGCGGGGTGCGGCAGCAACGAC
ACGCGCAACGCACTGCTGCACATGAATTTCTCCAAGAAAGCGGGCGCGGCTGCGGGGCTGTGCGTCGCGCCTTACTACAA
CCGTCCGAGCCAGGCTGGCCTGATCGCGCATTTCAGTTTCCTCGCCGAAAACAGCGACCTGCCGATCGTTCTTTACAACG
TGCCGAGCCGCACGGTGACCGATATAGAAGACGAAACCGTCGTCGAACTGGTGACCAAATATCCGGACCGGATCGTCGCC
ATAAAGGATGCCAGCGGCGACCTATCGCGCGTCGCCGACCACCGCATGGGGATCGGGCGCGACTTCTGCCAGCTGTCGGG
CAATGACGAATTGTGGCTGCCGCATTCGGCGGCCGGCGGTTCGGGCGCAATATCGGTCACGGCCAATGTCGCCCCGGCCT
TGTGCGCTGAGTTTCATTCCGCGATTGCCGCCAACGAACTGGCGAGAGCGCGCGAACTTAACGACCGCCTGTTCCCGCTC
CACTACGCGATGTTCAGCGACGCTTCGCCCGCGCCGGTCAAATATGCGCTCAGCCGGGTGATGGAAGAATTCACCGACGA
AGTCCGCCTGCCGATCGTTTCGGCCAGCGAGGCGTCGCGCAAGGCGGTGGACGAAGCGCTGGAGCATGCCGGGTTGATTT
AA

Upstream 100 bases:

>100_bases
CTGGCCAAGCTCTCCTTATCAGGCACTGAATGAAATCCTCACCATCATAACGGTCGAGGATAATACTATATCCCAGATTG
CTCAGGAAGGACCGCTTTTA

Downstream 100 bases:

>100_bases
TCGTGGGGCGACAATGACGAATATCGTCAAAGAGTATTGGTTCTACTGGCTCCTGCCGCTGTGGTTGCTCGCCGCCTGGC
AGGTTTCCGCCGGCACTCCG

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MFSGSIPALATPFRDGAFDEATFRRLVDWQIESGSSALVPCGTTGEASTLSNAEHHRVIEVCIEQAAGRVPVIAGCGSND
TRNALLHMNFSKKAGAAAGLCVAPYYNRPSQAGLIAHFSFLAENSDLPIVLYNVPSRTVTDIEDETVVELVTKYPDRIVA
IKDASGDLSRVADHRMGIGRDFCQLSGNDELWLPHSAAGGSGAISVTANVAPALCAEFHSAIAANELARARELNDRLFPL
HYAMFSDASPAPVKYALSRVMEEFTDEVRLPIVSASEASRKAVDEALEHAGLI

Sequences:

>Translated_293_residues
MFSGSIPALATPFRDGAFDEATFRRLVDWQIESGSSALVPCGTTGEASTLSNAEHHRVIEVCIEQAAGRVPVIAGCGSND
TRNALLHMNFSKKAGAAAGLCVAPYYNRPSQAGLIAHFSFLAENSDLPIVLYNVPSRTVTDIEDETVVELVTKYPDRIVA
IKDASGDLSRVADHRMGIGRDFCQLSGNDELWLPHSAAGGSGAISVTANVAPALCAEFHSAIAANELARARELNDRLFPL
HYAMFSDASPAPVKYALSRVMEEFTDEVRLPIVSASEASRKAVDEALEHAGLI
>Mature_293_residues
MFSGSIPALATPFRDGAFDEATFRRLVDWQIESGSSALVPCGTTGEASTLSNAEHHRVIEVCIEQAAGRVPVIAGCGSND
TRNALLHMNFSKKAGAAAGLCVAPYYNRPSQAGLIAHFSFLAENSDLPIVLYNVPSRTVTDIEDETVVELVTKYPDRIVA
IKDASGDLSRVADHRMGIGRDFCQLSGNDELWLPHSAAGGSGAISVTANVAPALCAEFHSAIAANELARARELNDRLFPL
HYAMFSDASPAPVKYALSRVMEEFTDEVRLPIVSASEASRKAVDEALEHAGLI

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=245, Percent_Identity=29.3877551020408, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1788823, Length=294, Percent_Identity=43.5374149659864, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI87082415, Length=298, Percent_Identity=31.2080536912752, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1786463, Length=244, Percent_Identity=29.9180327868852, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789620, Length=235, Percent_Identity=30.2127659574468, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_ERYLH (Q2N9J7)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458441.1
- HSSP:   Q9X1K9
- ProteinModelPortal:   Q2N9J7
- SMR:   Q2N9J7
- STRING:   Q2N9J7
- GeneID:   3871217
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_07760
- NMPDR:   fig|314225.3.peg.1490
- eggNOG:   COG0329
- HOGENOM:   HBG358848
- OMA:   ELLCYPM
- PhylomeDB:   Q2N9J7
- ProtClustDB:   PRK03170
- BioCyc:   ELIT314225:ELI_07760-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 31313; Mature: 31313

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 162-162 BINDING 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSGSIPALATPFRDGAFDEATFRRLVDWQIESGSSALVPCGTTGEASTLSNAEHHRVIE
CCCCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCCEEEECCCCCCCHHCCCCHHHHHHH
VCIEQAAGRVPVIAGCGSNDTRNALLHMNFSKKAGAAAGLCVAPYYNRPSQAGLIAHFSF
HHHHHHCCCCCEEEECCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCCCCCHHHHHHHH
LAENSDLPIVLYNVPSRTVTDIEDETVVELVTKYPDRIVAIKDASGDLSRVADHRMGIGR
HHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCC
DFCQLSGNDELWLPHSAAGGSGAISVTANVAPALCAEFHSAIAANELARARELNDRLFPL
HHHHCCCCCCEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHH
HYAMFSDASPAPVKYALSRVMEEFTDEVRLPIVSASEASRKAVDEALEHAGLI
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFSGSIPALATPFRDGAFDEATFRRLVDWQIESGSSALVPCGTTGEASTLSNAEHHRVIE
CCCCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCCEEEECCCCCCCHHCCCCHHHHHHH
VCIEQAAGRVPVIAGCGSNDTRNALLHMNFSKKAGAAAGLCVAPYYNRPSQAGLIAHFSF
HHHHHHCCCCCEEEECCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCCCCCHHHHHHHH
LAENSDLPIVLYNVPSRTVTDIEDETVVELVTKYPDRIVAIKDASGDLSRVADHRMGIGR
HHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCC
DFCQLSGNDELWLPHSAAGGSGAISVTANVAPALCAEFHSAIAANELARARELNDRLFPL
HHHHCCCCCCEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHH
HYAMFSDASPAPVKYALSRVMEEFTDEVRLPIVSASEASRKAVDEALEHAGLI
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA