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The map label for this gene is smpB

Identifier: 85374376

GI number: 85374376

Start: 1605424

End: 1605906

Strand: Reverse

Name: smpB

Synonym: ELI_07745

Alternate gene names: 85374376

Gene position: 1605906-1605424 (Counterclockwise)

Preceding gene: 85374378

Following gene: 85374375

Centisome position: 52.61

GC content: 63.15

Gene sequence:

>483_bases
ATGGCACGCCCCAAACCTGAAACCTTCGATAAGCAGAAGACCGTCGCCGAGAACCGGCGCGCACGGTTCGATTACGCGGT
CGAGGACAAGTTCGAAGCCGGGATCGCGTTGCAGGGAACCGAAGTGAAGGCCCTGCGCGCCGGCGAGGCCTCGATCGCGG
AAAGCTATGCCGAAGTGAGGGATGGCGAAGCGTGGCTGATCAATGCCAACATCCCGGAATACAGCCACGGCAATCGCTTG
AACCACCAGCCGCGCCGTCCGCGCAAGCTGCTGCTGCACAATCGCGAGATCGAGCGGTTGCTCGGTGCGGTCGAGCGAAA
GGGCATGACGCTGGTGCCGCTGAGCATATATTTCAATTCCCGCGGCCGAGCGAAGGTCGAGCTCGCGCTCGCCAAGGGCC
GGCAGGCGCAGGACAAGCGCGAGTACATCAAGGATCGCGACTGGAAGCGCGACAAGGCGCGGATCATGCGCGACAAGGGC
TGA

Upstream 100 bases:

>100_bases
TCTAGCGCGCAAGTCCCCGTCGCTGCAAATGCGTGTTTCCGAAATCCGCGCATAGCGGCCCTTTTCCTGATCGGGCGAAG
CGCCTACATGCCAGCGCCTT

Downstream 100 bases:

>100_bases
TGGGCCGCCGGCAAGAATAGCGCCGTATTTCGACCCACGATTCACCCTCTGGCCATTTTGCCCCTGCTACCCATATCGCT
AACCATGATCGCTTCGTCGG

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MARPKPETFDKQKTVAENRRARFDYAVEDKFEAGIALQGTEVKALRAGEASIAESYAEVRDGEAWLINANIPEYSHGNRL
NHQPRRPRKLLLHNREIERLLGAVERKGMTLVPLSIYFNSRGRAKVELALAKGRQAQDKREYIKDRDWKRDKARIMRDKG

Sequences:

>Translated_160_residues
MARPKPETFDKQKTVAENRRARFDYAVEDKFEAGIALQGTEVKALRAGEASIAESYAEVRDGEAWLINANIPEYSHGNRL
NHQPRRPRKLLLHNREIERLLGAVERKGMTLVPLSIYFNSRGRAKVELALAKGRQAQDKREYIKDRDWKRDKARIMRDKG
>Mature_159_residues
ARPKPETFDKQKTVAENRRARFDYAVEDKFEAGIALQGTEVKALRAGEASIAESYAEVRDGEAWLINANIPEYSHGNRLN
HQPRRPRKLLLHNREIERLLGAVERKGMTLVPLSIYFNSRGRAKVELALAKGRQAQDKREYIKDRDWKRDKARIMRDKG

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=146, Percent_Identity=50.6849315068493, Blast_Score=143, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_ERYLH (Q2N9K0)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458438.1
- ProteinModelPortal:   Q2N9K0
- SMR:   Q2N9K0
- STRING:   Q2N9K0
- GeneID:   3871214
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_07745
- NMPDR:   fig|314225.3.peg.1493
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- PhylomeDB:   Q2N9K0
- ProtClustDB:   PRK05422
- BioCyc:   ELIT314225:ELI_07745-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18500; Mature: 18369

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPKPETFDKQKTVAENRRARFDYAVEDKFEAGIALQGTEVKALRAGEASIAESYAEVR
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCEEEEECCCHHHHHHHHHHC
DGEAWLINANIPEYSHGNRLNHQPRRPRKLLLHNREIERLLGAVERKGMTLVPLSIYFNS
CCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCEEEEEEEEECC
RGRAKVELALAKGRQAQDKREYIKDRDWKRDKARIMRDKG
CCCEEEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
ARPKPETFDKQKTVAENRRARFDYAVEDKFEAGIALQGTEVKALRAGEASIAESYAEVR
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCEEEEECCCHHHHHHHHHHC
DGEAWLINANIPEYSHGNRLNHQPRRPRKLLLHNREIERLLGAVERKGMTLVPLSIYFNS
CCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCEEEEEEEEECC
RGRAKVELALAKGRQAQDKREYIKDRDWKRDKARIMRDKG
CCCEEEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA