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The map label for this gene is recA

Identifier: 85374373

GI number: 85374373

Start: 1601026

End: 1602084

Strand: Reverse

Name: recA

Synonym: ELI_07730

Alternate gene names: 85374373

Gene position: 1602084-1601026 (Counterclockwise)

Preceding gene: 85374374

Following gene: 85374372

Centisome position: 52.49

GC content: 61.0

Gene sequence:

>1059_bases
ATGTCCGCCAATCTGAAGCTGGTTGAAAAGGAAAACAACGTGGACCGTCAGAAGGCGCTTGACGCAGCACTCGCCCAGAT
CGACCGCGCGTTCGGCAAGGGCTCGGCGATGAAGCTGGGTTCGAAGGAAGCGATGAATGTCGAAGCGATTTCGACCGGGT
CGCTCGGGCTGGATATCGCGCTTGGCATCGGCGGCCTCCCCAAGGGCCGCGTGATCGAAGTCTATGGCCCGGAAAGCTCG
GGCAAGACAACGCTCGCGCTGCACGCCATCGCCGAAGCCCAGAAGGCCGGCGGGACCGCTGCCTTCGTCGATGCCGAGCA
TGCGCTCGACCCTGTCTATGCCAAGGCACTGGGCGTCGACATCGATGAATTGATCGTCTCGCAGCCCGATACCGGCGAGC
AGGCGCTCGAGATTACCGACACATTGGTGCGTTCCAATGCAGTCGATATCCTCGTCGTCGATTCGGTCGCCGCTCTGGTT
CCGCGTGCGGAGATCGAAGGCGAGATGGGCGACAGCCACGTCGGCCTGCAGGCCCGCCTGATGAGCCAGTCGCTACGCAA
GCTGACCGGCTCGATCAACCGTTCCAAGTGCATGGTGATCTTCATCAACCAGCTGCGCATGAAGATCGGTGTGATGTACG
GCAACCCCGAGACGACGACGGGCGGAAACGCGCTCAAGTTCTACGCTTCGGTCCGGCTGGACATTCGCCGTACCGGCCAG
ATCAAGGACCGGGACGAAGTGGTCGGCAACTCGACCCGCGTGAAGGTTGTCAAGAACAAGGTCGCCCCGCCGTTCAAGCA
GGTCGAATTCGACATCATGTATGGCGAAGGCATCTCCAAGATCGGCGAAATCCTCGACCTCGGCGTCAAGGCCGGGATCG
TCGAGAAGTCCGGCTCCTGGTTCAGCTACGACAGCATCCGGATCGGGCAGGGGCGCGAGAACGCCAAGACCTATCTCAAG
GAAAATCCCGAGATTTGCGACAAGTTGGAAGCCGCCATTCGCGGTAAAACCGACGAGGTCGCCGAGGAGATGATGACCGG
TCCTGACGCTGAGGACTGA

Upstream 100 bases:

>100_bases
AAAAAATTTCGTTCCCCCCTTGTTCCACCAGAACAAAAGCGATACATGATGTGTGTCGATAAGGGGAAATGGCCCCTAGG
CAAGCGAGGGAGACAATGCG

Downstream 100 bases:

>100_bases
GGCACGCTTCTCAAGCGGGTGCCCCACTTTGCAAAGGGCACCCGAACACATGGATCGCCGGCGCGGATGCCCCCGAAATC
CGCGCCGGCTTTTCCTTTCA

Product: recombinase A

Products: NA

Alternate protein names: Recombinase A [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MSANLKLVEKENNVDRQKALDAALAQIDRAFGKGSAMKLGSKEAMNVEAISTGSLGLDIALGIGGLPKGRVIEVYGPESS
GKTTLALHAIAEAQKAGGTAAFVDAEHALDPVYAKALGVDIDELIVSQPDTGEQALEITDTLVRSNAVDILVVDSVAALV
PRAEIEGEMGDSHVGLQARLMSQSLRKLTGSINRSKCMVIFINQLRMKIGVMYGNPETTTGGNALKFYASVRLDIRRTGQ
IKDRDEVVGNSTRVKVVKNKVAPPFKQVEFDIMYGEGISKIGEILDLGVKAGIVEKSGSWFSYDSIRIGQGRENAKTYLK
ENPEICDKLEAAIRGKTDEVAEEMMTGPDAED

Sequences:

>Translated_352_residues
MSANLKLVEKENNVDRQKALDAALAQIDRAFGKGSAMKLGSKEAMNVEAISTGSLGLDIALGIGGLPKGRVIEVYGPESS
GKTTLALHAIAEAQKAGGTAAFVDAEHALDPVYAKALGVDIDELIVSQPDTGEQALEITDTLVRSNAVDILVVDSVAALV
PRAEIEGEMGDSHVGLQARLMSQSLRKLTGSINRSKCMVIFINQLRMKIGVMYGNPETTTGGNALKFYASVRLDIRRTGQ
IKDRDEVVGNSTRVKVVKNKVAPPFKQVEFDIMYGEGISKIGEILDLGVKAGIVEKSGSWFSYDSIRIGQGRENAKTYLK
ENPEICDKLEAAIRGKTDEVAEEMMTGPDAED
>Mature_351_residues
SANLKLVEKENNVDRQKALDAALAQIDRAFGKGSAMKLGSKEAMNVEAISTGSLGLDIALGIGGLPKGRVIEVYGPESSG
KTTLALHAIAEAQKAGGTAAFVDAEHALDPVYAKALGVDIDELIVSQPDTGEQALEITDTLVRSNAVDILVVDSVAALVP
RAEIEGEMGDSHVGLQARLMSQSLRKLTGSINRSKCMVIFINQLRMKIGVMYGNPETTTGGNALKFYASVRLDIRRTGQI
KDRDEVVGNSTRVKVVKNKVAPPFKQVEFDIMYGEGISKIGEILDLGVKAGIVEKSGSWFSYDSIRIGQGRENAKTYLKE
NPEICDKLEAAIRGKTDEVAEEMMTGPDAED

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family [H]

Homologues:

Organism=Escherichia coli, GI1789051, Length=320, Percent_Identity=67.1875, Blast_Score=461, Evalue=1e-131,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR020587 [H]

Pfam domain/function: PF00154 RecA [H]

EC number: NA

Molecular weight: Translated: 37740; Mature: 37608

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00321 RECA_1 ; PS50162 RECA_2 ; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANLKLVEKENNVDRQKALDAALAQIDRAFGKGSAMKLGSKEAMNVEAISTGSLGLDIA
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEECCCCCEEEE
LGIGGLPKGRVIEVYGPESSGKTTLALHAIAEAQKAGGTAAFVDAEHALDPVYAKALGVD
ECCCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHCCCEEEEEEHHHHCCHHHHHHHCCC
IDELIVSQPDTGEQALEITDTLVRSNAVDILVVDSVAALVPRAEIEGEMGDSHVGLQARL
HHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEECHHHHHCCHHHCCCCCCCCCCCHHHHH
MSQSLRKLTGSINRSKCMVIFINQLRMKIGVMYGNPETTTGGNALKFYASVRLDIRRTGQ
HHHHHHHHHCCCCCCCEEEEEEEHHEEEEEEEECCCCCCCCCCEEEEEEEEEEEEEECCC
IKDRDEVVGNSTRVKVVKNKVAPPFKQVEFDIMYGEGISKIGEILDLGVKAGIVEKSGSW
CCCHHHHCCCCEEEEEEECCCCCCHHHEEEEEEECCCHHHHHHHHHHHHHCCEEECCCCE
FSYDSIRIGQGRENAKTYLKENPEICDKLEAAIRGKTDEVAEEMMTGPDAED
EEECCEEECCCCHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SANLKLVEKENNVDRQKALDAALAQIDRAFGKGSAMKLGSKEAMNVEAISTGSLGLDIA
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEECCCCCEEEE
LGIGGLPKGRVIEVYGPESSGKTTLALHAIAEAQKAGGTAAFVDAEHALDPVYAKALGVD
ECCCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHCCCEEEEEEHHHHCCHHHHHHHCCC
IDELIVSQPDTGEQALEITDTLVRSNAVDILVVDSVAALVPRAEIEGEMGDSHVGLQARL
HHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEECHHHHHCCHHHCCCCCCCCCCCHHHHH
MSQSLRKLTGSINRSKCMVIFINQLRMKIGVMYGNPETTTGGNALKFYASVRLDIRRTGQ
HHHHHHHHHCCCCCCCEEEEEEEHHEEEEEEEECCCCCCCCCCEEEEEEEEEEEEEECCC
IKDRDEVVGNSTRVKVVKNKVAPPFKQVEFDIMYGEGISKIGEILDLGVKAGIVEKSGSW
CCCHHHHCCCCEEEEEEECCCCCCHHHEEEEEEECCCHHHHHHHHHHHHHCCEEECCCCE
FSYDSIRIGQGRENAKTYLKENPEICDKLEAAIRGKTDEVAEEMMTGPDAED
EEECCEEECCCCHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA