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The map label for this gene is 85374319

Identifier: 85374319

GI number: 85374319

Start: 1547050

End: 1548018

Strand: Reverse

Name: 85374319

Synonym: ELI_07460

Alternate gene names: NA

Gene position: 1548018-1547050 (Counterclockwise)

Preceding gene: 85374320

Following gene: 85374318

Centisome position: 50.71

GC content: 66.98

Gene sequence:

>969_bases
ATGACCGAGCGCAATCGTGCCCCTTTGAACGTCGCCATCGTCCAGGGCCGCCCGATCCCGCTCGCCATCGGCGACGGGAT
TGCGCAGGCGACCGAGCTGGCGCGCGAGGCGGTCGAGGGCGGCGCGCAGGTGGTGGCGTTTGGGGAAACCTTCCTCGGCG
GCTATCCGCTGTGGCTCGATGAAGCGCCCGGCGCGGCGCTGTGGGATCATCCGGGCAGTAAGGCGCTGCACCGTATCCTG
CTGGAGAACGCGGTCGTGCCCAATGACGAGCGGCTGCTGGCTTTGCAGGAACTGTGCGATGCGAACGGTGCGGTGATCAG
CATTGGCGCTCACGAACGCGTGCGCAATTCGCTCTACAACAACCAGCTGACGTTCCGCCCCGGCGAGGCGGTACTCGACC
ATCGCAAGCTGGTGCCGACGCATGGCGAGCGGCTGGTATGGATGCGCGGCGATGGCTCGACCCTGGCCGTGCACCAGGCC
GAATGGGGCCGCGTCGGCAGCCTGATCTGCTGGGAGCACTGGATGCCGCTCGCCCGCGCGGCGATGCACAATCTGGGCGA
GAGCGTCCACGTCGCCGCCTGGCCAACCGTGCGCGAAGAATACGCCATCGCCAGTCGCCACTACGCGATGGAGGGGCGAT
GCTATGTGCTGGCGGCAGGGCTGGTGCAGCACAAGGAGGATGTGCTGGACGGGTTGGAGCGTTGCGGTGGTGATGCTGGG
GCGCGCGAGTTGATCGAAGCAATCCCGGCAGATGTCCTCAACAAAGGCGGCAGCCTGATCGCCGCGCCCGATGCGAGCGT
AGTCGCGCAAGCGGGCGAGGGCGAGGAGATCCTCCACGCCACACTCGACCTGTCCGCCATCGACGAAGGCCTCGCCAGCC
TCGATACCGACGGCCACTACTCGCGGCCCGATGTGTTCGAGCTGAGCGTTGATACGCGGGCCAAAGATGGGGTGAAGTGG
CGTGCGTAA

Upstream 100 bases:

>100_bases
TCCGGATCGGCAGCGCGACGATCAAGGGAGATGGCGTGGTCTTCGTTAAATGCGGATGAGCCGCTGGCGTTAGGCCTGCC
GCCGCGCTAGGCGGCAAGGC

Downstream 100 bases:

>100_bases
CCAAGCGAGCTTCGAGCAATGAATATCTGGCTCTACCTCTTGGCAGGTTTTGCGACCGTTCTGATGATAAGCGGCCTTTT
GAATATCGCTGTGAGACGAT

Product: hydrolase, carbon-nitrogen family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTERNRAPLNVAIVQGRPIPLAIGDGIAQATELAREAVEGGAQVVAFGETFLGGYPLWLDEAPGAALWDHPGSKALHRIL
LENAVVPNDERLLALQELCDANGAVISIGAHERVRNSLYNNQLTFRPGEAVLDHRKLVPTHGERLVWMRGDGSTLAVHQA
EWGRVGSLICWEHWMPLARAAMHNLGESVHVAAWPTVREEYAIASRHYAMEGRCYVLAAGLVQHKEDVLDGLERCGGDAG
ARELIEAIPADVLNKGGSLIAAPDASVVAQAGEGEEILHATLDLSAIDEGLASLDTDGHYSRPDVFELSVDTRAKDGVKW
RA

Sequences:

>Translated_322_residues
MTERNRAPLNVAIVQGRPIPLAIGDGIAQATELAREAVEGGAQVVAFGETFLGGYPLWLDEAPGAALWDHPGSKALHRIL
LENAVVPNDERLLALQELCDANGAVISIGAHERVRNSLYNNQLTFRPGEAVLDHRKLVPTHGERLVWMRGDGSTLAVHQA
EWGRVGSLICWEHWMPLARAAMHNLGESVHVAAWPTVREEYAIASRHYAMEGRCYVLAAGLVQHKEDVLDGLERCGGDAG
ARELIEAIPADVLNKGGSLIAAPDASVVAQAGEGEEILHATLDLSAIDEGLASLDTDGHYSRPDVFELSVDTRAKDGVKW
RA
>Mature_321_residues
TERNRAPLNVAIVQGRPIPLAIGDGIAQATELAREAVEGGAQVVAFGETFLGGYPLWLDEAPGAALWDHPGSKALHRILL
ENAVVPNDERLLALQELCDANGAVISIGAHERVRNSLYNNQLTFRPGEAVLDHRKLVPTHGERLVWMRGDGSTLAVHQAE
WGRVGSLICWEHWMPLARAAMHNLGESVHVAAWPTVREEYAIASRHYAMEGRCYVLAAGLVQHKEDVLDGLERCGGDAGA
RELIEAIPADVLNKGGSLIAAPDASVVAQAGEGEEILHATLDLSAIDEGLASLDTDGHYSRPDVFELSVDTRAKDGVKWR
A

Specific function: Acts on aliphatic nitriles such as acrylonitrile, crotononitrile and glutaronitrile [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17557111, Length=315, Percent_Identity=34.6031746031746, Blast_Score=156, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6322027, Length=191, Percent_Identity=34.5549738219895, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 34734; Mature: 34602

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00920 NITRIL_CHT_1 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERNRAPLNVAIVQGRPIPLAIGDGIAQATELAREAVEGGAQVVAFGETFLGGYPLWLD
CCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEECHHHHCCCCEEEE
EAPGAALWDHPGSKALHRILLENAVVPNDERLLALQELCDANGAVISIGAHERVRNSLYN
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHC
NQLTFRPGEAVLDHRKLVPTHGERLVWMRGDGSTLAVHQAEWGRVGSLICWEHWMPLARA
CEEEECCCHHHHHCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHH
AMHNLGESVHVAAWPTVREEYAIASRHYAMEGRCYVLAAGLVQHKEDVLDGLERCGGDAG
HHHHCCCCEEEEECCCHHHHHHHHHHCEEECCCEEEEEHHHHHHHHHHHHHHHHCCCCCC
ARELIEAIPADVLNKGGSLIAAPDASVVAQAGEGEEILHATLDLSAIDEGLASLDTDGHY
HHHHHHHCCHHHHHCCCCEEECCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
SRPDVFELSVDTRAKDGVKWRA
CCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TERNRAPLNVAIVQGRPIPLAIGDGIAQATELAREAVEGGAQVVAFGETFLGGYPLWLD
CCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEECHHHHCCCCEEEE
EAPGAALWDHPGSKALHRILLENAVVPNDERLLALQELCDANGAVISIGAHERVRNSLYN
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHC
NQLTFRPGEAVLDHRKLVPTHGERLVWMRGDGSTLAVHQAEWGRVGSLICWEHWMPLARA
CEEEECCCHHHHHCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHH
AMHNLGESVHVAAWPTVREEYAIASRHYAMEGRCYVLAAGLVQHKEDVLDGLERCGGDAG
HHHHCCCCEEEEECCCHHHHHHHHHHCEEECCCEEEEEHHHHHHHHHHHHHHHHCCCCCC
ARELIEAIPADVLNKGGSLIAAPDASVVAQAGEGEEILHATLDLSAIDEGLASLDTDGHY
HHHHHHHCCHHHHHCCCCEEECCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
SRPDVFELSVDTRAKDGVKWRA
CCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1390687 [H]