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The map label for this gene is rimK

Identifier: 85374305

GI number: 85374305

Start: 1534299

End: 1535204

Strand: Reverse

Name: rimK

Synonym: ELI_07390

Alternate gene names: 85374305

Gene position: 1535204-1534299 (Counterclockwise)

Preceding gene: 85374306

Following gene: 85374298

Centisome position: 50.3

GC content: 64.02

Gene sequence:

>906_bases
ATGAAAATCGCCATGCTCGCCCGCAACCCGAACCTCTATTCGCACAAGCGGCTGGTGGAGGCGGCGGAGAAGCGCGGGCA
CTCGCTCGACATTCTCAACACCACGCGCTGCACGGTGAATATCGCCAGCCACCGCCCGACGATCAGCTACAATGGGGAGA
CGATCAAGGGCTACGACGCGGTGATCCCGCGCATCGGGGCCTCGATCACCAACTACGGACTTGCCGTATTGCGCCAGTTC
GAAATGGCGGGCGTGTGGCCGCTCAACGAAAGCGTCGCCATCGGTCGCAGCCGCGACAAGCTGCGCTCCATGCAAATCCT
CGCCAAATACGGGCTGGGCCTGCCGCTCACGGCCTACGCCAACGATCCCAAGCAGGCTGAGGAGATCATCAAGGCGGTCA
ACGGCCCGCCGGTGGTCATCAAGCTGCTGGAGGGCACACAGGGCATCGGGGTGGTCCTGGCCGAGACGATGTCATCCGCC
AAGTCCGTGATCGAGGCGTTTCGCGGGGCGAACGTCAACATCCTGGTGCAGGAATTCATCAAGGAAGCGGGCGGCACCGA
TATCCGCGCGCTGGTTGTCGGCGGCAAGGTGGTCGCCGCGATGAAGCGCACCGGCGCGGCGGACGATTTCCGCAGCAACC
TCCACCGCGGCGGCAGCGCGCAGTTGATCAAGATCACGCCCGAAGAACGCTCCACCGCCGTCCGCGCGGCCAAACACATG
GGGCTTAACGTCTGCGGCGTCGACATGCTGCGCAGCAATCACGGCCCGGTGATCATGGAAGTGAACTCGTCACCGGGGCT
GGAAGGCATCGAGAAGGCGACCGAGAAGGATATCGCCGGGCAGATCATCGACTACATCGCGGCGAACGCGAAGGCGGGGA
AGACGAAGACGAAGGGGCAGGGGTGA

Upstream 100 bases:

>100_bases
TCCTGGTCGGCAGGATGGCGCTGCGCAGCCGGTTCCTCGTCGATTCGGGCCGGTCCTACCTCCAGTCGAGCGCCGAAGAG
CGCAAAAGGATACTCCACAC

Downstream 100 bases:

>100_bases
GGTTAGCGTTTTCGATCTTCAAAGGTGGCTACCAAAAACGTCGCCATTGCGCCTGCTAAATTGACGGTCAGCTCTGCGTG
ACGTGAACTCGGCTTGATCC

Product: ribosomal protein S6 modification protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKIAMLARNPNLYSHKRLVEAAEKRGHSLDILNTTRCTVNIASHRPTISYNGETIKGYDAVIPRIGASITNYGLAVLRQF
EMAGVWPLNESVAIGRSRDKLRSMQILAKYGLGLPLTAYANDPKQAEEIIKAVNGPPVVIKLLEGTQGIGVVLAETMSSA
KSVIEAFRGANVNILVQEFIKEAGGTDIRALVVGGKVVAAMKRTGAADDFRSNLHRGGSAQLIKITPEERSTAVRAAKHM
GLNVCGVDMLRSNHGPVIMEVNSSPGLEGIEKATEKDIAGQIIDYIAANAKAGKTKTKGQG

Sequences:

>Translated_301_residues
MKIAMLARNPNLYSHKRLVEAAEKRGHSLDILNTTRCTVNIASHRPTISYNGETIKGYDAVIPRIGASITNYGLAVLRQF
EMAGVWPLNESVAIGRSRDKLRSMQILAKYGLGLPLTAYANDPKQAEEIIKAVNGPPVVIKLLEGTQGIGVVLAETMSSA
KSVIEAFRGANVNILVQEFIKEAGGTDIRALVVGGKVVAAMKRTGAADDFRSNLHRGGSAQLIKITPEERSTAVRAAKHM
GLNVCGVDMLRSNHGPVIMEVNSSPGLEGIEKATEKDIAGQIIDYIAANAKAGKTKTKGQG
>Mature_301_residues
MKIAMLARNPNLYSHKRLVEAAEKRGHSLDILNTTRCTVNIASHRPTISYNGETIKGYDAVIPRIGASITNYGLAVLRQF
EMAGVWPLNESVAIGRSRDKLRSMQILAKYGLGLPLTAYANDPKQAEEIIKAVNGPPVVIKLLEGTQGIGVVLAETMSSA
KSVIEAFRGANVNILVQEFIKEAGGTDIRALVVGGKVVAAMKRTGAADDFRSNLHRGGSAQLIKITPEERSTAVRAAKHM
GLNVCGVDMLRSNHGPVIMEVNSSPGLEGIEKATEKDIAGQIIDYIAANAKAGKTKTKGQG

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI134254462, Length=250, Percent_Identity=30.8, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI164663810, Length=231, Percent_Identity=29.004329004329, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1787076, Length=290, Percent_Identity=59.6551724137931, Blast_Score=349, Evalue=1e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMK_ERYLH (Q2N9S1)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458367.1
- STRING:   Q2N9S1
- GeneID:   3871359
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_07390
- NMPDR:   fig|314225.3.peg.1565
- eggNOG:   COG0189
- HOGENOM:   HBG679591
- OMA:   CREPRLY
- PhylomeDB:   Q2N9S1
- ProtClustDB:   CLSK771613
- BioCyc:   ELIT314225:ELI_07390-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_01552
- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- TIGRFAMs:   TIGR00768

Pfam domain/function: PF08443 RimK

EC number: NA

Molecular weight: Translated: 32165; Mature: 32165

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAMLARNPNLYSHKRLVEAAEKRGHSLDILNTTRCTVNIASHRPTISYNGETIKGYDA
CEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCCCCEEEECCCEECCHHH
VIPRIGASITNYGLAVLRQFEMAGVWPLNESVAIGRSRDKLRSMQILAKYGLGLPLTAYA
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHHHHCCCCEEEECC
NDPKQAEEIIKAVNGPPVVIKLLEGTQGIGVVLAETMSSAKSVIEAFRGANVNILVQEFI
CCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHH
KEAGGTDIRALVVGGKVVAAMKRTGAADDFRSNLHRGGSAQLIKITPEERSTAVRAAKHM
HHCCCCCEEEEEECCHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHC
GLNVCGVDMLRSNHGPVIMEVNSSPGLEGIEKATEKDIAGQIIDYIAANAKAGKTKTKGQ
CCCEECHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
G
C
>Mature Secondary Structure
MKIAMLARNPNLYSHKRLVEAAEKRGHSLDILNTTRCTVNIASHRPTISYNGETIKGYDA
CEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCCCCEEEECCCEECCHHH
VIPRIGASITNYGLAVLRQFEMAGVWPLNESVAIGRSRDKLRSMQILAKYGLGLPLTAYA
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHHHHCCCCEEEECC
NDPKQAEEIIKAVNGPPVVIKLLEGTQGIGVVLAETMSSAKSVIEAFRGANVNILVQEFI
CCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHH
KEAGGTDIRALVVGGKVVAAMKRTGAADDFRSNLHRGGSAQLIKITPEERSTAVRAAKHM
HHCCCCCEEEEEECCHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHC
GLNVCGVDMLRSNHGPVIMEVNSSPGLEGIEKATEKDIAGQIIDYIAANAKAGKTKTKGQ
CCCEECHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA