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The map label for this gene is rsmB [H]

Identifier: 85374245

GI number: 85374245

Start: 1468935

End: 1470116

Strand: Reverse

Name: rsmB [H]

Synonym: ELI_07090

Alternate gene names: 85374245

Gene position: 1470116-1468935 (Counterclockwise)

Preceding gene: 85374246

Following gene: 85374244

Centisome position: 48.16

GC content: 67.6

Gene sequence:

>1182_bases
ATGACCCCCGCCGCGCGGGTTCAAGCCGCAATCGACATCCTCGACCGCGTGATCGATGCTGCGCGCAGCGAAGGTGCGCC
GGCGGACCGGATCATCGCCGACTGGGCGCGCAACAATCGGTATGCCGGAAGCAAGGACCGCCGCGCGGTGCGTGAGCTGG
TGTACAGCGCGATACGGATATGCGGCGAGGTTCCCGAAACGGGGCGGGCCGCGTTGCTGGCGCTTGCCGAAGAAGACACG
TCGCTGCGCGAATGCTTCGACGGCTCGCAATATGGACCAGAGCGGATCGGCGAAGAGGAAGGCGTGGCTTCGCGTGGAAT
CGCACCGGGCTGGCTGACAGAGGCCTTGGCGCATTCCGGCGTCTCGCACGAGGAACGAGAGGCGCTGCTGGGCCGTGCGC
CGCTCGACATTCGCATCAATACGCTGAAGGCTAGCACCGAAACGGTCGAACTGCCCCAAGATGGCGAACCGCTTGCCTTG
CCGAACGCCCTGCGTTTTGCGTCCGGCACGCAGGTCGAGCAATGGCCCGCCTATCGCGACGGCAAGATCGAGGTGCAGGA
CCTTGGCAGCCAGCTCGCCTGCCGCGCCGCAGACGCAAGGCCCGGCGAGACGGTGCTCGATCTTTGCGCCGGGGCAGGGG
GCAAGACGCTGGCGCTGGCAGCGAGCATGGAATTGCGTGGCCGGTTGATCGCCAGCGACACCGACCTGCGGCGCGGCCGA
CAATTGCTGCCCCGCGCCGAACGCGCCGGCGCCGCCGGGATCGAAACGCGCTTGCTCGATCCCGGCCGCGAGCTGGAGGT
GCTGGGCGACCTGACAGGCCAGGTCGATTGCGTGCTGGTCGATGCGCCCTGTTCCGGCACGGGCACCTGGCGGCGCAAGC
CGGAGGCGAAGTGGCGGCTCACGCCGCATGCGCTTCAGCGCTATGCCGACTTGCAGGACCACCTCCTCGATGTCGCAGCC
AGGCTCGTCAAGCCGGGCGGCCGCGTGGTCTTCGTGACCTGCTCGCTGCTCGACGCCGAGGGCCCGGATCGCTTCGAAGC
GTTTCTGGTGGGGAATTCTCGATGGGAGGCCGATATCCCCGAGTTGCCGCTCGGCCGTCAACGCGGGAAAGGTATCCGGC
TCTCGCCGTTCCATGACGGTACGGACGGATTCTTCATCGCCCGTGCAGTCTTGCCGTGCTAG

Upstream 100 bases:

>100_bases
AGCCACGTCCACGACGTGGCGATCACGCGGGAAGCGCCGAATTATCCCACACGATGACATCCATCATGTCATCGTGTGAG
TCCTCGCGAAGGACAAGCGC

Downstream 100 bases:

>100_bases
CCCCGCCGGGTATGGTTGATGGACCGTCATCCGCGCCACCTGCGAGTCTTCGCGTGAAGGAATTGCTTATGCGTTTCGCC
CCAGCCGCCGCTGCCCTTTC

Product: tRNA and rRNA cytosine-C5-methylase

Products: NA

Alternate protein names: rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MTPAARVQAAIDILDRVIDAARSEGAPADRIIADWARNNRYAGSKDRRAVRELVYSAIRICGEVPETGRAALLALAEEDT
SLRECFDGSQYGPERIGEEEGVASRGIAPGWLTEALAHSGVSHEEREALLGRAPLDIRINTLKASTETVELPQDGEPLAL
PNALRFASGTQVEQWPAYRDGKIEVQDLGSQLACRAADARPGETVLDLCAGAGGKTLALAASMELRGRLIASDTDLRRGR
QLLPRAERAGAAGIETRLLDPGRELEVLGDLTGQVDCVLVDAPCSGTGTWRRKPEAKWRLTPHALQRYADLQDHLLDVAA
RLVKPGGRVVFVTCSLLDAEGPDRFEAFLVGNSRWEADIPELPLGRQRGKGIRLSPFHDGTDGFFIARAVLPC

Sequences:

>Translated_393_residues
MTPAARVQAAIDILDRVIDAARSEGAPADRIIADWARNNRYAGSKDRRAVRELVYSAIRICGEVPETGRAALLALAEEDT
SLRECFDGSQYGPERIGEEEGVASRGIAPGWLTEALAHSGVSHEEREALLGRAPLDIRINTLKASTETVELPQDGEPLAL
PNALRFASGTQVEQWPAYRDGKIEVQDLGSQLACRAADARPGETVLDLCAGAGGKTLALAASMELRGRLIASDTDLRRGR
QLLPRAERAGAAGIETRLLDPGRELEVLGDLTGQVDCVLVDAPCSGTGTWRRKPEAKWRLTPHALQRYADLQDHLLDVAA
RLVKPGGRVVFVTCSLLDAEGPDRFEAFLVGNSRWEADIPELPLGRQRGKGIRLSPFHDGTDGFFIARAVLPC
>Mature_392_residues
TPAARVQAAIDILDRVIDAARSEGAPADRIIADWARNNRYAGSKDRRAVRELVYSAIRICGEVPETGRAALLALAEEDTS
LRECFDGSQYGPERIGEEEGVASRGIAPGWLTEALAHSGVSHEEREALLGRAPLDIRINTLKASTETVELPQDGEPLALP
NALRFASGTQVEQWPAYRDGKIEVQDLGSQLACRAADARPGETVLDLCAGAGGKTLALAASMELRGRLIASDTDLRRGRQ
LLPRAERAGAAGIETRLLDPGRELEVLGDLTGQVDCVLVDAPCSGTGTWRRKPEAKWRLTPHALQRYADLQDHLLDVAAR
LVKPGGRVVFVTCSLLDAEGPDRFEAFLVGNSRWEADIPELPLGRQRGKGIRLSPFHDGTDGFFIARAVLPC

Specific function: Unknown

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI32698918, Length=169, Percent_Identity=31.9526627218935, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI76150625, Length=244, Percent_Identity=28.2786885245902, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI76150623, Length=244, Percent_Identity=28.2786885245902, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI23199998, Length=311, Percent_Identity=27.9742765273312, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI270288818, Length=314, Percent_Identity=28.0254777070064, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI270288816, Length=241, Percent_Identity=30.2904564315353, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI8922322, Length=311, Percent_Identity=27.9742765273312, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI2367212, Length=305, Percent_Identity=30.1639344262295, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87081985, Length=167, Percent_Identity=31.7365269461078, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17536757, Length=240, Percent_Identity=30, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324268, Length=240, Percent_Identity=29.1666666666667, Blast_Score=76, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6319447, Length=220, Percent_Identity=30, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21356513, Length=264, Percent_Identity=30.3030303030303, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24668781, Length=264, Percent_Identity=30.3030303030303, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI22024126, Length=245, Percent_Identity=27.3469387755102, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: NA

Molecular weight: Translated: 42503; Mature: 42371

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAARVQAAIDILDRVIDAARSEGAPADRIIADWARNNRYAGSKDRRAVRELVYSAIRI
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
CGEVPETGRAALLALAEEDTSLRECFDGSQYGPERIGEEEGVASRGIAPGWLTEALAHSG
HCCCCCCCCEEEEEEECCCCHHHHHHCCCCCCHHHCCCCCCCHHCCCCCHHHHHHHHHCC
VSHEEREALLGRAPLDIRINTLKASTETVELPQDGEPLALPNALRFASGTQVEQWPAYRD
CCHHHHHHHHCCCCCEEEEEEEECCCCEEECCCCCCCCCCCHHHHHCCCCCHHHCCCCCC
GKIEVQDLGSQLACRAADARPGETVLDLCAGAGGKTLALAASMELRGRLIASDTDLRRGR
CEEEHHHCCCHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEECHHHCCEEEECCCCHHHHH
QLLPRAERAGAAGIETRLLDPGRELEVLGDLTGQVDCVLVDAPCSGTGTWRRKPEAKWRL
HHCCHHHHCCCCCCHHEECCCCCCEEHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCEEE
TPHALQRYADLQDHLLDVAARLVKPGGRVVFVTCSLLDAEGPDRFEAFLVGNSRWEADIP
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCCCC
ELPLGRQRGKGIRLSPFHDGTDGFFIARAVLPC
CCCCCCCCCCCCEECCCCCCCCCEEHHHHHCCC
>Mature Secondary Structure 
TPAARVQAAIDILDRVIDAARSEGAPADRIIADWARNNRYAGSKDRRAVRELVYSAIRI
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
CGEVPETGRAALLALAEEDTSLRECFDGSQYGPERIGEEEGVASRGIAPGWLTEALAHSG
HCCCCCCCCEEEEEEECCCCHHHHHHCCCCCCHHHCCCCCCCHHCCCCCHHHHHHHHHCC
VSHEEREALLGRAPLDIRINTLKASTETVELPQDGEPLALPNALRFASGTQVEQWPAYRD
CCHHHHHHHHCCCCCEEEEEEEECCCCEEECCCCCCCCCCCHHHHHCCCCCHHHCCCCCC
GKIEVQDLGSQLACRAADARPGETVLDLCAGAGGKTLALAASMELRGRLIASDTDLRRGR
CEEEHHHCCCHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEECHHHCCEEEECCCCHHHHH
QLLPRAERAGAAGIETRLLDPGRELEVLGDLTGQVDCVLVDAPCSGTGTWRRKPEAKWRL
HHCCHHHHCCCCCCHHEECCCCCCEEHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCEEE
TPHALQRYADLQDHLLDVAARLVKPGGRVVFVTCSLLDAEGPDRFEAFLVGNSRWEADIP
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCCCC
ELPLGRQRGKGIRLSPFHDGTDGFFIARAVLPC
CCCCCCCCCCCCEECCCCCCCCCEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]