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The map label for this gene is alkK [H]

Identifier: 85374222

GI number: 85374222

Start: 1445233

End: 1446831

Strand: Reverse

Name: alkK [H]

Synonym: ELI_06975

Alternate gene names: 85374222

Gene position: 1446831-1445233 (Counterclockwise)

Preceding gene: 85374223

Following gene: 85374221

Centisome position: 47.4

GC content: 62.98

Gene sequence:

>1599_bases
ATGCTCGGCCAGATGCAAGAATGGACCATGCGGATCACCCACGTGATCGACCACGCGGCGCGCGAAGCAGGGGATCGCGA
GATCGTGACGCGGTGGGCCGACGGCAGCGAAACCCGCACCGACTGGAAAGGCATCCGCACCGATGCGCTGAAAATGGCGC
AGGCGCTGCAGGCGCTCGGCATCAGGAAGGGCGACAGGGTCGCCAGCCTCGCCATGAACCACTCGCGGCACCTGGTCAGC
TGGTATGGCGTCGCCGGAATGGGCGGAGTGCTCCACACCGTGAATCCGCGCCTGTTCGAGGACCAGCTCGAATATATCGT
CAATCACGCCGAAGATAAGGTCATGCTCTATGATGCGGCGTTCCAGCCGATCATCGACAAGATGCGCGAGAAGTGGACCA
CGGTCGAACATTATATCTGTTACGATTCCGGCGAGCAGAGCCAGTCTTTTGAAGACTGGATCGGCGCGCAGGATGGCGAT
TTCGAATGGGTCACCGGCGCGGAAACCGATCCGTGCATGATCTGCTACACAAGCGGGACCACCGGCAACCCCAAGGGCGT
CCAGTACGAGCATCGCTCGACATTGATGCACGCGCTTGCCGGTCTGCAACCGGCAGCCTTCAATTTCAGCGCCAGCTCGG
TGATGCTGCCGGTCGTCCCGATGTTCCACGCTGCCAGCTGGGGCCTGCCCTATGCCGGTGCGATGGCGGGCATCAAATTC
GTCTTCAGCGCGGTCAACGATCCGGCGGTTTTGCACCAGCTGCTGATCGACGAAGGCGTGACCGACAGCGCGGGCGTCCC
GACCGTCTGGCTCGCGCATTTCCAGTATTGCGACAAGGAAGGCATCGACCTGCCGCCCTTGAAGGCTGCCACCATCGGCG
GCTCGGCCTGTCCGCGCTTCATGATCGAGCGACTGATGAAGAACGGCACCCGCGTGCAGCACGCCTGGGGCATGACCGAG
ACTTCTCCCATCGGCACGGTCGGCGGGCCGACCTGGGACTGGGACCAGCTCAGCTTCGAGGAAAAGGTCGACAAGACCGC
GATGCAGGGCCGCCCGGTCTTCGGCGTCGAATTGCGCACCGTCAGCCTCGACGACATGGTCACCGAACTGCCACGCGACG
GGGAAAGCTCGGGCGCGCTGCAGATTCGTGGGCCGTGGATCATCAAGCGTTACTTCAAGGCCGAACAGGACGCGGTCGGC
AATGACGGCTGGTTCGACACCGGCGATGTCGGCATCATCCATCCGGATGGCACTTTGCAGCTCACCGACCGCACGAAGGA
CGTGATCAAGTCCGGCGGCGAATGGATCAGCTCGGTCGAGCTGGAGAATGCGGCTTGCGGTCACCCAGCCGTCGCCGAGG
CGGCCTGTGTCGGCATGCCGCACCCGAAGTGGGACGAGCGACCGGTGCTGTTCGTGGTCAAAAAGGAAGGGCAGGAGGTC
GAGGCGGAAACGATCGTCAATTTCCTGAGCGACAAGGTGGCGAAATGGTGGCTGCCCGATGCGGTCGAGTTCGTCGACGA
CATCCCGCACACCGCGACCGGCAAGATCAGCAAGAAGGACCTGCGCGACCGGTTTGCCGATTACAAGCTCGAGGCGTGA

Upstream 100 bases:

>100_bases
AAGGCGGCCTGACGAGCGGCACTCCGCCAATTTCTCGCTTGCCGCGGGCGCTTTCCCCGCCCTATCTGGTTGCAAAGAGA
AATCGAAGGAGAGGACTTCA

Downstream 100 bases:

>100_bases
GCAATGGCGGAGCATTACATCGATCCCTCGCCCGCAAATTTCCAGGCGTTCAAGGACCTGCCGCGCGACGAGCCGATCCT
CATGCTCAACCTGCTGCGCT

Product: medium-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MLGQMQEWTMRITHVIDHAAREAGDREIVTRWADGSETRTDWKGIRTDALKMAQALQALGIRKGDRVASLAMNHSRHLVS
WYGVAGMGGVLHTVNPRLFEDQLEYIVNHAEDKVMLYDAAFQPIIDKMREKWTTVEHYICYDSGEQSQSFEDWIGAQDGD
FEWVTGAETDPCMICYTSGTTGNPKGVQYEHRSTLMHALAGLQPAAFNFSASSVMLPVVPMFHAASWGLPYAGAMAGIKF
VFSAVNDPAVLHQLLIDEGVTDSAGVPTVWLAHFQYCDKEGIDLPPLKAATIGGSACPRFMIERLMKNGTRVQHAWGMTE
TSPIGTVGGPTWDWDQLSFEEKVDKTAMQGRPVFGVELRTVSLDDMVTELPRDGESSGALQIRGPWIIKRYFKAEQDAVG
NDGWFDTGDVGIIHPDGTLQLTDRTKDVIKSGGEWISSVELENAACGHPAVAEAACVGMPHPKWDERPVLFVVKKEGQEV
EAETIVNFLSDKVAKWWLPDAVEFVDDIPHTATGKISKKDLRDRFADYKLEA

Sequences:

>Translated_532_residues
MLGQMQEWTMRITHVIDHAAREAGDREIVTRWADGSETRTDWKGIRTDALKMAQALQALGIRKGDRVASLAMNHSRHLVS
WYGVAGMGGVLHTVNPRLFEDQLEYIVNHAEDKVMLYDAAFQPIIDKMREKWTTVEHYICYDSGEQSQSFEDWIGAQDGD
FEWVTGAETDPCMICYTSGTTGNPKGVQYEHRSTLMHALAGLQPAAFNFSASSVMLPVVPMFHAASWGLPYAGAMAGIKF
VFSAVNDPAVLHQLLIDEGVTDSAGVPTVWLAHFQYCDKEGIDLPPLKAATIGGSACPRFMIERLMKNGTRVQHAWGMTE
TSPIGTVGGPTWDWDQLSFEEKVDKTAMQGRPVFGVELRTVSLDDMVTELPRDGESSGALQIRGPWIIKRYFKAEQDAVG
NDGWFDTGDVGIIHPDGTLQLTDRTKDVIKSGGEWISSVELENAACGHPAVAEAACVGMPHPKWDERPVLFVVKKEGQEV
EAETIVNFLSDKVAKWWLPDAVEFVDDIPHTATGKISKKDLRDRFADYKLEA
>Mature_532_residues
MLGQMQEWTMRITHVIDHAAREAGDREIVTRWADGSETRTDWKGIRTDALKMAQALQALGIRKGDRVASLAMNHSRHLVS
WYGVAGMGGVLHTVNPRLFEDQLEYIVNHAEDKVMLYDAAFQPIIDKMREKWTTVEHYICYDSGEQSQSFEDWIGAQDGD
FEWVTGAETDPCMICYTSGTTGNPKGVQYEHRSTLMHALAGLQPAAFNFSASSVMLPVVPMFHAASWGLPYAGAMAGIKF
VFSAVNDPAVLHQLLIDEGVTDSAGVPTVWLAHFQYCDKEGIDLPPLKAATIGGSACPRFMIERLMKNGTRVQHAWGMTE
TSPIGTVGGPTWDWDQLSFEEKVDKTAMQGRPVFGVELRTVSLDDMVTELPRDGESSGALQIRGPWIIKRYFKAEQDAVG
NDGWFDTGDVGIIHPDGTLQLTDRTKDVIKSGGEWISSVELENAACGHPAVAEAACVGMPHPKWDERPVLFVVKKEGQEV
EAETIVNFLSDKVAKWWLPDAVEFVDDIPHTATGKISKKDLRDRFADYKLEA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=557, Percent_Identity=24.2369838420108, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI187761345, Length=536, Percent_Identity=25, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI187761343, Length=536, Percent_Identity=25, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI28416953, Length=533, Percent_Identity=23.0769230769231, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI115511026, Length=484, Percent_Identity=23.3471074380165, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI42544132, Length=511, Percent_Identity=23.679060665362, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI58082049, Length=542, Percent_Identity=23.9852398523985, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI157311624, Length=540, Percent_Identity=23.8888888888889, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI157311622, Length=540, Percent_Identity=23.8888888888889, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI38505220, Length=517, Percent_Identity=24.7582205029014, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI122937307, Length=520, Percent_Identity=22.1153846153846, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI13375727, Length=538, Percent_Identity=22.8624535315985, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788107, Length=505, Percent_Identity=26.1386138613861, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI145693145, Length=486, Percent_Identity=26.7489711934156, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1786810, Length=531, Percent_Identity=26.3653483992467, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI221142682, Length=233, Percent_Identity=31.3304721030043, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790505, Length=557, Percent_Identity=24.4165170556553, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788595, Length=360, Percent_Identity=26.1111111111111, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789201, Length=362, Percent_Identity=23.7569060773481, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71994694, Length=550, Percent_Identity=24.9090909090909, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI71994703, Length=550, Percent_Identity=24.9090909090909, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI71994690, Length=550, Percent_Identity=24.9090909090909, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17559526, Length=540, Percent_Identity=25.1851851851852, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17538037, Length=520, Percent_Identity=24.6153846153846, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17557194, Length=560, Percent_Identity=24.4642857142857, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17531443, Length=532, Percent_Identity=22.1804511278195, Blast_Score=119, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17558820, Length=483, Percent_Identity=27.1221532091097, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17560308, Length=529, Percent_Identity=25.5198487712665, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI32563687, Length=513, Percent_Identity=24.5614035087719, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71985884, Length=366, Percent_Identity=26.2295081967213, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71996755, Length=482, Percent_Identity=22.8215767634855, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17560140, Length=513, Percent_Identity=20.4678362573099, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=375, Percent_Identity=28.2666666666667, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6320852, Length=489, Percent_Identity=24.7443762781186, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24581924, Length=552, Percent_Identity=25.3623188405797, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI18859661, Length=507, Percent_Identity=23.6686390532544, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI21355181, Length=526, Percent_Identity=22.8136882129278, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19922652, Length=513, Percent_Identity=24.7563352826511, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24648253, Length=508, Percent_Identity=22.0472440944882, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648255, Length=505, Percent_Identity=22.1782178217822, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21356947, Length=500, Percent_Identity=23.4, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21358303, Length=510, Percent_Identity=22.7450980392157, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24667955, Length=585, Percent_Identity=21.7094017094017, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI62472339, Length=553, Percent_Identity=22.0614828209765, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648260, Length=493, Percent_Identity=22.5152129817444, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281365686, Length=371, Percent_Identity=23.1805929919137, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24653035, Length=506, Percent_Identity=22.1343873517787, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24667959, Length=393, Percent_Identity=23.6641221374046, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356441, Length=511, Percent_Identity=20.9393346379648, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI20130357, Length=501, Percent_Identity=22.1556886227545, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 58973; Mature: 58973

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGQMQEWTMRITHVIDHAAREAGDREIVTRWADGSETRTDWKGIRTDALKMAQALQALG
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHC
IRKGDRVASLAMNHSRHLVSWYGVAGMGGVLHTVNPRLFEDQLEYIVNHAEDKVMLYDAA
CCCCCHHHHHHHCCCCHHHHHHCHHCCCCHHECCCCHHHHHHHHHHHHCCCCCEEEEHHH
FQPIIDKMREKWTTVEHYICYDSGEQSQSFEDWIGAQDGDFEWVTGAETDPCMICYTSGT
HHHHHHHHHHHHCCHHEEEEECCCCCCCCHHHHCCCCCCCCEEEECCCCCCEEEEEECCC
TGNPKGVQYEHRSTLMHALAGLQPAAFNFSASSVMLPVVPMFHAASWGLPYAGAMAGIKF
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
VFSAVNDPAVLHQLLIDEGVTDSAGVPTVWLAHFQYCDKEGIDLPPLKAATIGGSACPRF
HHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
MIERLMKNGTRVQHAWGMTETSPIGTVGGPTWDWDQLSFEEKVDKTAMQGRPVFGVELRT
HHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHCCCCEEEEEEEE
VSLDDMVTELPRDGESSGALQIRGPWIIKRYFKAEQDAVGNDGWFDTGDVGIIHPDGTLQ
EEHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCEE
LTDRTKDVIKSGGEWISSVELENAACGHPAVAEAACVGMPHPKWDERPVLFVVKKEGQEV
ECHHHHHHHHCCCHHHHHEEECCCCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCC
EAETIVNFLSDKVAKWWLPDAVEFVDDIPHTATGKISKKDLRDRFADYKLEA
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLGQMQEWTMRITHVIDHAAREAGDREIVTRWADGSETRTDWKGIRTDALKMAQALQALG
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHC
IRKGDRVASLAMNHSRHLVSWYGVAGMGGVLHTVNPRLFEDQLEYIVNHAEDKVMLYDAA
CCCCCHHHHHHHCCCCHHHHHHCHHCCCCHHECCCCHHHHHHHHHHHHCCCCCEEEEHHH
FQPIIDKMREKWTTVEHYICYDSGEQSQSFEDWIGAQDGDFEWVTGAETDPCMICYTSGT
HHHHHHHHHHHHCCHHEEEEECCCCCCCCHHHHCCCCCCCCEEEECCCCCCEEEEEECCC
TGNPKGVQYEHRSTLMHALAGLQPAAFNFSASSVMLPVVPMFHAASWGLPYAGAMAGIKF
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
VFSAVNDPAVLHQLLIDEGVTDSAGVPTVWLAHFQYCDKEGIDLPPLKAATIGGSACPRF
HHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
MIERLMKNGTRVQHAWGMTETSPIGTVGGPTWDWDQLSFEEKVDKTAMQGRPVFGVELRT
HHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHCCCCEEEEEEEE
VSLDDMVTELPRDGESSGALQIRGPWIIKRYFKAEQDAVGNDGWFDTGDVGIIHPDGTLQ
EEHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCEE
LTDRTKDVIKSGGEWISSVELENAACGHPAVAEAACVGMPHPKWDERPVLFVVKKEGQEV
ECHHHHHHHHCCCHHHHHEEECCCCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCC
EAETIVNFLSDKVAKWWLPDAVEFVDDIPHTATGKISKKDLRDRFADYKLEA
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]