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The map label for this gene is nuoB [H]

Identifier: 85374165

GI number: 85374165

Start: 1385392

End: 1386045

Strand: Reverse

Name: nuoB [H]

Synonym: ELI_06690

Alternate gene names: 85374165

Gene position: 1386045-1385392 (Counterclockwise)

Preceding gene: 85374166

Following gene: 85374164

Centisome position: 45.41

GC content: 63.15

Gene sequence:

>654_bases
ATGAGCACGCAGAATAACAACACGCGCCTCGCAGGCCTCACGCCTTCCGATGTGACCGGCGGTCAGTCGCGGGTCGATAA
TTACGGCACGATGACTGCCGATCACAATCGAGCCCTCCACGATGCACCGACCGCCAGGGGCGGCGAAGTGCGTCAGCCCG
ATGGCGATTATTTCAACGCGCTGCAGACCGAGGTCAACGACAAGGGCTTCCTCGTCACAAGCACCGAGGACCTGTTCCAG
TGGGCGCGCACCGGTTCGCTGTGGTGGATGACCTTCGGCCTGGCCTGCTGCGCGGTCGAGATGATCCACGTCAACATGCC
GCGCTACGACATGGAGCGTTTCGGCGCTGCACCACGCGCATCCCCTCGCCAGAGCGACGTGATGATCGTCGCGGGCACGC
TCTGCAACAAGATGGCCCCCGCGCTGCGCAAGGTCTACGACCAGATGTCGGACCCGAAATACGTGATCAGCATGGGTAGC
TGCGCTAATGGCGGTGGGTATTACCACTACAGCTATTCGGTCGTGCGCGGCTGCGACCGCATCGTGCCGGTCGATATCTA
TGTGCCAGGCTGCCCGCCGACCGCGGAAGCGCTGCTCTACGGCGTGATGCAGTTGCAGCGGAAGATCCGCCGCTCCGGGA
CGATCGAGCGATGA

Upstream 100 bases:

>100_bases
AGCGGATGGATCGGCTGGATCGGCATGATGATCTTCCTCGTCATCCTCACCGTGGGTTTTGCCTACGAGTGGAAGAAGGG
CGCGCTGGAGTGGGAATGAC

Downstream 100 bases:

>100_bases
GAGGCTCGCGCTAAGATGGCTGTCGTTCACTCCGCCCCCAAATATGCCTCCAACGACGGCGTGCGCGGCACGCTGGTGAA
GGCGCTGGGCGATTATGTCG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSTQNNNTRLAGLTPSDVTGGQSRVDNYGTMTADHNRALHDAPTARGGEVRQPDGDYFNALQTEVNDKGFLVTSTEDLFQ
WARTGSLWWMTFGLACCAVEMIHVNMPRYDMERFGAAPRASPRQSDVMIVAGTLCNKMAPALRKVYDQMSDPKYVISMGS
CANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGVMQLQRKIRRSGTIER

Sequences:

>Translated_217_residues
MSTQNNNTRLAGLTPSDVTGGQSRVDNYGTMTADHNRALHDAPTARGGEVRQPDGDYFNALQTEVNDKGFLVTSTEDLFQ
WARTGSLWWMTFGLACCAVEMIHVNMPRYDMERFGAAPRASPRQSDVMIVAGTLCNKMAPALRKVYDQMSDPKYVISMGS
CANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGVMQLQRKIRRSGTIER
>Mature_216_residues
STQNNNTRLAGLTPSDVTGGQSRVDNYGTMTADHNRALHDAPTARGGEVRQPDGDYFNALQTEVNDKGFLVTSTEDLFQW
ARTGSLWWMTFGLACCAVEMIHVNMPRYDMERFGAAPRASPRQSDVMIVAGTLCNKMAPALRKVYDQMSDPKYVISMGSC
ANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGVMQLQRKIRRSGTIER

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=185, Percent_Identity=65.945945945946, Blast_Score=256, Evalue=8e-69,
Organism=Escherichia coli, GI1788624, Length=152, Percent_Identity=50.6578947368421, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1789074, Length=125, Percent_Identity=43.2, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=40.9836065573771, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=143, Percent_Identity=68.5314685314685, Blast_Score=226, Evalue=7e-60,
Organism=Drosophila melanogaster, GI18859983, Length=159, Percent_Identity=69.1823899371069, Blast_Score=246, Evalue=9e-66,
Organism=Drosophila melanogaster, GI24642371, Length=159, Percent_Identity=69.1823899371069, Blast_Score=246, Evalue=9e-66,
Organism=Drosophila melanogaster, GI24651058, Length=141, Percent_Identity=75.177304964539, Blast_Score=239, Evalue=9e-64,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 24006; Mature: 23875

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQNNNTRLAGLTPSDVTGGQSRVDNYGTMTADHNRALHDAPTARGGEVRQPDGDYFNA
CCCCCCCCEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
LQTEVNDKGFLVTSTEDLFQWARTGSLWWMTFGLACCAVEMIHVNMPRYDMERFGAAPRA
HHHCCCCCCEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCC
SPRQSDVMIVAGTLCNKMAPALRKVYDQMSDPKYVISMGSCANGGGYYHYSYSVVRGCDR
CCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEHHHHHCCCC
IVPVDIYVPGCPPTAEALLYGVMQLQRKIRRSGTIER
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
STQNNNTRLAGLTPSDVTGGQSRVDNYGTMTADHNRALHDAPTARGGEVRQPDGDYFNA
CCCCCCCEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
LQTEVNDKGFLVTSTEDLFQWARTGSLWWMTFGLACCAVEMIHVNMPRYDMERFGAAPRA
HHHCCCCCCEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCC
SPRQSDVMIVAGTLCNKMAPALRKVYDQMSDPKYVISMGSCANGGGYYHYSYSVVRGCDR
CCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEHHHHHCCCC
IVPVDIYVPGCPPTAEALLYGVMQLQRKIRRSGTIER
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA