Click here to switch to the map view.

The map label for this gene is rnjA [H]

Identifier: 85374144

GI number: 85374144

Start: 1366855

End: 1368504

Strand: Reverse

Name: rnjA [H]

Synonym: ELI_06585

Alternate gene names: 85374144

Gene position: 1368504-1366855 (Counterclockwise)

Preceding gene: 85374145

Following gene: 85374143

Centisome position: 44.83

GC content: 64.12

Gene sequence:

>1650_bases
ATGAAAAAAGATTACACGCCGGAAGACGAGCTGCTGTTCCTTGCGCTCGGTGGGTCGGGCGAGATCGGCATGAACGTCAA
TCTCTACGGCTGCCAGGGCAAATGGCTGATGGTGGACCTCGGCATGACCTTCGGCGCCAACGAGTATCCGGGCACCGAGC
TGGTCTTTGCCGACCTCGAATTTATCGAGGAGCGCCGCAAGGACCTGCTGGGCGTGGTGCTGACACATGCGCACGAGGAT
CACATCGGCGCGGTGCCCTATTTCGCCGAGGAATTGGGCGTTCCGCTCTATGCCACGCCGTTCACCGCCGAGCTGGTGCT
GCGCAAGCTCGAAGAGGCCGGGCTCGACACCGCAATCGAGATCAACATCATCGAGGAAGATCACGGCGAGATCGAGCTGG
GTCCGTTCACCATCACCTATCTGCCGCTCGCGCACTCGATTGCCGAAGGCAATGCGCTGCTGATCGACACGCCGCACGGC
CGCATCTTCCACACCGGCGACTGGAAGCTCGACGAGGATCCGATCATCGGCGAGCCGACTACGGAAGAGGAATTGCGCGA
AATTGGAGACGAGGGCGTGCTGGCGCTGGTCTGCGACAGCACCAATGTCTTCAATCCCAAATCCAGCGGCTCGGAAGGCG
CTGTTTACAAGGGCTTGCTCGATGAAGTTCGCAAATGGGACGGGCGGCGCGTGCTGGTAACGACCTTCGCCTCCAATGTC
GCTCGGCTGCAGACCTTGGGCGAAGTCGCAAAAGAAACCGGGCGGGCGCTGTGCGTGGCCGGTCGGTCGATCGAGCGCAT
CATCGACGTCTCCAAGCAGTCCGGCTATCTCGCCGACTTCCCCGAACTGGTGGATTGGGACACGGCGATGGGGCTGCCGC
GCGGGCAGGTCATGATCCTCGCCACCGGAGGGCAGGGCGAGCCGCGCGCGGCGCTCAGCCGGATTGCCGAGCAAAACCAT
CCCATTGAGCTCACCAGTGGCGATGTCGTGATGTTTTCCAGCCGCCAGATCCCGGGCAACGAGATCGGCATCGGCAAGGT
CCAGAACCGCTTGGCCGAGCGCGGCATCGTGATGGTAACCGACCGGCAGAGCATGATCCACGTTTCAGGGCATCCTGGCC
GGCCTGAGCTGGCCGCGCTCTACGGATGGCTGCGGCCCGATATCCTCGTGCCGGTCCATGGCGAAATCCGCCACATGCAG
GAGCAGTGCCGCTTCGGGAAATCCGAGGGCATCGAACACTGCACCTTCCAGTCCAATGGCGACATCGTGCGCCTTGCGCC
CGGCGCGTCGGGCAGGGTGGCGCAGGTCCATACCGGTCGCCTTGTGCTGGATGGCGACATCATCGTGCCCGCCGACGGCG
AAAGCATCGTCATGCGGCGCAGGCTGGCCTTCAACGGGGTGGTCATGGTCGTGCTCGATCACAAGGGCGGGCCGCAGGTG
GAAGCTTTGGGGCTGCCGCTCGACGAGGACTACGCGGACTTTGTCGAGGAAGCGCAGGCGGACATCGTCGAGGCGTTGCG
CCGATTGAAAGGCAAGGCGGCGAGCGACCGCGATGCCGTGATCGAAGCCGCCCGCCTTGCGACCCGCCGCGCAGCGACGC
GCTGGTCGGGCAAGCGCCCGCAGGTCAAGGTCATGCTGGCGGAGGGCTGA

Upstream 100 bases:

>100_bases
TTCGACGAGAAAACCGACATTTTCGACCACGTCGATGCGGACCTTACCATCGAGGGTCTCGCGATCCTCGCCGAACACGC
ATCGAAGCAATAAGAGACCT

Downstream 100 bases:

>100_bases
GCCGTGGCATGGACCTCTATTCTCGCGGTCTATGCCCTGTTCTGGGTCATGAGCGCGTTCATCCTGCTGCCCTTCGGCGT
GAAAACGGCCGATGAGGCAG

Product: metallo-beta-lactamase hydrolase subunit

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MKKDYTPEDELLFLALGGSGEIGMNVNLYGCQGKWLMVDLGMTFGANEYPGTELVFADLEFIEERRKDLLGVVLTHAHED
HIGAVPYFAEELGVPLYATPFTAELVLRKLEEAGLDTAIEINIIEEDHGEIELGPFTITYLPLAHSIAEGNALLIDTPHG
RIFHTGDWKLDEDPIIGEPTTEEELREIGDEGVLALVCDSTNVFNPKSSGSEGAVYKGLLDEVRKWDGRRVLVTTFASNV
ARLQTLGEVAKETGRALCVAGRSIERIIDVSKQSGYLADFPELVDWDTAMGLPRGQVMILATGGQGEPRAALSRIAEQNH
PIELTSGDVVMFSSRQIPGNEIGIGKVQNRLAERGIVMVTDRQSMIHVSGHPGRPELAALYGWLRPDILVPVHGEIRHMQ
EQCRFGKSEGIEHCTFQSNGDIVRLAPGASGRVAQVHTGRLVLDGDIIVPADGESIVMRRRLAFNGVVMVVLDHKGGPQV
EALGLPLDEDYADFVEEAQADIVEALRRLKGKAASDRDAVIEAARLATRRAATRWSGKRPQVKVMLAEG

Sequences:

>Translated_549_residues
MKKDYTPEDELLFLALGGSGEIGMNVNLYGCQGKWLMVDLGMTFGANEYPGTELVFADLEFIEERRKDLLGVVLTHAHED
HIGAVPYFAEELGVPLYATPFTAELVLRKLEEAGLDTAIEINIIEEDHGEIELGPFTITYLPLAHSIAEGNALLIDTPHG
RIFHTGDWKLDEDPIIGEPTTEEELREIGDEGVLALVCDSTNVFNPKSSGSEGAVYKGLLDEVRKWDGRRVLVTTFASNV
ARLQTLGEVAKETGRALCVAGRSIERIIDVSKQSGYLADFPELVDWDTAMGLPRGQVMILATGGQGEPRAALSRIAEQNH
PIELTSGDVVMFSSRQIPGNEIGIGKVQNRLAERGIVMVTDRQSMIHVSGHPGRPELAALYGWLRPDILVPVHGEIRHMQ
EQCRFGKSEGIEHCTFQSNGDIVRLAPGASGRVAQVHTGRLVLDGDIIVPADGESIVMRRRLAFNGVVMVVLDHKGGPQV
EALGLPLDEDYADFVEEAQADIVEALRRLKGKAASDRDAVIEAARLATRRAATRWSGKRPQVKVMLAEG
>Mature_549_residues
MKKDYTPEDELLFLALGGSGEIGMNVNLYGCQGKWLMVDLGMTFGANEYPGTELVFADLEFIEERRKDLLGVVLTHAHED
HIGAVPYFAEELGVPLYATPFTAELVLRKLEEAGLDTAIEINIIEEDHGEIELGPFTITYLPLAHSIAEGNALLIDTPHG
RIFHTGDWKLDEDPIIGEPTTEEELREIGDEGVLALVCDSTNVFNPKSSGSEGAVYKGLLDEVRKWDGRRVLVTTFASNV
ARLQTLGEVAKETGRALCVAGRSIERIIDVSKQSGYLADFPELVDWDTAMGLPRGQVMILATGGQGEPRAALSRIAEQNH
PIELTSGDVVMFSSRQIPGNEIGIGKVQNRLAERGIVMVTDRQSMIHVSGHPGRPELAALYGWLRPDILVPVHGEIRHMQ
EQCRFGKSEGIEHCTFQSNGDIVRLAPGASGRVAQVHTGRLVLDGDIIVPADGESIVMRRRLAFNGVVMVVLDHKGGPQV
EALGLPLDEDYADFVEEAQADIVEALRRLKGKAASDRDAVIEAARLATRRAATRWSGKRPQVKVMLAEG

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 59970; Mature: 59970

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDYTPEDELLFLALGGSGEIGMNVNLYGCQGKWLMVDLGMTFGANEYPGTELVFADLE
CCCCCCCCCCEEEEEECCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCCCEEEEHHHH
FIEERRKDLLGVVLTHAHEDHIGAVPYFAEELGVPLYATPFTAELVLRKLEEAGLDTAIE
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEECCCHHHHHHHHHHHCCCCEEEE
INIIEEDHGEIELGPFTITYLPLAHSIAEGNALLIDTPHGRIFHTGDWKLDEDPIIGEPT
EEEEECCCCCEEECCEEEEEEHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCC
TEEELREIGDEGVLALVCDSTNVFNPKSSGSEGAVYKGLLDEVRKWDGRRVLVTTFASNV
CHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHH
ARLQTLGEVAKETGRALCVAGRSIERIIDVSKQSGYLADFPELVDWDTAMGLPRGQVMIL
HHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCHHHHCCHHHCCCCCCCEEEE
ATGGQGEPRAALSRIAEQNHPIELTSGDVVMFSSRQIPGNEIGIGKVQNRLAERGIVMVT
EECCCCCHHHHHHHHHHCCCCEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEE
DRQSMIHVSGHPGRPELAALYGWLRPDILVPVHGEIRHMQEQCRFGKSEGIEHCTFQSNG
CCCCEEEECCCCCCCHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCCCCCEEEECCCC
DIVRLAPGASGRVAQVHTGRLVLDGDIIVPADGESIVMRRRLAFNGVVMVVLDHKGGPQV
CEEEECCCCCCCEEEEECCEEEEECCEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCE
EALGLPLDEDYADFVEEAQADIVEALRRLKGKAASDRDAVIEAARLATRRAATRWSGKRP
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
QVKVMLAEG
EEEEEEECC
>Mature Secondary Structure
MKKDYTPEDELLFLALGGSGEIGMNVNLYGCQGKWLMVDLGMTFGANEYPGTELVFADLE
CCCCCCCCCCEEEEEECCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCCCEEEEHHHH
FIEERRKDLLGVVLTHAHEDHIGAVPYFAEELGVPLYATPFTAELVLRKLEEAGLDTAIE
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEECCCHHHHHHHHHHHCCCCEEEE
INIIEEDHGEIELGPFTITYLPLAHSIAEGNALLIDTPHGRIFHTGDWKLDEDPIIGEPT
EEEEECCCCCEEECCEEEEEEHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCC
TEEELREIGDEGVLALVCDSTNVFNPKSSGSEGAVYKGLLDEVRKWDGRRVLVTTFASNV
CHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHH
ARLQTLGEVAKETGRALCVAGRSIERIIDVSKQSGYLADFPELVDWDTAMGLPRGQVMIL
HHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCHHHHCCHHHCCCCCCCEEEE
ATGGQGEPRAALSRIAEQNHPIELTSGDVVMFSSRQIPGNEIGIGKVQNRLAERGIVMVT
EECCCCCHHHHHHHHHHCCCCEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEE
DRQSMIHVSGHPGRPELAALYGWLRPDILVPVHGEIRHMQEQCRFGKSEGIEHCTFQSNG
CCCCEEEECCCCCCCHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCCCCCEEEECCCC
DIVRLAPGASGRVAQVHTGRLVLDGDIIVPADGESIVMRRRLAFNGVVMVVLDHKGGPQV
CEEEECCCCCCCEEEEECCEEEEECCEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCE
EALGLPLDEDYADFVEEAQADIVEALRRLKGKAASDRDAVIEAARLATRRAATRWSGKRP
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
QVKVMLAEG
EEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]