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The map label for this gene is tpiA [H]

Identifier: 85374121

GI number: 85374121

Start: 1342716

End: 1343480

Strand: Reverse

Name: tpiA [H]

Synonym: ELI_06470

Alternate gene names: 85374121

Gene position: 1343480-1342716 (Counterclockwise)

Preceding gene: 85374122

Following gene: 85374120

Centisome position: 44.01

GC content: 64.18

Gene sequence:

>765_bases
ATGTCTCTCCGACCCTATATCGTCGGCAATTGGAAAATGAACGGAACCCGCGCGATGCTCTCTGAAGCTCGTGCGATCGA
CCGTGCGGCGCAGCGCTACATGAAGGTCGAGGTCGCCGTGGCGCCGCCGTTCACGCTCATCCACGCCGTCCATCGCGAAG
CCGAGCAGATCGCTGTCGGTGCACAGGATTGCCATGCCGATCCCGACGGCGCGCATACAGGCGACATTTCCGCGACCATG
GCCGCCGATGCCGGCGCGAAATTCGTGATCCTGGGCCATAGCGAACGCCGCCAGGACCACGGCGAATCCAACGAACTGGT
CCGGAGCAAGGCCGAGGCGGCGCTGGAAGCCGGGCTCAAGGTCATCCTGTGCTGCGGCGAACCCGAGGAAAAGCGCGACG
AGGGCAAGGCCGAGGAGTTCGTCCTCACCCAGCTCAAGCTGTCGCTGCCGGATGCGATGGAAGATGTCGTCGAGCGTTTG
ACCATTGCCTACGAACCGATCTGGGCGATCGGCACCGGGCGTACGCCGACGCTGGACGATATTGCTGCCATGCACGGGGC
GATCCGCGCGTTTCTCGTCGAGCGATTCGGCGAAGAAGACGGCGGACAGATTCGCATCCTCTATGGCGGTTCGGTAAAGC
CTGAAAACGCGCGCGAAATCCTCGGCGTGGCCAATGTCGGCGGCGCGCTGGTGGGCGGGGCGAGCCTGACGGCGGACAGC
TTCATGAATATCGCGCTCGCGGCCGGAGACGCCGAAGAGCAGTAG

Upstream 100 bases:

>100_bases
AACAGGTGGCAAAGGGTTTTGACTGGATCGAATTGCTCGGCTAGCGGACCCGGCCCCCACGCGCTAACCCCTTGCGCGAA
CTGACTATCAGGATTCTTGC

Downstream 100 bases:

>100_bases
GGCACGCAGCGCCGCGCATCTTGTGCCAGGGCCGCCGCGCCCCTAAATGCGCGGGTAATTGATCATTATCGGGCAAACAT
CATGTCGCTCTTTCTTTTCC

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSLRPYIVGNWKMNGTRAMLSEARAIDRAAQRYMKVEVAVAPPFTLIHAVHREAEQIAVGAQDCHADPDGAHTGDISATM
AADAGAKFVILGHSERRQDHGESNELVRSKAEAALEAGLKVILCCGEPEEKRDEGKAEEFVLTQLKLSLPDAMEDVVERL
TIAYEPIWAIGTGRTPTLDDIAAMHGAIRAFLVERFGEEDGGQIRILYGGSVKPENAREILGVANVGGALVGGASLTADS
FMNIALAAGDAEEQ

Sequences:

>Translated_254_residues
MSLRPYIVGNWKMNGTRAMLSEARAIDRAAQRYMKVEVAVAPPFTLIHAVHREAEQIAVGAQDCHADPDGAHTGDISATM
AADAGAKFVILGHSERRQDHGESNELVRSKAEAALEAGLKVILCCGEPEEKRDEGKAEEFVLTQLKLSLPDAMEDVVERL
TIAYEPIWAIGTGRTPTLDDIAAMHGAIRAFLVERFGEEDGGQIRILYGGSVKPENAREILGVANVGGALVGGASLTADS
FMNIALAAGDAEEQ
>Mature_253_residues
SLRPYIVGNWKMNGTRAMLSEARAIDRAAQRYMKVEVAVAPPFTLIHAVHREAEQIAVGAQDCHADPDGAHTGDISATMA
ADAGAKFVILGHSERRQDHGESNELVRSKAEAALEAGLKVILCCGEPEEKRDEGKAEEFVLTQLKLSLPDAMEDVVERLT
IAYEPIWAIGTGRTPTLDDIAAMHGAIRAFLVERFGEEDGGQIRILYGGSVKPENAREILGVANVGGALVGGASLTADSF
MNIALAAGDAEEQ

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4507645, Length=244, Percent_Identity=40.5737704918033, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI226529917, Length=244, Percent_Identity=40.5737704918033, Blast_Score=168, Evalue=4e-42,
Organism=Escherichia coli, GI1790353, Length=253, Percent_Identity=41.1067193675889, Blast_Score=173, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17536593, Length=245, Percent_Identity=41.6326530612245, Blast_Score=175, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6320255, Length=246, Percent_Identity=38.6178861788618, Blast_Score=158, Evalue=6e-40,
Organism=Drosophila melanogaster, GI28572008, Length=245, Percent_Identity=40, Blast_Score=164, Evalue=6e-41,
Organism=Drosophila melanogaster, GI28572006, Length=245, Percent_Identity=40, Blast_Score=164, Evalue=6e-41,
Organism=Drosophila melanogaster, GI28572004, Length=245, Percent_Identity=40, Blast_Score=163, Evalue=9e-41,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 27147; Mature: 27016

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRPYIVGNWKMNGTRAMLSEARAIDRAAQRYMKVEVAVAPPFTLIHAVHREAEQIAVG
CCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHCCC
AQDCHADPDGAHTGDISATMAADAGAKFVILGHSERRQDHGESNELVRSKAEAALEAGLK
HHHCCCCCCCCCCCCCEEEEECCCCCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHCCCE
VILCCGEPEEKRDEGKAEEFVLTQLKLSLPDAMEDVVERLTIAYEPIWAIGTGRTPTLDD
EEEECCCCHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHH
IAAMHGAIRAFLVERFGEEDGGQIRILYGGSVKPENAREILGVANVGGALVGGASLTADS
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHCCCCHHHHHH
FMNIALAAGDAEEQ
HHEEEEECCCCCCC
>Mature Secondary Structure 
SLRPYIVGNWKMNGTRAMLSEARAIDRAAQRYMKVEVAVAPPFTLIHAVHREAEQIAVG
CCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHCCC
AQDCHADPDGAHTGDISATMAADAGAKFVILGHSERRQDHGESNELVRSKAEAALEAGLK
HHHCCCCCCCCCCCCCEEEEECCCCCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHCCCE
VILCCGEPEEKRDEGKAEEFVLTQLKLSLPDAMEDVVERLTIAYEPIWAIGTGRTPTLDD
EEEECCCCHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHH
IAAMHGAIRAFLVERFGEEDGGQIRILYGGSVKPENAREILGVANVGGALVGGASLTADS
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHCCCCHHHHHH
FMNIALAAGDAEEQ
HHEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA