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The map label for this gene is lipA

Identifier: 85374089

GI number: 85374089

Start: 1311603

End: 1312565

Strand: Reverse

Name: lipA

Synonym: ELI_06310

Alternate gene names: 85374089

Gene position: 1312565-1311603 (Counterclockwise)

Preceding gene: 85374094

Following gene: 85374088

Centisome position: 43.0

GC content: 63.14

Gene sequence:

>963_bases
ATGGGCGGCATGAACGATCTCTCCTCCACGCCCGCCCCGGAAGGGGATCGCCCGGCGCGCCAGCGCAAGCCGGACTGGAT
CCGGGTCAAGGCGCCTGTCAGCAAGGGCTATCACGAGACGCGCAAGCTGATGCGCGAACTCAACCTCAATACGGTGTGCG
AGGAAGCGGCCTGCCCCAATATCGGCGAGTGCTGGACCAAGAAGCACGCGACGGTGATGATCCTCGGTGACGTATGCACG
CGCGCCTGCGCCTTCTGCAACGTCAAGACCGGTATGCCGCGGATCGTCGATCCGATGGAGCCCGAGAATACGGCCATCGC
GGCGGCAAAGATGGGGCTCCAGCACATCGTCATCACCAGCGTCGATCGCGACGACCTGCCCGATGGTGGCGCGGGACAGT
TTGTGAAAGTGATCGAGGCACTGCGCCGCGAGACTCCCGATACCACAATCGAGATATTGACCCCCGATTTCCGCGGCAAG
ATGCGCGCTGCCGTGGAGGCAATTTGCGAAGCCGGGCCGGACGTTTACAACCATAACCTCGAAACCGTGCCGCGGCTTTA
CCCGACGATCCGCCCGGGTGCGCGCTATTACGCGTCGCTGCGCCTGCTTGAAGAAGTGAAGAGCCACGATCCGATGATCT
TCACCAAGTCGGGGATCATGCTCGGCCTGGGCGAGCAGCGGCTCGAGGTTCATCAGGTCATGGATGATATGCGCAGCGCC
GATGTCGACTTCATCACCATGGGGCAATATCTTCAGCCGACGCCCAAGCATGCCAAGGTGGAGGATTTCGTGACCCCGAA
GGCATTCGACGCGTTCGGTGCCATCGCCCGTGCCAAGGGGTTCCTGCAGGTCGCCTCCAGCCCGTTGACCCGGTCGAGCT
ATCACGCCGGCGACGATTTCGCCGAGATGCGCGCAGCGCGCGAAGCGAAGCTCGCGAAGGAGCGCGAAAGGGCGCAGGGT
TAG

Upstream 100 bases:

>100_bases
CAACTTCGATTGCGGTGGAAGCTATCGCGCGGATTGAGAATAGCTATGCGGACCGGAACGGCAGCCCCGTACCATTGCGT
GGTACACCCAGTTTCCCTAG

Downstream 100 bases:

>100_bases
TGCCGGGCATTCGCGACAAGCGGCACCTGCCTTACACGCCCGAGCAGATGTACGATCTGGTGGCCGATGTCAGTCGCTAC
GAGGAGTTCCTGCCGTGGGT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MGGMNDLSSTPAPEGDRPARQRKPDWIRVKAPVSKGYHETRKLMRELNLNTVCEEAACPNIGECWTKKHATVMILGDVCT
RACAFCNVKTGMPRIVDPMEPENTAIAAAKMGLQHIVITSVDRDDLPDGGAGQFVKVIEALRRETPDTTIEILTPDFRGK
MRAAVEAICEAGPDVYNHNLETVPRLYPTIRPGARYYASLRLLEEVKSHDPMIFTKSGIMLGLGEQRLEVHQVMDDMRSA
DVDFITMGQYLQPTPKHAKVEDFVTPKAFDAFGAIARAKGFLQVASSPLTRSSYHAGDDFAEMRAAREAKLAKERERAQG

Sequences:

>Translated_320_residues
MGGMNDLSSTPAPEGDRPARQRKPDWIRVKAPVSKGYHETRKLMRELNLNTVCEEAACPNIGECWTKKHATVMILGDVCT
RACAFCNVKTGMPRIVDPMEPENTAIAAAKMGLQHIVITSVDRDDLPDGGAGQFVKVIEALRRETPDTTIEILTPDFRGK
MRAAVEAICEAGPDVYNHNLETVPRLYPTIRPGARYYASLRLLEEVKSHDPMIFTKSGIMLGLGEQRLEVHQVMDDMRSA
DVDFITMGQYLQPTPKHAKVEDFVTPKAFDAFGAIARAKGFLQVASSPLTRSSYHAGDDFAEMRAAREAKLAKERERAQG
>Mature_319_residues
GGMNDLSSTPAPEGDRPARQRKPDWIRVKAPVSKGYHETRKLMRELNLNTVCEEAACPNIGECWTKKHATVMILGDVCTR
ACAFCNVKTGMPRIVDPMEPENTAIAAAKMGLQHIVITSVDRDDLPDGGAGQFVKVIEALRRETPDTTIEILTPDFRGKM
RAAVEAICEAGPDVYNHNLETVPRLYPTIRPGARYYASLRLLEEVKSHDPMIFTKSGIMLGLGEQRLEVHQVMDDMRSAD
VDFITMGQYLQPTPKHAKVEDFVTPKAFDAFGAIARAKGFLQVASSPLTRSSYHAGDDFAEMRAAREAKLAKERERAQG

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=295, Percent_Identity=47.1186440677966, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI37577164, Length=249, Percent_Identity=48.5943775100402, Blast_Score=246, Evalue=2e-65,
Organism=Escherichia coli, GI1786846, Length=282, Percent_Identity=49.290780141844, Blast_Score=277, Evalue=6e-76,
Organism=Caenorhabditis elegans, GI32564533, Length=297, Percent_Identity=46.4646464646465, Blast_Score=265, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6324770, Length=289, Percent_Identity=47.0588235294118, Blast_Score=276, Evalue=3e-75,
Organism=Drosophila melanogaster, GI221513272, Length=288, Percent_Identity=48.2638888888889, Blast_Score=275, Evalue=3e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_ERYLH (Q2NAD7)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458151.1
- ProteinModelPortal:   Q2NAD7
- STRING:   Q2NAD7
- GeneID:   3871181
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_06310
- NMPDR:   fig|314225.3.peg.1794
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- PhylomeDB:   Q2NAD7
- ProtClustDB:   PRK05481
- BioCyc:   ELIT314225:ELI_06310-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 35443; Mature: 35312

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGMNDLSSTPAPEGDRPARQRKPDWIRVKAPVSKGYHETRKLMRELNLNTVCEEAACPN
CCCCCCCCCCCCCCCCCCHHHCCCCEEEEECCHHCCHHHHHHHHHHCCHHHHHHHHCCCC
IGECWTKKHATVMILGDVCTRACAFCNVKTGMPRIVDPMEPENTAIAAAKMGLQHIVITS
HHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCHHEEEEEE
VDRDDLPDGGAGQFVKVIEALRRETPDTTIEILTPDFRGKMRAAVEAICEAGPDVYNHNL
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCCHHHCCCH
ETVPRLYPTIRPGARYYASLRLLEEVKSHDPMIFTKSGIMLGLGEQRLEVHQVMDDMRSA
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCEECCCHHHHHHHHHHHHHHHC
DVDFITMGQYLQPTPKHAKVEDFVTPKAFDAFGAIARAKGFLQVASSPLTRSSYHAGDDF
CCCEEECCHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
AEMRAAREAKLAKERERAQG
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GGMNDLSSTPAPEGDRPARQRKPDWIRVKAPVSKGYHETRKLMRELNLNTVCEEAACPN
CCCCCCCCCCCCCCCCCHHHCCCCEEEEECCHHCCHHHHHHHHHHCCHHHHHHHHCCCC
IGECWTKKHATVMILGDVCTRACAFCNVKTGMPRIVDPMEPENTAIAAAKMGLQHIVITS
HHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCHHEEEEEE
VDRDDLPDGGAGQFVKVIEALRRETPDTTIEILTPDFRGKMRAAVEAICEAGPDVYNHNL
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCCHHHCCCH
ETVPRLYPTIRPGARYYASLRLLEEVKSHDPMIFTKSGIMLGLGEQRLEVHQVMDDMRSA
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCEECCCHHHHHHHHHHHHHHHC
DVDFITMGQYLQPTPKHAKVEDFVTPKAFDAFGAIARAKGFLQVASSPLTRSSYHAGDDF
CCCEEECCHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
AEMRAAREAKLAKERERAQG
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA