Click here to switch to the map view.

The map label for this gene is rph

Identifier: 85374029

GI number: 85374029

Start: 1243420

End: 1244133

Strand: Reverse

Name: rph

Synonym: ELI_06010

Alternate gene names: 85374029

Gene position: 1244133-1243420 (Counterclockwise)

Preceding gene: 85374032

Following gene: 85374028

Centisome position: 40.76

GC content: 64.01

Gene sequence:

>714_bases
ATGCGACCTTCCGGACGCGCGCCCGACGAGATGCGCGCTATCACCATCGAGACCGGCTACACCAAGCATGCCGAAGGCTC
CTGCCTGATCGGCTTCGGCGACACCAAGGTGCTGTGCACCGCCAGCGTCGAAGAACGCTTGCCGCCGTGGCTGCGCGGCA
AGGGACAGGGTTGGGTCACCGGCGAATACTCCATGCTGCCACGCGCGACGCATACTCGCGGCAACCGCGAGGCCGCACGC
GGCAAGCAGTCCGGGAGAACGCAGGAAATTCAGCGCCTTATCGGGCGCAGCTTGCGGGCCGTGGTCGATCTGGAAAAGCT
CGGCGAACGTCAGATCACGCTCGATTGCGACGTTATCCAGGCCGATGGTGGCACGCGCACCGCGTCGATCTCCGGCGCGT
GGATTGCGCTTCGCCTCGCCGTGAGCGGTTTGATGGAGCAAGGGCAAATCAAGGACGACCCCATCACCGGCAAGATCGCC
GCAATCAGCTGCGGGATCTACAATGGCACGCCGGTGCTCGATCTCGACTATGCGGAAGACTCCAACGCCGATGCCGATGC
CAATTTCGTGCTGATCGAAGGCGGCAAGATCGCAGAAGCCCAGGCCACGGCCGAAGGCGCGACCTATGACGAGGAAGGCT
TGATGCGCCTGCTCCGCCTCGCCCAGATCGGTTGCGCGCAGATCTTCAAGTCGCAGGACGAGGCGACGAAGTGA

Upstream 100 bases:

>100_bases
CTTGTCGAAGTCCCGTTCTTCCTTATAGCGCCGCTCAAGAAGTGAGGACGACCCTTCGACAAGCTCAGGGCGAACGGTTC
GTCGCAAGTGGAGAATTTTG

Downstream 100 bases:

>100_bases
CGCGCCGCCTCGGCTCAGGCTCGCTGGTCATTGCCACGCACAACGCGGGCAAGCTGAAAGAGATTTCAGCGCTGCTGGAG
CCGTATGGCGTCAAATGCAT

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MRPSGRAPDEMRAITIETGYTKHAEGSCLIGFGDTKVLCTASVEERLPPWLRGKGQGWVTGEYSMLPRATHTRGNREAAR
GKQSGRTQEIQRLIGRSLRAVVDLEKLGERQITLDCDVIQADGGTRTASISGAWIALRLAVSGLMEQGQIKDDPITGKIA
AISCGIYNGTPVLDLDYAEDSNADADANFVLIEGGKIAEAQATAEGATYDEEGLMRLLRLAQIGCAQIFKSQDEATK

Sequences:

>Translated_237_residues
MRPSGRAPDEMRAITIETGYTKHAEGSCLIGFGDTKVLCTASVEERLPPWLRGKGQGWVTGEYSMLPRATHTRGNREAAR
GKQSGRTQEIQRLIGRSLRAVVDLEKLGERQITLDCDVIQADGGTRTASISGAWIALRLAVSGLMEQGQIKDDPITGKIA
AISCGIYNGTPVLDLDYAEDSNADADANFVLIEGGKIAEAQATAEGATYDEEGLMRLLRLAQIGCAQIFKSQDEATK
>Mature_237_residues
MRPSGRAPDEMRAITIETGYTKHAEGSCLIGFGDTKVLCTASVEERLPPWLRGKGQGWVTGEYSMLPRATHTRGNREAAR
GKQSGRTQEIQRLIGRSLRAVVDLEKLGERQITLDCDVIQADGGTRTASISGAWIALRLAVSGLMEQGQIKDDPITGKIA
AISCGIYNGTPVLDLDYAEDSNADADANFVLIEGGKIAEAQATAEGATYDEEGLMRLLRLAQIGCAQIFKSQDEATK

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=209, Percent_Identity=61.7224880382775, Blast_Score=272, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI71981632, Length=187, Percent_Identity=29.4117647058824, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_ERYLH (Q2NAJ7)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458091.1
- ProteinModelPortal:   Q2NAJ7
- SMR:   Q2NAJ7
- STRING:   Q2NAJ7
- GeneID:   3870753
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_06010
- NMPDR:   fig|314225.3.peg.1858
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   Q2NAJ7
- ProtClustDB:   PRK00173
- BioCyc:   ELIT314225:ELI_06010-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25513; Mature: 25513

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSGRAPDEMRAITIETGYTKHAEGSCLIGFGDTKVLCTASVEERLPPWLRGKGQGWVT
CCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCEEEEEECHHHHCCCCCCCCCCEEEE
GEYSMLPRATHTRGNREAARGKQSGRTQEIQRLIGRSLRAVVDLEKLGERQITLDCDVIQ
CCHHCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASISGAWIALRLAVSGLMEQGQIKDDPITGKIAAISCGIYNGTPVLDLDYAED
CCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCC
SNADADANFVLIEGGKIAEAQATAEGATYDEEGLMRLLRLAQIGCAQIFKSQDEATK
CCCCCCCCEEEEECCCEECHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRPSGRAPDEMRAITIETGYTKHAEGSCLIGFGDTKVLCTASVEERLPPWLRGKGQGWVT
CCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCEEEEEECHHHHCCCCCCCCCCEEEE
GEYSMLPRATHTRGNREAARGKQSGRTQEIQRLIGRSLRAVVDLEKLGERQITLDCDVIQ
CCHHCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASISGAWIALRLAVSGLMEQGQIKDDPITGKIAAISCGIYNGTPVLDLDYAED
CCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCC
SNADADANFVLIEGGKIAEAQATAEGATYDEEGLMRLLRLAQIGCAQIFKSQDEATK
CCCCCCCCEEEEECCCEECHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA