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The map label for this gene is gyrB [H]

Identifier: 85374021

GI number: 85374021

Start: 1235319

End: 1237295

Strand: Reverse

Name: gyrB [H]

Synonym: ELI_05970

Alternate gene names: 85374021

Gene position: 1237295-1235319 (Counterclockwise)

Preceding gene: 85374022

Following gene: 85374018

Centisome position: 40.54

GC content: 62.27

Gene sequence:

>1977_bases
ATGTCCGACGACCTGTTTGAAAATACCCCCGCCTCCAGCGGCGATTACGATAGCTCTTCCATCGAGGTTCTCGAGGGCTT
GGAGCCCGTCCGCCGGCGCCCGGGCATGTATATTGGCGGTACCGACGATCGCGCATTGCATCACCTCGTCGCGGAGGTTC
TCGACAATGCGATGGACGAAGCGGTGGCGGGTCATGCCAGCCGGATCGCGATCACTCTGGAGGAAGGCAACACCGTCTCT
GTCGCGGACAACGGCCGCGGGATCCCTGTCGACGAGCATCCCAAGTTCCCCGGCAAGTCGACGCTGGAAGTGATCCTCTC
CACCCTGCACTCGGGCGGAAAGTTTTCGGGCAAGGCCTATGCGACGTCCGGCGGCCTTCACGGCGTAGGTGTCAGCGTCG
TCAATGCCCTGTCGAGCTACACCCGCGTGGAGGTGGCGCGCGACCGCGAGCTGTACGCGCAGGAATTTTCCAAGGGGCAG
GCGCTCGGCAAGATCGAGAAGCTCGGCCCGACGCCAAATCGGCGTGGCACCACTGTTACCTTCACACCCGACACTGAGAT
ATTCGGCGATCGCCAGTTCAAGCCTGCGCGGCTGTTCAAGCTCGCCCGCTCGAAGGCCTATCTCTTCGCGGGCGTCGAGA
TCCGCTGGAAATGCGCGCCCTCGCTGGCGAGCGATGACGTGCCCGAGGAGGCTGTGTTCAAATTTCCAGGCGGGCTAGCC
GATCACTTGAAGGAGCAGGTCGGCGAACGCGAATGCGTCACCGCCGACCCGTTCACCGGCAGCCAGGATTTCCCGGACAA
TCACGGCCGCGTCGAATGGGCGATCGCCTGGCCGCTCTACTCGGACGGTTCGACCAGCTGGTATTGCAACACCGTACCCA
CCCCCGATGGCGGGACGCATGAGCAAGGCCTGCGCGCCGCGCTCACCAAAGGCTTGCGGGTATTCGGCGAGCTGACCGGA
ACCAAGAAGGCCAAGGATATCTCCGCCGACGATGTGATGACCGGCGCTGAGATCATGCTCAGCGTGTTCATCCGCGATCC
GCAGTTCCAGTCGCAGACCAAGGACCGGCTGACGTCGCCCGAGGCCTCGCGCCTGGTCGAAAACGCGGTGCGCGATCACT
TCGATCATTTCCTGTCGGACAATATGGAGCGCGGCAAGGCGCTGCTCGGCTCGGTGATGGAGCGGATGGAGGAGCGGCTG
CGGCGTAAGCAGGAACGCGAGATCAAGCGCAAGACCGCGACCAATGCGAAAAAGCTGCGCCTGCCCGGTAAATTGACCGA
TTGCAGCGGCGAAACCGATGCCGAGACCGAGCTCTTCATCGTTGAAGGCGATTCCGCTGGCGGCAGCGCCAAACAGGCGC
GCGACCGCAAGACTCAGGCAATCCTGCCGATCCGCGGCAAGATCCTGAACGTCGCCAGCGCAACTGCCGACAAGATCCGC
GCCAACCAGGAAATTGCCGACCTCTCGCTCGCCATGGGCTGCGGCACGCGCAAGGATTGCGATCCCGAAAACCTGCGCTA
CGACCGCATCATCATCATGACCGATGCCGATGTCGACGGCGCGCACATCGCGACGCTGCTGATGACATTCTTCTTCCAGG
AAATGTCCGAGGTCGTTCGCAACGGGCATCTCTACCTCGCGCAACCTCCGCTCTATCGCCTGACTGCCGGAAAAGAGAGC
CGCTACGCGCAAGATGAAGCGCATCGGCAGGAACTTGAGGGTACGGTGTTCAAGGGCAAGAAAGTGGACGTGGCACGCTT
CAAGGGCCTCGGCGAGATGAACCCGCAACAGCTGCGCGAAACGACCATGAACCCGGAGACGCGCTCACTCATCCGCATTA
CTCTGCCGGCCGAGCATGAGCAGCGCTTTGCCGTGAAAGAACTGGTCGATCGCCTGATGGGCCGCAACCCCGAACATCGG
TTCAACTTCATTCAGAACCGCGCGGGTGAGATCGACCGGGATTTGATCGACGCTTAA

Upstream 100 bases:

>100_bases
CGCGCGTTTCGACCGGTAGCGCCGTCACTGCGCCGGGATTGCGCAAAACCCATCTCGCCTCCTTGTAGCCGCCTGAATCG
CGGGCCTATAGCCTTGTCCC

Downstream 100 bases:

>100_bases
AGCGCGCCGACGTCGAGCTTCTCGGCCGCGAACGCTTCGATCACTGGTTCAGAGTTGGCAAAAGTGCCCATCTTCGCCAT
GAACTGACGCGAGGTCGGCA

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 658; Mature: 657

Protein sequence:

>658_residues
MSDDLFENTPASSGDYDSSSIEVLEGLEPVRRRPGMYIGGTDDRALHHLVAEVLDNAMDEAVAGHASRIAITLEEGNTVS
VADNGRGIPVDEHPKFPGKSTLEVILSTLHSGGKFSGKAYATSGGLHGVGVSVVNALSSYTRVEVARDRELYAQEFSKGQ
ALGKIEKLGPTPNRRGTTVTFTPDTEIFGDRQFKPARLFKLARSKAYLFAGVEIRWKCAPSLASDDVPEEAVFKFPGGLA
DHLKEQVGERECVTADPFTGSQDFPDNHGRVEWAIAWPLYSDGSTSWYCNTVPTPDGGTHEQGLRAALTKGLRVFGELTG
TKKAKDISADDVMTGAEIMLSVFIRDPQFQSQTKDRLTSPEASRLVENAVRDHFDHFLSDNMERGKALLGSVMERMEERL
RRKQEREIKRKTATNAKKLRLPGKLTDCSGETDAETELFIVEGDSAGGSAKQARDRKTQAILPIRGKILNVASATADKIR
ANQEIADLSLAMGCGTRKDCDPENLRYDRIIIMTDADVDGAHIATLLMTFFFQEMSEVVRNGHLYLAQPPLYRLTAGKES
RYAQDEAHRQELEGTVFKGKKVDVARFKGLGEMNPQQLRETTMNPETRSLIRITLPAEHEQRFAVKELVDRLMGRNPEHR
FNFIQNRAGEIDRDLIDA

Sequences:

>Translated_658_residues
MSDDLFENTPASSGDYDSSSIEVLEGLEPVRRRPGMYIGGTDDRALHHLVAEVLDNAMDEAVAGHASRIAITLEEGNTVS
VADNGRGIPVDEHPKFPGKSTLEVILSTLHSGGKFSGKAYATSGGLHGVGVSVVNALSSYTRVEVARDRELYAQEFSKGQ
ALGKIEKLGPTPNRRGTTVTFTPDTEIFGDRQFKPARLFKLARSKAYLFAGVEIRWKCAPSLASDDVPEEAVFKFPGGLA
DHLKEQVGERECVTADPFTGSQDFPDNHGRVEWAIAWPLYSDGSTSWYCNTVPTPDGGTHEQGLRAALTKGLRVFGELTG
TKKAKDISADDVMTGAEIMLSVFIRDPQFQSQTKDRLTSPEASRLVENAVRDHFDHFLSDNMERGKALLGSVMERMEERL
RRKQEREIKRKTATNAKKLRLPGKLTDCSGETDAETELFIVEGDSAGGSAKQARDRKTQAILPIRGKILNVASATADKIR
ANQEIADLSLAMGCGTRKDCDPENLRYDRIIIMTDADVDGAHIATLLMTFFFQEMSEVVRNGHLYLAQPPLYRLTAGKES
RYAQDEAHRQELEGTVFKGKKVDVARFKGLGEMNPQQLRETTMNPETRSLIRITLPAEHEQRFAVKELVDRLMGRNPEHR
FNFIQNRAGEIDRDLIDA
>Mature_657_residues
SDDLFENTPASSGDYDSSSIEVLEGLEPVRRRPGMYIGGTDDRALHHLVAEVLDNAMDEAVAGHASRIAITLEEGNTVSV
ADNGRGIPVDEHPKFPGKSTLEVILSTLHSGGKFSGKAYATSGGLHGVGVSVVNALSSYTRVEVARDRELYAQEFSKGQA
LGKIEKLGPTPNRRGTTVTFTPDTEIFGDRQFKPARLFKLARSKAYLFAGVEIRWKCAPSLASDDVPEEAVFKFPGGLAD
HLKEQVGERECVTADPFTGSQDFPDNHGRVEWAIAWPLYSDGSTSWYCNTVPTPDGGTHEQGLRAALTKGLRVFGELTGT
KKAKDISADDVMTGAEIMLSVFIRDPQFQSQTKDRLTSPEASRLVENAVRDHFDHFLSDNMERGKALLGSVMERMEERLR
RKQEREIKRKTATNAKKLRLPGKLTDCSGETDAETELFIVEGDSAGGSAKQARDRKTQAILPIRGKILNVASATADKIRA
NQEIADLSLAMGCGTRKDCDPENLRYDRIIIMTDADVDGAHIATLLMTFFFQEMSEVVRNGHLYLAQPPLYRLTAGKESR
YAQDEAHRQELEGTVFKGKKVDVARFKGLGEMNPQQLRETTMNPETRSLIRITLPAEHEQRFAVKELVDRLMGRNPEHRF
NFIQNRAGEIDRDLIDA

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=623, Percent_Identity=25.3611556982343, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI19913408, Length=674, Percent_Identity=24.0356083086053, Blast_Score=122, Evalue=9e-28,
Organism=Escherichia coli, GI1789408, Length=638, Percent_Identity=42.9467084639498, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI48994957, Length=563, Percent_Identity=44.2273534635879, Blast_Score=422, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17535065, Length=588, Percent_Identity=24.1496598639456, Blast_Score=122, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI212645845, Length=344, Percent_Identity=23.8372093023256, Blast_Score=86, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324241, Length=651, Percent_Identity=25.3456221198157, Blast_Score=120, Evalue=5e-28,
Organism=Drosophila melanogaster, GI17136538, Length=588, Percent_Identity=24.3197278911565, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72814; Mature: 72683

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00018 EF_HAND_1 ; PS00626 RCC1_2 ; PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDLFENTPASSGDYDSSSIEVLEGLEPVRRRPGMYIGGTDDRALHHLVAEVLDNAMDE
CCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHH
AVAGHASRIAITLEEGNTVSVADNGRGIPVDEHPKFPGKSTLEVILSTLHSGGKFSGKAY
HHCCCCEEEEEEEECCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEE
ATSGGLHGVGVSVVNALSSYTRVEVARDRELYAQEFSKGQALGKIEKLGPTPNRRGTTVT
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEE
FTPDTEIFGDRQFKPARLFKLARSKAYLFAGVEIRWKCAPSLASDDVPEEAVFKFPGGLA
ECCCCCCCCCCCCCHHHHHHHHHCCEEEEECEEEEEEECCCCCCCCCCHHHHHHCCCCHH
DHLKEQVGERECVTADPFTGSQDFPDNHGRVEWAIAWPLYSDGSTSWYCNTVPTPDGGTH
HHHHHHHCCCCCEECCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCEEEECCCCCCCCCH
EQGLRAALTKGLRVFGELTGTKKAKDISADDVMTGAEIMLSVFIRDPQFQSQTKDRLTSP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
EASRLVENAVRDHFDHFLSDNMERGKALLGSVMERMEERLRRKQEREIKRKTATNAKKLR
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LPGKLTDCSGETDAETELFIVEGDSAGGSAKQARDRKTQAILPIRGKILNVASATADKIR
CCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCEECCCCCCCHHHHHHHHHHHH
ANQEIADLSLAMGCGTRKDCDPENLRYDRIIIMTDADVDGAHIATLLMTFFFQEMSEVVR
CCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
NGHLYLAQPPLYRLTAGKESRYAQDEAHRQELEGTVFKGKKVDVARFKGLGEMNPQQLRE
CCCEEEECCCCEEEECCCCHHHHHHHHHHHHHCCCEECCCCCCHHHHCCCCCCCHHHHHH
TTMNPETRSLIRITLPAEHEQRFAVKELVDRLMGRNPEHRFNFIQNRAGEIDRDLIDA
HCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHCC
>Mature Secondary Structure 
SDDLFENTPASSGDYDSSSIEVLEGLEPVRRRPGMYIGGTDDRALHHLVAEVLDNAMDE
CCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHH
AVAGHASRIAITLEEGNTVSVADNGRGIPVDEHPKFPGKSTLEVILSTLHSGGKFSGKAY
HHCCCCEEEEEEEECCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEE
ATSGGLHGVGVSVVNALSSYTRVEVARDRELYAQEFSKGQALGKIEKLGPTPNRRGTTVT
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEE
FTPDTEIFGDRQFKPARLFKLARSKAYLFAGVEIRWKCAPSLASDDVPEEAVFKFPGGLA
ECCCCCCCCCCCCCHHHHHHHHHCCEEEEECEEEEEEECCCCCCCCCCHHHHHHCCCCHH
DHLKEQVGERECVTADPFTGSQDFPDNHGRVEWAIAWPLYSDGSTSWYCNTVPTPDGGTH
HHHHHHHCCCCCEECCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCEEEECCCCCCCCCH
EQGLRAALTKGLRVFGELTGTKKAKDISADDVMTGAEIMLSVFIRDPQFQSQTKDRLTSP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
EASRLVENAVRDHFDHFLSDNMERGKALLGSVMERMEERLRRKQEREIKRKTATNAKKLR
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LPGKLTDCSGETDAETELFIVEGDSAGGSAKQARDRKTQAILPIRGKILNVASATADKIR
CCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCEECCCCCCCHHHHHHHHHHHH
ANQEIADLSLAMGCGTRKDCDPENLRYDRIIIMTDADVDGAHIATLLMTFFFQEMSEVVR
CCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
NGHLYLAQPPLYRLTAGKESRYAQDEAHRQELEGTVFKGKKVDVARFKGLGEMNPQQLRE
CCCEEEECCCCEEEECCCCHHHHHHHHHHHHHCCCEECCCCCCHHHHCCCCCCCHHHHHH
TTMNPETRSLIRITLPAEHEQRFAVKELVDRLMGRNPEHRFNFIQNRAGEIDRDLIDA
HCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9797224 [H]