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The map label for this gene is cysK [H]

Identifier: 85374007

GI number: 85374007

Start: 1220620

End: 1221540

Strand: Reverse

Name: cysK [H]

Synonym: ELI_05900

Alternate gene names: 85374007

Gene position: 1221540-1220620 (Counterclockwise)

Preceding gene: 85374008

Following gene: 85374002

Centisome position: 40.02

GC content: 64.06

Gene sequence:

>921_bases
ATGAAAGCCGACACCATCCTTCAAACCATCGGCAACACGCCGCATATCCGCATGAGCAAGCTGTTTCCGGACCATGAGGT
CTGGATCAAGTCGGAGCGGGCCAATCCCGGCGGATCGATCAAAGACCGCATTGCGCTCGCCATGATCGAGGATGCGGAAG
CTTCCGGCGCGCTCAAGCCGGGGGGCACGATCATCGAACCGACCAGCGGCAACACCGGCATCGGCCTCGCCATGGTCGCT
GCGGTCAAGGGCTACAAGCTCGTGCTGGTCATGCCTGAAAGCATGAGCCTCGAGCGCCGCCGCCTGATGCTGGCCTATGG
TGCCAGCTTCGACCTGACCGACAAGGCCAGGGGCATGAAGGGCGCGATCGAGCGCGCGACCGAGCTTGTCGCGCAGACCG
ACGGCGCATGGATGCCGAGCCAGTTCGACAACGAGAGCAACTGGAAGGTCCACGTTCGGACAACGGCCAAGGAAATCCTC
GAGGACTTCAAGGATGCGCCGATCGATGTGATGATCACCGGCGTCGGCACGGGCGGCCACCTGACCGGCTGCGCCGAAGA
GCTGAAGCGCCACTGGAGCGGCATGAAGGCCTATGGTGTCGAGCCCGAGCTCTCCCCCGTCATCAATGGCGGCCAGCCCG
GCCCGCACCCGATCCAAGGCATCGGCGCAGGCTTCATCCCAGGCAACCTGCACACACAATCGATCGACGGCGCGATCCAG
GTCGATGCCGAGGCTGCCAAGGACATGGCCCGCCGCGCCGCTGCCGAGGAAGGCATGCTGGTGGGTATTTCGAGCGGCGC
GACGCTGGCGGCGATTGCGAAAAAGCTGCCGGAACTTGAGGCTGGCTCACGGGTGCTGGGCTTCAACTACGATACCGGCG
AGCGGTATCTGAGCGTGCCGGACTTTCTACCGGAGGAGTGA

Upstream 100 bases:

>100_bases
ACGGCTGTTTCCCGTGCTGCGGACAACGAAGACTTTCGATCCGCCGCCGGAATTGTCTAAGAGTGATTCGTAACAGAACC
GACCCCCGGGAGAATACGCC

Downstream 100 bases:

>100_bases
GCGGTCTCCGCAAATGAAGAGGGCCCGCATCGCTGCGAGCCCTCTTGCGTGACGATCCTTGAATGCGATCAGGCGGCGGT
GGCGAAGGCCTCTTCGCCCA

Product: cysteine synthase K/M:cysteine synthase A

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKADTILQTIGNTPHIRMSKLFPDHEVWIKSERANPGGSIKDRIALAMIEDAEASGALKPGGTIIEPTSGNTGIGLAMVA
AVKGYKLVLVMPESMSLERRRLMLAYGASFDLTDKARGMKGAIERATELVAQTDGAWMPSQFDNESNWKVHVRTTAKEIL
EDFKDAPIDVMITGVGTGGHLTGCAEELKRHWSGMKAYGVEPELSPVINGGQPGPHPIQGIGAGFIPGNLHTQSIDGAIQ
VDAEAAKDMARRAAAEEGMLVGISSGATLAAIAKKLPELEAGSRVLGFNYDTGERYLSVPDFLPEE

Sequences:

>Translated_306_residues
MKADTILQTIGNTPHIRMSKLFPDHEVWIKSERANPGGSIKDRIALAMIEDAEASGALKPGGTIIEPTSGNTGIGLAMVA
AVKGYKLVLVMPESMSLERRRLMLAYGASFDLTDKARGMKGAIERATELVAQTDGAWMPSQFDNESNWKVHVRTTAKEIL
EDFKDAPIDVMITGVGTGGHLTGCAEELKRHWSGMKAYGVEPELSPVINGGQPGPHPIQGIGAGFIPGNLHTQSIDGAIQ
VDAEAAKDMARRAAAEEGMLVGISSGATLAAIAKKLPELEAGSRVLGFNYDTGERYLSVPDFLPEE
>Mature_306_residues
MKADTILQTIGNTPHIRMSKLFPDHEVWIKSERANPGGSIKDRIALAMIEDAEASGALKPGGTIIEPTSGNTGIGLAMVA
AVKGYKLVLVMPESMSLERRRLMLAYGASFDLTDKARGMKGAIERATELVAQTDGAWMPSQFDNESNWKVHVRTTAKEIL
EDFKDAPIDVMITGVGTGGHLTGCAEELKRHWSGMKAYGVEPELSPVINGGQPGPHPIQGIGAGFIPGNLHTQSIDGAIQ
VDAEAAKDMARRAAAEEGMLVGISSGATLAAIAKKLPELEAGSRVLGFNYDTGERYLSVPDFLPEE

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=305, Percent_Identity=40.655737704918, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI295821200, Length=305, Percent_Identity=40.655737704918, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI4557415, Length=305, Percent_Identity=40.655737704918, Blast_Score=197, Evalue=1e-50,
Organism=Escherichia coli, GI1788754, Length=305, Percent_Identity=48.1967213114754, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI2367138, Length=300, Percent_Identity=38, Blast_Score=191, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI115535073, Length=299, Percent_Identity=46.4882943143813, Blast_Score=218, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI17535051, Length=296, Percent_Identity=43.2432432432432, Blast_Score=215, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI32566674, Length=293, Percent_Identity=39.9317406143345, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI17562970, Length=294, Percent_Identity=39.4557823129252, Blast_Score=203, Evalue=8e-53,
Organism=Caenorhabditis elegans, GI17561720, Length=295, Percent_Identity=41.0169491525424, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17534315, Length=281, Percent_Identity=39.1459074733096, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI25147552, Length=278, Percent_Identity=36.6906474820144, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI32566672, Length=209, Percent_Identity=37.3205741626794, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17561716, Length=112, Percent_Identity=39.2857142857143, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321594, Length=315, Percent_Identity=34.6031746031746, Blast_Score=162, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6321449, Length=328, Percent_Identity=33.5365853658537, Blast_Score=119, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24643623, Length=306, Percent_Identity=35.6209150326797, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI20129101, Length=306, Percent_Identity=35.6209150326797, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32620; Mature: 32620

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKADTILQTIGNTPHIRMSKLFPDHEVWIKSERANPGGSIKDRIALAMIEDAEASGALKP
CCHHHHHHHHCCCCCEEEHHCCCCCCEEEEECCCCCCCCHHHHEEEEEEECCCCCCCCCC
GGTIIEPTSGNTGIGLAMVAAVKGYKLVLVMPESMSLERRRLMLAYGASFDLTDKARGMK
CCEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHEEEEEECCCCCCCHHHCCHH
GAIERATELVAQTDGAWMPSQFDNESNWKVHVRTTAKEILEDFKDAPIDVMITGVGTGGH
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCEEEEEEECCCCCC
LTGCAEELKRHWSGMKAYGVEPELSPVINGGQPGPHPIQGIGAGFIPGNLHTQSIDGAIQ
HHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEE
VDAEAAKDMARRAAAEEGMLVGISSGATLAAIAKKLPELEAGSRVLGFNYDTGERYLSVP
ECHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCCCCEEEEEECCCCCEEEECC
DFLPEE
CCCCCC
>Mature Secondary Structure
MKADTILQTIGNTPHIRMSKLFPDHEVWIKSERANPGGSIKDRIALAMIEDAEASGALKP
CCHHHHHHHHCCCCCEEEHHCCCCCCEEEEECCCCCCCCHHHHEEEEEEECCCCCCCCCC
GGTIIEPTSGNTGIGLAMVAAVKGYKLVLVMPESMSLERRRLMLAYGASFDLTDKARGMK
CCEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHEEEEEECCCCCCCHHHCCHH
GAIERATELVAQTDGAWMPSQFDNESNWKVHVRTTAKEILEDFKDAPIDVMITGVGTGGH
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCEEEEEEECCCCCC
LTGCAEELKRHWSGMKAYGVEPELSPVINGGQPGPHPIQGIGAGFIPGNLHTQSIDGAIQ
HHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEE
VDAEAAKDMARRAAAEEGMLVGISSGATLAAIAKKLPELEAGSRVLGFNYDTGERYLSVP
ECHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCCCCEEEEEECCCCCEEEECC
DFLPEE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8867384 [H]