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The map label for this gene is carA [H]

Identifier: 85373964

GI number: 85373964

Start: 1176001

End: 1177308

Strand: Direct

Name: carA [H]

Synonym: ELI_05685

Alternate gene names: 85373964

Gene position: 1176001-1177308 (Clockwise)

Preceding gene: 85373952

Following gene: 85373965

Centisome position: 38.53

GC content: 66.13

Gene sequence:

>1308_bases
ATGGCATCTGCCGACATTACGCCTGCGCAACCCAAAGACGCCACAGGTGTATTGCTTCTGGCCGATGGAACGGCGCTGTG
GGGCAAGGGCTTTGGGGCGACCGGCAGCGCGGTCGGCGAGGTGTGCTTCAACACCGCGATGACCGGCTACCAGGAGGTGA
TGACCGATCCCTCCTACGCGGCGCAGATCGTCACCTTCACCTTCCCGCATATCGGCAATGTCGGCTCGAACGACGAGGAC
GTCGAAAGCGCCGTCGAAGCGGCTGTCGGCTGCGTGGTGCGCGAGGAAGTCACGCCGTCGAGCAATTTCCGCGCCGACAA
GCACTTCGACGAATGGATGAGCGCCAATGGCAAGATCGGCCTTTCCGGCATCGATACCCGCGCGCTGACCCGCTTCATCC
GGCTAAGCGGCGCGCCCAACGCTGTGATCGCGCATGCTCCCGACGGAACATTCGACCTGCCTGCACTGCTCGCCCGCGCG
CAAAAGTGGCCGGGGCTGGAGGGCATGGACCTCGCCCAGCGCGTCAGCCGCGCGAAGCAGGAAGATTGGGGCGGGGGTCC
GTGGGCTCTCGGCAAGGGCTACGGGCGAGCGCCGTGGCCGAAATCGCCCTCTCTCCTTCAGGGGAGAGGGAGGAGCGACG
AAGTCGCGGAGGGTGTGGGGGATGCTCGCGACGCATCGCCAGCCAGCCACCACCCCCCGACCCCCTCCTCTGAAGAAGAG
GGGGAGTTTATGCCTCACGTGGTCGCGATCGACTACGGCGCGAAGGACAATATCTTCCGCAACCTGGTGAAGGCCGGGGC
GCGGGTGACGGTGGTGCCGGCCAAGGCCAGCCTCGAAGAGGTGCTGGCGCACAATCCCGACGGCGTGTTCCTGTCCAACG
GTCCGGGCGATCCGGCGGCGACGGGCGAATATGCGGTGCCGGTGATTGCCGGGTTGCTGGAGCGCGACATCCCGCTGTTC
GGCATCTGCCTCGGCCACCAGATGCTGGCGCTGGCGGCGGGCGCGAAAACCACCAAGATGCACCAGGGCCACCGCGGCGC
GAACCACCCGGTCCAGCGGGTGGGCGAAGGCTGGGGCGAGACCTCGGGCCTCGTCGAGATCACCAGCATGAACCACGGCT
TCGCGGTCGATGGCGAGACTTTGCCGGAGGGTGTGGAGCAGACGCATGTGAGCCTGTTCGACGGCAGCAATTGCGGCATC
AGCATCCGCGGCAAGAAAGCGTTCGGCGTGCAGTATCACCCGGAGGCTAGCCCGGGACCGCAGGACAGCTTCTACCTGTT
CGAGAAGTTTGTGGGGATGTTGGGGTGA

Upstream 100 bases:

>100_bases
CCCCAAGTGGCGCGCGATCGCGCTTGACGCAAGGGGAGGGTGCGCTTAGCCGCAGTCTCTTAGCGACATCGCCGGAAACA
CCTGACTCGGAGCAAGCCCC

Downstream 100 bases:

>100_bases
GAAAAAAGCTAATCCTTTTGCTTGGAGCGTTGTCCCTAGTCGGGGTTTCTCCCGCAATGGCTCAGAAGCCGCCAGAACCT
GATGCGGCGTCTCCCCGCCA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MASADITPAQPKDATGVLLLADGTALWGKGFGATGSAVGEVCFNTAMTGYQEVMTDPSYAAQIVTFTFPHIGNVGSNDED
VESAVEAAVGCVVREEVTPSSNFRADKHFDEWMSANGKIGLSGIDTRALTRFIRLSGAPNAVIAHAPDGTFDLPALLARA
QKWPGLEGMDLAQRVSRAKQEDWGGGPWALGKGYGRAPWPKSPSLLQGRGRSDEVAEGVGDARDASPASHHPPTPSSEEE
GEFMPHVVAIDYGAKDNIFRNLVKAGARVTVVPAKASLEEVLAHNPDGVFLSNGPGDPAATGEYAVPVIAGLLERDIPLF
GICLGHQMLALAAGAKTTKMHQGHRGANHPVQRVGEGWGETSGLVEITSMNHGFAVDGETLPEGVEQTHVSLFDGSNCGI
SIRGKKAFGVQYHPEASPGPQDSFYLFEKFVGMLG

Sequences:

>Translated_435_residues
MASADITPAQPKDATGVLLLADGTALWGKGFGATGSAVGEVCFNTAMTGYQEVMTDPSYAAQIVTFTFPHIGNVGSNDED
VESAVEAAVGCVVREEVTPSSNFRADKHFDEWMSANGKIGLSGIDTRALTRFIRLSGAPNAVIAHAPDGTFDLPALLARA
QKWPGLEGMDLAQRVSRAKQEDWGGGPWALGKGYGRAPWPKSPSLLQGRGRSDEVAEGVGDARDASPASHHPPTPSSEEE
GEFMPHVVAIDYGAKDNIFRNLVKAGARVTVVPAKASLEEVLAHNPDGVFLSNGPGDPAATGEYAVPVIAGLLERDIPLF
GICLGHQMLALAAGAKTTKMHQGHRGANHPVQRVGEGWGETSGLVEITSMNHGFAVDGETLPEGVEQTHVSLFDGSNCGI
SIRGKKAFGVQYHPEASPGPQDSFYLFEKFVGMLG
>Mature_434_residues
ASADITPAQPKDATGVLLLADGTALWGKGFGATGSAVGEVCFNTAMTGYQEVMTDPSYAAQIVTFTFPHIGNVGSNDEDV
ESAVEAAVGCVVREEVTPSSNFRADKHFDEWMSANGKIGLSGIDTRALTRFIRLSGAPNAVIAHAPDGTFDLPALLARAQ
KWPGLEGMDLAQRVSRAKQEDWGGGPWALGKGYGRAPWPKSPSLLQGRGRSDEVAEGVGDARDASPASHHPPTPSSEEEG
EFMPHVVAIDYGAKDNIFRNLVKAGARVTVVPAKASLEEVLAHNPDGVFLSNGPGDPAATGEYAVPVIAGLLERDIPLFG
ICLGHQMLALAAGAKTTKMHQGHRGANHPVQRVGEGWGETSGLVEITSMNHGFAVDGETLPEGVEQTHVSLFDGSNCGIS
IRGKKAFGVQYHPEASPGPQDSFYLFEKFVGMLG

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI169790915, Length=427, Percent_Identity=31.3817330210773, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI21361331, Length=427, Percent_Identity=31.3817330210773, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI18105007, Length=193, Percent_Identity=44.559585492228, Blast_Score=142, Evalue=5e-34,
Organism=Escherichia coli, GI1786215, Length=422, Percent_Identity=46.9194312796209, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI193204318, Length=188, Percent_Identity=42.5531914893617, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6322331, Length=450, Percent_Identity=32.6666666666667, Blast_Score=199, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6324878, Length=194, Percent_Identity=45.360824742268, Blast_Score=146, Evalue=8e-36,
Organism=Drosophila melanogaster, GI45555749, Length=429, Percent_Identity=32.6340326340326, Blast_Score=175, Evalue=6e-44,
Organism=Drosophila melanogaster, GI24642586, Length=429, Percent_Identity=32.6340326340326, Blast_Score=174, Evalue=1e-43,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 45764; Mature: 45632

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASADITPAQPKDATGVLLLADGTALWGKGFGATGSAVGEVCFNTAMTGYQEVMTDPSYA
CCCCCCCCCCCCCCCEEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
AQIVTFTFPHIGNVGSNDEDVESAVEAAVGCVVREEVTPSSNFRADKHFDEWMSANGKIG
EEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEE
LSGIDTRALTRFIRLSGAPNAVIAHAPDGTFDLPALLARAQKWPGLEGMDLAQRVSRAKQ
CCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
EDWGGGPWALGKGYGRAPWPKSPSLLQGRGRSDEVAEGVGDARDASPASHHPPTPSSEEE
HCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCC
GEFMPHVVAIDYGAKDNIFRNLVKAGARVTVVPAKASLEEVLAHNPDGVFLSNGPGDPAA
CCCCCEEEEEECCCCHHHHHHHHHCCCEEEEEECHHHHHHHHHCCCCCEEEECCCCCCCC
TGEYAVPVIAGLLERDIPLFGICLGHQMLALAAGAKTTKMHQGHRGANHPVQRVGEGWGE
CCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHCCCCC
TSGLVEITSMNHGFAVDGETLPEGVEQTHVSLFDGSNCGISIRGKKAFGVQYHPEASPGP
CCCEEEEEECCCCEEECCCCCCCCHHHHEEEEEECCCCCEEEECCEEECEEECCCCCCCC
QDSFYLFEKFVGMLG
CCHHHHHHHHHHHCC
>Mature Secondary Structure 
ASADITPAQPKDATGVLLLADGTALWGKGFGATGSAVGEVCFNTAMTGYQEVMTDPSYA
CCCCCCCCCCCCCCEEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
AQIVTFTFPHIGNVGSNDEDVESAVEAAVGCVVREEVTPSSNFRADKHFDEWMSANGKIG
EEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEE
LSGIDTRALTRFIRLSGAPNAVIAHAPDGTFDLPALLARAQKWPGLEGMDLAQRVSRAKQ
CCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
EDWGGGPWALGKGYGRAPWPKSPSLLQGRGRSDEVAEGVGDARDASPASHHPPTPSSEEE
HCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCC
GEFMPHVVAIDYGAKDNIFRNLVKAGARVTVVPAKASLEEVLAHNPDGVFLSNGPGDPAA
CCCCCEEEEEECCCCHHHHHHHHHCCCEEEEEECHHHHHHHHHCCCCCEEEECCCCCCCC
TGEYAVPVIAGLLERDIPLFGICLGHQMLALAAGAKTTKMHQGHRGANHPVQRVGEGWGE
CCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHCCCCC
TSGLVEITSMNHGFAVDGETLPEGVEQTHVSLFDGSNCGISIRGKKAFGVQYHPEASPGP
CCCEEEEEECCCCEEECCCCCCCCHHHHEEEEEECCCCCEEEECCEEECEEECCCCCCCC
QDSFYLFEKFVGMLG
CCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA