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The map label for this gene is panB

Identifier: 85373896

GI number: 85373896

Start: 1106750

End: 1107619

Strand: Reverse

Name: panB

Synonym: ELI_05345

Alternate gene names: 85373896

Gene position: 1107619-1106750 (Counterclockwise)

Preceding gene: 85373899

Following gene: 85373895

Centisome position: 36.29

GC content: 64.6

Gene sequence:

>870_bases
ATGTCCACGACCTTCAAGCTCGATACGAGCACGAGCCGCGCCAATCCCACCCCCGCTCCGATGAAGCGGCTGACCGTGCC
GAAAATCCGCGCGCGGAAGAAAGACGGCAAGACCGAAGAGCCGCTGGTCATGCTGACGGCCTACACCGCGCGGCAGGCGC
AGCTGCTCGATGCCCATTGCGACCTGCTGCTGGTTGGGGATTCGCTGGGGCAGGTGATCTATGGTCTGCCCTCCACCATT
CCCGTCACCCTGCGGATGATGGCGGACCACGCAGCGGCGGTCGTACGCGGGAGCTATCACTCGGTGGTTGTGGTCGACAT
GCCTTTCGGATCCTACGAGCAATCGCCGCAGCAGGCCTTCGAGAGCGCCAGCTTCCTGCTCAAGGAAAGCGGCGCAGCGG
CGGTCAAGCTCGAAGGCGGCGAAGCGATGGCGGAGACGGTCGCATTTCTCAACCAGCGAGGCATCCCGGTGATGGGCCAT
GTCGGGCTGACCCCGCAAGCGGTCAATGTCCTCGGCGGCTATGCCGCGCGCGGCCGCAATGATGCGGAGGCCGACAAGAT
CGTCACCGACGCCAAGGCACTCGACGATGCCGGGGCCTTTGCCATCGTCATCGAAGGCGTGCTGGAGCCGATCGCCGTCG
CCGCCACCGAGGCCGTTCGCGCGCCGACGATCGGGATCGGTGCTTCGGCGCAGTGCGATGGGCAGGTGCTGGTGACGGAG
GACATGCTGGGCATGTTCGAACGCGTCCCGCGCTTTGTGAAGAAGTATGGCGATATCGCCGAAACCATCGAAGCGACGGC
CAAGACCTATGCCGAGGAAGTCCGCGCGAGAAGCTTCCCCGGACCAGAACAGCTTTACCAGCCCAAATAG

Upstream 100 bases:

>100_bases
GGGTAATGCGCGCCTTCTGCAACGATTTTGCGCCTAGGCCAAGGGCCAAAGGAAACGGGACGCTTTGCCTTTGCCGCCAC
GCGCGGCTAATCCACGCGCC

Downstream 100 bases:

>100_bases
GCTTCCGGCGCGCTTGCGAGCCTCAGGCAAATACCCTAGCGCGCGCCGCAAACGGGTTCAGGACAATCGATGCAAACGCT
GCTGCGCTACTACACTTTTT

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSTTFKLDTSTSRANPTPAPMKRLTVPKIRARKKDGKTEEPLVMLTAYTARQAQLLDAHCDLLLVGDSLGQVIYGLPSTI
PVTLRMMADHAAAVVRGSYHSVVVVDMPFGSYEQSPQQAFESASFLLKESGAAAVKLEGGEAMAETVAFLNQRGIPVMGH
VGLTPQAVNVLGGYAARGRNDAEADKIVTDAKALDDAGAFAIVIEGVLEPIAVAATEAVRAPTIGIGASAQCDGQVLVTE
DMLGMFERVPRFVKKYGDIAETIEATAKTYAEEVRARSFPGPEQLYQPK

Sequences:

>Translated_289_residues
MSTTFKLDTSTSRANPTPAPMKRLTVPKIRARKKDGKTEEPLVMLTAYTARQAQLLDAHCDLLLVGDSLGQVIYGLPSTI
PVTLRMMADHAAAVVRGSYHSVVVVDMPFGSYEQSPQQAFESASFLLKESGAAAVKLEGGEAMAETVAFLNQRGIPVMGH
VGLTPQAVNVLGGYAARGRNDAEADKIVTDAKALDDAGAFAIVIEGVLEPIAVAATEAVRAPTIGIGASAQCDGQVLVTE
DMLGMFERVPRFVKKYGDIAETIEATAKTYAEEVRARSFPGPEQLYQPK
>Mature_288_residues
STTFKLDTSTSRANPTPAPMKRLTVPKIRARKKDGKTEEPLVMLTAYTARQAQLLDAHCDLLLVGDSLGQVIYGLPSTIP
VTLRMMADHAAAVVRGSYHSVVVVDMPFGSYEQSPQQAFESASFLLKESGAAAVKLEGGEAMAETVAFLNQRGIPVMGHV
GLTPQAVNVLGGYAARGRNDAEADKIVTDAKALDDAGAFAIVIEGVLEPIAVAATEAVRAPTIGIGASAQCDGQVLVTED
MLGMFERVPRFVKKYGDIAETIEATAKTYAEEVRARSFPGPEQLYQPK

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=271, Percent_Identity=41.6974169741697, Blast_Score=193, Evalue=9e-51,
Organism=Saccharomyces cerevisiae, GI6319653, Length=285, Percent_Identity=38.5964912280702, Blast_Score=166, Evalue=5e-42,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_ERYLH (Q2NAY0)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457958.1
- ProteinModelPortal:   Q2NAY0
- SMR:   Q2NAY0
- STRING:   Q2NAY0
- GeneID:   3870737
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_05345
- NMPDR:   fig|314225.3.peg.1995
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   DMMIAHG
- PhylomeDB:   Q2NAY0
- ProtClustDB:   PRK00311
- BioCyc:   ELIT314225:ELI_05345-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 30782; Mature: 30650

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: ACT_SITE 205-205 BINDING 106-106 BINDING 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTFKLDTSTSRANPTPAPMKRLTVPKIRARKKDGKTEEPLVMLTAYTARQAQLLDAHC
CCCEEEEECCCCCCCCCCCCHHHCCCHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHCC
DLLLVGDSLGQVIYGLPSTIPVTLRMMADHAAAVVRGSYHSVVVVDMPFGSYEQSPQQAF
CEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHH
ESASFLLKESGAAAVKLEGGEAMAETVAFLNQRGIPVMGHVGLTPQAVNVLGGYAARGRN
HHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHCCCC
DAEADKIVTDAKALDDAGAFAIVIEGVLEPIAVAATEAVRAPTIGIGASAQCDGQVLVTE
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHH
DMLGMFERVPRFVKKYGDIAETIEATAKTYAEEVRARSFPGPEQLYQPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCC
>Mature Secondary Structure 
STTFKLDTSTSRANPTPAPMKRLTVPKIRARKKDGKTEEPLVMLTAYTARQAQLLDAHC
CCEEEEECCCCCCCCCCCCHHHCCCHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHCC
DLLLVGDSLGQVIYGLPSTIPVTLRMMADHAAAVVRGSYHSVVVVDMPFGSYEQSPQQAF
CEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHH
ESASFLLKESGAAAVKLEGGEAMAETVAFLNQRGIPVMGHVGLTPQAVNVLGGYAARGRN
HHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHCCCC
DAEADKIVTDAKALDDAGAFAIVIEGVLEPIAVAATEAVRAPTIGIGASAQCDGQVLVTE
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHH
DMLGMFERVPRFVKKYGDIAETIEATAKTYAEEVRARSFPGPEQLYQPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA